基于网络药理学与体外细胞实验探讨复方清痹片治疗类风湿性关节炎的作用机制

目的：基于网络药理学与体外细胞实验探讨复方清痹片治疗类风湿性关节炎(RA)的作用机制。方法：基于TCMID、TCMSP和DrugBank数据库筛选复方清痹片的活性成分并预测潜在活性成分靶点;基于GeneCard、OMIM等数据库筛选RA疾病靶点;明确复方清痹片活性成分与RA的共同潜在靶标;利用STRING数据库和Cytoscape软件构建蛋白质-蛋白质相互作用（PPI）网络,并通过DAVID数据库进行基因本体（GO）功能及京都基因与基因组百科全书（KEGG）通路富集分析,RT-PCR检测复方清痹片对肿瘤坏死因子（TNF-α）诱导的人类风湿性关节炎成纤维样滑膜细胞（HFLS-RA）模型白介素6（IL-6）、TNF-α、丝氨酸/苏氨酸蛋白激酶1（AKT1）和血管内皮生长因子A（VEGFA）mRNA表达的影响。结果：筛选出217种潜在活性成分以及158个潜在靶点;IL-6、TNF、AKT1、白蛋白（ALB）、VEGFA等是复方清痹片治疗RA的关键靶点;复方清痹片主要通过炎性因子、趋化因子、免疫调控等信号通路来发挥治疗RA的作用。复方清痹片能够抑制HFLS-RA增殖,下调IL-6、TNF、AKT1和VEGFA mRNA的表达,这与网络药理学预测的结果相一致。结论：本研究揭示了复方清痹片治疗RA“活性成分-靶点-通路”的复杂关系,为后续该药的临床应用提供了研究基础。     

Extraction of Gravity–Magnetic Anomalies Associated with Pb–Zn–Fe Polymetallic Mineralization in Luziyuan Ore Field,Yunnan Province,Southwestern China

The method of bi-dimensional empirical mode decomposition (BEMD) and the combined methods of entropy weight–Technique for Order of Preference by Similarity to an Ideal Solution (TOPSIS) were used to decompose gravity–magnetic data and evaluate targets in the Luziyuan Pb–Zn–Fe polymetallic ore field and surrounding areas. Three meaningful bi-dimensional intrinsic mode function (BIMF) images were obtained by BEMD at different wavelengths, depicting different layers of geological architectures in the study area. The results are as follows. (1) The BIMF2 images depict the shallow local geological architecture and show positive gravity–magnetic anomalies of the skarn alteration and Pb–Zn–Fe mineralization distributed around concealed granites. (2) The BIMF3 images depict the medium-depth geological architecture, indicating that concealed granitic stocks, which are shallow extensions of a deeply concealed pluton, intruded along the NE-trending fault. (3) The BIMF4 images depict gravity–magnetic anomalies at greater depth, which likely reflect regional geological architectures, indicating the potential presence of a large, concealed intermediate-acid pluton in the negative anomaly zone. Three potential targets (A, B, and C) were delineated based on BEMD results of the original gravity–magnetic data. The entropy weight–TOPSIS evaluation results show that the ranking of the metallogenic potential of the delineated targets in the study area is B, A, and C, with relative proximity values of 0.4576, 0.3925, and 0.1499, respectively. The results of this study can be used to guide future exploration.

     

Are creative workers happier in Chinese cities? The influence of work,lifestyle, and amenities on urban well-being

This paper examines two propositions in the creative-class debate in the context of China’s large cities. The first evaluates Richard Florida’s contention that creative workers enjoy higher level of well-being and are happier than blue-collar workers. The second investigates the influence of urban amenities, lifestyle, and work on creative workers’ happiness. Both propositions are analyzed in relation to China’s post-Socialist, industrial, and mining cities. Based on data from a household survey, the results indicate that Chinese creative workers are indeed happier in post-Socialist, or service-sector oriented, cities. However, creative work does not explain their happiness. Stable social sites that reinforce authentic experience of urban cultural amenities (e.g. the teahouse rather than modern coffeehouses, bars, and theaters) are a significant factor for creative-class happiness in post-Socialist Chinese cities. However, creative work is more likely to explain creative-class happiness in mining cities. Further, our study found that blue-collar workers report a higher level of happiness in those settings with less pronounced inter- and intra-class distinctions at the workplace—in those places where power and status are more evenly distributed.     

