Optimization of Preparative Separation and Purification of Total PolyphenoJs from Sargassum tenerrimum by Column Chromatography

Polyphenols from the ethanol extracts of Sargassum tenerrimum （ST） with potent antiallergic effects were studied to optimize separation process through column chromatography. The adsorption and desorption characteristics of three widely used adsorbents： macroporous resin, silica gel, and polyvinylpolypyrrolidone （PVPP）, were critically evaluated respectively and studied for the optimization of preparative separation of polyphenols. Static operations on these adsorbents showed that macroporous resin had the best adsorption and desorption capability among the three adsorbents. Dynamic adsorption and desorption with macroporous resin packed column were also conducted to optimize the parameters such as： with the optimal values shown in brackets, the concen- tration of extract solution （4 times diluted）, pH value （6-7）, adsorption speed （3BVh-1, bed volumes/per hour）, concentration of ethanol （80%）, elution speed （3 BV ht） and elution volume （7 BV）. The chromatographic process so optimized gave a purity of 62.43% from the crude polyphenols, providing a promising basis for large scale preparation of bioactive polyphenols upon further scaling up tests.     

Measurement of wave-by-wave bed-levels in the swash zone

A technique is described to observe and quantify wave-by-wave bed-level changes in the swash zone. The ultrasonic instrument system is non-contact with the beach face surface being measured and the sensors remain outside of the fluid flows causing sediment movement. Sensor resolution combined with the electronic noise inherent within a digital network data-logging system results in a (conservative) measurement accuracy of ± 1 mm, equating to a couple of sand grain diameters in height. Illustrative field results demonstrate the practical use of the instrumentation, and a simple data pre-processing method to separate swashes and intervening bed-level ‘events’ is discussed. These example data reveal rather complex fluctuations of the bed observed over time periods of minutes to hours. Rather strikingly, gross bed-level changes per wave are revealed to be up to many orders of magnitude larger than the observed net rate of beach face evolution. It is outlined how observations of successive bed-level changes at multiple locations within a dense grid, combined with a consideration of sediment continuity, will now enable the total net sediment transported per uprush–backwash to be quantified.     

iShocks: X-ray binary jets with an internal shocks model

In the following paper, we present an internal shocks model, iShocks, for simulating a variety of relativistic jet scenarios; these scenarios can range from a single ejection event to an almost continuous jet, and are highly user configurable. Although the primary focus in the following paper is black hole X-ray binary jets, the model is scale and source independent and could be used for supermassive black holes in active galactic nuclei or other flows such as jets from neutron stars. Discrete packets of plasma (or 'shells') are used to simulate the jet volume. A two-shell collision gives rise to an internal shock, which acts as an electron re-energization mechanism. Using a pseudo-random distribution of the shell properties, the results show how for the first time it is possible to reproduce a flat/inverted spectrum (associated with compact radio jets) in a conical jet whilst taking the adiabatic energy losses into account. Previous models have shown that electron re-acceleration is essential in order to obtain a flat spectrum from an adiabatic conical jet: multiple internal shocks prove to be efficient in providing this re-energization. We also show how the high-frequency turnover/break in the spectrum is correlated with the jet power,  ν _b ∝ L ^∼0.6_W  , and the flat-spectrum synchrotron flux is correlated with the total jet power,   F _ν∝ L ^∼1.4_W  . Both the correlations are in agreement with previous analytical predictions.     

Effects of Dietary Protein Level on Growth and Utilization of Protein and Energy by Juvenile Mangrove Red Snapper （ Lutjanus argentimaculatus ）

