Can pharmaceuticals interfere with the synthesis of active androgens in male fish? An in vitro study

The in vitro interference of fibrate (gemfibrozil, clofibrate, clofibric acid), anti-inflammatory (ibuprofen, diclofenac), and anti-depressive (fluoxetine, fluvoxamine) drugs with key enzymatic activities—C17,20-lyase and CYP11β-involved in the synthesis of active androgens in gonads of male carp have been investigated. Among the tested compounds, fluvoxamine and fluoxetine were the strongest inhibitors of C17,20-lyase and CYP11β enzymes, with IC50s in the range of 321-335 μM and 244-550 μM, respectively. To our knowledge this is the first report on the interaction of pharmaceutical compounds with enzymatic systems involved in the synthesis of oxy-androgens. As oxy-androgens are known to influence spermatogenesis and stimulate reproductive behavior and secondary sexual characteristics in male fish, this work highlights the need for further investigating these endpoints when designing specific in vivo studies to assess the endocrine disruptive effect of pharmaceuticals in fish.     

Magmatic evolution of a dying spreading axis: Evidence for the interaction of tectonics and mantle heterogeneity from the fossil Phoenix Ridge,Drake Passage

New ⁴⁰Ar–³⁹Ar ages of 5.6 to 1.3 Ma for lavas from the fossil Phoenix Ridge in the Drake Passage show that magmatism continued for at least 2 Ma after the cessation of spreading at 3.3 ± 0.2 Ma. The Phoenix Ridge lavas are incompatible element-enriched relative to average MORB and show an increasing enrichment with decreasing age, corresponding to progressively decreasing degrees of partial melting of spinel peridotite after spreading stopped. The low-degree partial melts increasingly tap a mantle source with radiogenic Sr and Pb but unradiogenic Nd isotope ratios implying an ancient enrichment. The post-spreading magmas apparently form by buoyant ascent of enriched and easily fusible portions of the upper mantle. Only segments of fossil spreading ridges underlain by such enriched and fertile mantle show post-spreading volcanism frequently forming bathymetric highs. The Phoenix Ridge lavas belong to the Pacific, rather than the Atlantic, mantle domain in regional Sr–Nd–Pb space. Our new data show that the southern Pacific Ocean mantle is heterogeneous containing significant enriched portions that are preferentially tapped at low melt fractions. Isotopic mapping reveals that Pacific-type upper mantle flows eastward through Drake Passage and surrounds the subducting Phoenix Plate beneath the Bransfield Basin.     

Petroleum degradation and associated microbial signatures at the Chapopote asphalt volcano, Southern Gulf of Mexico

At the Chapopote Knoll in the Southern Gulf of Mexico, deposits of asphalt provide the substrate for a prolific cold seep ecosystem extensively colonized by chemosynthetic communities. This study investigates microbial life and associated biological processes within the asphalts and surrounding oil-impregnated sediments by analysis of intact polar membrane lipids (IPLs), petroleum hydrocarbons and stable carbon isotopic compositions (δ¹³C) of hydrocarbon gases. Asphalt samples are lightly to heavily biodegraded suggesting that petroleum-derived hydrocarbons serve as substrates for the chemosynthetic communities. Accordingly, detection of bacterial diester and diether phospholipids in asphalt samples containing finely dispersed gas hydrate suggests the presence of hydrocarbon-degrading bacteria. Biological methanogenesis contributes a substantial fraction to the methane captured as hydrate in the shallow asphalt deposits evidenced by significant depletion in ¹³C relative to background thermogenic methane. In sediments, petroleum migrating from the subsurface stimulates both methanogenesis and methanotrophy at a sulfate-methane transition zone 6-7 m below the seafloor. In this zone, microbial IPLs are dominated by archaeal phosphohydroxyarchaeols and archaeal diglycosidic diethers and tetraethers. Bacterial IPLs dominate surface sediments that are impregnated by severely biodegraded oil. In the sulfate-reduction zone, diagnostic IPLs indicate that sulfate-reducing bacteria (SRB) play an important role in petroleum degradation. A diverse mixture of phosphohydroxyarchaeols and mixed phospho- and diglycosidic archaeal tetraethers in shallow oil-impregnated sediments point to the presence of anaerobic methane-oxidizing ANME-2 and ANME-1 archaea, respectively, or methanogens. Archaeal IPLs increase in relative abundance with increasing sediment depth and decreasing sulfate concentrations, accompanied by a shift of archaeol-based to tetraether-based archaeal IPLs. The latter shift is suggested to be indicative of a community shift from ANME-2 and/or methanogenic archaea in shallower sediments to ANME-1/methanogenic archaea and possibly benthic archaea in deeper sediments.     

