Development of 57 novel polymorphic microsatellite markers in half-smooth tongue sole (Cynoglossus semilaevis)

Half-smooth tongue sole (Cynoglossus semilaevis) is a promising species for aquaculture in China.The wild population of C. semilaevis is under threat from environmental factors. Microsatellite markers are very suitable for assessing genetic diversity. Four microsatellite-enriched libraries of half smooth tongue sole (Cynoglossus semilaevis) were constructed,from which 57 polymorphic microsatellites were isolated and characterized.The polymorphism of these microsatellites was assessed by genotyping in 30 ind...     

城市空间智能计算平台研究

该文提出了空间智能平台的概念,阐述了其研究意义和内容,明确了空间智能计算平台核心计算模型的建模对象和目标。在此基础上,分析了宏观数学模型、群体行为模型两种用来构建空间活动模型的建模方法以及适用于空间活动分析的物理环境建模技术。从融合多尺度行为模型、构建城市日常生活模型、验证和校验核心模型以及平衡效率和效果4方面探讨了构建空间智能计算平台的关键技术。从实际功能看,城市空间智能平台是联系宏观复杂问题和微观机制的桥梁,是分析城市问题的工程化实验平台,具有广阔的应用前景。     

Impact of the variation of westerly jets over East Asia on precipitation of eastern China in July

The impact of the northward jump and westward movement of the East Asian westerly jet core from the western Pacific Ocean to the Qinghai-Tibet Plateau on precipitation distribution of eastern China is studied. It is concluded that on the one hand, the northward jump of the jet causes the precipitation belt to move northward from the middle and lower reaches of the Yangtze River valley and withdraw during the Mei-yu season; on the other hand, the westward movement of the jet core has no correspondence with withdrawal of the Mei-yu season. However, the earlier or later occurrence of the westward movement of the jet has an influence on the process of the rain belt moving northward than the northward jump of the jet: the rain belt moves northward from the middle-lower Yangtze River valley to the Huaihe River and then to an area between the Yellow River and Huaihe River during years when the time of the westward movement of the jet core is later than that of the northward jump of the jet and from the middle-lower Yangtze River valley to an area between the Yellow River and Huaihe River in other years. Further analysis shows that: (1) The northward jump of the jet and the westward movement of the East Asian westerly jet core causes significant variation of the general atmospheric circulation in middle latitudes and water vapor transport from the western Pacific, but not from the Bay of Bengal. (2) Impact of the northward jump and the westward movement of the East Asian westerly jet core on circulation are different, therefore, water vapor transport from the western Pacific and its impact on the rain belt are different. The earlier or later occurrence of the westward movement of the jet core than the northward jump of the jet causes the process of circulation and water vapor transport to be different which produces a different process of the rain belt moving northward.     

真鲷（Pagrus major）vasa基因5′端启动子克隆及表达载体构建

采用染色体步移（Genome walking）的方法克隆了真鲷vasa基因5′侧翼序列,采用生物信息学方法分析潜在的顺式作用元件,并与斑马鱼核心启动子进行比对,在此基础上构建了绿色荧光蛋白表达载体。序列分析结果显示：克隆得到的真鲷vasa基因5′侧翼序列序列长度为2762bp,其中包括TATA-box、CAAT-box...     

Accumulation of heavy metals in wild commercial fish from the Baotou Urban Section of the Yellow River,China

