麻痹性贝毒毒素研究Ⅰ.塔玛亚历山大藻的麻痹性贝毒毒素对运动神经末梢和全细胞钠离子通道的抑制作用研究

麻痹性贝毒(Paralytic Shellfish Poisoning，PSP)毒素由石房蛤毒素(saxitoxin，STX)及其衍生物组成，目前己发现20余种，在赤潮研究、分子生物学和神经生物学基础研究、医药、军事防化等方面都有应用潜力[其结构、类型和应用见本集刊王云峰等(2003)“麻痹性贝毒毒素的应用研究进展”一文]。由于PSP毒素的稀有来源和国际社会对STX交易的禁止，限制了国内PSP毒素应用及研究的全面深入展开，因此本文作者对PSP毒素进行了制备，并采用不同方法对制备的PSP毒素进行了研究。 PSP毒素能够选择性地可逆抑制可兴奋膜的电压依赖钠离子通道的开放，从而阻止神经冲动的发生和传导，使神经、肌肉丧失兴奋性(Frace et al.，1986；Penzotti et al.，1998)。本文利用神经束膜下记录和全细胞膜片钳技术，报道了从塔玛亚历山大藻(Alexandrium tamarense)中提取的PSP粗毒素对小鼠运动神经末梢膜电流和NG108-15细胞钠离子通道的作用研究结果，并与STX标准毒素的作用结果进行了比较。 NG108-15细胞是由小鼠神经母细胞瘤和大鼠胶质细胞瘤融合的杂交细胞，经分化剂分化后，显示诸+L1196如兴奋性、合成和释放乙酰胆碱、与培养肌细胞形成突触联系等多种神经细胞的基本特性(Hamprcht，1977)和Na+、K+、Ca2+等多种离子通道，已作为神经细胞模型被广泛应用于分析药物对离子通道作用的研究(Enomoto et al.，1992； Docherty et al.，1992；Shi et al.，1993；Hu et al.，1997a；Hu et al.，1997b)；发育出具有Na+内流支持的锋电位(Hamprecht,1977)，该电位是分析作用于膜钠离子通道药物的好材料。     

考虑非线性弥散影响的波浪变形数学模型

提出了逼近Kirby和Dalrymple的非线性弥散关系的显式非线性弥散关系的表达式,该显式表达式与他们的非线性弥散关系的精度几乎完全相同.采用显式非线性弥散关系,结合含弱非线性效应的缓坡方程,得到考虑非线性弥散影响的波浪变形数学模型,并对该数学模型进行了数值验证.结果表明,考虑非线性弥散影响的波浪变形数学模型更为精确.     

利用卫星测高技术监测厄尔尼诺和拉尼娜现象

利用美国宇航局和法国空间局联合公布的T／P数据（1993-2000年）和Jason-1数据（2002—2006年），由共线法计算了热带太平洋地区海平面高度的变化。根据T／P和Jason-1数据计算的海面月变化异常图，分别研究了和分析了1997-1998年的厄尔尼诺和拉尼娜现象、2002-2003年厄尔尼诺现象的变化发展过程。     

ELASTIC CLASSIFICATION OF MODIFIED WATER MASS IN SHALLOW SEA

In this paper, the principle and steps for differentiating water masses by fuzzy cluster method are introduced, and a scalar formula based on Euclidean distance and a method for determining objectively the number of water masses by F-test are proposed. Consequently, a method and specific steps for differentiating modified water masses in shallow sea according to fuzzy elastic classification are given. Computation of the membership degree in which each sample belongs to every water mass determines conveniently and quantitatively the cores, boundaries of water masses and mixed zones. An example for the Huanghai Sea and East China Sea is shown and compared with previous results.     

Embryonic and larval development of Babylonia formosae habei (Altena and Gittenberger, 1981) (Gastropoda:Buccinidae) on China''''s coast

The development of embryos and larvae of Babylonia formosae habei living along the southeast coast of China is observed under laboratory conditions. The egg masses are laid by females on hard substrate at night and each capsule contains 100-500 eggs. Each egg is 250-280 mm in diameter. The first two cleavages of the embryo are meridional and equal, and a polar lobe is produced. Larval kidney, which only consists of a single cell, appears during the gastrula stage on each side of the embryo. The right tentacle develops prior to the left one. At 25-27℃, an intracapsulate veliger stage is reached about 4.5 d after deposition. The larvae hatch on the fifth day as swimming veligers with a shell length of 360 -500 mm. The newly hatched larva can ingest suspended algal cells from the water column and remains in the pelagic stage for 8-10 d. The newly settled juveniles are 900-1 200 mm in shell length.     

基于模拟退火算法的饲料配方设计

本文在饲料配方设计中首次引入新颖、高效的组合优化算法--模拟退火算法，并获得理想的数值结果，说明了在传统的饲料配方设计中高技术的应用可以获得更高的效益.     

黄海海域陆相中生界油气远景

黄海海域油气勘探已40余载，尚未实现油气突破，其找油前景引起石油地质学家的极大关注。根据前人的认识和最新研究成果，结合邻区油气地化资料，探讨了黄海海域中生界地层生、储、盖特征，特别是白垩系，它分布广，厚度大，烃源岩达到较好生油岩标准，并且生、储、盖组合理想，中生界是该区下部油气勘探首选目标。     

水下滑翔器的运动建模与分析

介绍了水下滑翔器的工作机理，对其沉浮阶段的滑翔过程进行了动力学分析，推导了滑翔器在垂直剖面上的动力学方程。论文深入分析了水下滑翔器稳态时的运动规律，以水下滑翔器试验模型为例，推导了其稳态运动参数，通过线性化与适当的简化，得到模型在垂直剖面上的运动状态方程，讨论了系统的可控性与可观测性，为水下滑翔器系统的开发设计和控制提供了理论依据，具有重要的指导意义。     

Embryonic and larval development of Babylonia formosae habei(Altena and Gittenberger, 1981)(Gastropoda: Buccinidae) on China''s coast

The development of embryos and larvae of Babyloniaformosae habei living along the southeast coast of China is observed under laboratory conditions. The egg masses are laid by females on hard substrate at night and each capsule contains 100-500 eggs. Each egg is 250-280 mm in diameter. The fast two cleavages of the embryo are meridional and equal, and a polar lobe is produced. Larval kidney, which only consists of a single cell, appears during the gastrula stage on each side of the embryo. The right tentacle develops prior to the left one. At 25-27 ℃, an intracapsulate veliger stage is reached about 4.5 d after deposition. The larvae hatch on the fifth day as swimming veligers with a shell length of 360 -500 mm. The newly hatched larva can ingest suspended algal cells from the water column and remains in the pelagic stage for 8-10 d. The newly settled juveniles are 900-1 200 mm in shell length.     

珠江口盆地基底结构的综合地球物理研究

通过对珠江口盆地重磁资料的分析处理及反演计算，依据盆地的地质、地球物理特征，采取了针对性的综合地球物理研究思路与方法，对地震资料难以解决的珠江口盆地基底结构进行了研究，确定了盆地的重力和磁性基底深度，并对盆地内的断裂和基底岩性作了推断，提供了新的认识，为今后研究提供了依据。