Optimization of preparative separation and purification of total polyphenols from Sargassum tenerrimum by column chromatography

Polyphenols from the ethanol extracts of Sargassum tenerrimum (ST) with potent antiallergic effects were studied to optimize separation process through column chromatography. The adsorption and desorption characteristics of three widely used adsorbents: macroporous resin, silica gel, and polyvinylpolypyrrolidone (PVPP), were critically evaluated respectively and studied for the optimization of preparative separation of polyphenols. Static operations on these adsorbents showed that macroporous resin had the best adsorption and desorption capability among the three adsorbents. Dynamic adsorption and desorption with macroporous resin packed column were also conducted to optimize the parameters such as: with the optimal values shown in brackets, the concentration of extract solution (4 times diluted), pH value (6–7), adsorption speed (3 BV h⁻¹, bed volumes/per hour), concentration of ethanol (80%), elution speed (3 BV h⁻¹) and elution volume (7 BV). The chromatographic process so optimized gave a purity of 62.43% from the crude polyphenols, providing a promising basis for large scale preparation of bioactive polyphenols upon further scaling up tests.     

Measurement of wave-by-wave bed-levels in the swash zone

A technique is described to observe and quantify wave-by-wave bed-level changes in the swash zone. The ultrasonic instrument system is non-contact with the beach face surface being measured and the sensors remain outside of the fluid flows causing sediment movement. Sensor resolution combined with the electronic noise inherent within a digital network data-logging system results in a (conservative) measurement accuracy of ± 1 mm, equating to a couple of sand grain diameters in height. Illustrative field results demonstrate the practical use of the instrumentation, and a simple data pre-processing method to separate swashes and intervening bed-level ‘events’ is discussed. These example data reveal rather complex fluctuations of the bed observed over time periods of minutes to hours. Rather strikingly, gross bed-level changes per wave are revealed to be up to many orders of magnitude larger than the observed net rate of beach face evolution. It is outlined how observations of successive bed-level changes at multiple locations within a dense grid, combined with a consideration of sediment continuity, will now enable the total net sediment transported per uprush–backwash to be quantified.     

In-situ estimates of net sediment flux per swash: Reply to discussion by TE Baldock of “Measurement of wave-by-wave bed-levels in the swash zone”

This paper replies to TE Baldock's discussion [Coastal Eng. 56 (2009) 380–381] of ‘Measurement of wave-by-wave bed-levels in the swash zone’ by Turner et al. [Coastal Eng. 55 (2008) 1237–1242]. We address and extend the comparison and discussion of ultrasonic bed-level sensors and buried pressure transducers to obtain estimates of the beach face elevation within the swash zone. We demonstrate the use of the former method to obtain many and continuous (every time the beach face is exposed) in-situ estimates of net sediment flux per swash.     

Evaluation of SEBS for estimation of actual evapotranspiration using ASTER satellite data for irrigation areas of Australia

Spatial knowledge of land surface evapotranspiration (ET) is of prime interest for environmental applications, such as optimizing irrigation water use, irrigation system performance, crop water deficit, drought mitigation strategies, and accurate initialization of climate prediction models especially in arid and semiarid catchments where water shortage is a critical problem. The recent drought in Australia and concerns about climate change have highlighted the need to manage water resources more sustainably especially in the Murrumbidgee catchment which utilizes bulk water for food production. This study deals with the application of a Surface Energy Balance System (SEBS) algorithm based on Advanced Spaceborne Thermal Emission and Reflection radiometer (ASTER) data and field observations has been proposed and tested for deriving ET over Coleambally Irrigation Area, located in the southwest of NSW, Australia. We have used 12 ASTER scenes covering the time period of 2002, 2003, 2004, 2005, 2006, and 2009 for estimating the actual ET over the study area. To validate the proposed methodology, the ground-measured ET was compared to the ASTER-derived actual ET values for the study area. The derived ET value over the study area is much closer to the field measurement. From the remote sensing results and observations, the root mean square error is 0.89 and the mean absolute percentage difference is 2.87 %, which demonstrate the reasonability of SEBS ET estimation for the study area.     

Geology,geochemistry and mineralogy of the lignite-hosted Ambassador palaeochannel uranium and multi-element deposit,Gunbarrel Basin,Western Australia