In this work we studied the accumulation of heavy metals in nine species of fish with different life and feeding habitats which are native and major commercial fish in the Baotou Urban Section of the Yellow River. The results showed that the concentration of heavy metals was significantly dependent on fish species; the pollution index of heavy metals in different species were ranked as Hemiculter leucisclus > Carassius auratus auratus > Hemibarbus maculatus > Megalobrama amblycephala > Abbottina rivularis > Cyprinus carpio > Squaliobarbus curriculus > Perccottus glehni > Saurogobio dabryi. Product–moment correlation coefficients among the metal pairs Pb–Zn, Cu–Cd, Cu–Zn, Cu–Pb, Pb–Cd, and Zn–Cd revealed there was no competitions between metals in each tissue. Correlations between heavy metal concentrations and fish length or weight indicated that accumulation of the heavy metals by the different fish species was related to their surrounding environments and their life and feeding habitats. According to the mean bioconcentration factors (BCFs), the heavy metal concentrations in these nine species were ranked Zn ≫ Cu > Cd ≈ Pb. In this work, the bioaccumulation factors (BAFs) were developed by using the sum of exchangeable and bound-to-carbonate heavy metals as Cs values. It was found that BAFs better reveal the accumulation characteristics of the heavy metals in the fish, which might provide an effective method for assessing bioaccumulation of heavy metals.     

Optical characterization of CDOM in a marsh-influenced environment in the Changjiang (Yangtze River) Estuary

To elucidate the influence of tidal marshes on the amount and chemical structure of chromophoric dissolved organic matter (CDOM) in adjacent coastal waters, surface water samples were collected along three cross-marsh transects in tidal marshes within the Changjiang Estuary. In addition, three sediment cores were collected from the marshes and incubated for 10 h in the laboratory after adding overlying tidal waters. Nutrients, DOC, absorption, fluorescence excitation–emission matrix (EEM) spectroscopy, and other parameters were measured in these samples, which clearly illustrated releases by the tidal marshes of DOC, and CDOM absorbent and fluorescent molecules. To characterize the chemical nature of the marsh-derived CDOM, variations in a suite of optical indices (including molar absorptivity, absorption spectral slope, fluorescence quantum yield, and fluorescence ratios between certain excitation/emission locations) in these samples were plotted against the offshore distance of the stations as well as the incubation time. From the literature, it has been suggested that these variations are closely associated with molecular weight, aromaticity, and humic/fulvic or allochthonous/autochthonous ratios in the CDOM mixtures. This study showed that the CDOM fluorophores changed to more humic-like and were less protein-like from sea to land across the marsh gradients. Furthermore, subtractions between EEM spectra before and after laboratory incubations were applied to illustrate in greater detail the properties of the pure fluorescent compounds exchanged at the sediment–overlying water interface.     

Shallow water depth retrieval from space-borne SAR imagery

Based on shallow water bathymetry synthetic aperture radar (SAR) imaging mechanism and the microwave scattering imaging model for oceanic surface features, we developed a new method for shallow water depth retrieval from space-borne SAR images. The first guess of surface currents and winds are estimated from the normalized radar crossing section (NRCS) profile of shallow water bathymetry SAR imagery, according to the linear theory and geophysical model function. The NRCS profile is then simulated by the microwave scattering imaging model. Both the surface currents and winds are adjusted by using the dichotomy method step by step to make the M4S-simulated NRCS profiles approach those observed by SAR. Then, the surface currents and the wind speeds are retrieved when a best fit between simulated signals and the SAR image appears. Finally, water depths are derived using the Navier–Stokes equation and finite difference method with the best estimated currents and the surface winds. The method is tested on two SAR images of the Taiwan Shoal. Results show that the simulated shallow water NRCS profile is in good agreement with those measured by SAR with the correlation coefficient as high as 85%. In addition, when water depths retrieved from the SAR image are compared with in situ measurements, both the root mean square and relative error are less than 3.0 m and 6.5%, respectively, indicating that SAR images are useful for shallow water depth retrieval and suggesting that the proposed method in this paper is convergent and applicable.     

A new ketosteroid from red alga Acanthophora spicifera

A new ketosteroid, along with six known steroids, was isolated from the ethanolic extracts of red alga Acanthophora spicifera (Vahl.) Boergesen. The structures, identified using chemical and spectroscopic methods including 2D NMR, were: (1) 22-hydroxy-5α-cholest-3,6-dione, (2) 6-hydroxycholest-4-ene-3-one, (3) cholest-4-ene-3,6-dione, (4) cholest-5-ene-3β-ol, (5) 5α-cholestane-3,6-dione, (6) β-Sitosterol and (7) Saringosterol. The MTT method was used to test the cytotoxicity of the compounds against the human cancer cell lines, HCT-8, Bel-7402, BGC-823, A549 and HELA. Compounds 1, 2, 3 and 5 showed moderate cytotoxic activity against human cancer cell lines.     

