Way forward to achieve sustainable and cost-effective biofuel production from microalgae: a review

Driven by policies aimed at enhancing energy security and mitigating greenhouse gas emissions, the production and use of biofuels have significantly increased in recent years. Microalgae owing to its multiple advantages which include high lipid content, sustainable biomass production, effective land and water utilization are the most potential biofuel feedstock that can provide drop-in fossil fuel replacements without stimulating competition for agricultural resources and are considered to be more environmentally benign than the first- and second-generation biofuel feedstocks. However, there are many existing technical and scientific impediments that are yet to be resolved. Keeping this in view, the present review provides a concise account of the microalgal species known to accumulate high levels of lipid and describes the main factors that should be taken into consideration while selecting suitable algal strains for mass cultivation. The underlining advantages and limitations of raceway pond and photobioreactor cultivation systems are also examined. The recent advances in genetic engineering of microalgae to improve biomass and lipid productivity are then highlighted, which include the ongoing debate over the biosafety issues pertinent to the use of genetically modified algae. Furthermore, a wide range of high-value products that can be co-produced from microalgae have been discussed. The review concludes with a comprehensive summary of the major techno-economic constraints to commercialization of algal-derived biofuels along with promising methods for overcoming these challenges in order to produce cost-competitive and environmentally sustainable biofuel.     

Influence of varying nitrogen levels on lipid accumulation in <Emphasis Type="Italic">Chlorella</Emphasis> sp.

Microalgal lipids can be enhanced through varying nitrogen (N) content, and limited supply of nitrogen source seems to be valuable approach for increased lipid accumulation in microalgae. In this study, Chlorella sp. IM-02 was observed under fluorescence microscope for increased number of lipid bodies under nitrogen scarcity. Fourier transform infrared spectroscopy was used to determine spectral changes due to varying lipid content under nitrogen-starved (N₀, without sodium nitrate), nitrogen-limited (N_0.1, N_0.25, N_0.5 and N_1.0 representing 0.1, 0.25, 0.5 and 1.0 g/L of sodium nitrate, respectively) and nitrogen-sufficient (N_1.5, i.e., 1.5 g/L sodium nitrate) setting. Chlorophyll content was also monitored under these conditions as growth indicator. Various biochemical components viz. total carbohydrates, total proteins and total lipids were also estimated under varying nitrogen levels spectrophotometrically. On fourth day itself, maximum lipid productivity was observed in case of N_0.5, which is having one-third of nitrogen concentration present in original growth media, BG-11. This concludes N_0.5 as suitable nitrogen provision for better production of lipids in Chlorella sp. IM-02 without much compromising the biomass production as both growth and lipid quantity are key parameters affecting the lipid productivity of any microalgal strain.