Effects of tributyltin at environmental levels on monooxygenase system of digestive gland in hard clam Meretrix meretrix

The effects on ethoxyresorufin O-deethylase （EROD）, NADPH-cytochrome c reductase and NADH cytochrorne b5 reductase activities of digestive gland in Meretrix meretrix exposed to tributyltin （TBT） at environmental levels （0.1,1.0,10.0 ng/dm as stannum concentration）,in experimental condition, were evaluated. The EROD, NADH cytochrorne b5 reductase activities were significantly inhibited after exposure to 10.0 ng/dm^3 TBT for 8 and 20 d, the NADPH cytochrorne c reductase activities were significantly inhibited after exposure to 0.1,1.0 and 10.0 ng/dm TBT for 8 d and to 1.0 and 10.0 ng/dm for 20 d, as compared with the matched control, while NADH cytochrorne b5 reductases and NADPH cytochrome c reductase activities were induced after exposure to 10.0 ng/dm^3 TBT for 2 d. The EROD activity in the 10 ng/dm^3 group,and the NADH cytochrome b5 reductases activities in 1.0 and 10.0 ng/dm groups, were significantly induced when transferred to clean recovery tanks for 7 d. The three enzymic activities in the clams exposed to TBT were recovered to the level corresponding to that of the control group after transfer to clean recovery tanks for 20 d. NADPH cytochrome c reductase activity in Meretrix meretrix seems to be more sensitive to exposure of TBT than that of the EROD and NADH cytochrome b5 reductases. The results suggest that induction and inhibition by TBT to the monooxygenase system enzymic activity in Meretrix meretrix would simultaneously exist. The enzymic activities were inhibited by exposure for a long time. The results suggest that inhibition of the monooxygenase system should be an indication of the exposure to environmentally relevant concentrations of TBT for a long time.     

无公害刺参稳产健康养殖新技术

本文针对目前刺参养殖中存在的问题,简单明了叙述刺参无公害健康养殖一系列新技术和新方法,对推动海参养殖业持续、稳定、健康发展,起到积极作用和效果。     

太平洋东边界波浪输运

通过计算2000年涌浪指标(swell index)的全球分布,发现太平洋东边界赤道附近区域存在涌浪池.利用ECMWF再分析波浪资料,计算出2000年全球月平均波浪体积输运.比较2000年全球月平均波浪体积输运和2000年QUICKSCAT月平均风场,发现在赤道太平洋东边界涌浪池区域内的波浪输运方向和风向存在很大的差别,两者方向相差大约90°.这进一步验证了该地区涌浪池存在的真实性.研究发现,赤道太平洋东边界涌浪主要来源于北太平洋和南太平洋的西风带对应的海区.在涌浪池区域内分别在2.5°S和2.5°N取两条边界(边界起点为125°W,终点为美洲大陆西边界),计算通过这两条边界进入赤道区域涌浪的Stokes体积净输运量.结果表明,不同月份通过南、北两条边界波浪的净输运量与当月南、北太平洋西风带的风浪强度密切相关.同时指出了,涌浪的体积输运将会对大洋环流系统产生潜在的重要影响.     

渤海湾天津近岸海域水环境空间变异分析

由于海岸带的地理条件和海洋动力特性的复杂性以及人类开发活动对海岸带的影响,近岸海域的生态环境系统在空间分布上有很大的变异性.基于现场实测资料,建立系统的空间变异分析模型,对海岸带生态环境空间变化规律进行分析,可以充分了解不同区域的污染特征和变化趋势.应用所构建的空间变异分析模型对渤海湾天津近岸海域的水环境特性进行分析,结合对应年份的入海流量及其变化,分析了天津渤海湾海岸带污染的主因子和区域水环境的分布特征,结果可以对海洋功能区的划分和水环境综合评价结果进行验证和补充.     

神舟四号高度计波形数据预处理和信息提取

神舟四号(SZ-4)高度计在国内首次提供了星载雷达高度计回波波形数据.本文中作者分析了SZ-4高度计回波波形的特点,完成波形数据的预处理,并在此基础上完成初步的信息提取.在数据预处理方面,通过SZ-4高度计水陆边界处波形的特点,提出了波形最大幅度控制的方法,筛选回波波形.在波形归一化处理过程中,发现SZ-4高度计波形中存在双峰现象,并指出第二个峰为异常波形区.在波形信息提取方面,利用波形重新跟踪得到的半功率点计算出SZ-4高度计高度跟踪补偿误差,并根据高度计天线指向角和回波波形下降沿斜率之间的关系,从波形后沿提取天线指向角信息.分析结果表明,SZ-4高度计天线指向比较平稳,而跟踪补偿由于变化较大,在计算海面高度时,应作为一项误差源被考虑到.     

