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91.
92.
93.
Dolmatova L. S. Eliseykina M. G. Timchenko N. F. Kovaleva A. L. Shitkova O. A. 《中国海洋湖沼学报》2003,21(4):293-304
Pure fraction (92%–95%) of phagocytes (FP) and a mixture of amoebocytes (62%) and morula cells (38%)-FPMC- of the holothurianEupentacta fraudatrix' (Holothuroidea, Dendrochirota) were obtained by using ficoll-verographine step gradient. Basal production of reactive oxygen species (ROS) in FP quantified
by using reduction of nitroblue tetrazolium (NBT) was more than twice that in FPMC. Thermostable toxin ofYersinia pseudotuberculosis (TST) at different concentrations (0.2; 0.5; 2.5 μg/ml, but not 0.1 μg/ml) stimulated NBT reduction in FPMC after 24 h incubation.
In FP, TST at concentrations of 0.1 and 0.2 μg/ml inhibited and at concentrations of 0.5 and 2.5 μg/ml stimulated NBT reduction
after 24 h incubation. Maximal effect was observed in FP and FPMC at TST concentrations of 0.5 and 0.2 μg/ml, respectively.
Addition of catalase (0.7 μg/ml) to the cells treated with TST (2.5 μg/ml) was followed by a decrease in NBT reduction compared
to that under toxin treatment alone. TST stimulated superoxide dismutase activity in concentration-dependent manner (maximum
at 0.5 μg/ml concentration in FP) after 24 h treatment, and this stimulation was prevented by a commercial catalase. Plant
lectin concanavalin A stimulated NBT reduction more than 5-fold in FPMC compared to the control. With addition of TST, lectin
stimulated ROS to lesser extent than that with lectin alone. When catalase, TST, and lectin were added into the FPMC simultaneously,
ROS increase was similar to that under lectin treatment alone. On the whole, data obtained indicated that ROS generation in
holothurian coelomocytes especially occurs in both stimulated and not stimulated phagocytes, and that changes in ROS production
by these cells may be one of the mechanisms of antibacterial protection of holothurians.
This work was financially supported by the Russian Foundation for Fundamental Research Grant (No. 00-04-48949). 相似文献
94.
地倾斜台站数字化、模拟观测数据频率特性研究 总被引:2,自引:2,他引:0
分析了四川、云南的部分地倾斜数字化观测与模拟观测资料的特性,从频率范围,周期10天以上的FFT谱特性,固体潮波振幅,相位特征值等方面,论述了地倾斜数字化资料较模拟记录资料的信息组成变化,并对资料的分析与应用进行了初步探讨。 相似文献
95.
Qin Chengzhi 《中国地震研究》2004,18(2):178-187
The migration of strong earthquakes is an important research topic because the migration phenomena reflect partly the seismic mechanism and involve the prediction of tendency of seismic activity. Research on migration of strong earthquakes has mostly focused on finding the phenomena. Some attempts on getting regularity were comparatively subjective. This paper suggests that there should be indices of migration in earthquake dataset and the indexes should have statistical meaning if there is regularity in the migration of strong earthquakes. In this study, three derivative attributes of migration, i.e., migration orientation, migration distance and migration time interval, were statistically analyzed. Results in the North China region show that the migration of strong earthquakes has statistical meaning. There is a dominant migration orientation (W by S to E by N), a dominant distance (≤100km and on the confines of 300~700km), and a dominant time interval (≤1a and on the confines of 3~4a). The results also show that the migration will differ slightly with different magnitude range or earthquake activity phase. 相似文献
96.
1StrongtideandastronomicalconditionsPartial solar eclipse occurred 4 times in 1964, 1982 and 2000 respectively. Time interval is about 3 Saros periods (one Saros period is 18 years and 10.33~11.33 days). Total lunar eclipse occurred 2 times in 1964 and 2000 respectively and 3 times in 1982. However, there was no lunar eclipse in 1966, 1984 and 2002. It seems that they had similar astro-nomical conditions and the best was in 1982. The studies about the effect of tide on the global climate… 相似文献
97.
J. Rajchl J. Bočcek D. Očenáš J. Škvarka P. Zimnikoval H. Murayama K. Ohtsuka 《Earth, Moon, and Planets》1995,68(1-3):479-486
Some results following from two contemporary photographical programs (Slovakia and Japan) for persistent meteor train spectra are compared. It shows that even though both programs are not too different according to their lenses and films used, the spectral interval detected is very different. In this respect prism as a dispersion element is more favourable than the grating with blaze wavelength near 610 nm. However, some results previously assumed as typicai for presented persistent trains-e.g. the forbidden Herzberg O2 emission, the NO2 continuum and OH red emissions-seem to be substanciated especially due to higher and linear dispersion of those grating spectra. 相似文献
98.
Zhang Qing Wang Li-fan Hu Jing-yao P. A. Mazzali Wang Zhen-ru 《Chinese Astronomy and Astrophysics》1995,19(4):426-431
We present the modeling of the ultraviolet and optical spectra obtained simultaneously on 1993 April 15 with the HST and at Lick Observatory. A Monte Carlo code is employed in the modeling and a comparison is made between models reported by different groups. With an atmosphere similar to the Sun in chemical composition, the observed spectral lines are well reproduced by a power law density structure of index around 20 except the strong H and HeI λ5876 lines which have peculiar absorption profiles. The photospheric velocity is found to be 9500 km/s and the blackbody temperature of the spectrum is 7990 K. For H and HeI λ5876, we suggest a two-component density structure which has a smoother layer located immediately outside the steeply decreasing inner envelope. The power law indices are most probably 20 and 3, respectively, with the transition point at about 13 000 km/s. In addition, this outer smooth layer serves to flatten the far UV spectrum as observed. 相似文献
99.
Four bromophenols were isolated from the extract of marine red alga,Rhodomela confervoides by column chromatography and HPLC methods. By means of spectroscopic methods inclding IR, MS, 1D, and 2D-NMR techniques,
their structures were elucidated as (1) 3-bromo-4,5-dihydroxy benzoic acid methyl ester; (2) bis (2,3-dibromo-4,5-dihydroxybenzyl)
ether; (3) 3,4-dibromo-5-(methoxymethyl)-1,2-benzenediol and (4) 3-bromo-4,5-dihydroxy-benzaldehyde. Compound 1 was first
isolated from the algae in nature, and 1, 4 were found to have selective cytotoxic activities against KB, Bel 7402 and A549
cells, 2 showed powerful antibacterial activities againstStaphylococcus aurens andPseudomonas aeruginosa.
Supported by National “863” Program (No. 2004AA625030, 2001AA620503), Quingdao marine sciences project (No. 04-2-NN-26) and
Key Knowledge Innovative Project of the Chinese Academy of Sciences (KZCX3-SW-215). 相似文献
100.
用遗传算法解算病态方程 总被引:7,自引:1,他引:6
对应用遗传算法解决病态方程问题进行了探讨。利用拟合法而不是通过法方程求解参数,从而避免了法方程系数求逆,使病态方程的解答有了较好的结果。通过模拟计算并和其他方法进行比较,证明该方法是可行的和有效的。 相似文献