6种鱼类肌肉组织肌苷酸的检测分析

采用高效液相色谱技术,在国内首次测定了条纹斑竹鲨(Chilocsyllium plagiosum)、大黄鱼(Pseudosci-aena crocea)、鳜(Siniperca chuatsi)、青石斑鱼(Epinephelus awoara)、牙鲆(Paralichthys olivaceus)和乌塘鳢(Bostrichthys sinensis)等6种鱼类新鲜和冻存肌肉组织中肌苷酸含量。结果表明:(1)不同鱼类肌肉组织中肌苷酸质量比不同,牙鲆新鲜肌肉组织中肌苷酸质量比最高,为4.214 mg/g,其余5种鱼类为2.365~3.634 mg/g。(2)10 d冻存试验中,经-70℃超低温冻存,6种鱼类冻存肌肉组织中肌苷酸质量比均高于新鲜肌肉组织,大黄鱼肌肉组织冻存1 d肌苷酸质量比达最大值,鳜肌肉组织冻存10 d肌苷酸质量比呈上升态势未出现最大值,其余5种鱼类肌肉组织肌苷酸质量比均在冻存第5天达到最大值。长时间的冻存将会降低食用鱼肉中肌苷酸质量比,导致鲜味下降,但经1周左右短时间的冻存后可明显提高鱼肉中肌苷酸质量比,增加鲜味,提高食用价值。     

川纹笛鲷消化道优势菌群PCR-DGGE指纹图谱比较分析

采用免培养的16S rDNA梯度凝胶电泳技术（DGGE）对集约化海水网箱养殖川纹笛鲷（Lutjanus sebae）的消化道胃壁、胃内容物、肠壁、肠内容物优势菌群结构进行了比较分析。研究结果显示,川纹笛鲷消化道存在着丰富多样的细菌群落,对DGGE指纹图谱聚类分析表明菌群组成相似度高于55％,其中胃内容物及胃壁细菌组成相似度最高（90％）,这可能与摄食饵料在消化道推移有关;而胃壁与肠壁相似度相对最差,可能反应了由于生理环境不同引起的宿主差异性。通过建立川纹笛鲷消化道16S rDNA-DGGE指纹图谱及比较分析,为澄清川纹笛鲷消化道微生物区系奠定了基础。     

大黄鱼头肾免疫细胞研究

对大黄鱼（Pseudosciaena,crocea）头肾印迹片、光镜片和电镜片进行观察。结果表明,大黄鱼头肾包括淋巴细胞聚集区和颗粒细胞聚集区,但无肾小球结构。头肾免疫细胞丰富,包括淋巴细胞、颗粒细胞（Ⅰ型和Ⅱ型）、单核细胞和巨噬细胞,分别占57．94％、36．43％、5．63％,表明头肾是大黄鱼免疫细胞的生发中心,在免疫防御系统中具重要作用。     

尖刀蛏生化成分和营养价值评价

分析了尖刀蛏(Cultelles scalprum)肉的生化成分,并对其营养价值进行评定.结果显示:尖刀蛏的含肉率为60.3%,鲜肉中的水分、蛋白质、脂肪和灰分含量分别为84.21%、10.85%、1.31%和1.71%,人体必需氨基酸占氨基酸总量的37.3%,不饱和脂肪酸含量占总脂肪的43.1%,其中廿碳五烯酸(C20:5,EPA)和廿二碳六烯酸(C22:6,DHA)的含量分别占总脂肪的7.8%和15.7%.研究结果表明,尖刀蛏含肉率高,营养丰富,有较高的营养价值和保健价值.     

单舰航线水文气象保障特点和思考

文中总结单舰航线水文气象保障业务的特点、难点,并结合航线水文气象保障的现状提出一些个人的建设性建议。     

基于Barnes滤波原理的降水场客观分析及尺度分离

基于Barnes滤波原理（又称高斯加权客观分析）,在进行网格点插值的同时,通过选择适当的滤波参数C、G滤去原始场中的短波噪音,使分析结果平稳光滑;另外可通过它构成一带通滤波器,根据实际需要分离出影响天气过程的各种次天气尺度,达到尺度分离的目的。以全国160个国家基准代表站1954—2006年共53a的年平均降水场为例作实例分析,并同用Grads内插函数（Oacres函数）、九点平滑函数（Smth9函数）的绘图结果进行对比分析,结果表明客观分析结果同Grads内插平滑分析结果基本相似,都能反映出我国53a年平均降水场从东南向西北逐渐递减的趋势且与实际情况相符,降水场高值中心位于长江流域及以南的华南等地区,低值中心位于黄河流域以北的广大西部地区。取不同的参数C和G得到的尺度分离后的带通滤波值,既抑止了长波,又抑制了短波,达到较好的尺度分离效果。     

