Generation of reactive oxygen species in different fractions of the coelomocytes of holothurian Eupentacta fraudatrix in response to the thermostable toxin of Yersinia pseudotuberculosis in vitro

Pure fraction (92%–95%) of phagocytes (FP) and a mixture of amoebocytes (62%) and morula cells (38%)-FPMC- of the holothurianEupentacta fraudatrix' (Holothuroidea, Dendrochirota) were obtained by using ficoll-verographine step gradient. Basal production of reactive oxygen species (ROS) in FP quantified by using reduction of nitroblue tetrazolium (NBT) was more than twice that in FPMC. Thermostable toxin ofYersinia pseudotuberculosis (TST) at different concentrations (0.2; 0.5; 2.5 μg/ml, but not 0.1 μg/ml) stimulated NBT reduction in FPMC after 24 h incubation. In FP, TST at concentrations of 0.1 and 0.2 μg/ml inhibited and at concentrations of 0.5 and 2.5 μg/ml stimulated NBT reduction after 24 h incubation. Maximal effect was observed in FP and FPMC at TST concentrations of 0.5 and 0.2 μg/ml, respectively. Addition of catalase (0.7 μg/ml) to the cells treated with TST (2.5 μg/ml) was followed by a decrease in NBT reduction compared to that under toxin treatment alone. TST stimulated superoxide dismutase activity in concentration-dependent manner (maximum at 0.5 μg/ml concentration in FP) after 24 h treatment, and this stimulation was prevented by a commercial catalase. Plant lectin concanavalin A stimulated NBT reduction more than 5-fold in FPMC compared to the control. With addition of TST, lectin stimulated ROS to lesser extent than that with lectin alone. When catalase, TST, and lectin were added into the FPMC simultaneously, ROS increase was similar to that under lectin treatment alone. On the whole, data obtained indicated that ROS generation in holothurian coelomocytes especially occurs in both stimulated and not stimulated phagocytes, and that changes in ROS production by these cells may be one of the mechanisms of antibacterial protection of holothurians. This work was financially supported by the Russian Foundation for Fundamental Research Grant (No. 00-04-48949).     

Electric Discharges in the Protoplanetary Nebula as a Source of Impulse Magnetic Fields to Promote Dust Aggregation

Lightning discharge generated in the protoplanetary nebula is viewed as a temporally isolated surge in the flow of electrically charged particles, similar to that of terrestrial lightning. If the current is intense enough, a powerful circular impulse magnetic field is generated around the instantaneous virtual electric conductor. Such magnetic field is capable of magnetizing dust grains containing ferromagnetic components present in its vicinity to their saturation levels. As a result, dust grains attract one another, forming the aggregates. This magnetically driven attraction suggests an important process possibly operational at an early stage of the planetary accretion. Based on both a classical model for electric conductor, and the theory of Lienard–Wiechert electromagnetic potentials, our calculations show that the magnetic impulse due to a discharge channel of a few cm in diameter transferring a charge of about 10⁴ electrons reaches as high as 10 T. At these magnetic fields, the ferromagnetic dust grains, and possibly the already-formed larger aggregates as well, are easily magnetized to the saturation levels, producing compact clusters exhibiting permanent magnetic moments.     

Geometric Derivation of the Delaunay Variables and Geometric Phases

We derive the classical Delaunay variables by finding a suitable symmetry action of the three torus T³ on the phase space of the Kepler problem, computing its associated momentum map and using the geometry associated with this structure. A central feature in this derivation is the identification of the mean anomaly as the angle variable for a symplectic S ¹ action on the union of the non-degenerate elliptic Kepler orbits. This approach is geometrically more natural than traditional ones such as directly solving Hamilton–Jacobi equations, or employing the Lagrange bracket. As an application of the new derivation, we give a singularity free treatment of the averaged J ₂-dynamics (the effect of the bulge of the Earth) in the Cartesian coordinates by making use of the fact that the averaged J ₂-Hamiltonian is a collective Hamiltonian of the T³ momentum map. We also use this geometric structure to identify the drifts in satellite orbits due to the J ₂ effect as geometric phases.     

Cosmic structure growth and dark energy

Dark energy has a dramatic effect on the dynamics of the Universe, causing the recently discovered acceleration of the expansion. The dynamics are also central to the behaviour of the growth of large-scale structure, offering the possibility that observations of structure formation provide a sensitive probe of the cosmology and dark energy characteristics. In particular, dark energy with a time-varying equation of state can have an influence on structure formation stretching back well into the matter-dominated epoch. We analyse this impact, first calculating the linear perturbation results, including those for weak gravitational lensing. These dynamical models possess definite observable differences from constant equation of state models. Then we present a large-scale numerical simulation of structure formation, including the largest volume to date involving a time-varying equation of state. We find the halo mass function is well described by the Jenkins et al. mass function formula. We also show how to interpret modifications of the Friedmann equation in terms of a time-variable equation of state. The results presented here provide steps toward realistic computation of the effect of dark energy in cosmological probes involving large-scale structure, such as cluster counts, the Sunyaev–Zel'dovich effect or weak gravitational lensing.     

