Inter-relationships of MnO2 precipitation, siderophore-Mn complex formation, siderophore degradation, and iron limitation in Mn-oxidizing bacterial cultures

To examine the pathways that form Mn^(III) and Mn^(IV) in the Mn^(II)-oxidizing bacterial strains Pseudomonas putida GB-1 and MnB1, and to test whether the siderophore pyoverdine (PVD) inhibits Mn^(IV)O₂ formation, cultures were subjected to various protocols at known concentrations of iron and PVD. Depending on growth conditions, P. putida produced one of two oxidized Mn species - either soluble PVD-Mn^(III) complex or insoluble Mn^(IV)O₂ minerals - but not both simultaneously. PVD-Mn^(III) was present, and MnO₂ precipitation was inhibited, both in iron-limited cultures that had synthesized 26-50 μM PVD and in iron-replete (non-PVD-producing) cultures that were supplemented with 10-550 μM purified PVD. PVD-Mn^(III) arose by predominantly ligand-mediated air oxidation of Mn^(II) in the presence of PVD, based on the following evidence: (a) yields and rates of this reaction were similar in sterile media and in cultures, and (b) GB-1 mutants deficient in enzymatic Mn oxidation produced PVD-Mn^(III) as efficiently as wild type. Only wild type, however, could degrade PVD-Mn^(III), a process linked to the production of both MnO₂ and an altered PVD with absorbance and fluorescence spectra markedly different from those of either PVD or PVD-Mn^(III). Two conditions, the presence of bioavailable iron and the absence of PVD at concentrations exceeding those of Mn, both had to be satisfied for MnO₂ to appear. These results suggest that P. putida cultures produce soluble Mn^(III) or MnO₂ by different and mutually inhibitory pathways: enzymatic catalysis yielding MnO₂ under iron sufficiency or PVD-promoted oxidation yielding PVD-Mn^(III) under iron limitation. Since PVD-producing Pseudomonas species are environmentally prevalent Mn oxidizers, these data predict influences of iron (via PVD-Mn^(III) versus MnO₂) on the global oxidation/reduction cycling of various pollutants, recalcitrant organic matter, and elements such as C, S, N, Cr, U, and Mn.     

Impact of storm runoff from tropical watersheds on coastal water quality and productivity

Storm runoff in the steep watersheds in Hawaii leads to sediment and freshwater pulses to coastal waters that quickly affect nearshore water quality. This is particularly true in semi-enclosed embayments, such as Kaneohe Bay, Oahu, where water has a relatively long residence time compared to more open coastal areas of the islands. In this paper the authors discuss water quality and productivity in Kaneohe Bay after back-to-back rain events in late November and early December 2003, following a particularly dry summer. The short-term biogeochemical response of coastal waters and the ecosystem to runoff and physical forcing was evaluated through a combination of continuous in situ measurements and adaptive synoptic sampling carried out on a variety of temporal and spatial scales.     

Multiple phases of North African humidity recorded in lacustrine sediments from the Fazzan Basin, Libyan Sahara

The Fazzan Basin of south-west Libya is at present arid with less than 20 mm of rainfall per annum. However, regionally extensive limestones, lacustrine sands and coquina (fossiliferous carbonate rock) deposits show that the Fazzan Basin previously contained a large palaeolake, indicating that the climate in the past was more humid. Optically stimulated luminescence (OSL) dating techniques have been applied to key lacustrine deposits within the basin in an attempt to provide an internally consistent chronology for this humidity record. Results indicate that palaeolake sediments within the Fazzan Basin record a very long history of palaeohydrological change, ranging from present day arid conditions to humidity capable of sustaining a lake with an approximate area of 76,250 km². The existence of humid periods in mid oxygen isotope stage 5 and the early Holocene is confirmed. An older lacustrine event, tentatively correlated to oxygen isotope stage 11, is also recognized. In addition, evidence is presented for at least two humid phases beyond the age range over which the conventional OSL dating technique is applicable. This study demonstrates that OSL dating of palaeolake sediments within the Fazzan Basin offers the potential to provide a detailed record of North African humidity spanning several glacial–interglacial cycles.     

Initial CHIP He I Observations of Solar Limb Activity

A new instrument capable of 3-min time resolution full-disk and limb observations in the Hei 1083 nm spectral line has been in operation at the High Altitude Observatory's Mauna Loa Solar Observatory (MLSO) since April 1996. We discuss instrument capabilities and performance and present some initial observations of limb activity from the first year of instrument operation. We compare limb Hei and Hα observations of quiescent and active prominences, comment on the role of Doppler shifts in interpreting the Hei observations, and illustrate the use of disk/limb Hei observations of a CME-associated eruptive filament in mass-ejection studies.     