Species-dependent sensitivity to contaminants: an approach using primary hepatocyte cultures with three marine fish species

There is limited knowledge about the sensitivity of different fish species to environmental pollutants. Such information is pivotal in risk assessment and to understand why some species appear to be more tolerant to contaminants than others. The aim of the current study was to evaluate whether primary hepatocyte cultures of three marine fish species could be established in the field and whether their sensitivity to selected contaminants would differ. Primary hepatocyte cultures of three marine fish species (plaice, long rough dab, Atlantic cod) were established and exposed for 24 h to copper (20-2500 mg L?1) and statins (1-200 mg L?1). Endpoints were esterase activity, metabolic activity and reduced glutathione (GSH) content, all using fluorescent probes. Flatfish hepatocytes were more susceptible to copper and statin exposure than hepatocytes from cod. This study has shown that species-dependent differences in contaminant sensitivity can be investigated using primary hepatocyte cultures.     

Catchments as reactors: a comprehensive approach for water fluxes and solute turnover

Sustainable water quality management requires a profound understanding of water fluxes (precipitation, run-off, recharge, etc.) and solute turnover such as retention, reaction, transformation, etc. at the catchment or landscape scale. The Water and Earth System Science competence cluster (WESS, http://www.wess.info/) aims at a holistic analysis of the water cycle coupled to reactive solute transport, including soil–plant–atmosphere and groundwater–surface water interactions. To facilitate exploring the impact of land-use and climate changes on water cycling and water quality, special emphasis is placed on feedbacks between the atmosphere, the land surface, and the subsurface. A major challenge lies in bridging the scales in monitoring and modeling of surface/subsurface versus atmospheric processes. The field work follows the approach of contrasting catchments, i.e. neighboring watersheds with different land use or similar watersheds with different climate. This paper introduces the featured catchments and explains methodologies of WESS by selected examples.     

Effect of Pumice and Sand on the Sustainability of Granular Iron Beds for the Aqueous Removal of CuII,NiII, and ZnII

Current knowledge of the basic principles underlying the design of Fe⁰ beds is weak. The volumetric expansive nature of iron corrosion was identified as the major factor determining the sustainability of Fe⁰ beds. This work attempts to systematically verify developed concepts. Pumice and sand were admixed to 200 g of Fe⁰ in column studies (50:50 volumetric proportion). Reference systems containing 100% of each material have been also investigated. The mean grain size of the used materials (in mm) were 0.28 (sand), 0.30 (pumice), and 0.50 (Fe⁰). The five studied systems were characterized (i) by the time dependent evolution of their hydraulic conductivity (permeability) and (ii) for their efficiency for aqueous removal of Cu^II, Ni^II, and Zn^II (about 0.3 mM of each). Results showed unequivocally that (i) quantitative contaminant removal was coupled to the presence of Fe⁰, (ii) additive admixture lengthened the service life of Fe⁰ beds, and (iii) pumice was the best admixing agent for sustaining permeability while the Fe⁰/sand column was the most efficient for contaminant removal. The evolution of the permeability was well‐fitted by the approach that the inflowing solution contained dissolved O₂. The achieved results are regarded as starting point for a systematic research to optimize/support Fe⁰ filter design.     

