The pathogenic species of genus Vibrio cause vibriosis, one of the most prevalent diseases of maricultured animals and seafood consumers. Monitoring their kinetics in the chain of seafood production, processing and consumption is of great importance for food and mariculture safety. In order to enrich Vibrio-representing 16S ribosomal RNA gene (rDNA) fragments and identify these bacteria further real-timely and synchronously among bacterial flora in the chain, a pair of primers that selectively amplify Vibrio 16S rDNA fragments were designed with their specificities and coverage testified in the analysis of seawater Vibrio community. The specificities and coverage of two primers, VF169 and VR744, were determined theoretically among bacterial 16S rDNAs available in GenBank by using BLAST program and practically by amplifying Vibrio 16S rDNA fragments from seawater DNA. More than 88.3% of sequences in GenBank, which showed identical matches with VR744, belong to Vibrio genus. A total of 33 clones were randomly selected and sequenced. All of the sequences showed their highest similarities to and clustered around those of diverse known Vibrio species. The primers designed are capable of retrieving a wide range of Vibrio 16S rDNA fragments specifically among bacterial flora in seawater, the most important natural environment of seafood cultivation. 相似文献
Binary neutron star(NS)mergers may result in remnants of supra-massive or even stable NS,which have been supported indirectly by observed X-ray plateau of some gamma-ray burst(GRB)afterglows.Recently,Xue et al.(2019)discovered an X-ray transient CDF-S XT2 that is powered by a magnetar from merger of double NS via X-ray plateau and following stepper phase.However,the decay slope after the plateau emission is slightly larger than the theoretical value of spin-down in electromagnetic(EM)dominated by losing its rotation energy.In this paper,we assume that the feature of X-ray emission is caused by a supra-massive magnetar central engine for surviving thousands of seconds to collapse into a black hole.Within this scenario,we present the comparisons of the X-ray plateau luminosity,break time,and the parameters of magnetar between CDF-S XT2 and other short GRBs with internal plateau samples.By adopting the collapse time to constrain the equation of state(EOS),we find that three EOSs(GM1,DD2,and DDME2)are consistent with the observational data.On the other hand,if the most released rotation energy of magnetar is dominated by GW radiation,we also constrain the upper limit of ellipticity of NS for given EOS,and its range is[0.32-1.3]×10-3.Its GW signal cannot be detected by Advanced LIGO or even for more sensitive Einstein Telescope in the future. 相似文献