Robust Scalings in Compressible Flux Pile-Up Reconnection

Flux pile-up magnetic reconnection is traditionally considered only for incompressible plasmas. The question addressed in this paper is whether the pile-up scalings with resistivity are robust when plasma compressibility is taken into account. A simple analytical argument makes it possible to understand why the transition from a highly compressible limit to the incompressible one is difficult to discern in typical simulations spanning a few decades in resistivity. From a practical standpoint, however, flux pile-up reconnection in a compressible plasma can lead to anomalous electric resistivity in the current sheet and flare-like energy release of magnetic energy in the solar corona.     

Observations with the Visual and Infrared Mapping Spectrometer (VIMS) during Cassini's flyby of Jupiter

The Cassini Visual and Infrared Mapping Spectrometer (VIMS) is an imaging spectrometer covering the wavelength range 0.3-5.2 μm in 352 spectral channels, with a nominal instantaneous field of view of 0.5 mrad. The Cassini flyby of Jupiter represented a unique opportunity to accomplish two important goals: scientific observations of the jovian system and functional tests of the VIMS instrument under conditions similar to those expected to obtain during Cassini's 4-year tour of the saturnian system. Results acquired over a complete range of visual to near-infrared wavelengths from 0.3 to 5.2 μm are presented. First detections include methane fluorescence on Jupiter, a surprisingly high opposition surge on Europa, the first visual-near-IR spectra of Himalia and Jupiter's optically-thin ring system, and the first near-infrared observations of the rings over an extensive range of phase angles (0-120°). Similarities in the center-to-limb profiles of H⁺₃ and CH₄ emissions indicate that the H⁺₃ ionospheric density is solar-controlled outside of the auroral regions. The existence of jovian NH₃ absorption at 0.93 μm is confirmed. Himalia has a slightly reddish spectrum, an apparent absorption near 3 μm, and a geometric albedo of 0.06±0.01 at 2.2 μm (assuming an 85-km radius). If the 3-μm feature in Himalia's spectrum is eventually confirmed, it would be suggestive of the presence of water in some form, either free, bound, or incorporated in layer-lattice silicates. Finally, a mean ring-particle radius of 10 μm is found to be consistent with Mie-scattering models fit to VIMS near-infrared observations acquired over 0-120° phase angle.     

Pan-African eclogite facies metamorphism of ultramafic rocks in the Shackleton Range, Antarctica

In the Shackleton Range of East Antarctica, garnet-bearing ultramafic rocks occur as lenses in supracrustal high-grade gneisses. In the presence of olivine, garnet is an unmistakable indicator of eclogite facies metamorphic conditions. The eclogite facies assemblages are only present in ultramafic rocks, particularly in pyroxenites, whereas other lithologies – including metabasites – lack such assemblages. We conclude that under high-temperature conditions, pyroxenites preserve high-pressure assemblages better than isofacial metabasites, provided the pressure is high enough to stabilize garnet–olivine assemblages (i.e. ≥18–20 kbar). The Shackleton Range ultramafic rocks experienced a clockwise P–T path and peak conditions of 800–850 °C and 23–25 kbar. These conditions correspond to ∼70 km depth of burial and a metamorphic gradient of 11–12 °C km⁻¹ that is typical of a convergent plate-margin setting. The age of metamorphism is defined by two garnet–whole-rock Sm–Nd isochrons that give ages of 525 ± 5 and 520 ± 14 Ma corresponding to the time of the Pan-African orogeny. These results are evidence of a Pan-African suture zone within the northern Shackleton Range. This suture marks the site of a palaeo-subduction zone that likely continues to the Herbert Mountains, where ophiolitic rocks of Neoproterozoic age testify to an ocean basin that was closed during Pan-African collision. The garnet-bearing ultramafic rocks in the Shackleton Range are the first known example of eclogite facies metamorphism in Antarctica that is related to the collision of East and West Gondwana and the first example of Pan-African eclogite facies ultramafic rocks worldwide. Eclogites in the Lanterman Range of the Transantarctic Mountains formed during subduction of the palaeo-Pacific beneath the East Antarctic craton.     

Correlation between relative ages inferred from 26Al and bulk compositions of ferromagnesian chondrules in least equilibrated ordinary chondrites

Abstract— We have studied the relationship between bulk chemical compositions and relative formation ages inferred from the initial ²⁶Al/²⁷Al ratios for sixteen ferromagnesian chondrules in least equilibrated ordinary chondrites, Semarkona (LL3.0) and Bishunpur (LL3.1). The initial ²⁶Al/²⁷Al ratios of these chondrules were obtained by Kita et al. (2000) and Mostefaoui et al. (2002), corresponding to relative ages from 0.7 ± 0.2 to 2.4 ?0.4/+0.7 Myr after calcium‐aluminum‐rich inclusions (CAIs), by assuming a homogeneous distribution of ²⁶Al in the early solar system. The measured bulk compositions of the chondrules cover the compositional range of ferromagnesian chondrules reported in the literature and, thus, the chondrules in this study are regarded as representatives of ferromagnesian chondrules. The relative ages of the chondrules appear to correlate with bulk abundances of Si and the volatile elements (Na, K, Mn, and Cr), but there seems to exist no correlation of relative ages neither with Fe nor with refractory elements. Younger chondrules tend to be richer in Si and volatile elements. Our result supports the result of Mostefaoui et al. (2002) who suggested that pyroxene‐rich chondrules are younger than olivine‐rich ones. The correlation provides an important constraint on chondrule formation in the early solar system. It is explained by chondrule formation in an open system, where silicon and volatile elements evaporated from chondrule melts during chondrule formation and recondensed as chondrule precursors of the next generation.