郑州市市区风环境模拟研究

城市风环境是城市微气候研究的一个重要方向,对分析城市热岛效应、空气流通等具有重要意义。本文以郑州市市区为例,使用1971—2018年气象观测数据、2018年建筑分布数据（OSM）和2016年资源三号卫星数据作为数据源,通过运用气象学和GIS技术结合的方法,探究潜在通风廊道,科学量化城市形态对风环境的影响。研究首先借助WindNinja软件,对城市背景风环境进行模拟分析,该计算方法提高了风道定位的精度。然后利用卫星遥感数据制作了数字高程模型（DSM）,结合OSM计算下垫面地表粗糙度。进一步借助ArcGIS软件,利用最小成本路径法（LCP）确定城市潜在通风廊道的位置。结果表明：① 郑州市近年来平均风速缓慢下降,平均每10年下降0.26 m/s;全年主导风向东北风进入城市后受城市形态影响在京广铁路线附近以西逐渐转为东北偏东风,其中在京广快速路以东风速较高,在京广快速路以西风速较低;② 金水区西部、中原区、二七区以及管城区的地表粗糙度较高,通风环境较差;金水区东部和惠济区的地表粗糙度较低,通风环境较好;③根据盛行风向模拟的潜在通风廊道,其共同特点是趋向于低粗糙度的地区。     

Microbiota and Shelf Life of Whole and Gutted Pacific Saury(Cololabis saira) During Refrigerated Storage

Pacific saury(Cololabis saira)is usually sold as whole fish in wholesale markets,or in its gutted form which is easy for consumers to cook in retail markets.In order to assess the effect of gutting on the shelf life of Pacific saury during refrigerated storage and reveal the microbial community,fish samples grouped into(I)whole fish and(II)gutted fish were analyzed periodically for sensory,biochemical and microbiological characteristics,and high-throughput sequencing technology was used to investigate the microbiota.Results showed that the sensory score for the gutted fish became unacceptable on day 8,while the whole fish score remained acceptable for 10 days.The total volatile basic nitrogen(TVB-N)value of the gutted fish reached 30 mg N(100 g)^-1 on day 6,while that of the whole samples surpassed 30 mg N(100 g)^-1 on day 10.The thiobarbituric acid reactive substances(TBARS)value of the gutted fish got close to 5.0 mg kg^-1 on day 10,while that of the whole samples surpassed 5.0 mg kg^-1 on day 14.The aerobic plate colony counts(APCs)for the gutted and whole Pacific saury reached 7.0 log10 CFUg^-1 on days 6 and 10,respectively.Organoleptic,biochemical and microbiological analyses revealed that shelf-lives should be 6–8 days for gutted fish and 10 days for whole fish.Microbiota analysis results showed that gutting partly changed the initial microbiota,but didn't alter the dominant bacteria during storage.When the fish were spoiled,high proportion of Pseudomonadaceae was detected in both groups.     

宽频带静电反馈倾斜仪正交耦合误差分析

分析和计算宽频带静电反馈倾斜仪测量中正交方向上的倾斜对测量结果的影响。结果表明,正交方向的倾斜使测量结果产生误差,需对仪器加以改进,以减小正交耦合误差。     

Influencing factors of seed long-distance dispersal on a fragmented forest landscape on Changbai Mountains,China

Seed long-distance dispersal(LDD) events are typically rare, but are important in the population processes that determine large-scale forest changes and the persistence of species in fragmented landscapes. However, previous studies focused on species dispersed via animal-mediated LDD, and ignored those dispersed by wind. The aim of this study was to assess the effects of canopy openness, edge, seed source, and patch tree density on the LDD of seeds by wind in forest. We collected birch seeds, a typical wind-dispersed species, throughout a larch plantation. We then assessed the relationship between birch LDD and each factor that may influence LDD of seeds by wind including distance to edge, canopy openness size, distance to mature forest, and the tree density of the larch plantation. We used univariate linear regression analysis to assess the influence of those factors on birch LDD, and partial correlations to calculate the contribution of each factor to LDD. The results showed that both canopy openness and edge had significant influences on birch LDD. Specifically, a negative relationship was observed between distance to edge and birch LDD, whereas there was a positive correlation between canopy openness size and LDD. In contrast, the distance to the mature forest was not correlated with birch LDD. Our results suggest that patch tree density could potently affect the probability of LDD by wind vectors, which provides novel and revealing insights regarding the effect of fragmentation on wind dynamics. The data also provide compelling evidence for the previously undocumented effect of habitat fragmentation on wind-dispersed organisms. As such, these observations will facilitate reasonable conservation planning, which requires a detailed understanding of the mechanisms by which patch properties hamper the delivery of seeds of wind-dispersed plants to fragmented areas.     

Development of three multiplex PCR primer sets for ark shell (<Emphasis Type="Italic">Scapharca broughtonii</Emphasis>) and their validation in parentage assignment

Scapharca broughtonii is a commercially important and over-exploited species. In order to investigate its genetic diversity and population structure, 43 novel polymorphic microsatellites were isolated and characterized. The number of alleles per locus ranged from 3 to 22 with an average of 6.93, and the observed and expected heterozygosities varied between 0.233 and 1.000, and 0.250 and 0.953, with an average of 0.614 and 0.707, respectively. Three highly informative multiplex PCRs were developed from nine of those microsatellites for S. broughtonii. We evaluated and validated these multiplex PCRs in 8 full-sib families. The average polymorphism information content (PIC) was 0.539. The frequency of null alleles was estimated as 3.13% of all the alleles segregation based on a within-family analysis of Mendelian segregation patterns. Parentage analysis of real offspring demonstrated that 100% of all offspring were unambiguously allocated to a pair of parents based on 3 multiplex sets. Those 43 microsatellite loci with high variability will be helpful for the analysis of population genetics and conservation of wild stock of S. broughtonii. The 3 sets of multiplex PCRs could be an important tool of pedigree reconstruction, population genetic analysis and brood stock management.     

