松江鲈(Trachidermus fasciatus)泛素结合酶E2-D2基因的分子克隆及组织表达分析

泛素结合酶(UbcE2)是蛋白泛素化过程中所必需的酶,在泛素转移和底物的特异性识别方面发挥着重要的作用。本实验利用RACE技术克隆获得了全长为993bp的松江鲈泛素结合酶E2-D2基因cDNA序列(命名为TfUbcE2-D2),其开放阅读框为444bp,编码147个氨基酸。通过SMART预测得知,TfUbcE2-D2含有一个UBCc结构域。同源比对结果表明该基因与其他物种的同源性为92.31%。实时荧光定量PCR显示,TfUbcE2-D2广泛表达于松江鲈各组织,在肾脏中的相对表达量最高,其次为鳃。鳗弧菌(Vibrio anguillarum)刺激后,TfUbcE2-D2在血液、脾脏、肝脏和鳃中表达均上调,其中,脾脏和鳃中的表达量上调约40倍。由以上实验结果推测,TfUbcE2-D2可能参与松江鲈的先天免疫防御。     

鄂尔多斯地区晚古生代沉积体系及古地理演化

采用新的地层划分方案,在野外露头、钻井剖面的综合研究和室内编图基础上,提出陆表海碳酸盐台地-碎屑堡岛-浅水三角洲复合沉积体系是鄂尔多斯地区晚古生代重要的古地理格局。受区域构造背景影响,晚古生代盆地充填经历了裂陷海湾和陆表海浅陷共存、统一陆表海、近海内陆坳陷和内陆湖盆四个阶段,区域古地理格局由陆表海碳酸盐台地-碎屑堡岛-浅水三角洲复合体系沉积演变为三角洲-河流体系沉积,最终被河流-湖泊体系沉积取代。砂质储集体发育受控于沉积环境,主要展布在中、北部地区,以河流、三角洲砂体为主。     

Three-dimensional numerical experiments on tidal exchange through a narrow strait in a homogeneous and a stratified sea

We have investigated the three-dimensional Lagrangian motion of water particles related with tidal exchange between two basins with a constant depth connected through a narrow strait and the effects of density stratification on the exchange processes by tracking a number of the labeled particles. Tide-induced transient eddies (TITEs), which are similar to those in two-dimensional basin, are generated behind the headlands. Upwelling appears around the center of the eddy and sinking around the boundary. When the basins are filled with homogeneous water, a pair of vortices are produced in the vertical cross section of the strait due to bottom stress, with upwellings along the side walls of the strait and sinking in the center of the strait. These circulations form the horizontally convergent field in the cross-strait direction in the upper layers while the horizontal divergence takes place in the bottom layer. These vertical water-motions produce the three-dimensional distribution of velocity shear and phase lag of the tidal current around the strait, and the Lagrangian drifts of water particles become large. As a result, water exchange through the strait is greatly enhanced: The water exchange rate reaches 94.1% which is much larger than that obtained in the vertically integrated two-dimensional model. When the basins are stratified, the stable stratification suppresses the vertical motion so that a pair of vertical vortices are confined in the lower layers. This leads to a decrease in the exchange rate, down to 88.6%. Our numerical results show that the three-dimensional structure of tidal currents should be taken into account in tidal exchange through a narrow strait.     

Morphology, reproduction, ecology and isoenzyme electrophoresis of Capitella complex in Qingdao

-Morphology, reproduction, ecology and isoenzyme electrophoresis of a Capitella complex in Qingdao were examined from 1983 to 1984. We determined that the Capitella complex in Qingdao was composed of at least three sibling species: Capitella cf capitata, Capitella sp. I and Capitella sp. IT. Although these three species are very similar in morphology, they differ in: three kinds of isoenzyme electrophoresis, staining reactions, developmental pattern, larval morphology and dispersal, reproductive strategies, breeding season and population ecology. In addition, they show a series of minor quantitative variations in adult morphology, especially in number and morphology of setae. Such differences can be detected only at the population level. Some ecological data such as density, growth rate, mortality and sex distribution are also provided in this paper.     