Images of Gradual Millimeter Emission and Multi-Wavelength Observations of the 17 august 1994 Solar Flare

We present a comprehensive analysis of the 17 August 1994 flare, the first flare imaged at millimeter (86 GHz) wavelengths. The temporal evolution of this flare displays a prominent impulsive peak shortly after 01:02 UT, observed in hard X-rays and at microwave frequencies, followed by a gradual decay phase. The gradual phase was also detected at 86 GHz. Soft X-ray images show a compact emitting region (20), which is resolved into two sources: a footpoint and a loop top source. Nonthermal emissions at microwave and hard X-ray wavelengths are analyzed and the accelerated electron spectrum is calculated. This energy spectrum derived from the microwave and hard X-ray observations suggests that these emissions were created by the same electron population. The millimeter emission during the gradual phase is thermal bremsstrahlung originating mostly from the top of the flaring loop. The soft X-rays and the millimeter flux density from the footpoint source are only consistent with the presence of a multi-temperature plasma at the footpoint.     

Reconciling feminist theory and gendered resource management in Indonesia

Summary This paper considers the difficulties in reconciling a fluid and ambiguous conceptualization of gender difference with policy-based efforts to define women as 'target groups' in development interventions. The issue is explored with reference to gender issues in a transmigration resettlement area in Indonesia, where the instability of gender identities is particularly marked, and where gendered resource use and control are particularly blurred.     

Visualization of the El Berrocal granite: application to rock engineering

This paper outlines the visualization of the El Berrocal granite using a computer-based geological modelling system, EarthVision, and discusses the application of this visualization to engineering aspects of waste disposal in crystalline rocks. The El Berrocal Project was an international study with the aim of understanding and modelling the migration processes which have controlled the distribution of naturally occurring radionuclides in a fractured granitic environment. EarthVision was used to provide three-dimensional geological models of the site structure and properties. Modelling of the site structure concentrated on the development of visualizations of the main discontinuities in the granite. These included a model of the main mineralized structures, a model of the regional fracture network, models of local fracture networks between borehole clusters and a visualization of the mineralogy of the fractures in individual boreholes. These fracture models were visualized with the boreholes and access gallery to the mine. In addition, the fracture network in the region of a large scale tracer test was visualized with the injection and extraction zones for the tracer test. Three-dimensional interpolations of the rock and fluid structure were undertaken. A model of the hydraulic conductivity illustrated large-scale variations in hydraulic conductivity and channelling effects in the tracer test zone. A model of the sulphate concentrations in the groundwater illustrated the interpolation of spatial data based on structural domains. The visualizations of the geology of the El Berrocal granite illustrate that, despite limitations, geological modelling can be a powerful and graphic tool in rock engineering. The use of computer visualizations can be provide the three-dimensional structural framework for computations, can aid decision making during the construction phase of waste repositories and can be useful in understanding and analysing the results of numerical calculations.     

Estimates for the luminosity function of galaxies and its evolution

A new method is presented to obtain a non-parametric maximum likelihood estimate of the luminosity function and the selection function of a flux-limited redshift survey. The method parametrizes the selection function as a series of stepwise power laws and allows possible evolution of the luminosity function. We also propose a new technique to estimate the rate of evolution of the luminosity function. This is based on a minimization of the observed large-scale power with respect to the evolutionary model. We use an ensemble of mock surveys extracted from an N -body simulation to verify the power of this method. We apply our estimators to the 1.2-Jy survey of IRAS galaxies. We find a far-infrared luminosity function in good agreement with previously published results and evidence for rather strong evolution. If the comoving number density of IRAS galaxies is assumed to scale ∝ (1 +  z ) ^P , we estimate P  = 4.3 ± 1.4.     

Tectonic deformation and recrystallisation of oligoclase

Naturally deformed and partially recrystallised oligoclase porphyroclasts were studied in a high voltage electron microscope. The oligoclases had a highly deformed outer mantle zone, which contained high densities of dislocations and albite and pericline deformation twins, and less deformed cores containing fewer dislocations and albite twins but no pericline deformation twins. There was little evidence for recovery; apparently the internal strain energy due to deformation was relieved by recrystallisation. Strain free nuclei developed in areas with the highest defect densities. The resultant new grains had a lower anorthite content than their parents. It is suggested that the recrystallisation processes were aided by strain enhanced diffusion.