海州湾海域赤潮灾害与成因探讨

2004～2006年海州湾海域共发生有记录的赤潮7起,涉及海域面积近2000 km2,海洋生态环境受到严重威胁.本文根据近年来海州湾赤潮发生的情况和相关资料,阐述了海州湾赤潮的发生及其影响、赤潮主要藻种,从物理、化学、气候、生物等因素上分析和讨论了海州湾赤潮发生的原因,并在此基础上提出了相应的防治对策:(1)加强赤潮的研究工作;(2)控制陆源污染物的入海,防止海水富营养化;(3)加强赤潮监测.     

Primary molecular basis of androgenic gland endocrine sex regulation revealed by transcriptome analysis in Eriocheir sinensis

In crustaceans, the male sexual dif ferentiation and maintenance are specially regulated by androgenic gland(AG). However, little is known about the genes involved in the regulation process.RNA-Seq was performed on AG with ejaculatory duct(AG_ED) and ejaculatory duct(ED) as control in Eriocheir sinensis, one of the most important economic and ?shery crabs with typically sex dimorphism. A total of 925 unigenes were identi?ed as dif ferentially expressed genes(DEGs) and the expression of nine genes randomly selected was con?rmed by qRT-PCR. 667 unigenes were up-regulated in AG_ED, being supposed to be AG preferential genes. Among them, the full length of i nsulin-like androgenic gland factor( IAG) cDNA named as Es-IAG was obtained as a logo gene of AG, which together with the genes i nsulin-like receptor( INR), and s ingle insulin binding domain protein( SIBD), might constitute the sex regulation pathway. Several sex related genes were identi?ed, and their function will have to be investigated. Also,the identi?cation of j uvenile hormone epoxide hydrolase 1( JHEH1), ecdysteroid 22-hydroxylase( DIB) and e cdysone receptor( ECR) preliminarily clari?ed the molecular regulation mechanism of eyestalk-AG-testis axis, which plays important roles in molting and reproduction. The results will enhance our understanding for the molecular basis of the AG involved in male sex regulation in crabs.     

MALBAC技术扩增微生物群落基因组的效率评估

环境样品的低生物量是微生物宏基因组学研究面临的首要挑战,通过基因组扩增技术来满足高通量测序对DNA样品量的需求是最常用的解决策略。MALBAC(Multiple Annealing and Looping Based Amplification Cycles)基因组扩增试剂盒最初为扩增和研究哺乳动物的单细胞基因组而研发。本文中,我们通过人工构建的微生物群落来检测该试剂盒在微生物宏基因组扩增方面的效率和应用可行性。结果表明,每个标准反应中,10 pg的DNA模板量足以满足MALBAC试剂盒对样品扩增的需要。每个标准反应DNA模板用量为10和100 pg时,所扩增DNA样品的基因组覆盖度与原始未扩增样品表现出高度的一致性,证明MALBAC试剂盒扩增效果的高度稳定性和一致性。常用的GenomePlex全基因组扩增试剂盒使我们可以在每个标准反应DNA模板量为100 pg的条件下扩增获得足够的DNA样品,但是结果表明该参照试剂盒无法有效的实现对群落中低丰度细菌菌株基因组的线性扩增。对于MALBAC试剂盒和参照试剂盒而言,在扩增高GC含量的微生物物种基因组DNA方面效率低下。我们的实验结果表明MALBAC试剂盒在高效扩增环境样品宏基因组DNA方面的可行性,但对该试剂盒在扩增环境样品中高GC含量微生物物种方面的适用性存在疑虑。     

潮流界以下河段航道整治水位确定方法研究——以长江江阴以下河段为例

在总结分析现有整治水位确定方法的基础上,根据潮流界以下河段的水沙运动特性,本文提出一种基于输沙能力的航道整治水位确定方法。考虑上游来水、下游潮汐为独立事件,统计潮流界以下河段上游来水、下游潮汐不同等级组合出现的频率,采用数学模型计算相应组合下河段沿程的潮位、流速过程,以流速四次方代表水流的输沙能力,统计不同潮位等级对应的综合净输沙能力,确定最大综合净输沙能力对应的水位为(最优)航道整治水位。以长江下游白茆沙水道和福姜沙水道为例,计算了所在河段的航道整治水位,并探讨了起动流速对整治水位计算的影响和最高整治水位概念对工程的意义。     

Numerical study of sand scour with a modified Eulerian model based on incipient motion theory

Application of the standard Eulerian model to simulations of sand scour results in unrealistic phenomena. Therefore, the present work develops a modified Eulerian model based on sand incipient motion theory. The modified model is applied for simulating a two-dimensional single vertical jet and a moving planar jet. The simulation results generally demonstrate fairly good agreement with published results of scour profiles and the velocity contours of the water and sand phases. In addition, equations to describe self-similar scour profiles for the moving planar jet cases are given. The results demonstrate that the modified model efficiently and accurately simulates the two-dimensional sand scour produced by jets, particularly for the moving jet cases.     

