Efficient detection of pathogen virus in sand dabs, Paralichthys olivaceus using loop-mediated isothermal amplification (LAMP)

Viral hemorrhagic septicemia virus (VHSV) and marine birnavirus (MABV) are the causative pathogens for some of the most explosive epidemics of emerging viral diseases in many Asian countries, leading to huge economic losses in aquaculture. Rapid molecular detection for surveillance or diagnosis has been a critical component in reducing the prevalence of pathogen infection. The loop-mediated isothermal amplification (LAMP) of DNA is currently one of the most commonly used molecular diagnostic tools, as it is simple, quick, and easy to amplify target DNA under isothermal conditions. In the present study, a novel and highly specific LAMP assay for the sensitive and rapid detection of VHSV and MABV infection in fish was developed. Using a set of synthesized primers matching a specific region of the genome, the efficiency and specificity of the LAMP assay were optimized in terms of the reaction temperature and DNA polymerase concentration, as they are the main determinants of the sensitivity and specificity of the LAMP assay. In particular, we demonstrated that our assay could be applied to efficient detection of VHSV and MABV infection in the wild fish, Paralichthys olivaceus. Our results demonstrate the simplicity and convenience of this method for the detection of viral infection in aquatic organisms.     

朝鲜平南盆地祥原超群的沉积时代与拉伸纪早期碳同位素负漂移

朝鲜平南盆地与东华北克拉通(中朝克拉通)中新元古代盆地的对比存在争议,准确厘定相关沉积建造的沉积时限是解决这些争议的关键。朝鲜平南盆地的地层主体为祥原超群,从下往上依次为直岘群、祠堂隅群、默川群、灭恶山群和燕滩群。直岘群是祥原超群的最下部层位,大部分由陆源碎屑岩组成。从平南盆地"北部型"和"南部型"直岘群碎屑岩中分选锆石,测得LA-ICP-MS U-Pb年龄,限定祥原超群沉积时代晚于1100Ma,并且得出所谓的"北部型"和"南部型"地层没有时代和物源差异。鉴于默川群的基性岩床时代为~900Ma,祥原超群的沉积时代为新元古代早期(1000~900Ma),燕滩群的时代可能稍晚。这说明平南盆地与华北徐淮盆地和旅大盆地等同时。祥原超群碳酸盐岩样品的δ~(13)C值数据表明,默川群时期碳同位素发生了负漂移(δ~(13)C值为-6‰~-5‰)。鉴于侵入到默川群的基性岩床的时代为~900Ma,我们认为该碳同位素(δ~(13)C值)负漂移略早于~900Ma,可能与同一时期的岩浆活动有关。     

Asian dust depositions over the Asian region during March 2010 estimated by ADAM2

The Asian Dust Aerosol Model 2 with the MM5 meteorological model has been employed to estimate the dust emission, dust concentration, and wet and dry deposition of dust in the Asian region for the month of March in 2010. It is found that the model simulates quite reasonably the dust (PM₁₀) concentrations both in the dust source region and the downstream region of Korea. The starting and ending times of most dust events and their peak concentration occurrence times are well simulated. The monthly mean maximum surface dust concentration (PM₁₀) is found to be 267???g?m^?3 in the domain of central northern China (CNC). Monthly total maximum dust emission of more than 32?t km^?2 and that of deposition of more than 25.4?t km^?2 (dry deposition of 24?t km^?2 and wet deposition of 1.4?t km^?2) are found to occur in the domain CNC, whereas the monthly mean minimum surface dust concentration (PM₁₀) is found to be 0.2???g?m^?3 in the domain of the Tibetan Plateau, where the monthly total dust emission (4?kg?km^?2) and the monthly total dust deposition (9?kg?km^?2) are found to be minimum. This monthly total dust deposition of 9?kg?km^?2 (dry deposition of 7?kg?km^?2 and wet deposition of 2?kg?km^?2) is as large as 2.25 times of that of emission (4?kg?km^?2), suggesting net dust influx toward the Tibetan Plateau from the surrounding dust source regions. It is also found that the ratio of the total dust deposition to the total dust emission in the source region increases toward the downstream direction from 0.4 in the upstream source region of Taklimakan to 0.80 in the downstream source region of northeastern China. More than 90% of the total dust deposition is found to be contributed by dry deposition due to the lack of precipitation in the dust source region. The monthly mean dust concentration (PM₁₀) is found to decrease with distance away from the dust source region. The monthly mean dust concentration of 62???g?m^?3 over the Yellow Sea (YES) decreases to 4.3???g?m^?3 over the Northwestern Pacific Ocean (NWP). The monthly total dust deposition in the downstream region is also found to decrease away from the source region from 2.33?t km^?2 (dry deposition of 1.36?t km^?2 and wet deposition of 0.97?t km^?2) over the domain YES to 1.45?t km^?2 (dry deposition of 0.16?t km^?2 and wet deposition of 1.30?t km^?2) over the domain NWP. A large amount of the total dust deposition over the seas is contributed by wet deposition (more than 90%), causing a small decreasing rate of the total dust deposition with distance from the source region. The estimated dust deposition could adversely impact the eco-environmental system significantly in the downstream regions of the Asian dust source region, especially over the seas.     