A feeding trial was conducted in a recirculating water system to investigate the effects of dietary protein levels on growth, feed utilization, hepatosomatic index and liver lipid deposition of juvenile red snapper, Lutjanus argentimaculatus (average initial wet weight 8.0 ± 0.39 g and total length 3.14 ± 0.3 cm). In the experiment, six fishmeal-based diets were formulated to contain various protein levels (20% to 45% in 5% increments), with dietary energy ranging from 2210.7kJ lOOg to 2250.2kJlOOg dry matter. The protein to energy ratios of diets ranged from 8.58 mg protein kJ⁻¹ to 20.03 mg protein kJ⁻¹. Diets were fed for 90d to triplicate groups of fish stocked in 0.128m³ seawater tanks, 25 individuals each. The daily ration of 2% wet body weight was offered to the fish thrice a day. The fish at the end of the study had more than ten-fold (77.0g) increase in weight compared to the initial (8.0g). Fish fed diets of 40% and 45% protein produced significantly (P<0.05) higher weight gain of 77.2g and 76.5g, and specific growth rate (SGR) of 2.65% and 2.62% than those of 67.0 g and 68.3g, and 2.49% and 2.51% of the other diets. The broken-line regression of SGR against dietary protein level yielded an optimum dietary protein requirement of 42.6% (Y=−1.6295 + 0.1114 X ²,P<0.05). Survival remained 100% among groups. Feed conversion ratio decreased from 0.45 for fish fed 20% dietary protein to 0.35 for fish fed 45% dietary protein. Nitrogen intake increased with an increase in dietary protein, which in turn resulted in an increase in nitrogen gain of fish whole body. Fish fed 40% and 45% protein diets showed higher (P<0.05) nitrogen gain (0.27g and 0.26g) than those (0.23g and 025g) fed all other diets. Gross energy intake (GEI) in fish fed 45% protein was lower (600.67kJ) than that (607.97 kJ) of 40% protein diet, though the differences were not statistically significant (P>0.05); GEI ranging from 677.31 kJ to 663.20 kJ at remaining four diets (20% to 35% protein) did not appear to differ significantly (P>0.05). The highest energy gain of 518.33 kJ was obtained with fish fed 40% protein, resulting in the highest energy retention efficiency of 85.26%. The hepatosomatic index of fish fed diets of 20%, 25%, 30% and 35% protein were significantly (P<0.05) higher (2.09% to 2.57%) than those (1.44% and 1.41%) of fish fed diets containing 40% and 45% protein. Liver lipid contents decreased from 8.72% to 7.0% in fish fed dietary protein of 20% to 45% in 5% increments. Results suggest that the diet containing 40% to 42.6% protein with a P/E ratio of 17.6 mg protein kJ⁻¹ is required for good growth of L. argentimaculatus weighing between 8.0 g and 85.2 g under the culture conditions of the present study.     

Latitude effects in lunar thermal evolution

A simple analytical model is developed from which we have calculated the temperature throughout the lunar interior resulting from internal heat sources and the imposition of surface temperature boundary conditions. The surface temperature is determined almost entirely by the balance of solar heating and surface reradiation; as a consequence this temperature is latitude dependent, decreasing towards the lunar poles. The internal solution shows that the latitude effect exists almost undiminished to great depths within the Moon. It is suggested that this dependence on latitude may have a significant effect on the Moon’s thermal evolution. Using the liquefaction model the high concentration of lunar maria at low latitudes may be explained.     

Thermodynamics of phantom energy in the presence of a Reissner-Nordström black hole

In this paper, we study the validity of the generalized second law (GSL) in phantom dominated universe in the presence of a Reissner-Nordström (RN) black hole. Our study is independent of the origin of the phantom like behavior of the considered universe. We also discuss the GSL in the neighborhood of transition from quintessence to phantom regime. We show that for a constant equation of state parameter, the GSL may be satisfied provided that the temperature is proportional to de Sitter temperature. It is shown that in models with (only) a transition from quintessence to phantom regime the generalized second law does not hold in the transition epoch. Next we show that if the phantom energy has a chemical potential, then the GSL will hold if the mass of black hole is above from a critical value.     

FRW and Bianchi type I cosmology of f-essence

F-essence is a generalization of the usual Dirac model with the nonstandard kinetic term. In this paper, we introduce a new model of spinor cosmology containing both Ricci scalar and the non minimally coupled spinor fields in its action. We have investigated the cosmology with both isotropy and anisotropy, where the equations of motion of FRW and Bianchi type-I spacetimes have been derived and solved numerically. Finally the quantization of these models through Wheeler-De Witt (WD) wave function has been discussed.     

Improved method of generation of artificial time-histories,rich in all frequencies,from floor spectra

A method to generate artificial time-histories from given floor response spectra is presented in this paper. The original method proposed by Levy and Wilkinson¹ is modified. New iterative equations are developed, based on extensive computational experimentation, to speed up and stabilize the convergence process. The present approach, when applied to spectra with peaks in the high frequency range (40 to 100 Hz) along with seismic peaks, converges very closely to the target spectra within eight iterations regardless of damping values. The original computer program for synthetic time-history generation was developed on the UNIVAC 1100 series computer. This program was converted to work on the IBM PC. The PC based program ‘MICRO-SEIM’ saves the response spectra generated at each iteration and the final synthetic time-history on the PC diskette. The post processing program reads those saved data from the diskette and plots the spectra and time-history.