为从一种组成未知的商用复合菌中分离并筛选出优势发酵菌株,本实验采用稀释平板培养法,对菌种进行分离纯化,同时参照《真菌鉴定手册》和《常见细菌鉴定手册》,根据其形态学特征、生理生化特性,并分别结合16S r DNA和18S r DNA序列的比对分析对菌种进行鉴定。另外,本研究通过生长曲线测定,单菌种发酵试验以及多菌种混合发酵发酵试验,以游离氨基酸态氮为指标,以期筛选出发酵低值水产品制备菌肥的最佳菌株组合。结果显示,共分离得到4株菌,分别为a热带假丝酵母、b罗伦隐球菌、c枯草芽孢杆菌、d蜡样芽胞杆菌。且菌种组合acd发酵后游离氨基酸态氮的含量较对照组上升最显著,为8.276g/L。故可确定最佳发酵菌株配方为acd(热带假丝酵母+枯草芽孢杆菌+蜡样芽胞杆菌)。 相似文献
During the self-weight penetration process of the suction foundation on the dense sand seabed, due to the shallow penetration depth, the excess seepage seawater from the outside to the inside of the foundation may cause the negative pressure penetration process failure. Increasing the self-weight penetration depth has become an important problem for the safe construction of the suction foundation. The new suction anchor foundation has been proposed, and the self-weight penetration characteristics of the traditional suction foundation and the new suction anchor foundation are studied and compared through laboratory experiments and analysis. For the above two foundation types, by considering five foundation diameters and two bottom shapes, 20 models are tested with the same penetration energy. The effects of different foundation diameters on the penetration depth, the soil plug characteristics, and the surrounding sand layer are studied. The results show that the penetration depth of the new suction foundation is smaller than that of the traditional suction foundation. With the same penetration energy, the penetration depth of the suction foundation becomes shallower as the diameter increases. The smaller the diameter of the suction foundation, the more likely it is to be fully plugged, and the smaller the height of the soil plug will be. In the stage of self-weight penetration, the impact cavity appears around the foundation, which may affect the stability of the suction foundation.
Sparsity constrained deconvolution can improve the resolution of band-limited seismic data compared to conventional deconvolution. However, such deconvolution methods result in nonunique solutions and suppress weak reflections. The Cauchy function, modified Cauchy function, and Huber function are commonly used constraint criteria in sparse deconvolution. We used numerical experiments to analyze the ability of sparsity constrained deconvolution to restore reflectivity sequences and protect weak reflections under different constraint criteria. The experimental results demonstrate that the performance of sparsity constrained deconvolution depends on the agreement between the constraint criteria and the probability distribution of the reflectivity sequences; furthermore, the modified Cauchyconstrained criterion protects the weak reflections better than the other criteria. Based on the model experiments, the probability distribution of the reflectivity sequences of carbonate and clastic formations is statistically analyzed by using well-logging data and then the modified Cauchy-constrained deconvolution is applied to real seismic data much improving the resolution. 相似文献
A coupled physical–biological model was developed to simulate the low-silicate, high-nitrate, and low-chlorophyll (LSHNLC) conditions in the equatorial Pacific Ocean and used to compute a detailed budget in the Wyrtki box (5°N–5°S, 180–90°W) for the major sources and cycling of nitrogen and silicon in the equatorial Pacific. With the incorporation of biogenic silicon dissolution, NH4 regeneration from organic nitrogen and nitrification of ammonia in the model, we show that silicon recycling in the upper ocean is less efficient than nitrogen. As the major source of nutrients to the equatorial Pacific, the Equatorial Undercurrent provides slightly less Si(OH)4 than NO3 to the upwelling zone, which is defined as 2.5°N–2.5°S. As a result, the equatorial upwelling supplies less Si(OH)4 than NO3 into the euphotic zone in the Wyrtki box, having a Si/N supply ratio of about 0.85 (2.5 vs. 2.96 mmolm−2 day−1). More Si(OH)4 than NO3 is taken up with a Si/N ratio of 1.17 (2.72 vs. 2.33 mmolm−2 day−1) within the euphotic zone. The difference between upwelling supply and biological uptake is balanced by nutrient regeneration and horizontal advection. Excluding regeneration, the net silicate and nitrate uptakes are nearly equal (1.76 vs. 1.84 mmolm−2 day−1). However, biogenic silica export production is slightly higher than organic nitrogen (1.74 vs. 1.59 mmolm−2 day−1) following a 1.1 Si/N ratio. In the central equatorial Pacific, low silicate concentrations limit diatom growth; therefore non-diatom new production accounts for most of the new production. Higher silicate supply in the east maintains elevated diatom growth rates and new production associated with diatoms dominate upwelling zone. In contrast, the new production associated with small phytoplankton is nearly constant or decreases eastward along the equator. The total new production has a higher rate in the east than in the west, following the pattern of surface silicate. This suggests that silicate regulates the diatom production, total new production, and thereby carbon cycle in this area. The modeled mean primary production is 48.4 mmolCm−2 day−1, representing the lower end of direct field measurements, while new production is 15.0 mmolCm−2 day−1, which compares well with previous estimates. 相似文献