Antioxidant and antitumor potential of wild and IMTA-cultivated Osmundea pinnatifida

Osmundea pinnatifida is a red edible seaweed known as pepper dulse. O. pinnatifida was cultivated in the farm of ALGAplus(Ilhavo, Portugal). This farm is integrated with a seabream and seabass commercial aquaculture and uses the nutrient-enriched water resultant from the fish production as its cultivation medium in the integrated multi-trophic aquaculture(IMTA) manner. Wild and IMTA-cultivated samples of O. pinnatifida were screened for antioxidant and antitumor activities. The antioxidant capacity of solvent extracts from wild and IMTA cultivated samples was assessed in two methods(2,2-diphenyl-1-picrylhydrazyl(DPPH) and oxygen radical absorbance capacity(ORAC)), and their total phenolic contents(TPC) were estimated. Antitumor activity was evaluated in three different tumor cell lines(HepG-2, MCF-7, and SH-SY5Y) through 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide(MTT)assay. Among the solvents used for extraction, dichloromethane was the most effective to extract phenolic compounds and presented higher ORAC. A significant correlation was found between TPC and ORAC,which was also sustained by the principal components analysis(PCA). Dichloromethane extracts induced a cytostatic effect on MCF-7 cells and showed weak cytotoxicity to SH-SY5Y cells and weak impact on cell proliferation. Overall, there were no statistically significant differences in the biological activities shown by the wild and IMTA-cultivated samples. Hence, O. pinnatifida can be obtained in an economical and environmentally sustainable way through IMTA, maintaining bioactive properties in a high potential for further nutraceutical purposes.     

Averaged model to study long-term dynamics of a probe about Mercury

This paper provides a method for finding initial conditions of frozen orbits for a probe around Mercury. Frozen orbits are those whose orbital elements remain constant on average. Thus, at the same point in each orbit, the satellite always passes at the same altitude. This is very interesting for scientific missions that require close inspection of any celestial body. The orbital dynamics of an artificial satellite about Mercury is governed by the potential attraction of the main body. Besides the Keplerian attraction, we consider the inhomogeneities of the potential of the central body. We include secondary terms of Mercury gravity field from \(J_2\) up to \(J_6\), and the tesseral harmonics \(\overline{C}_{22}\) that is of the same magnitude than zonal \(J_2\). In the case of science missions about Mercury, it is also important to consider third-body perturbation (Sun). Circular restricted three body problem can not be applied to Mercury–Sun system due to its non-negligible orbital eccentricity. Besides the harmonics coefficients of Mercury’s gravitational potential, and the Sun gravitational perturbation, our average model also includes Solar acceleration pressure. This simplified model captures the majority of the dynamics of low and high orbits about Mercury. In order to capture the dominant characteristics of the dynamics, short-period terms of the system are removed applying a double-averaging technique. This algorithm is a two-fold process which firstly averages over the period of the satellite, and secondly averages with respect to the period of the third body. This simplified Hamiltonian model is introduced in the Lagrange Planetary equations. Thus, frozen orbits are characterized by a surface depending on three variables: the orbital semimajor axis, eccentricity and inclination. We find frozen orbits for an average altitude of 400 and 1000 km, which are the predicted values for the BepiColombo mission. Finally, the paper delves into the orbital stability of frozen orbits and the temporal evolution of the eccentricity of these orbits.     

River-dominated and tide-influenced shelf-edge delta systems: Coarse-grained deltas straddling the Early–Middle Jurassic shelf–slope break and transforming downslope,Lajas–Los Molles formations,Neuquén Basin,Argentina