巢湖地学实习基地数字化平台的建立

利用GIS工具（MapInfo）对地图（例如巢湖地学基地）进行矢量化,打下建立数字化平台的基础。再根据断层,等高线等分布资料,利用GIS的空间分析功能描绘出整个巢湖地学基地地形分布图。对地形分布图加入它的属性,从而可以比较直观系统地对其分析．也方便了地学工作者。     

Spatial Distribution Assessment and Source Apportionment of Land-Based Pollutant Yield from Qingdao,China

The spatial distribution of chemical oxygen demand(COD) and total nitrogen(TN) yield from Qingdao are studied by comparing pollutant yield amount, densities and spatial aggregation(Getis-Ord indexes) among the land-based pollutant source regions(PSRs) entering the three sub-seas(i.e. the Jiaozhou Bay(JZB), other coastal area in the Yellow Sea(OCAYS) and Laizhou Bay(LZB), respectively). Industrial composition of the loads are also studied by comparing pollutant yield among the sources of agriculture, rural domesticity, industry, urban domesticity and service, and calculation of Gini coefficient. Results show that spatial distribution of COD and TN yield from Qingdao are extremely unbalanced. The JZB, with less than 3% of the total coastal sea area of Qingdao, received 62% COD load and 65% TN yield from Qingdao, while the OCAYS, with more than 97% area, only received 23% COD and 20% TN, which consist with the much worsen water quality of JZB than that of OCAYS. On the other hand, the source apportionment of COD and TN loads in the PSRs entering JZB and the OCAYS was similar. The agricultural and domestic sources with high pollution intensity account for more than 80%, while the industrial and service sources with low pollution intensity account for less than 20%. While Gini coefficients, COD 0.81 and TN 0.84 which are much higher than the ‘imbalance' threshold of 0.4, show the uneven industrial structure of Qingdao. These results may be useful in the determination of land-based pollution total amount control at the PSR level.     

Estimating the maximum impact force of dry granular flow based on pileup characteristics

The maximum normal impact resultant force(NIRF)is usually regarded as the sum of the static earth pressure of the dead zone and the dynamic impact pressure of the flowing layer.The influence of the interaction between the flowing layer and dead zone on the impact force is ignored.In this study,we classified two impact models with respect to the pileup characteristics of the dead zone.Then,we employed the discrete element method to investigate the influences of the pileup characteristics on the impact force of dry granular flow on a tilted rigid wall.If the final pileup height is equal to the critical value,the maximum NIRF can be estimated using a hydrostatic model,because the main contribution to the maximum NIRF is the static earth pressure of the dead zone.If the final pileup height is less than the critical value,however,the particles in the dead zone are squeezed along the slope surface by the impact ofthe flowing layer on the dead zone,and because of shear effects,the flowing layer causes an entrainment in the dead zone.This results in a decrease in the volume of the dead zone at the moment of maximum NIRF with increases in the slope angle.As such,the maximum NIRF mainly comprises the instant impact force of the flowing layer,so hydro-dynamic models are effective for estimating the maximum NIRF.Impact models will benefit from further study of the components and distribution of the impact force of dry granular flow.     

深水等深流沉积中记录的米兰科维奇特性——以陕西富平地区上奥陶统赵老峪组为例

陕西富平地区上奥陶统赵老峪组为深水盆地边缘至深水斜坡沉积环境,发育深水原地沉积、等深流沉积及重力流沉积。以野外实测剖面、岩相特征等资料为基础,结合米兰科维奇理论、天文地层学、古海洋学以及古气候学,系统研究该地区等深流强度与古气候、海平面、古盐度的关系。结果表明:Fischer图解可以客观、准确地反映深水沉积地层相对海平面变化;深水沉积及等深流沉积中不同级次旋回垂向组合关系记录了米兰科维奇特性,即小级别旋回在垂向上有序地按照1∶4或者1∶5的形式叠加构成较大级别旋回;等深流强度受米兰科维奇旋回影响,呈多周期弱→强→弱递变;等深流强化周期约为0.1Ma。