Identification and characterization of the Vibrio anguillarum prtV gene encoding a new metalloprotease

We cloned and sequenced a prtV-like gene from Vibrio anguillarum M3 strain. This prtV gene encodes a putative protein of 918 amino acids, and is highly homologous to the V. cholerae prtV gene. We found that a prtV insertion mutant strain displayed lower gelatinase activity on gelatin agar, lower protease activity against azocasein, and lower activity for four glycosidases. This prtV mutant strain also had increased activity for two esterases in its extracellular products, as analyzed by the API ZYM system. In addition, the prtV mutant strain exhibited decreased growth in turbot intestinal mucus and reduced hemolytic activity on turbot erythrocytes. Infection experiments showed that the LD₅₀ of the prtV mutant strain increased by at least 1 log compared to the wild-type in turbot fish. We propose that prtV plays an important role in the pathogenesis of V. anguillarum.     

非均匀温度场中熔体结晶作用与科马提岩结构成因

具有重要岩石学和构造学意义的科马提岩，最吸引人同时又最令人困惑的特征之一是它的鬣刺结构和分层构造。本文从喷出地表的岩桨冷凝时温度分布、熔体性质与结晶作用的密切联系，把科马提岩浆喷发形成的岩流作为一个冷凝整体。通过与金属铸锭浇注成形和矿石冶炼生产的比较，发现它们在冷凝固结过程中温度场特征、熔体的性质、结晶能力和结晶方式都有十分相似的特点，并以此为出发点详细解析了科马提岩的鬣刺结构和岩石分层构造的形成     

网络环境下三维模型的多尺度数据组织方法

本文针对网络环境下海量三维模型数据的传输与可视化要求,探讨了三维模型数据多尺度组织方法.设计了基于点索引的三维模型几何数据结构,结合多尺度纹理表达,构建三维模型多尺度数据结构.在此基础上,基于四叉树结构构建分层分块的三维模型数据多尺度存储结构,从而适应三维模型数据的多尺度分块调度与传输,并通过实验验证该方法能根据三维场...     

Characteristics of Sulfide Removal by Hydrogenovibrio thermophilus Strain TT in Mariculture System

To biologically inhibit the production of highly toxic sulfide in the aquaculture area, one bacterium, designated as Hydrogenovibrio thermophilus strain TT, was isolated from sediment in Jiaozhou Bay, China. The strain was found to be microaerobic mixtrophic, employing both carbon dioxide and organic carbons, e.g. citrate, lactate, glucose, and peptone as carbon sources. When it oxidized the sulfide and thiosulfate, oxygen was the optimal electron acceptor, followed by nitrite and nitrate, which benefited to removal of sulfide under low-oxygen mariculture environment. In addition, the strain TT exhibited remarkable tolerance of sulfide and thiosulfate in seawater, and it was able to oxidize 18.52 mmol L~(-1) S~(2-) in 12 h or 45 mmol L~(-1) S_2O_3~(2-) in 24 h at initial pH 7.0-9.0, 30-40℃. Therefore, this strain showed a quite promising application for biological remediation of sulfide-contaminated mariculture system.     

深圳市地理信息产业政策研究及建议

深圳市2014年地理信息产业调查估算产值为50-60亿元,产业发展潜力具有很大的挖掘空间。有效的产业政策可以促进产业发展。为加快深圳地理信息产业发展,本文在对深圳市现有地理信息产业政策体系进行全面梳理和政策问题分析的基础上,开展促进深圳市地理信息发展的产业政策研究,提出深圳市地理信息产业优先发展方向、领域,并基于地理信息产业政策研究理论框架,给出具有针对性的政策完善建议。     

基于点云数据内插DEM的精度比较研究

利用3维激光扫描技术,以云南元谋干热河谷地区冲沟为研究对象,扫描获得大量具有冲沟坐标信息的点云数据。离散的点云数据之间没有明显的拓扑关系,不能表达连续的地表形态,通过建立格网DEM可视化表达地形信息,利用不同的内插方法生成DEM并进行精度评定,以便在不同的地貌区和采点方式下选用最佳的内插模型。点云数据经过数据配准拼接以及噪声剔除,在ArcGIS软件中,通过数据格式转换,利用反距离加权插值、克里金插值、自然领域法插值三种不同的内插方法生成DEM模型,通过检查点法分析已有的固定点高程和内插相应点的高程值做误差比较,分析不同的内插精度。实验表明,反距离加权插值法和克里金插值中误差基本相同,自然领域法插值中误差最大。分析比较不同的内插精度,对地表的坡度、坡向、等高线的研究具有重要的意义。     

Geochemical Studies of Sodium Enriched Metavolcanite Series in the Area of Longbohe Copper Deposit,Yunnan Province,SW China：Nd,Sr and Pb Isotopic Characteristics and Chronology

The chronological data obtained by a variety of dating methods have confirmed that the sodium enriched metavolcanite series in the area of the Longbohe copper deposit at Jinping, Yunnan Province, was formed during the Proterozoic (its Pb−Pb isochron age=1595±75 Ma), corresponding to the Dahongshan Group sodium enriched metavolcanites. The Sm−Nd isochron age of 1336±46Ma should represent the time at which this series of volcanic rocks experienced spilitization in response to sodium metasomatism, equivalent to the time of sedimentation of the Ailaoshan Group and Yashan Group rocks. The Rb−Sr ages of the volcanic rocks and the Pb−Pb ages of copper ores have recorded the events of strong dynamic metamorphism and of strong reworking-metallogenesis of copper during the Jinningian period. Similarities in forming age, rock assemblage and isotopic characteristics between the sodium enriched metavolcanite series and the Dahongshan Group sodium enriched metavolcanite series have provided new clues to the exploration of the Dahongshan-type copper deposits in this area. Granted jointly by the National Natural Science Foundation of China (Nos. 49702022, 40073001) and the State 973 Program (No. G1999043215).     

我国陆上大中型气田分布规律与有利勘探区带优选

“八五”、“九五”期间我国天然气探明储量幅度增长,大中型气田分布规律的深化研究密切关系到天然气储量的增长势头。克拉通盆地下层序古隆起控气理论和上层序非构造圈闭控气机理是克拉通大中型气田富集分布的基本规律,前陆盆地是我国富气盆地类型之一,其冲断带下盘大型造圈闭群、前缘斜坡岩性尖灭区带与浅层次生气藏、前缘隆起上的断块圈闭群等是该类盆地有利的天然气富集区带。依据充分的地质资料,结合我国天然气勘探实际,提