大连市滨海湿地现状及加强管护工作对策研究

文章利用遥感技术获取了大连市2016年滨海湿地基本状况信息,通过实地调查和查阅资料基本摸清了研究区水鸟及栖息地概况,收集整理了研究区开展滨海湿地管理与保护工作情况,简要阐释了当前滨海湿地管护工作中存在的功能退化等问题,辨识性分析了引发问题的填海造地、建设力度与保护经费不足等驱动力因素,提出了应从健全滨海湿地管理与保护制度机制、加大新建保护区与已建保护区管护工作力度、开展生态修复工作、开展滨海湿地生态补偿工作、严格自然滨海湿地开发利用管理和加强滨海湿地调查监测6个方面加强滨海湿地管护工作的对策及建设性意见。     

pH值对单环刺螠呼吸排泄的影响

为研究pH值对单环刺螠(Urechis unicinctus)呼吸排泄的影响,采用静水密封法,研究了不同pH值(5、6、7、8、9)对体质量5.65±1.06g单环刺螠耗氧率、排氨率、能量代谢率、O/N值的影响.结果表明,pH值对单环刺螠耗氧率、排氨率、能量代谢率、O/N值均有显著影响(p0.05).单环刺螠耗氧率随pH值的升高而增大,pH值为8时达到最大值,之后随pH值的升高而减小.单环刺螠耗氧率与pH值之间的拟合方程为:OR=-2.977X2+55.188X-176.380,R2=0.937.单环刺螠排氨率的变化与耗氧率相似,在pH值为7时排氨率达到最大值;pH值为9时排氨率最小,与pH值为8的组差异不显著(p0.05).排氨率与pH值之间的拟合方程为:NR=-0.350X2+4.442X-6.822,R2=0.724.单环刺螠能量代谢率、O/N值均随pH值的升高呈先增大后减小的趋势,拟合方程分别为:M=-0.040X2+0.424X-0.044,R2=0.937;A=0.053X2+1.879X-7.470,R2=0.901.pH值为8的环境中,单环刺螠体内蛋白质代谢水平最低,有利于蛋白质的积累.研究表明,单环刺螠在一定pH值范围内可以通过调整生理代谢水平适应水体pH值,其适宜生活在弱碱性水中,生存最适pH值为8.     

热带西太平洋Y3和M2海山微食物网主要类群生态分布与比较

2014年12月和2016年3月分别对热带西太平洋Y3海山(中层海山)和M2海山(浅海山)微食物网主要类群(包括聚球藻、原绿球藻、微微型真核浮游生物、异养细菌和浮游纤毛虫)丰度和生物量垂直分布进行了研究。结果表明,Y3和M2海山水文环境比较相似但略有区别,叶绿素最大值层(DCM)分别在75—100m和110m水层,微食物网各主要类群在垂直尺度上的分布与叶绿素a浓度紧密相关。其中浮游纤毛虫呈现"双峰型"模式,即丰度高值出现在表层和DCM层;原绿球藻和微微型真核浮游生物呈现"单峰型"模式,丰度高值出现在DCM层;聚球藻和异养细菌峰型相对不显著,DCM层以浅丰度较高,DCM层以深丰度明显降低。分析其原因,可能是受到温度、光照和营养盐的共同影响。Y3和M2海山微食物网结构的垂直变化不完全一致。其中,Y3海山30m以浅和150m以深异养细菌生物量占绝对优势,75—100m水层自养型生物(原绿球藻和微微型真核浮游生物)占绝对优势;M2海山75m以浅和200m以深异养细菌占绝对优势,110—150m自养型生物占绝对优势。M2海山自养型生物占优势的水层要明显深于Y3海山,可能与它们的海山类型和采样季节不同有关。     

GC-MS联合二维核磁对文蛤多糖Fr.2A组分结构的解析

采用GC-MS联合二维核磁的方法,对文蛤均多糖结构进行解析。运用双酶辅助水提的方法,提取文蛤粗多糖(MMPX),分离纯化得到文蛤均多糖,并通过气质联用技术(GC-MS)和核磁共振波谱(1D,2D NMR),对文蛤均多糖的结构进行了分析。结果表明,从文蛤中提取得到文蛤粗多糖(MMPX),经分离纯化得到高纯度文蛤均多糖Fr.2A,其单糖组成为葡萄糖(Glc)。GC-MS分析发现,Fr.2A存在三种残基单元1,4-Glcp、T-Glcp和1,4,6-Glcp,摩尔比为5.58︰1.26︰1.0;核磁共振波谱分析表明,Fr.2A是以α-(1→4)与少量α-(1→6)连接为主链,并存在少量的α-(1→6)和β-(1→4)支链的新型水溶性D型吡喃葡聚糖。     

Numerical simulation of longitudinal settlement of shield tunnel in the coastal city Shanghai

Until now more than 14 subway lines are in operation and some new lines are being built in the coastal city Shanghai. The longitudinal settlement of shield tunnel has significant effect on the safety of the subway operation. In this paper, the deformation of the shield tunnel and the surrounding soil were analyzed by the establishment of a three-dimensional model. The vertical displacements of four paths (Path 1 is on the ground; Path 2 is at the top of the tunnel; Path 3 is in the middle of the tunnel; Path 4 is at the bottom of the tunnel) are affected by the nature of the soil. The horizontal displacement is smaller than the vertical displacement and horizontal displacement of the clay is larger than that of the sand. The distribution of the pore pressure changes with soil properties around the tunnel. The pore pressure of the sand layer is larger than that of the clay layer at the same depth of underlying soil.