南极适冷菌Psychrobacter sp.G 普遍胁迫蛋白(USP)基因的克隆及其胁迫条件下的应答特征分析

根据南极适冷菌Psychrobacter sp.G基因组测序草图发现并克隆到了1个普遍胁迫蛋白(Universal StressProtein,USP)基因Usp1141。该基因ORF长462bp,编码153个氨基酸,蛋白理论分子量为17kDa,pI为5.23。通过多序列比对发现该USP含有交替排列的4个α螺旋和5个β折叠,以及与ATP结合相关的氨基酸残基,表明该蛋白可能通过与ATP结合而发挥作用。利用实时定量PCR技术对该蛋白在不同胁迫条件下的表达情况进行分析发现,温度变化对Usp1141基因的表达没有显著影响;低盐度(0,15)胁迫会显著抑制其表达,而高盐度(90,120)胁迫则会显著促进其表达;在温度与盐度协同胁迫条件下,当培养基盐度低于菌株生长的最适盐度时,无论温度高低,Usp1141基因的表达均受到抑制,而当培养基盐度高于最适盐度时,无论温度高低,Usp1141基因的表达均会提高。实时定量PCR研究表明,Usp1141基因可能在南极适冷菌Psychrobactersp.G对环境渗透压变化的适应性中发挥作用。     

渤、黄、东海潮汐、潮流的数值模拟与研究

基于FVCOM海洋数值模式,采用高分辨率的三角形网格,对渤、黄、东海的潮汐、潮流进行数值模拟,并通过比较120个沿岸验潮站和14个潮流观测站的实测与模拟结果进行模型验证,两者符合较好。根据模拟结果,给出了四个主要分潮的潮汐同潮图和5m层潮流最大流速及最大潮流同潮时分布。渤、黄、东海共有5个半日分潮和3个全日分潮的独立旋转潮波系统,且都呈逆时针方向旋转;半日潮流和全日潮流各有12个圆流点;在冲绳岛和奄美岛两侧的4个半日潮流圆流点分别呈对称分布,其中有3个为本文首次给出;在日本九州岛西侧还新给出2个全日潮流圆流点。有关它们的存在性需要实测资料的进一步检验。     

渤、黄、东海三维潮波运动数值模拟

采用POM三维海流数值模式模拟渤、黄、东海潮波运动。所得潮汐调和常数与77个测站的观测值的平均绝对误差：m1振幅1．92cm,迟角4．39°;M2振幅4．70cm,迟角5．08°。模拟结果显示M2分潮流共存在8个圆流点,m1岁潮流共存在9个圆流点。其中,M2分潮流在舟山群岛附近与m1分潮流在黄海北部和台湾海峡谷存在一个圆流点,这三个圆流点是本文数值模拟所首次显示的,需进一步的观测验证它们实际存在与否。模式还得出湍流涡动系数分布。     

基于多波束前视声呐的水下静态目标的探测识别技术

基于声呐的水下目标检测是具有重要意义的研究课题.由于声呐图像质量差、对比度低、边缘模糊等,基于特征提取的识别方法在精度和速度上无法满足现有要求.鉴于此,将基于卷积神经网络的YOLOv3引入声呐图像的目标识别任务中,首先通过动态亮度分配和中值滤波对图像进行预处理建立数据集,然后对YOLOv3模型进行训练和测试,最后根据静...     

柘林湾表层沉积物重金属分布及污染初步评价

研究了柘林湾表层沉积物样品中7种重金属元素的质量分数及分布特征,并对其来源进行了分析。结果显示,调查海区Zn的平均质量分数为113.0μg/g±6.5μg/g、Cr为40.1μg/g±8.5μg/g、Mn为837.9μg/g±168.6μg/g、Cu为24.0μg/g±4.3μg/g、Pb为57.7μg/g±11.3μg/g、Ni为24.4μg/g±5.7μg/g、Fe为3.7%±0.4%。重金属主要来源于陆源风化产物,其质量分数反映了粤东地区的地质特点。分别以沉积物质量和香港环保署公布的标准,对柘林湾表层沉积物重金属污染进行了评估,结果表明:只有Pb在部分站位达到中度污染水平,其它重金属元素则只为轻度污染。     

基于生态化人工海岸建设的理论研究与应用 ——以天津永定新河口综合整治修复工程为例

分析生态文明背景下围填海区域人工海岸建设存在的问题,结合淤泥质人工海岸空间特点,总结提出了生态岸线整治修复技术、仿自然化处理设计、景观构建适宜性、人工海岸生态系统等生态化建设基础理论,并以天津永定新河口综合整治修复工程为例,重点阐述了人工海岸生态化建设过程中生态系统和景观构建的方法要点和具体思路.结果表明:在人工海岸生...     

