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261.
Perkins EJ Garcia-Reyero N Villeneuve DL Martinovic D Brasfield SM Blake LS Brodin JD Denslow ND Ankley GT 《Marine environmental research》2008,66(1):113-115
Ketoconazole is a fungicidal drug that inhibits function of cytochrome P450s in the synthesis of steroids. To examine if inhibition of P450 function affects gene expression in a dynamic manner, we conducted in vitro exposures of ovary tissue from fathead minnows (Pimephales promelas) to 0.5 microM ketoconazole to investigate effects on steroid production and gene expression over time. Expression of four key steroidogenesis genes was examined at 1, 6, and 12h of exposure. 11 beta- and 20 beta-hydroxysteroid dehydrogenases were down regulated at 1h and Cytochrome P450 17 was down-regulated at 12h, consistent with the absence of steroid production. In contrast, cytochrome P450 19A was up-regulated at 6h, indicating feedback regulation. Microarray analysis of 12h exposures indicated enrichment of biological processes involved in neurotransmitter secretion, lymphocyte cell activation, sodium ion transport, and embryonic development. These data suggest that, with the exception of cytochrome P450 19A, these steroid metabolic genes are regulated in a feed forward manner and that the effects of ketoconazole may be broader than anticipated based on the mechanism of action alone. 相似文献
262.
Matthew L. Keefer Christopher A. Peery Nancy Wright William R. Daigle Christopher C. Caudill Tami S. Clabough David W. Griffith Mark A. Zacharias 《Estuarine, Coastal and Shelf Science》2008
A common first step in conservation planning and resource management is to identify and classify habitat types, and this has led to a proliferation of habitat classification systems. Ideally, classifications should be scientifically and conceptually rigorous, with broad applicability across spatial and temporal scales. Successful systems will also be flexible and adaptable, with a framework and supporting lexicon accessible to users from a variety of disciplines and locations. A new, continental-scale classification system for coastal and marine habitats—the Coastal and Marine Ecological Classification Standard (CMECS)—is currently being developed for North America by NatureServe and the National Oceanic and Atmospheric Administration (NOAA). CMECS is a nested, hierarchical framework that applies a uniform set of rules and terminology across multiple habitat scales using a combination of oceanographic (e.g. salinity, temperature), physiographic (e.g. depth, substratum), and biological (e.g. community type) criteria. Estuaries are arguably the most difficult marine environments to classify due to large spatio-temporal variability resulting in rapidly shifting benthic and water column conditions. We simultaneously collected data at eleven subtidal sites in the Columbia River Estuary (CRE) in fall 2004 to evaluate whether the estuarine component of CMECS could adequately classify habitats across several scales for representative sites within the estuary spanning a range of conditions. Using outputs from an acoustic Doppler current profiler (ADCP), CTD (conductivity, temperature, depth) sensor, and PONAR (benthic dredge) we concluded that the CMECS hierarchy provided a spatially explicit framework in which to integrate multiple parameters to define macro-habitats at the 100 m2 to >1000 m2 scales, or across several tiers of the CMECS system. The classification's strengths lie in its nested, hierarchical structure and in the development of a standardized, yet flexible classification lexicon. The application of the CMECS to other estuaries in North America should therefore identify similar habitat types at similar scales as we identified in the CRE. We also suggest that the CMECS could be improved by refining classification thresholds to better reflect ecological processes, by direct integration of temporal variability, and by more explicitly linking physical and biological processes with habitat patterns. 相似文献
263.
264.
Understanding the dynamics and contamination of an urban aquifer system using groundwater age (14C, 3H,CFCs) and chemistry
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Jürgen Mahlknecht Arturo Hernández‐Antonio Christopher J. Eastoe Carol Tamez‐Meléndez Rogelio Ledesma‐Ruiz José A. Ramos‐Leal Nancy Ornelas‐Soto 《水文研究》2017,31(13):2365-2380
The quality of the groundwater supplying drinking water to the Guadalajara metropolitan area has deteriorated due to both endogenic and exogenic processes. Previous studies of this complex neotectonic volcanic environment suggest that the sources of contamination here are underground fluids derived from an active volcanic center and surface wastewater derived from regional land‐use intensification. This study uses isotopic, gaseous, and chemical signatures to more comprehensively characterize this groundwater flow and its contamination paths. Groundwater is mainly recharged at the La Primavera Caldera to the west and is discharged into the Santiago River to the east. The exception to this trend is the Toluquilla area, where groundwater most likely represents rainfall originating from outside the basin limits. Evaporation affects groundwater in these areas, especially waters that have been affected by recycling below urban areas in the Atejamac area and by intensive agricultural activity in the Toluquilla area. Additionally, we present evidence that groundwater flow through alluvial sediments and tuffs in deeper wells mixes with a lower aquifer unit in basaltic‐andesitic rocks, which are in contact with hydrothermal fluids. Groundwater ages range from postbomb in the western and northwestern regions of the study area (i.e., the Atemajac aquifer unit) to Late Pleistocene in the southern and southeastern regions (i.e., the Toluquilla aquifer unit). Recently recharged water records little mixing and is located mostly in or near the La Primavera volcanic system. As groundwater undergoes gravitational flow towards discharge areas, it mixes with older water components. Chloride and sodium concentrations above natural background levels are primarily related to volcanic activity, nitrate is associated with human activities, and sulfate originates from both anthropogenic sources and water–rock interactions. Nitrate originating from land‐use activities (such as sewers, septic tanks, landfills, and agricultural fields) that is introduced into the deeper part of the groundwater system is expected to travel with the groundwater to the discharge areas because oxidizing conditions will prevent microbial reduction. See Supplementary Information. 相似文献
265.