The influence of housing oversupply on residential segregation: exploring the post-socialist city of Leipzig†

In this article, we contribute to a better understanding of contextual differences related to residential segregation. We illuminate one specific contextual factor—housing oversupply—and how it intersects with historically inherited patterns of socio-spatial differentiation and other drivers of residential segregation. The study is based on an analysis of how segregation has developed over the last 20 years in the city of Leipzig, Germany. This case offers the rare possibility of studying the impact of city-wide housing oversupply on residential segregation, rather than concentrating on decline or decay in specific areas. We examine how oversupply emerged at the meeting point of changes in market structures, housing preferences, welfare state interventions, and migration trends in the post-socialist transition. Using existing statistical data, we demonstrate how oversupply has fostered a fast and thorough reshuffling of residential patterns. After a period of resolving segregation patterns from the socialist era, oversupply acts as a catalyst for recently emerging residential segregation patterns.     

Joint estimation of GPS/BDS real-time clocks and initial results

We present the joint estimation model for Global Positioning System/BeiDou Navigation Satellite System (GPS/BDS) real-time clocks and present the initial satellite clock solutions determined from 106 stations of the international GNSS service multi-GNSS experiment and the BeiDou experimental tracking stations networks for 1 month in December, 2012. The model is shown to be efficient enough to have no practical computational limit for producing 1-Hz clock updates for real-time applications. The estimated clocks were assessed through the comparison with final clock products and the analysis of post-fit residuals. Using the estimated clocks and corresponding orbit products (GPS ultra-rapid-predicted and BDS final orbits), the root-mean-square (RMS) values of coordinate differences from ground truth values are around 1 and 2–3 cm for GPS-only and BDS-only daily mean static precise point positioning (PPP) solutions, respectively. Accuracy of GPS/BDS combined static PPP solutions falls in between that of GPS-only and BDS-only PPP results, with RMS values approximately 1–2 cm in all three components. For static sites, processed in the kinematic PPP mode, the daily RMS values are normally within 4 and 6 cm after convergence for GPS-only and BDS-only results, respectively. In contrast, the combined GPS/BDS kinematic PPP solutions show higher accuracy and shorter convergence time. Additionally, the BDS-only kinematic PPP solutions using clock products derived from the proposed joint estimation model were superior compared to those computed using the single-system estimation model.     

SARAL/AltiKa Global Statistical Assessment and Cross-Calibration with Jason-2

The CNES/ISRO mission SARAL/AltiKa was successfully launched on 25 February 2013. It reached its nominal orbit on 13 March 2013. AltiKa is the first altimeter using the Ka-band frequency. This article presents the results of the calibration and validation activities perfromed on the first year of the SARAL/AltiKa mission. The main objective of the article is to assess the SARAL/AltiKa data quality and to estimate the altimeter system performance using GDR products. To achieve this goal, we present mono-mission metrics and compare them with Jason-2 over the same period. Even if these missions do not have the same ground track, precise comparisons are still possible. They allow assessing parameter discrepancies and SSH consistency between both missions in order to detect geographically correlated biases, jumps or drifts. These results show that SARAL/AltiKa data quality is excellent: ocean data coverage is greater than 99.5%, standard deviation at cross-overs is 5.4 cm. The mission therefore fulfills the requirements of high precision altimetry and can be used (in conjunction with Jason-2) to monitor the global mean sea level, ensuring the continuity of the record over ERS/Envisat historical ground track. Possible improvements and open issues are also identified, foreseeing an even better mission performance.     

How to Find Aquifer Statistics Utilizing Pumping Tests? Two Field Studies Using welltestpy

We present a workflow to estimate geostatistical aquifer parameters from pumping test data using the Python package welltestpy . The procedure of pumping test analysis is exemplified for two data sets from the Horkheimer Insel site and from the Lauswiesen site, Germany. The analysis is based on a semi-analytical drawdown solution from the upscaling approach Radial Coarse Graining, which enables to infer log-transmissivity variance and horizontal correlation length, beside mean transmissivity, and storativity, from pumping test data. We estimate these parameters of aquifer heterogeneity from type-curve analysis and determine their sensitivity. This procedure, implemented in welltestpy , is a template for analyzing any pumping test. It goes beyond the possibilities of standard methods, for example, based on Theis' equation, which are limited to mean transmissivity and storativity. A sensitivity study showed the impact of observation well positions on the parameter estimation quality. The insights of this study help to optimize future test setups for geostatistical aquifer analysis and provides guidance for investigating pumping tests with regard to aquifer statistics using the open-source software package welltestpy .