A simulation of Asian dust events observed from 20 to 29 December 2009 in Korea by using ADAM2

The Asian dust Aerosol Model 2 (ADAM2) with the MM5 meteorological model has been employed to study long-range transport process of Asian dust and to estimate dust emission, deposition (wet and dry) and concentration over the Asian dust source region and the downwind regions for dust events observed in Korea during the period of 20–29 December 2009, which is one of the dust events chosen by the 3^rd Meeting of Working Group for Joint Research on Dust Sand Storm among Mongolia, China, Japan and Korea to study intensively for the development of an early warning system in Asia. It is found that the model simulates quite well the starting and ending times of dust events and the peak dust concentrations with their occurrence times both in the source region and downwind regions. The dust emission in the dust source region is found to be associated with a developing synoptic weather system accompanied with strong surface winds over the source region that usually travels east to southeastward across the source region and then turns to move northeastward toward the north western Pacific Ocean. The dust emitted in the source region is found to be split into two parts: one is transported southeastward to the East China Sea in front of the surface high pressure system and experiencing enhanced deposition due to the sinking motion induced by the southeastward traveling the surface high pressure system whereas, the other moves northeastward toward the surface low pressure system and then lifted upward to form a upper-level high dust concentration layer that results in a favorable condition for the long-range transport of dust. It is also found that the maximum ten-day total dust emission of about 23 t km^?2 occurs in the domain Northwestern China (NWC). However, the maximum ten-day total dust deposition of 21 t km^?2 with the maximum mean surface concentration of 555 μg m^?3 and the column integrated mean concentration of 2.9 g m^?2 occurs in the domain Central-northern China (CNC). The column-integrated PM₁₀ concentration is found to increase toward northeastward especially in the domain North northeastern China (NNEC) due to the upper-level transported high PM₁₀ concentration. The ten-day total dust deposition, mean surface PM₁₀ and column integrated PM₁₀ concentrations in the downwind domains are found to decrease away from the source region from 2.44 t km^?2, 112 μg m^?3 and 1.68 g m^?2, respectively in the domain YES to 0.06 t km^?2, 2.1 μg m^?3 and 0.4 g m^?2, respectively in the domain Northwestern Pacific 1 (NWP1). Much of the total dust deposition is largely contributed by wet deposition in the far downwind region of the seas while that is contributed by dry deposition in the source region.     

朝鲜半岛冠帽地块的基底属性:来自银德洞变质侵入杂岩的锆石U-Pb年代学和岩石地球化学证据

地体基底建造的甄别和表征是联结华北克拉通与朝鲜半岛早期演化历史的基本纽带。针对朝鲜半岛前寒武纪基底建造研究方面的薄弱环节,本文表征了冠帽地块清津地区银德洞出露的变质深成侵入杂岩。3个代表性样品的SIMS锆石U-Pb定年结果显示,早期中性片麻岩记录的岩浆侵入年龄为约2.53 Ga,晚期钾长花岗质片麻岩记录的岩浆侵入年龄为约2.51 Ga,表明银德洞变质杂岩形成于新太古代晚期。参考变质杂岩的岩石地球化学特征,其原岩组成可能包括石英闪长岩和钾长花岗岩,大体上可与全球典型的岩浆型紫苏花岗岩系列相类比。结合3个样品变化较大的锆石εH f(t)值(-1.7~+10.5),我们推测银德洞变质侵入杂岩可能形成于玄武质下地壳岩石的部分熔融及其后的结晶分异作用,代表了活动大陆边缘岩浆弧环境下的典型产物。银德洞变质杂岩归属于新太古代岩浆弧根部岩浆岩建造的研究认识,不仅为判定冠帽地块与华北克拉通东部的吉南和龙地块以及辽北清原地块之间的亲缘性提供了岩石学证据,而且暗示这些地块在新太古代共同处在一个类似于安第斯活动大陆边缘的构造环境。