The Ambassador U and multi-element deposit occurs on the SW margin of the Gunbarrel Basin, Western Australia. Low-grade, flat-lying U mineralization averaging about 2 m thick at 0.03% U occurs in lignites at the redox front at the base of the weathering profile within a laterally extensive palaeochannel network. Uranium is principally associated with organic matter within the lignitic matrix, although rare discrete U minerals, such as coffinite and uraninite, are also present. The lignite is also enriched in a suite of other elements, principally base metals and sulphur, with concentrations of 0.3 ≥ 1% Cu, Pb, Ni, Co, Zn and total rare earth elements (REE) in some samples. Other element enrichments include: Cr, Cs, Sc, Se, Ta, Ti, Th, V and Zr as detrital heavy minerals of Zr, Ti and REE (oxides and silicates) or authigenic minerals of Cu, Bi, Pb, Zn, Ni, Se, Hg, Ti, Cr, Tl, V, U and REE (sulphides, vanadates, selenides, oxides, chlorides and native metals) and diffuse lignite impregnations. The Ambassador deposit probably formed from the convergence of redox-active weathering processes to unique source/host rocks, constrained within the palaeochannel. A proximal source of U and trace elements of lamproite/carbonatite origin is probable, as constrained by U–Pb isotope and U–Th disequilibria studies. Uranium and other metals were precipitated syngenetically with organic matter as it was deposited during a humid phase in the Late Eocene. Remobilization subsequently concentrated the metals in the upper 2 m of the lignite. This may have occurred during one or more periods of weathering and associated diagenesis, with the latest episode in the last 300,000 years.     

Seasonal variations in the isotopic composition of leaf and stem water from an arid region of Southeast Asia

Abstract

Stable isotopes are powerful research tools in environmental sciences and their use in ecosystem research is increasing. Stable isotope measurements allow the study of evapotranspiration fluxes, soil evaporation and leaf transpiration phenomena. Soil water and leaf water are the sources of the evapotranspiration that transfers large quantities of water from land to the atmosphere; as a result the isotopic composition of water left in the leaves is modified towards enrichment. Evaporation also changes the isotopic composition of water bodies creating a natural isotopic signal. The isotopic identity of soil water affects the oxygen isotopic signature of leaf and stem water. In this paper we present the isotopic data of bulk leaf water, showing the enrichment in isotopic value of oxygen due to evapotranspiration from leaves in conjunction with the isotopic signal of rainwater and other environmental factors such as humidity and temperature. Results suggest that the variation in the values of δ¹⁸O of Eucalyptus citriodora, Dalbergia sissoo, Melia azedarach and Pinus roxburghii is due to the seasonal changes in the δ¹⁸O of the source water for plants, i. e. rain. It is further observed that leaf water δ¹⁸O values are depleted during the months of July, August and September. This occurs due to the following reasons: (a) the sampling areas receive about 50% of the average annual rain during these months, and (b) rainfalls during these months are isotopically depleted compared with winter rains.

Citation Butt, S., Ali, M., Fazil, M. & Latif, Z. (2010) Seasonal variations in the isotopic composition of leaf and stem water from an arid region of Southeast Asia. Hydrol. Sci. J. 55(5), 844–848.     

On role of pressure anisotropy for relativistic stars admitting conformal motion

We investigate the spacetime of anisotropic stars admitting conformal motion. The Einstein field equations are solved using different ansatz of the surface tension. In this investigation, we study two cases in details with the anisotropy as: (1) p _t =n p _r , (2) -p_r=(+c_2)p_{t}-p_{r}=\frac{1}{8\pi}(\frac{c_{1}}{r^{2}}+c_{2}) where, n, c ₁ and c ₂ are arbitrary constants. The solutions yield expressions of the physical quantities like pressure gradients and the mass.     

Fishery stock assessment of Kiddi shrimp (Parapenaeopsis stylifera) in the Northern Arabian Sea Coast of Pakistan by using surplus production models

Pakistani marine waters are under an open access regime.Due to poor management and policy implications,blind fishing is continued which may result in ecological as well as economic losses.Thus,it is of utmost importance to estimate fishery resources before harvesting.In this study,catch and effort data,1996-2009,of Kiddi shrimp Parapenaeopsis stylifera fishery from Pakistani marine waters was analyzed by using specialized fishery software in order to know fishery stock status of this commercially important shrimp.Maximum,minimum and average capture production of P.stylifera was observed as 15 912 metric tons(mt)(1997),9 438 mt(2009) and 11 667 mt/a.Two stock assessment tools viz.CEDA(catch and effort data analysis) and ASPIC(a stock production model incorporating covariates) were used to compute MSY(maximum sustainable yield) of this organism.In CEDA,three surplus production models,Fox,Schaefer and Pella-Tomlinson,along with three error assumptions,log,log normal and gamma,were used.For initial proportion(IP) 0.8,the Fox model computed MSY as 6 858 mt(CV=0.204,R~2=0.709) and 7 384 mt(CV=0.149,R~2=0.72) for log and log normal error assumption respectively.Here,gamma error produced minimization failure.Estimated MSY by using Schaefer and Pella-Tomlinson models remained the same for log,log normal and gamma error assumptions i.e.7 083 mt,8 209 mt and 7 242 mt correspondingly.The Schafer results showed highest goodness of fit R~2(0.712) values.ASPIC computed MSY,CV,R~2,F_(MSY)and B_(MSY) parameters for the Fox model as 7 219 mt,0.142,0.872,0.111 and 65 280,while for the Logistic model the computed values remained 7 720 mt,0.148,0.868,0.107 and 72 110 correspondingly.Results obtained have shown that P.stylifera has been overexploited.Immediate steps are needed to conserve this fishery resource for the future and research on other species of commercial importance is urgently needed.