An AFLP Genetic Linkage Map of Pacific Abalone （Haliotis discus hannai）

A genetic linkage map of Pacific abalone （Haliotis discus hannai） was constructed using AFLP markers based on a two-way pseudo-testcross strategy in a full-sib family. With 33 primer combinations, a total of 455 markers （225 from the female parent and 230 from the male parent） segregated in a 1 ： 1 ratio, corresponding to DNA polymorphism： heterozygous in one parent and null in the other. The female framework map consisted of 174 markers distributed in 18 linkage groups, equivalent to the H. discus hannai haploid chromosome number, and spanning a total length of 2031.4 cM, with an average interval of 13.0 cM between adjacent markers. The male framework map consisted of 195 markers mapped on 19 linkage groups, spanning a total length of 2273.4cM, with an average spacing of 12.9cM between adjacent markers. The estimated coverage for the framework linkage maps was 81.2% for the female and 82.1% for the male, on the basis of two estimates of genome length. Fifty-two markers （11.4%） remained unlinked. The level of segregation distortion observed in this cross was 20.4%. These linkage maps will serve as a starting point for linkage studies in the Pacific abalone with potential application for marker-assisted selection in breeding programs.     

Variation of Floods Characteristics and Their Responses to Climate and Human Activities in Poyang Lake,China

The Poyang Lake is one of the most frequently flooded areas in China. Understanding the changing characteristics of floods as well as the affecting factors is an important prerequisite of flood disaster prevention and mitigation. The present study identified the characteristics variations of historical floods in the Poyang Lake and their tendencies based on the Mann-Kendall(M-K) test, and also investigated the related affecting factors, both from climate and human activities. The results revealed that the highest flood stages, duration as well as hazard coefficient of floods showed a long-term increasing linear trend during the last 60 years with the M-K statistic of 1.49, 1.60 and 1.50, respectively. And, a slightly increasing linear trend in the timing of the highest stages indicated the floods occurred later and later during the last six decades. The rainfall during the flood season and subsequent discharges of the Changjiang(Yangtze) River and runoff from the Poyang Lake Basin were mainly responsible for the severe flood situation in the Poyang Lake in the 1990 s. In addition, the intensive human activities, including land reclamation and levee construction, also played a supplementary role in increasing severity of major floods. While, the fewer floods in the Poyang Lake after 2000 can be attributed to not only the less rainfall over the Poyang Lake Basin and low discharges of the Changjiang River during flood periods, but also the stronger influences of human activity which increased the floodwater storage of the Poyang Lake than before.     

Inheritance Pattern of Microsatellite Loci and Their Use for Kinship Analysis in the Japanese Scallop Patinopecten yessoensis

The inheritance mode of seven microsatellite markers was investigated in Patinopecten yessoensis larvae from four con-trolled crosses,and the feasibility of using these markers for kinship estimation was also examined. All the seven microsatellite loci were compatible with Mendelian inheritance. Neither sex-linked barriers to transmission nor major barriers to fertilization between gametes from the parents were evident. Two of the seven loci showed the presence of null alleles in two families,suggesting the...     

Omni-directional distribution patterns of montane coniferous forest in the Helan Mountains of China

Slope aspect has significant effect on the development and distribution of montane forest,especially in arid and semiarid regions.This paper,using SPOT5 images and 1:50,000 DEM,digitally extracts and analyzes the spatial information of montane coniferous forest(mainly Qinghai spruce),and thereby explores how the upper and lower limits and the altitudinal range of coniferous forest vary and how the area of coniferous forest is related with annual insolation with all aspects in the Helan Mountains.The results show that: 1) In the eastern flank,the lower limit of coniferous forest is between 1,600 m and 2,000 m a.s.l.,and the upper limit between 2,800 m and 3,000 m a.s.l.;in the western flank,the lower limit of coniferous forest is between 2,000 m and 2,300 m a.s.l.,and the upper limit between 2,800 m and 3,100 m a.s.l.2) The altitudinal ranges of coniferous forest are 806~1,435 m,438~1,140 m for eastern flank and western flank,respectively.3) The area of coniferous forest takes on a normal distribution with aspect,and it has a close relationship with annual insolation.This distribution model developed in this paper quantitatively reveals the significant effect of slope aspect on the distribution of coniferous forest in arid and semi-arid land.     

天山地区GPS连续站高程时间序列分析

利用UNAVCO提供的天山地区境内外GPS连续站的观测数据,处理得到各站高程时间序列。进行最小二乘线性拟合,提取速度场,并消除原始序列的线性趋势。然后对时间序列进行谱分析以及小波多分辨率分析。可以看出,天山地区在过去10余年间呈现整体隆升趋势,各个观测站之间抬升的一致性较好,并且各站都表现出明显的季节性变化。