Distinguishing between debris flows and hyperconcentrated flows: an example from the eastern Swiss Alps
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Much research has been done on water‐rich mass flows, but the distinction between hyperconcentrated flows and debris flows, and whether the two are indeed different processes, continue to be debated. Here, we contribute to the ongoing discussion of these phenomena by describing and interpreting the deposit of a large landslide‐induced mass flow in the eastern Swiss Alps. About 9400 years ago, 10‐12 km3 of limestone detached from the wall of the Vorderrhein River valley and rapidly fragmented while sliding towards the valley bottom. The rock mass struck the valley floor with enormous force and liquefied at least 1 km3 of valley‐fill sediments. A slurry of liquefied sediment – the ‘Bonaduz gravel’ – traveled tens of kilometres down the Vorderrhein valley from the impact site, carrying huge fragments of rockslide debris that became stranded on the valley floor, forming hills termed ‘tomas’. Part of the flow was deflected by a cross‐valley barrier and traveled 14 km up a tributary of the Vorderrhein valley. The Bonaduz gravel is >65 m thick and fines upward from massive sandy cobble gravel at its base to silty sand at its top. Sedimentologic and geomorphic evidence indicates that Bonaduz gravel was transported as a hyperconcentated flow, likely above a basal carpet of coarse diamictic sediment that behaved as a debris flow. The large amount of water involved in the flow indicates that at least part of the Flims rockslide entered a lake. The Bonaduz deposit shares many properties with sediments left by hyperconcentrated flows generated in flumes, including normal grading and elutriation pipes produced by the rapid escape of fluids when the flow comes to rest. These properties are characteristic of non‐Newtonian laminar flows with high sediment concentrations. Our study reinforces laboratory and theoretical studies showing that debris flows and hyperconcentrated flows are different processes. © 2017 The Authors. Earth Surface Processes and Landforms published by John Wiley & Sons Ltd. 相似文献
266.
Samuel V. Panno Walton R. Kelly John Scott Wei Zheng Rachel E. McNeish Nancy Holm Timothy J. Hoellein Elizabeth L. Baranski 《Ground water》2019,57(2):189-196
Groundwater in karst aquifers constitutes about 25% of drinking water sources globally. Karst aquifers are open systems, susceptible to contamination by surface-borne pollutants. In this study, springs and wells from two karst aquifers in Illinois, USA, were found to contain microplastics and other anthropogenic contaminants. All microplastics were fibers, with a maximum concentration of 15.2 particles/L. The presence of microplastic was consistent with other parameters, including phosphate, chloride and triclosan, suggesting septic effluent as a source. More studies are needed on microplastic sources, abundance, and impacts on karst ecosystems. 相似文献
267.
After wildfire, hillslope and channel erosion produce large amounts of sediment and can contribute significantly to long-term erosion rates. However, pre-erosion high-resolution topographic data (e.g. lidar) is often not available and determining specific contributions from post-fire hillslope and channel erosion is challenging. The impact of post-fire erosion on landscape evolution is demonstrated with Structure from Motion (SfM) Multi-View Stereo (MVS) photogrammetry in a 1 km2 Idaho Batholith catchment burned in the 2016 Pioneer Fire. We use SfM-MVS to quantify post-fire erosion without detailed pre-erosion topography and hillslope transects to improve estimates of rill erosion at adequate spatial scales. Widespread rilling and channel erosion produced a runoff-generated debris-flow following modest precipitation in October 2016. We implemented unmanned aerial vehicle (UAV)-based SfM-MVS to derive a 5 cm resolution digital elevation model (DEM) of the channel scoured by debris-flow. In the absence of cm-resolution pre-erosion topography, a synthetic surface was defined by the debris-flow scour's geomorphic signature and we used a DEM of Difference (DoD) to map and quantify channel erosion. We found 3467 ± 422 m3 was eroded by debris-flow scour. Rill dimensions along hillslope transects and Monte Carlo simulation show rilling eroded ~1100 m3 of sediment and define a volume uncertainty of 29%. The total eroded volume (4600 ± 740 m3) we measured in our study catchment is partitioned into 75% channel erosion and 25% rill erosion, reinforcing the importance of catchment size on erosion process-dominance. The deposit volume from the 2016 event was 5700 ± 1140 m3, indicating ~60% contribution from post-fire channel erosion. Dating of charcoal fragments preserved in stratigraphy at the catchment outlet, and reconstructions of prior deposit volumes provide a record of Holocene fire-related debris-flows at this site; results suggest that episodic wildfire-driven erosion (~6 mm/year) dominate millennial-scale erosion (~5 mm/Ka) at this site. © 2019 John Wiley & Sons, Ltd. © 2019 John Wiley & Sons, Ltd. 相似文献
268.