Photoinduced toxicity single and binary mixtures of four polycyclic aromatic hydrocarbons to the marine diatom Skeletonema costatum

Diatom Skeletonema costatum Cleve is one of the main predominant phytoplankton species in the Changjiang Estuary in China. In order to provide some basic information for future assessment of the potential risk on phytoplankton communities in this estuary caused by polycyclic aromatic hydrocarbons(PAHs), this alga was selected as a representative to investigate the photoinduced toxicity of PAHs, in single and mixture. Four PAHs including three-ring phenanthrene and anthracene, four-ring fluoranthene and pyrene were tested in the laboratory. The single toxicity of each PAH on this microalga was compared with and without the simulated solar UV radiation. The results showed that this microalga was sensitive to PAH's photoinduced toxicity. Ratios of the 72 h median effect concentration obtained for fluorescent and UV light tests were about 8.4 for phenanthrene, 13.0 for anthracene, 6.5 for fluoranthene, and 5.7 for pyrene, indicating that UV light enhanced the PAH toxicity to this alga significantly. Under the fluorescent radiation (lacking UV), the dose-response curves based on chemical concentrations revealed that the order of toxic strength was fluoranthene greater than pyrene greater than anthracene greater than phenanthrene; while under the UV radiation (476 μW/cm2 for UVA, 6.5 μW/cm2 for UVB) it became fluoranthene approximately equaling anthracene greater than pyrene greater than phenanthrene, indicating that the UV light also changed its relative toxicity to this alga. The photoinduced toxicity of PAHs to the marine diatom S. costatum might be a synergistic effect of photosensitization reactions (e.g., generation of single-state oxygen) and photomodification (photooxidation and/or photolysis).The combined effects of six binary mixtures on the marine diatom S. costatum were investigated using the additive-index method. Four binary-mixtures (phenanthrene plus anthracene; phenanthrene plus pyrene; anthracene plus fluoranthene; anthracene plus pyrene) were found to be synergistic joint action mode, while two binary-mixtures (phenanthrene plus fluoranthene; fluoranthene plus pyrene) displayed antagonist, revealing a complex pattern of possible interactions. The combined action mode of PAHs might be related to various factors such as the mixture compounds, mixture ratios and test conditions, etc.     

Phylogenetic analysis of Pectinidae(Bivalvia) based on the ribosomal DNA internal transcribed spacer region

The ribosomal DNA internal transcribed spacer (ITS) region is a useful genomic region for understanding evolutionary and genetic relationships. In the current study, the molecular phylogenetic analysis of Pectinidae (Mollusca: Bivalvia) was performed using the nucleotide sequences of the nuclear ITS region in nine species of this family. The sequences were obtained from the scallop species Argopecten irradians, Mizuhopecten yessoensis, Amusium pleuronectes and Mimachlamys nobilis, and compared with the published sequences of Aequipecten opercularis, Chlamys farreri, C. distorta, M. varia, Pecten maximus, and an outgroup species Perna viridis. The molecular phylogenetic tree was constructed by the neighbor-joining and maximum parsimony methods. Phylogenetic analysis based on ITS1, ITS2, or their combination always yielded trees of similar topology. The results support the morphological classifications of bivalve and are nearly consistent with classification of two subfamilies (Chlamydinae and Pectininae) formulated by Waller. However, A. irradians, together with A. opercularis made up of genera Amusium, evidences that they may belong to the subfamily Pectinidae. The data are incompatible with the conclusion of Waller who placed them in Chlamydinae by morphological characteristics. These results provide new insights into the evolutionary relationships among scallop species and contribute to the improvement of existing classification systems.     

Velocity structure in the South Yellow Sea basin based on first-arrival tomography of wide-angle seismic data and its geological implications

The South Yellow Sea basin is filled with Mesozoic–Cenozoic continental sediments overlying pre-Palaeozoic and Mesozoic–Palaeozoic marine sediments. Conventional multi-channel seismic data cannot describe the velocity structure of the marine residual basin in detail, leading to the lack of a deeper understanding of the distribution and lithology owing to strong energy shielding on the top interface of marine sediments. In this study, we present seismic tomography data from ocean bottom seismogra...     

Comparison of short-term toxicity of 14 common phycotoxins (alone and in combination) to the survival of brine shrimp Artemia salina

Toxic harmful algal blooms(HABs) can cause deleterious effects in marine organisms, threatening the stability of marine ecosystems. It is well known that different strains, natural populations and growth conditions of the same toxic algal species may lead to different amount of phycotoxin production and the ensuing toxicity. To fully assess the ecological risk of toxic HABs, it is of great importance to investigate the toxic effects of phycotoxins in marine organisms. In this study, the short-te...