The three-dimensional facies and architecture variability of shelf-edge deltaic units cropping out at the transition between the Lower–Middle Jurassic Lajas and Los Molles formations of southern Neuquén Basin, Argentina, is presented here, as well as their stratigraphic relationship to uppermost deep-water slope channel systems. Deep-water, slope mudstones with thin turbidite beds merge upward with prodelta mudstones and thin sandstones, which are truncated by delta-front to mouth-bar sandstones. The latter sandstones are then downcut by large-scale, trough cross-stratified coarse-grained sandstones and conglomerates of distributary channel systems and along-strike, amalgamated with cross-bedded sandy units showing evidence of tidal reworking. Proximal–distal facies and architecture variability within a shelf-edge deltaic succession demonstrates that distributary channel-complexes become wider and deeper basinward, forming channelized river-dominated distributary fairways separated by tidally reworked inter-distributary sand belts at the shelf edge. Evidence from depositional-dip oriented outcrops shows a lack of collapsed and slumped strata at the shelf edge, and that the coarse shelf-edge distributary channel fills continue far down the deepwater slope, and conglomerates transform to become high-density turbidites to mainly thick-bedded, sand-matrix-supported debrites. The interplay between flood tides and river currents is interpreted to have primarily modulated the focusing of river drainages, and consequently coarse-grained sediment transport, along preferential routes on the outer-shelf to shelf-edge and down onto the slope. This contribution documents a unique example of coarse-grained (mostly conglomeratic) shelf-edge delta systems, tying bed-scale facies and architecture data to a seismic-scale shelf-margin morphology, thus providing outcrop analogue data for the characterization of shelf-edge delta systems in the subsurface.     

The influence of shrimp farms organic waste management on chemical water quality

We evaluated the physical–chemical properties and nutrient concentrations in two shrimp ponds under conventional management, intensive (I-P) and semi-intensive (SI-P), and one under organic management (O-P), three days after loading and before stoking. The mean’s total alkalinity concentrations were 2.26 ± 0.04 mM, 2.28 ± 0.01 mM and 2.59 ± 0.01 mM, respectively for I-P, SI-P and O-P. Bicarbonate and carbonate accounted for 62% and 37% in I-P, 64% and 36% in SI-P and 83–17% in O-P, respectively. Aragonite and calcite were oversaturated around Ω = 5. Mean total phosphate (TP) concentrations were 441.37 ± 92.06 μg/L, 449 ± 48 μg/L and 473.64 ± 84.17 μg/L, under I-P SI-P and O-P management respectively. Following this sequence, NO₃⁻ concentration was 2.98 ± 0.7 μg/L, 1.16 ± 0.16 μg/L and 0.32 ± 0.12 μg/L, under I-P, SI-P and O-P management respectively. Thus, the data suggest that different management of farm organic waste leads to different chemical water quality.     

HT-fabrics in a garnet-bearing quartzite from Western Portugal: geodynamic implications for the Iberian Variscan Belt

ABSTRACT The study of a garnet-bearing quartzite from a major suture zone in Iberia reports an unusual high-T fabric. Quartz c -axis patterns were plotted using shaped garnet as reference axis for the finite stretch ( X -axis). The pole figures are characterized by a dominant single maximum around X together with other point maxima along the XY plane (mylonitic foliation). These patterns suggest that dominant < c > prism slip and subordinated < a > prism slip operated during quartz plastic deformation in agreement with P–T conditions for syntectonic garnet growth (4–5 kbar and 700 ± 50 °C) and, pre-dating the well-known (late) Variscan D1 event (<6 kb and 600 ± 30 °C). The geotectonic framework suggests that the fabrics were formed along the western shear margin of the Ossa-Morena Zone during the early stages of the Variscan orogeny.     

Timing and duration of supergene mineralization at the Xinrong manganese deposit,western Guangdong Province,South China: cryptomelane <Superscript>40</Superscript>Ar/<Superscript>39</Superscript>Ar dating