龙口的海风及其影响

本文运用龙口气象站近５年自记风资料及实测探空资料，分析了龙口海风的生消规律、海风强度、空间结构及海风生成后对温、湿度的影响。同时为分析海风环流对大气污染物的影响。本文提出了一个简单地计算热内边界层的方法。     

内蒙古科右中旗两类基性岩的年代学、地球化学及其构造意义

兴蒙造山带东段晚古生代构造演化存在争议,基性岩浆作用是构造演化过程中的良好地质记录. 对贺根山缝合带东段内蒙古科右中旗构造混杂岩带内的杜尔基基性岩和甲哈达基性岩进行了系统的地质特征、岩相学、年代学和地球化学研究. 杜尔基基性岩岩性为枕状玄武岩和辉绿岩,辉绿岩锆石LA-ICP-MS U-Pb年龄为348.3±2.6 Ma,为低钾拉斑玄武系列,相对富集LILE,亏损Nb、Ta等高场强元素. 甲哈达基性岩岩性主要为玄武岩,锆石U-Pb年龄为317.6±3.0 Ma,为钙碱性系列,同样具有HFSE亏损和LILE富集的特点,与杜尔基基性岩相比更加富集LILE和LREE. 结合贺根山缝合带早石炭世蛇绿岩及洋内俯冲作用的研究成果,认为从杜尔基基性岩到甲哈达基性岩的演化,可能指示了古亚洲洋东段早-晚石炭世洋内俯冲的渐进过程,洋内弧从不成熟向逐渐成熟演化.      

天目山千亩田泥炭腐殖化度记录的中全新世气候变化

以14C测年为基础,构建了天目山千亩田地区泥炭沼泽剖面年龄-深度模式曲线,通过该区的腐殖化度与烧失量分析研究,发现两者呈现出很好的相关性,共同记录了该地区4 200 aBP以来的气候变化,可划分出3个主要阶段:早期(4 135±40~3 200 aBP)泥炭腐殖化度偏低,指示气候湿热;中期(3 200~630 aBP)泥炭腐殖化度偏高,指示气候温凉偏干;晚期(630 aBP以来),泥炭腐殖化度偏低,气候温凉湿润。     

基于光合有效辐射的西北太平洋柔鱼资源丰度和空间分布变动研究

光合有效辐射(PAR)对于海洋初级生产力具有显著的调控作用,会间接影响到头足类的资源丰度和渔场分布。本文根据2006−2015年上海海洋大学鱿钓科学技术组提供的柔鱼鱿钓数据和光合有效辐射卫星遥感数据,研究了光合有效辐射与西北太平洋柔鱼种群的关系,并评估了异常环境条件下光合有效辐射对柔鱼的影响。结果发现,柔鱼单位捕捞努力量渔获量(CPUE)、渔场纬度重心、PAR,及其空间分布均具有显著的月间变化。7−11月各月适宜PAR范围分别为36～39 E/(m²·d)、33～36 E/(m²·d)、24～27 E/(m²·d)、18 E/(m²·d)和12 E/(m²·d),最适宜PAR值分别为36 E/(m²·d)、33 E/(m²·d)、27 E/(m²·d)、18 E/(m²·d)和12 E/(m²·d)。此外,CPUE与PAR有显著正相关关系(p<0.05),且CPUE与各月适宜PAR范围占渔场比例也呈显著正相关(p<0.05);同时渔场纬度重心随各月最适PAR平均纬度变化而变化。在异常环境条件下,拉尼娜年份CPUE值比厄尔尼诺年份高,该年份PAR值异常增高可能是导致柔鱼资源丰度增加的因素之一。研究表明,光合有效辐射对于西北太平洋柔鱼资源丰度和空间分布具有显著影响。     

发光细菌法在环渤海污水样品遗传毒性检测中的应用

建立了以经过遗传改造的发光细菌Acinetobacter sp.Rec A为受试物种的环境污染物遗传毒性快速检测方法,该方法最快可在3h内得到毒性评价结果。采用该方法评价了环渤海排污口12份污水样品的遗传毒性。环渤海12个排污口的污水样品均表现出了不同水平的遗传毒性,并呈现出一定的分布特征。其中,高毒水质集中出现在山东半岛污水样品中,中毒水质集中出现在辽东半岛地区,而低毒水质主要集中于京津冀地区。综上,在环渤海的污水样品遗传毒性检测中,这种新型发光细菌法具有快速、灵敏、简便等优点,可为以后近海环境的水质生物毒性检测提供参考依据,具有应用于近海环境水质快速监测与评价的潜力。     

2，3，4－三氟硝基苯的合成研究

提出以２，６－二氯苯胺为原料，经重氮化、氟硼酸重氮盐热分解、硝化、氟化等步骤，制成２，３，４－三氟硝基苯。优点是工艺简单，收率较高．