Evaporative enrichment of oxygen-18 and deuterium in lake waters on the Tibetan Plateau

Stable isotopes (δ¹⁸O and δD) are useful tracers for investigating hydrologic and climatic variability on a variety of temporal and spatial scales. Since the early isotopic studies on mountainous glaciers in the late 1960s, a great deal of information has been generated on the isotopic composition of rainfall, snow, ice, surface waters, and lake carbonate sediments across the Tibetan Plateau. However, measurements of δ¹⁸O and δD values of lake water are scarce. Here we present a new dataset of δ¹⁸O and δD values of lake waters collected from 27 lakes across the plateau during a reconnaissance survey in summer 2009. δ¹⁸O and δD values of lake water range from −19.9 to 6.6‰ and from −153 to −16‰, respectively. The average values of δ¹⁸O and δD are −6.4 and −72‰, considerably greater than those of precipitation observed in this region. The derived Tibetan lake water line, δD = 5.2δ¹⁸O − 38.9, is significantly different from the global meteoric water line. Most of the lakes, including some freshwater lakes, contain water with negative values of d-excess (d). There is a negative correlation between d and total dissolved solids (TDS). Each of these findings indicates that evaporation-induced isotopic enrichment prevails in Tibetan lakes. Moreover, we develop an isotope modeling scheme to calculate E/P ratios for Tibetan lakes, using a combination of existing isotopic fractionation equations and the Rayleigh distillation model. We use the intersection of the local evaporation line and GMWL as a first approximation of δ¹⁸O and δD values of lake water inputs to infer an E/P ratio for each lake. Our modeling calculations reveal that although variable from lake to lake, the water budget across the plateau is positive, with an average E/P of 0.52. This is in good agreement with other observational and model data that show varying degrees of increases in lake size from satellite imagery and significant decreases in lake salinity in many lakes on the plateau over the last several decades. Together with the new isotopic dataset, the proposed modeling framework can be used to examine and quantify past changes in a lake’s hydrologic balance from the isotopic record of downcore carbonate sediments in the region.     

湖北冷季高架雷暴天气分类及环境参数特征分析

利用常规观测资料、加密地面自动站、闪电定位仪和ERA5再分析资料,将2011—2020年湖北冷季91次高架雷暴事件,按照形成机制分为雷雨型、强对流型和雷打雪型3类,并细致归纳了3类事件的时空分布特征、大气环流背景及关键环境参数等特征。(1) 湖北冷季高架雷暴雷雨型最多,强对流型次之,雷打雪型最少。3类型存在明显的时空分布差异,雷雨型主要发生在秋末冬初(11月)和冬末初春(1—2月),强对流型及雷打雪型常出现在早春2月,3月基本以强对流型为主。(2) 低槽冷锋、850 hPa切变线及低空西南急流是冷季高架雷暴发生的有利环流背景,近地面为稳定的冷气团控制,逆温明显,西南低空急流沿着锋面逆温层以上的850 hPa附近触发抬升,水汽、上升运动及不稳定层结均出现在850 hPa以上。雷雨型和雷打雪型距离冷锋超过100 km以上,强对流型不足100 km。(3) 850 hPa是风场转换的重要层次。强对流型850 hPa露点温度(Td850)、K指数、850 hPa与 500 hPa温差(ΔT85)、850 hPa假相当位温(θse850)、西南急流厚度和强度(I700)、切变线强度(S850)最大,中低层(850~700 hPa)垂直风切变(SL78)最小;雷打雪型对水汽和不稳定能量的要求最低,SL78最大。