A method of batch-purifying microalgae with multiple antibiotics at extremely high concentrations

Axenic microalgal strains are highly valued in diverse microalgal studies and applications. Antibiotics, alone or in combination, are often used to avoid bacterial contamination during microalgal isolation and culture. In our preliminary trials, we found that many microalgae ceased growing in antibiotics at extremely high concentrations but could resume growth quickly when returned to an antibiotics-free liquid medium and formed colonies when spread on a solid medium. We developed a simple and highly efficient method of obtaining axenic microalgal cultures based on this observation. First, microalgal strains of different species or strains were treated with a mixture of ampicillin, gentamycin sulfate, kanamycin, neomycin and streptomycin (each at a concentration of 600 mg/L) for 3 days; they were then transferred to antibiotics-free medium for 5 days; and finally they were spread on solid f/2 media to allow algal colonies to form. With this method, five strains of Nannochloropsis sp. (Eustigmatophyceae), two strains of Cylindrotheca sp. (Bacillariophyceae), two strains of Tetraselmis sp. (Chlorodendrophyceae) and one strain of Amphikrikos sp. (Trebouxiophyceae) were purified successfully. The method shows promise for batch-purifying microalgal cultures.     

Hydrogen Incorporation in Crystalline Jadeite: Insight from First Principles Calculations

Hydrogen incorporation is critical for explaining defect energies, structure parameters and other physical characteristics of minerals and understanding mantle dynamics. This work analyzed the hydrogen complex defects in jadeite by the plane-wave pseudo-potential method based on density functional theory, and optimized the atomic positions and lattice constants in all configurations(different defective systems). Incorporation mechanisms considered for hydrogen(H) in jadeite include:(1) hydrogen incorporating with the O2 site oxygen and coexisting with M2 vacancy;(2) one H atom combined with an Al atom replacing Si in tetrahedron;(3) 4H atoms directly replacing Si in tetrahedron and(4) 3H atoms replacing Al on the M1 site. The four incorporation mechanisms mentioned above form the corresponding V_(Na)-H_i, Al_(Si)-H_i, V_(Si)-4H_i and V_(Al)-3H_i point defects. The molecular dynamics simulation to the ideal, V_(Na)-H_i, Al_(Si)-H_i, V_(Si)-4H_i and V_(Al)-3H_i point defects under the P-T conditions of 900 K, 2 GPa, the V_(Na)-H_i and Al_(Si)-H_i point defects under different pressures at T = 900 K, and Al_(Si)-H_i point defects under different temperatures at P = 3 GPa was performed to examine the preferential mode of hydrogen incorporation in jadeite by means of first-principles calculations. The calculations show that the averaged O–H bond-length in the hydrogen point defects system decreased in the order of Al_(Si)-H_i, V_(Na)-H_i, V_(Si)-4H_i and V_(Al)-3H_i. V_(Na)-H_i complex defects result in a contraction of the jadeite volume and the presence of Al_(Si)-H_i, V_(Si)-4H_i and V_(Al)-3H_i defects could increase the supercell volume, which is the most obvious in the V_(Al)-3H_i defects. The energy of formation of Al_(Si)-H_i and V_(Al)-3H_i complex defects was much lower than that of other defect systems. The V_(Al)-3H_i defects system has the lowest energy and the shortest O–H bond-length, suggesting that this system is the most favorable. The analytical results of vacancy formation energy, O–H bondlength, and the stability of the hydrogen defects in jadeite have suggested that the preferred hydration incorporation mode in jadeite is V_(Al)-3H_i complex defect.     

2016年6月23日江苏阜宁EF4级龙卷天气分析

2016年6月23日,江苏省盐城市阜宁县发生了历史罕见的EF4级龙卷,导致99人死亡,846人受伤,并有大量建筑物被损毁。文章利用观测资料对产生强龙卷的天气背景和中尺度特征进行了分析,发现：阜宁龙卷发生在我国东部龙卷最高发的地区和季节,产生龙卷的天气尺度背景为典型的梅雨期暴雨环流,产生龙卷的中尺度对流系统发生在地面暖锋南侧,这里也是高低空急流耦合的区域,与高空急流相伴的动力强迫特征明显,大气热力不稳定条件为中等偏强;产生阜宁龙卷的中尺度对流系统与美国大部分强龙卷相似,为块状的离散单体对流模态,且具有经典超级单体的钩状回波和强中气旋特征,并伴有龙卷涡旋特征（tornado vortex signature,TVS）;龙卷位于钩状回波顶端,主要发生在中气旋底高高度低于1 km期间。     

A Spatial Conditioned Latin Hypercube Sampling Method for Mapping Using Ancillary Data

For obtaining maps of good precision by the spatial inference method, the distribution of sampling sites in geographical and feature space is very important. For a regional variable with trends, the predicting error comes from trend estimation, variogram estimation and spatial interpolation. Based on the cLHS (conditioned Latin hypercube Sampling) method, a sampling method called scLHS (spatial cLHS) considering all these three aspects with the help of ancillary data is proposed in this article. Its advantage lies in simultaneously improving trend estimation, variogram estimation and spatial interpolation. MODIS data and simulated data were used as sampling fields to draw sample sets using scLHS, cLHS, cLHS with x and y coordinates as covariates, simple random and spatial even sampling methods, and the distribution and prediction errors of sample sets from different methods were evaluated. The results showed that scLHS performed well in balancing spreading in geographic and feature space, and can generate points pairs with small distances, and the sample sets drawn by scLHS produced smaller mapping error, especially when there were trends in the target variable.     