顾及水深的单波束测深系统误差改正方法

为解决单波束测深系统利用单一延迟值改正往返断面位移精度低的问题,在分析单波束测深系统误差产生原理基础上,得出往返断面位移是由系统延迟引起的固定差和换能器偏角引起的与深度相关比例误差共同作用的结果,通过建立水深与往返断面位移之间的数学模型,证实水深与断面位移具有极高相关性.利用求取单波束系统延迟及换能器偏角参数,对同一测...     

海洋表层温度对台风"蔷薇"路径和强度预测精度的影响

基于中尺度大气模式WRF(Weather Research and Forecasting Model),首先对2007年3次船舶辐射通量观测进行模拟,以检验WRF对长波和短波辐射通量的模拟能力,结果表明使用中国近海海洋环境数值预报系统环流模式POM(Princeton Ocean Model)模拟的高时空分辨率的海洋表层温度能够显著改进短波辐射通量的模拟,而对长波辐射通量模拟的改进不明显。然后,将业务化运行的中国近海海洋环境数值预报系统后报的逐时海洋表面温度(SST)作为WRF底边界条件,对2008年15号强台风"蔷薇"(Jangmi)过程进行了数值后报试验。结果表明,与使用NCEP/NCAR的SST试验后报的台风中心位置偏差相比,使用高时空分辨率的SST能够较为显著地改善"蔷薇"的路径模拟,台风中心位置模拟偏差减少11%,尤其在台风减弱阶段,台风中心位置模拟偏差减少37%。台风强度在台风发展的不同阶段对下垫面SST的变化敏感性不同。台风路径附近的海表面温度下降会导致海洋向大气输送的热量减少从而减弱台风强度。     

Distribution of major and trace elements in surface sediments of Hangzhou Bay in China

The Inductively Coupled Plasma Optical Emission Spectrometer was used to analyze sediment samples collected from the Hangzhou Bay to determine major and trace elemental concentrations. Based on these concentrations, the study area can be classified into three geochemical provinces. Province I covers the northern Hangzhou Bay area and contains high concentrations of Al₂ O₃ , Fe₂ O₃ , MgO, Na₂ O, K₂ O, MnO, Cr, Cu, Ni, Pb, V, Co and Zn. Province III is located in the western Hangzhou Bay, near the Qiantang River mouth, and contains high concentrations of SiO₂ , Na₂ O, P₂ O₅ , TiO₂ , Cr, Sr, Zr. Province II is located in the middle and eastern Hangzhou Bay, with the medium concentrations of major and trace elements. The results also demonstrate that the grain size is the dominating factor controlling the spatial variations of elemental concentrations, and the Changjiang River （Yangtze River） and Qiantang River sediments play an important role in the distribution of these elements. Anthropogenic impact on heavy metal concentrations （especially Cr, Sr and Zr） can be detected in the surface sediments near the Qiantang River mouth.     

Smectite formation in metalliferous sediments near the East Pacific Rise at 13°N

A 43 cm long E271 sediment core collected near the East Pacific Rise(EPR) at 13°N were studied to investigate the origin of smectite for understanding better the geochemical behavior of hydrothermal material after deposition.E271 sediments are typical metalliferous sediments. After removal of organic matter, carbonate, biogenic opal,and Fe-Mn oxide by a series of chemical procedures, clay minerals(2 μm) were investigated by X-ray diffraction,chemical analysis and Si isotope analysis. Due to the influence of seafloor hydrothermal activity and close to continent, the sources of clay minerals are complex. Illite, chlorite and kaolinite are suggested to be transported from either North or Central America by rivers or winds, but smectite is authigenic. It is enriched in iron, and its contents are highest in clay minerals. Data show that smectite is most likely formed by the reaction of hydrothermal Fe-oxyhydroxide with silica and seawater in metalliferous sediments. The Si that participates in this reaction may be derived from siliceous microfossils(diatoms or radiolarians), hydrothermal fluids, or detrital mineral phases. And their δ30 Si values are higher than those of authigenic smectites, which implies that a Si isotope fractionation occurs during the formation because of the selective absorption of light Si isotopes onto Feoxyhydroxides. Sm/Fe mass ratios(a proxy for overall REE/Fe ratio) in E271 clay minerals are lower than those in metalliferous sediments, as well as distal hydrothermal plume particles and terrigenous clay minerals. This result suggests that some REE are lost during the smectite formation, perhaps because their large ionic radii of REE scavenged by Fe-oxyhydroxides preclude substitution in either tetrahedral or octahedral lattice sites of this mineral structure, which decreases the value of metalliferous sediments as a potential resource for REE.