Supergene Mn-oxide deposits are widely distributed in Guangxi, Guangdong, Yunnan, and Hunan Provinces, South China, accounting for 18% of the total Mn reserves in the country. Direct dating of supergene Mn enrichment, however, is lacking. In this paper, we present high-resolution ⁴⁰Ar/³⁹Ar ages of Mn oxides from the Xinrong Mn deposit, western Guangdong, to place numerical constraints on the timing and duration of supergene Mn enrichment. A total of ten cryptomelane samples, spanning a vertical extent of 67 m, were dated using the ⁴⁰Ar/³⁹Ar laser incremental heating technique, with seven samples yielding well-defined plateau or pseudo-plateau ages ranging from 23.48 ± 0.91 to 2.06 ± 0.05 Ma (2σ). One sample yields a staircase spectrum that does not reach a plateau; the spectrum, however, indicates the presence of two or more generations of Mn oxides in the sample, whose ages are best estimated at 22.34 ± 0.31 and 10.2 ± 0.86 Ma, respectively. The remaining two samples gave meaningless or uninterpretable results due to significant ³⁹Ar recoil and contamination by old phases. The ⁴⁰Ar/³⁹Ar data thus reveal a protracted history of weathering and supergene Mn enrichment that started at least in the end of the Oligocene or beginning of Miocene and extending into the latest Pliocene. Staircase-apparent age spectra, resulting from banded or botryoidal samples, yield an average growth rate of Mn oxides at 0.6–0.7 × 10⁻³ mm kyr⁻¹. The values indicate that a 1-mm grain of Mn oxides may host minerals precipitated during a time span of ca. 1.5 m.y., and accumulation of Mn oxides to form economic deposits under weathering environments may take millions of years. The distribution of weathering ages shows that the oldest Mn oxides occur on the top of the profile, whereas the youngest minerals are found at the bottom, suggesting downward propagation of weathering fronts. However, two samples located at the intermediate depths of the profile yield ages comparable with those occurring at the highest elevations. Such a complexity of age distribution is interpreted in terms of preferential penetration of Mn-rich weathering solutions along more permeable fault zones, or as a result of multi stages of dissolution and re-precipitation of Mn oxides. A synthesis of geochronological and geological data suggests that formation of the Xinrong deposit was a consequence of a combination of favorable lithological, climatic, and structural conditions. Because the climatic and structural conditions are similar among the provinces of South China during the Cenozoic, the geochronological results obtained at Xinrong may also have implications for the timing of supergene Mn enrichment throughout South China.     

A Matrix Approach Coupled with Monte Carlo Techniques for Solving the Net Radiative Balance of the Urban Block

A new method is developed for solving the shortwave and longwave net radiative balance of a three-dimensional urban structure, represented by parallelepiped blocks uniformly distributed in each direction. The method is based on a novel approach to determine the shape factors among surfaces, which are estimated by Monte Carlo techniques due to the complex geometry associated with the three-dimensional urban structure. Then, a set of linear equations is solved to quantify the radiative balance, in order to obtain their exact solution, considering all the inter-reflections among surfaces. The comparison between the new and the ray-tracing tracking methods resulted in a Pearson correlation coefficient of 0.996. However, by integrating the linear equations’ exact solution with Monte Carlo techniques, the new method reduces by a factor of 36 the central processing unit (CPU) time used to perform the calculations of the ray-tracing tracking method. The use of the model for a sensitivity study allows us to verify the effective absorptance and emittance increases with the canyon aspect ratio of the urban layout. An urban structure formed by square cross-sectional blocks absorbs more solar radiation than an urban structure formed by rectangular cross-sectional blocks. The approximation of a specific geometry for an equivalent bi-dimensional infinite street can be applied for rectangular cross-sectional blocks, where the width is 11 times or more greater than the depth dimension.     

Eutrophication processes and trophic interactions in a shallow estuary: Preliminary results based on stable isotope analysis (δ13C and δ15N)

Stable isotopes ratios (δ¹³C and δ¹⁵N) were measured in primary producers and consumers of two bays with contrasting eutrophic conditions in the Patos Lagoon estuary, southern Brazil: the Justino bay, a more pristine ecosystem, and the Mangueira bay, a heavily polluted region that receives the Rio Grande city sewage and effluencts of several industries. δ¹³C values of organisms collected in both subsystems were not different, but δ¹⁵N values had significant statistical differences, ca. 3.5‰ higher in the Mangueira bay. It is likely that primary producers and consumers in this subsystem are greatly influenced by higher nitrogen input due to domestic and industrial sewages. The stable isotope analysis also corroborated several trophic interactions previously established by gut content analysis, and due to its higher sensitivity, it was possible to better determine the contributions of different primary producers and detrital fractions to the consumers' diets. It was confirmed that plant detritus represents the main food source for most organisms. The stable isotope analysis also demonstrated that detritivorous benthic organisms in the same habitat have distinct diet compositions, with differential consumption of C3 and C4 plants. This technique showed that some consumers that eat detritus do not have in their stable isotopic signature any relationship with that of plants. It is likely that these consumers assimilate their carbon and nitrogen from other sources like microalgae or microorganisms that colonize decaying plants.