基于BP模型和Ward法的北京市平谷区乡村地域功能评价与分区

构建乡村地域功能评价指标体系,借助BP神经网络模型揭示平谷区“生产-生活-生态”功能分异特征;运用Ward法划分功能区,服务于平谷区乡村转型发展和功能区布局。结果表明：① 在多项功能综合作用下,平谷区基本形成了“平原地区生产功能较强、镇中心及周边村域生活功能明显、山地区以生态功能为主”的功能分布格局。② 基于评价结果和空间属性,平谷区划分为西部平原生产区、东部山地生态生产区、中部平原城市生活区、中部近郊平原生活区、东部丘陵生态生活区和北部山地生态区。其中,西部平原生产区、中部平原城市生活区、北部山地生态区是平谷三生空间功能发展的优势区域,分布相对集中。按照“强功能重优化、中等功能重提升、弱功能重改善”的原则提升乡村地域功能,促进地区均衡发展。     

Characteristics of mesoscale vortices over China in 2015

Mesoscale vortices, which appear at middle and lower levels of rainstorms, are cyclonic circulations with a size ranging from tens of kilometers to several hundred kilometers. Mesoscale vortices often have close relationships with convective activities. The ERA-Interim dataset and an automatic vortex-searching method were used to identify the mesoscale vortices occurring over China in 2015 and their basic characteristics were analyzed. The mesoscale vortices are divided into three categories: mesoscale convective vortices, mesoscale stratiform vortices, and mesoscale dry vortices. The mesoscale convective vortices have the largest intensity, size, and duration, whereas the mesoscale dry vortices have the smallest. Mesoscale convective vortices are able to form in any direction of the parent mesoscale convective system, although the secondary convection tends to appear to the southeast of the parent vortices. The mesoscale vortices tend to generate in the transition area between high and low altitudes. The leeward side of the Tibetan Plateau is the main source region of mesoscale vortices in China. Most of vortices are generated at midday and midnight. The activities of mesoscale convective vortices and mesoscale stratiform vortices peak in summer, whereas those of the mesoscale dry vortices peak in winter.     

一次输电线路覆冰舞动事故灾害性天气分析

利用WRF模式和Jones电线积冰模型对发生在2010年2月10—11日湖北地区的电线覆冰舞动事故的天气过程进行分析,并对灾害性天气的可预报性进行初步探讨。结果表明:Jones电线积冰模型基于观测降水数据计算积冰厚度在1.3~5.4mm,与观测量级相同。由于WRF模式对此次天气过程的降水量模拟偏大而高估了电线积冰厚度。WRF模式模拟的10m风速风向与观测的一致性较好,模拟本次过程的风速为5~10m·s-1,风向为偏北风,而湖北地区输电线路以东西走向为主,较容易发生舞动事故。     

T-E染色法用于对虾暴发性流行病的现场快速诊断

用Ｔ－Ｅ染色法可对该病进行现场快速诊断。取发病对虾的胃上皮区组织 或鳃组织，用Ｔ－Ｅ染液进行压片染色，可观察到上皮细胞核和结缔组织细胞核的病 毒性病理变化：细胞核质被挤到核的边沿，核内空泡化。发病严重的对虾胞核显著 膨大，核内呈嗜伊红着色。用这一染色法在现场仅需１０ｍｉｎ就可掌握对虾的细胞病 理变化，诊断结果与组织切片的Ｈ－Ｅ染色法一致。     

冬季东亚主要行星尺度环流分量的时空特征及其相互行用

本文采用一种复经验正交函数分析方法和滤波技术,对1978—79年冬季全球冬季风实验资料系列FGGE-Ⅲb进行分析,尤其对东亚地区时间尺度10—20天的主要行星尺度环流分量进行分解,结果表明影响气压和厚度场的决定性因子是冷季风潮,受季风潮影响200百帕上无辐散风u_φ第一模态以1—2天滞后沿经向和纬向有类似于季风潮的传播;季风潮振动导致局地经向环流相应变化,以无旋风V_X第一模态表示的Hadley环流几乎无经向传播但有明显的纬向传播,并在传播过程中加强;热带对于副热带存在反馈并通过u_φ第二模态实现。在急流入口处u_X第二模态表示次级环流的存在,其水平尺度约20个纬距。     

Long-Term Joint Distribution of One-Tenth Large (or Significant) Wave Height with Average Period

- In this paper, by using the wave data from a few oceanographic observation stations in the coastal zone of the Yellow Sea, the East China Sea and the South China Sea, the long-term joint distribution of the one-tenth large (or significant) wave height with average period is studied. The statistical data demonstrate that the long- term distribution of the one- tenth wave height or average period fits the log-normal distribution, thus the joint distribution also fits the two-dimensional log-normal distribution. Then the conditional probability distribution of the average period is derived, and the range as well as the mode of the average wave period corresponding to a certain return period of wave height can be calculated easily.