安基山铜矿含矿裂隙分布特征及其与成矿的关系

本文用定点等积测量法研究安基山铜矿地表含矿裂隙,结果表明:成矿期含矿裂隙集中分布在矿化斑岩中,其含脉率等值线与铜矿化强度率等值线基本吻合。各成矿阶段含矿裂隙及矿体展布方向均以北北西为主,平行于区域最大挤压应力方向。由此推断,斑岩体地表含脉率值可判断深部铜矿化强度,含脉率等值线形态可预测矿体主要延伸方向。     

应用模糊数学统计识别花岗岩古潜山裂缝的方法与效果

在裂缝带找油具有广阔的前景。本文从尝试用模糊数学统计方法来识别裂缝发育带入手,分别用一维,多维统计方法对辽河油田花岗岩古潜山的一部分样品进行了试算,结果较好。     

Functionality analysis of an urban water supply network after strong earthquakes

An urban water supply network(WSN)is a crucial lifeline system that helps to maintain the normal functioning of modern society.However,the hydraulic analysis of a significantly damaged WSN that suffers from pipe breaks or leaks remains challenging.In this paper,a probability-based framework is proposed to assess the functionality of WSNs in the aftermath of powerful earthquakes.The serviceability of the WSN is quantified by using a comprehensive index that considers nodal water flow and nodal pressure.This index includes a coefficient that reflects the relative importance of these two parameters.The demand reduction(DR)method,which reduces the water flow of nodes while preventing the negative pressure of nodes,is proposed.The difference between the negative pressure elimination(NPE)method and the DR method is discussed by using the example of a WSN in a medium-sized city in China.The functionality values of the WSN are 0.76 and 0.99 when nodal pressure and nodal demands are used respectively as the index of system serviceability at an intensity level that would pertain to an earthquake considered to occur at a maximum level.When the intensity of ground motion is as high as 0.4 g,the DR method requires fewer samples than the NPE method to obtain accurate results.The NPE method eliminates most of the pipes,which may be unrealistic.     

内蒙古锡林浩特盆地早侏罗世晚期植物群:兼论中国早侏罗世晚期气候

首次报道了产于内蒙古锡林浩特盆地侏罗系红旗组的植物群,共计21属37种。该植物群由木贼目、真蕨纲、苏铁纲、银杏纲、松柏纲和少量分散保存的种子等组成,以真蕨类丰富、蚌壳蕨科Coniopteris和Eboracia出现以及南方型分子较多等为特征。该植物群的时代为早侏罗世晚期,反映研究区红旗组主体的沉积时代为早侏罗世晚期,上部沉积可能形成于中侏罗世早期。气候敏感植物,特别是南方型分子的出现,指示锡林浩特盆地早侏罗世晚期属于暖温带—亚热带气候区,气候较热或短期有一定程度的偏干旱;其气温较早侏罗世早期和中侏罗世早期更高,说明早侏罗世晚期发生过1次升温气候事件。对早、中侏罗世植物群和沉积特征的综合分析表明,中国在早侏罗世晚期可划分出黑龙江东部温凉气候区、北方暖温带温暖潮湿气候区、中部热带—亚热带半干旱—半潮湿气候区、中南热带—亚热带干旱气候区和西藏—滇西热带干旱气候区共5个气候区,其中北方暖温带温暖潮湿气候区与中部热带—亚热带半干旱—半潮湿气候区的界线较早侏罗世早期和中侏罗世早期偏北4°～8°(纬度),亦说明早侏罗世晚期发生了升温事件。此升温事件与中亚和西伯利亚的图阿尔期升温事件相当,其很可能是早侏罗世晚期图阿尔期大洋缺氧事件(T-OAE)在陆地生态系统中的响应。     

我国干旱区洪水灾害基本特征：以新疆为例

依据新疆自然和社会环境,分析了新疆洪水及其洪灾的形成,季节分布,年际变化,地区分布等主要特征,并提出对洪水灾害的防御对策。     

基于Cloudsat的降水云和非降水云垂直特征

降水云是人工增雨作业的主要对象,了解降水云系的垂直结构对于人工增雨可播条件的选择至关重要。利用Cloudsat卫星2008年3月—2009年2月资料,首先通过大量个例分析并结合地面降水量观测验证Cloudsat卫星识别降水云方法的合理性,在此基础上,统计分析了华北和江淮地区降水云与非降水云的垂直结构特征。统计结果表明:降水云与非降水云垂直结构存在明显差异, 两地区降水云云底高度都在2 km以下,非降水云的云底高度以高于2 km为主。两地区单层降水云云厚以大于6 km为主,多层降水云云厚以2~4 km为主,非降水云云厚以小于2 km为主。两地区降水云夹层厚度集中于1~2 km,非降水云夹层厚度集中在4 km以上。江淮地区多层云降水频率略高于华北地区。