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大黄鱼(Larimichthys crocea)是我国重要的海产经济鱼类。因多年未加选育的累代养殖,养殖大黄鱼生长缓慢,性早熟、免疫力低下,亟需对养殖大黄鱼进行遗传改良。多样性芯片技术(Diversity arrays technology,DAr T)具有高通量和低成本的显著特点,不需要明确物种的基因组DNA序列信息,因而广泛应用于动植物遗传图谱制作和遗传多样性分析。本研究旨在采用DAr T技术鉴定与"东海1号"大黄鱼体长相关分子标记。首先随机测得"东海1号"大黄鱼199个个体体长,均值为13.45cm,符合正态分布(P0.05),"极端大群体"和"极端小群体"之间差异显著(P0.01)。然后采用7组限制性内切酶组合(Pst I/Alu I、Pst I/Ban II、Pst I/Bsp1286I、Pst I/Bst NI、Pst I/Hae III、Pst I/Rsa I、Pst I/Taq I)分别进行酶切,获得基因组代表性DNA片段并用于文库构建。根据多态性克隆数和多态性率确定Pst I/Rsa I酶切组合为最优降低基因组复杂度方法。之后从Pst I/Rsa I基因组代表性DNA片段文库中扩增获得3360个片段,以此为多样性芯片探针进行芯片点制,杂交筛选获得18个大黄鱼体长相关DAr T候选标记。经过新群体样本再次验证,仍有8个DAr T标记与体长相关。本研究有助于选育生长性状优良的大黄鱼群体。  相似文献   
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Malik  Ishfaq Hussain 《Natural Hazards》2022,110(3):1911-1929

Flood relief and rescue form an important basis of disaster management, and the assessment of flood damage is a critical component of flood risk management. In its recent history, Kashmir Valley witnessed the floods in 2014, 2015, 2017, 2019, 2020, and 2021, but the worst flood in the living memory of the people was witnessed in the year 2014, which created widespread loss in economic and societal aspects. The present study discusses the spatial dimension of impact, relief, and rescue of the flood of 2014 in the Kashmir Valley. It analyses the distribution of relief and politics of relief and rescue and highlights the role of the communitarianism and the heroics of the community members in dealing with floods. The study provides the data of relief distribution under different government schemes and reveals that the relief was not distributed equally in various districts of the valley. The study relies on primary and secondary sources of data. Ethnographic approach was used for acquiring primary data because it provides the complex narratives of disasters and the political and social rupture experienced during the disasters. The data have been analysed with the help of Geographic Information System.

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Geotechnical and Geological Engineering - Peat is a problematic or weak soil derived from fossilized organic material. The characteristics of peat like low shear strength (3–16 kPa),...  相似文献   
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We have produced brightness and magnetic field maps of the surfaces of CV Cha and CR Cha: two actively accreting G- and K-type T Tauri stars in the Chamaeleon I star-forming cloud with ages of 3–5 Myr. Our magnetic field maps show evidence for strong, complex multipolar fields similar to those obtained for young rapidly rotating main-sequence stars. Brightness maps indicate the presence of dark polar caps and low-latitude spots – these brightness maps are very similar to those obtained for other pre-main-sequence and rapidly rotating main-sequence stars.
Only two other classical T Tauri stars have been studied using similar techniques so far: V2129 Oph and BP Tau. CV Cha and CR Cha show magnetic field patterns that are significantly more complex than those recovered for BP Tau, a fully convective T Tauri star.
We discuss possible reasons for this difference and suggest that the complexity of the stellar magnetic field is related to the convection zone; with more complex fields being found in T Tauri stars with radiative cores (V2129 Oph, CV Cha and CR Cha). However, it is clearly necessary to conduct magnetic field studies of T Tauri star systems, exploring a wide range of stellar parameters in order to establish how they affect magnetic field generation, and thus how these magnetic fields are likely to affect the evolution of T Tauri star systems as they approach the main sequence.  相似文献   
88.
Four short core samples were collected from the creek, estuarine regions of southeast (SE) coast of India affected by the 2004 Asian Tsunami. The study is aimed to signify the importance of ostracoda species in identifying major natural events (e.g. Tsunamis) in the coastal regions. The presence of many marine ostracoda species in the beach areas and the comparative studies with earlier reports from SE coast indicate that these species were brought by the high-energy tsunami waves. The depositional feature of ostracoda species in the beach and estuarine region also infers on the nature and force of tsunami waves in a particular region. The results clearly support that microfossils can be used to identify the major natural events close to coastal regions.  相似文献   
89.
Microwave sensors having all-weather capabilities provide an opportunity to monitor rice grown in monsoon season. An attempt has been made to identify rice crop using multitemporal ERS-1 SAR data in C-band (5.3 GHz). Data acquired on August 15 (D1), September 19 (D2), October 24 (D3) and November 28 (D4) 1993 were taken. Combinations of data acquired on different dates were used for identification of rice crop. Single-date IRS-1B LISS II data in visible and NIR bands acquired on October 23, 1993 was also used for comparison of estimated rice area. Analysis of the results has shown that a combination of SAR data acquired at the tillering (August), booting (September) and heading (October) stages of rice crop enabled identification and area estimation of rice crop grown under lowland conditions. Single-date SAR data acquired in the month of October was found to be better for identification of rice compared to other dates.  相似文献   
90.
Second-order moments of the breakdown coefficients q r,l of the squared velocity derivative in plane and circular jets indicate a value of the exponent which is larger than that obtained by other methods using the same data or that previously reported by other workers using the same method on atmospheric data. The difference may be due to a failure of the present data to satisfy the first condition of scale similarity, possibly because of the relatively small Reynolds numbers of the laboratory flows. The dependence of the variance of ln q r,l on the length scale l appears to be independent of Reynolds number.  相似文献   
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