Geochemical evidence of paleogeography and paleoclimate during deposition of the 2nd Member of Kongdian Formation in Kongnan area

The 2nd Member of Kongdian Formation has been made up of a large number of oil shale and mudstone in the Kongnan aera of Huanghua depression around the Bohai Bay. In the Kongnan area, the lake basins were very large and deep during the deposition of the 2nd Member of Kongdian Formation. During that period,the lakes were sealed, uncommunieated with the sea water and the paleoclimate was very warm and wet in Kongnan area. Analyzing the content of the trace element and the rare earth element, carbon and oxygen isotope in the disquisition, The authors prove the two views correct.     

莒南县努力搞好土地开发整理工作

莒南县地处鲁东南地区,东临日照市,西靠临沂市河东区,北接日照市莒县,南连临沭县,东南与江苏省交界.辖18个乡镇,937个行政村,99万人口,土地总面积1 755 km^2,耕地面积7.27万hm^2,列入国家、省、市土地开发整理的后备资源规划面积2.67万hm^2（40万亩）,土地后备资源开发潜力较大.     

一株H_5N_1亚型禽流感病毒株NA基因的克隆与序列分析

用RT-PCR方法从1个H5N1亚型禽流感病毒分离株A/Chicken/Guangdong/DH/1997扩增NA基因cDNA片段,将其克隆至pMD18-T载体,获得重组质粒pMD-NA,并对其核苷酸序列进行测定和分析。结果表明,该毒株的NA基因长度为1350bp,编码449个氨基酸,与其它H5N1亚型AIV分离株的核苷酸序列同源性为97.0%～99.4%,氨基酸序列同源性为97.7%～99.1%,提示禽流感病毒NA基因保守性较高。NA基因氨基酸序列的聚类分析表明该毒株与来自香港的A/Pheasant/HK/FY155/01和A/Ch/HK/FY150/01两个分离株处于同一进化枝,亲缘关系较近。     

鲤JAK2基因分子克隆及组织表达分析

采用同源克隆策略和RACE-PCR技术,克隆得到可能的鲤(Cyprinus carpio)两面神激酶2(JAK2)基因的c DNA全长序列,包括3 378 bp的开放阅读框,732 bp的5'-非编码区,529 bp的3'-非编码区,总长度达4 639bp。开放阅读框可编码1 125个氨基酸,推测分子质量和理论等电点分别为129.33 ku和6.99。同源性分析显示,克隆的鲤鱼基因与斑马鱼(Danio rerio)JAK2同源性最高,其氨基酸序列同一性和相似度分别达89%和95%。结构域预测表明,所编码氨基酸序列包含FERM、SH2及两个酪氨酸激酶结构域,这4个结构域也保守地存在于其他脊椎动物的JAK分子中。高级结构预测显示,鲤JAK2主要包括α-螺旋(α-helix)、β-折叠(β-sheet)和连接环(loop)等3类结构元件。脊椎动物JAK分子系统进化树显示,JAK1、JAK2、JAK3和TYK2等4类JAK分子分别聚类,鲤JAK2处于JAK2支系中,与所有其他的鱼类JAK2聚为一大支,并与两栖类和哺乳类组成的另一大支构成姊妹群,表明它们具有共同的祖先基因,为直系同源关系。实时荧光定量PCR检测结果表明,鲤JAK2在皮肤中表达量最高,其次是肠、血液和脑,而在肌肉、鳃、头肾、心、肝、脾中表达量较低;鲤JAK3在脾中表达量最高,在其他组织中表达量均很低,甚至未检出。     

Ingestion of <Emphasis Type="Italic">Brachionus plicatilis</Emphasis> under different microalgae conditions

The effects of four microalgae,Chlorella vulgaris,Platymonas helgolandicavar,Isochrysis galbana,and Nitzschia closterium on the grazing and filtering rates of the marine rotifer,Brachionus plicatilis,were evaluated under laboratory conditions.The grazing rates in separate cultures of the four microalga were as follows:C.vulgaris > P.helgolandicavar > I.galbana > N.closterium.However,the filtering rates occurred in the following order:P.helgolandicavar > N.closterium > C.vulgaris > I.galbana.A mixed diets ex...     

长江三峡库区地震地形变监测研究

三峡地壳形变监测网络结合空间大地测量(GPS、InSAR)和传统大地测量的优点，在1998年以来的三峡工程建设期间和2003年水库首次蓄水中获取了大量监测成果。结果显示库区地壳运动的基本图像：垂直形变较为显著，主要来自因蓄水荷载而产生的形变，水平形变不明显．库区主要断层的活动性在蓄水期间有明显加强迹象。水准测量反映从1998年首期观测至2003年10月首次蓄水后的库区垂直位移．沉降范围较大；GPS给出了2003年蓄水前后库区的垂直位移，形变的范围主要集中在水库近岸区域。水准测量与GPS观测反应的垂直位移的量级基本一致，沉降的最大幅度在3～4cm左右。水准监测、GPS观测与重力监测结果有一定的对应性。从地壳形变监测与研究结果分析，近期负荷改变而诱发中强地震的可能性不大。     

Characteristics and Hydrocarbon Potential of Mesozoic Strata in Eastern Pearl River Mouth Basin, Northern South China Sea

The well LF35-1-1 in the eastern Pearl River Mouth basin (PRMB) of the northern South China Sea revealed nnmetamorphosed Middle-Late Jurassic neritic-bathyal sediments and Cretaceous fluvial-lacustrine sediments.Three tectonic movements were identified in Late Jurassic to Early Cretaceous,late Early Cretaceous,and Late Cretaceous to Paleocene,respectively.The Late Jurassic marine facies mainly contain the hydrocarbon source and reservoir-seal assemblages,providing a main exploration target.     

1988年澜沧、耿马大震的预报和再认识

本文介绍了作者在1988年11月6日澜沧、耿马大震前进行中、短临阶段预报及大震现场预报的主要思路、方法和依据。中期预报的主要依据是:地震活动特征、大震的47年重现周期和应变能的积累;短临预报的主要依据是:滇西南地震活动中心的动态演变、M_L≥5.0级地震震中定向迁移及地震活动过程。大震后现场跟踪预报和最大强余震震级判别主要用了∑√E—lgt和b值截距法。最后本文对预报情况进行了总结和重新认识。     

Analysis of the spectrum of the coronal condensation of the total eclipse of 1983 June 11

We analyzed the spectrum of a coronal condensation observed during the total eclipse of 1983 June 11. Under the assumption of rotational symmetry we found the distributions of the electron density and the temperature. The electron density was higher in the centre of the condensation than on the edge. From different line pairs the maximum electron density was found to be between 1.7 3.5×10⁹cm⁻³ while the temperature was found to be basically uniform at 1.7 2.7×10⁶K. The turbulence velocity was 15 35km/s. The systematic flow of the condensation region with respect to the quiet corona was not more than 15km/s. Discussion of the instrumental profile showed that its effect was small on lines that were much wider than the profile.     

The comparison between allozyme and RAPD makers for the population genetic structure analysis of scallop Chlamys farreri

1INTRODUCTION Since the first use in human genetic research in1966,allozyme has become the most popular genetic marker for population genetics studies for almost all groups of animals and plants(Lewontin and Hubby,1966;Murphy et al.,1996).In the past seve…