This study assesses the effects of a high rise building on the beach and dune at an intensively developed shoreline through field investigation of geomorphological and sedimentological characteristics and wind tunnel investigation of wind speed and direction. Results indicate that wind speed is increased close to the building, where wind is often deflected to a more onshore direction. Obliquely offshore northwesterly winds are the most dramatically altered and cause onshore flows (with pronounced upward flows) in the landward portion of the beach near the building. Onshore deflections in wind direction cause besanding of the boardwalk and create highly localized scour and deposition zones. A dune on the backbeach breaks up zones of sediment transport and scour and diminishes the likelihood that scour areas will cause increased hazard to landward buildings as a result of flooding. Generic large-scale studies are required to develop criteria for communities to use to specify optimum dimensions, locations and shapes of landforms that are modified or created by high rise structures. 相似文献
269.
Nancy Romero-IbarraNorman Silverberg 《Continental Shelf Research》2011,31(16):1761-1776
The contents of 31 samples from free-drifting sediment traps deployed in the Gulf of St. Lawrence (GSL) were analyzed for the individual contribution of the different types of particles encountered to the total particulate organic carbon (POC) flux. Two trap models were used in 1993-1994: small traps at 50 m depth and large traps at 50 and 150 m. Total POC fluxes averaged 42 mg C m−2 d−1 for the more reliable large trap and 149 mg C m−2 d−1 for the small trap. The POC fluxes were attributed to different classes of particles based upon microscopically determined particle dimensions and carbon/volume algorithms available in the literature. Fecal pellets, followed by phytoplankton, were the major attributable components, with important contributions by microzooplankton, particularly during the summer of 1994. The mean fluxes for pellets (6 and 60 mg C m−2 d−1, for the large and small traps, respectively) and phytoplankton (3.2 and 42.9 mg C m−2 d−1) were in the range of those encountered in other areas of moderate primary productivity. Mean zooplankton carbon fluxes (1.8 and 8.5 mg C m−2 d−1, respectively), however, reflect higher than average zooplankton abundances in the GSL. The C fluxes of specific algal groups confirmed the existence of three trophic regimes previously identified from water column studies and numeric cell fluxes: (1) a period when diatoms were dominant during the spring, (2) a longer interval, which was dominated by dinoflagellates at most others times of the year, and (3) a period of transition during summer. Carbon of animal origin dominated the attributable flux, including an important fraction associated with heterotrophic dinoflagellates. The contribution of marine snow to the total flux (estimated as the difference between the total POC flux and the sum of the attributed components) frequently amounted to more than 60%. The true importance of marine snow remains uncertain, however, because the errors associated with each of the measured components accumulate to produce large uncertainties. The methodological problems involved are discussed. 相似文献
270.
Eliška Rejmánková Ji?í Komárek Margaret Dix Jaroslava Komárková Nancy Girón 《Limnologica》2011,41(4):296-302
Lake Atitlan, one of the most important lakes not only in Central America but in the whole world, is facing serious problems with increasing water pollution. Over the last several decades, the uncontrolled nutrient input into the lake has lead to high P levels and low N:P ratios, initiating cyanobacterial blooms. The first bloom occurred in December of 2008, followed by more extensive bloom in October 2009. The blooms are formed by cyanobacteria from the rare planktic Lyngbya hieronymusii/birgei/robusta complex. Based on the species morphology, the Atitlan population corresponds to L. robusta and this is the first case of reported bloom of this species worldwide. Remote sensing images documented that at the maximum bloom development, 40% of the 137 km2 of the lake area were covered by dense patches of Lyngbya, with the chlorophyll a concentration reaching over 100 μg L−1. The only toxins detected in the 2009 bloom were trace levels of cylindrospermopsin and saxitoxin with 12 and 58 ng g−1, respectively. The nitrogen fixation followed a pattern expected in non-heterocytous cyanobacteria, i.e., the nitrogenase activity was minimal during the day, while during the night the activity reached 2.2 nmol C2H4 μg Ch a−1 h−1. Delta 15N of −0.86‰ was well in the range given for nitrogen fixing organisms. The cell C, N and P content was 36.7%, 5.9% and 0.9%, respectively, resulting in the molar ratio of 105:14.4:1. A well designed and executed lake monitoring program, strict control of nutrient input into the lake, and public education are the necessary prerequisites for potential prevention of even more severe blooms than the one from 2009. 相似文献