中国东南沿海潮灾与防潮对策研究

中国东南沿海是中风暴潮灾害的最严得岸段，本文针对闽，粤，海南三省台风暴潮灾害概况，防潮现状及其存在问题，提出防潮减灾的对策和见解，以祈提醒人们居安思危，防患未然。     

浙江近海大黄鱼 Pseudosciaena crocea( Richardson )的性成熟特性

鱼类性成熟的开始是鱼类生命周期中转入繁衍后代的一个重要阶段。同一世代的个体开始性成熟的年龄、大小,以及延续期限与速度,是种和种内种群对水域生活条件的适应属性之一。 研究鱼类性成熟过程的特点,不仅在解决鱼类种群数量的预测与控制问题中,对补充过程的了解具有重大的意义,而且也为制订合理的渔业经营管理措施提供必要的生物学依据。 本文主要在于探讨浙江近海大黄鱼种群的性成熟特点及其影响因子;其次,讨论该特点在这一种群数量上的意义;并对捕捞规格(法定许可捕捞的鱼体大小和年龄等生物学指标)问题提出初步意见。 在这项工作中,单才根、滕文法和蓝志满等同志曾协助搜集资料、耳石制片和计算工作。在资料的收集中,得到洞头公社和温州地方水产部同的大力支持;本文承张孝威教授热情指导,井承成庆泰副教授等提出宝贵意见,均此谨表谢忱。     

Diagnosis of iridovirus in large yellow croaker by PCR

A rapid and sensitive PCR-based method for the detection of the large yellow croaker iridovirus(LYCIV) is described,which involves the amplification of a 295 bp fragment of the LYCIV ATPase gene from DNA isolated from naturally infected fish spleen.Sequencing of LYCIV ATPase gene fragment showed it shared 100% nucleotide sequence homology with the corresponding region of the ATPase gene of red sea bream iridovirus(RSIV) and sea bass iridovirus(SBIV),suggesting that LYCIV was homologous with RSIV and SBIV at least in part of the gemone.The specificity and sensitivity of the PCR procedure were tested on the iridovirus-infected fishes,the expected fragment was detected from spleen DNA samples of infected fishes,whereas no fragments were amplified from healthy fish spleen DNA,white spot syndrome baculoviruses(WSBV) DNA and pseudorabies virus(PRV) DNA.Detection limit of this method was 10^-7 ng positive plasmid DNA containing target sequence,equal to about 100 virions.In the infected experiment,first positive detection(1/4) appeared at Day 3 post-infection,all fish(4/4) tested positive at Day 7,however obvious symptoms were observed at Day 8,so LYCIV infection could be detected prior to the appearance of obvious symptoms.These results indicate that this PCR method could be used for early,rapid and specific detection of LYCIV infection.     

Experimental investigation on a two-part underwater towed system

An experimental set-up is developed and proved to be effective for laboratory study of an underwater towed system. The experimental technique gives a practical method for monitoring the kinematic and dynamic performance of an underwater towed system in a ship towing tank. Both the theoretical and experimental results in the investigation indicate that the hydrodynamic response of a towed vehicle to the wave induced motion of a towing ship can be significantly reduced by applying a two-part tow method. A comparison of the numerical and experimental results in the investigation demonstrates that the numerical simulation results are close to the experimental data, overall agreement between experimental and theoretical results is satisfactory. The results qualitatively verify the mathematical model of a two-part underwater towed system proposed by Wu and Chwang [Wu, J., Chwang, A.T., 2000. A hydrodynamic model of a two-part underwater towed system. Ocean Engineering 27 (5), 455–472].     

盐藻和β-胡萝卜素研究述评

养殖盐藻生产β-胡萝卜素是近十几年新兴起的研究领域，因其应用前途广内，现已成为微藻生物学的研究前沿和热点，预计本世纪末和下世纪初将有更大发展。鉴于我国在这方面文章不系统、不全面，本文从盐藻生物学、养殖学、β-胡萝卜素异构体、β-胡萝卜素形成生理等角度全面概述了该领域的研究现状，并提出存在的问题与研究方向。     

Outer wave number spectrum and statistical distribution of apparent wave number vector

Based on the ray theory and Longuet-Higgins’s linear model of sea waves, the joint distribution of wave envelope and apparent wave number vector is established. From the joint distribution, we define a new concept, namely the outer wave number spectrum, to describe the outer characteristics of ocean waves. The analytical form of the outer wave number spectrum, the probability distributions of the apparent wave number vector and its components are then derived. The outer wave number spectrum is compared with the inner wave number spectrum for the average status of wind-wave development corresponding to a peakness factor P = 3. Discussions on the similarity and difference between the outer wave number spectrum and inner one are also presented in the paper.     

几种红藻和蓝藻的光合作用色素

分离纯化出几种海产红藻和一种蓝藻的光合作用色素,并测定了它们的化学性质和光谱学性质。这些藻类是3种红藻:多管藻(Polysiphonia urceolata)、橡叶藻(Phycodrys sp.)和条斑紫菜(Porphyra yezoensis);蓝藻:钝顶螺旋藻(Spirulina platensis)。用羟基磷灰石柱层析法从上述藻类中分离到几种不同的藻胆蛋白。经SDS-PAGE及光谱测定,发现条斑紫菜中的藻红蛋白不同于其它两种红藻。而橡叶藻中存在的两条藻红蛋白也有差异,条斑紫菜和钝顶螺旋藻中的两种别藻蓝蛋白之间也有区别。叶绿素分析表明,钝顶螺旋藻中叶绿素a的含量高于红藻中叶绿素a的含量。     

The effects of sediment from an industrial outfall area on a marine planktonic ecosystem

-Sediment from Xiamen industrial area was added to marine ecosystem enclosures to test the effects on the planktonic communities in Xiamen Bay, China, in April, 1985. Sediments were added at two concentrations, 11.2 and 112 ppm dry wt. respectively. The species of phytoplankton did not change as compared with the control, but the number of diatoms markedly decreased in the sediment treated enclosures. A suppression of photosynthesis was presumed to be due to light attenuation by the added sediment. Microflagellates, zooplankton and bacteria were resistant to sediment increase in water column.     

裙带菜原生质体的分离和培养

本文以裙带菜(Undaria pinnatifida(Harv.)Suringar)为材料,进行原生质体分离和培养研究。取得以下结果: 1、使用海螺酶(sea snail enzymes)和纤维素酶(cellulase,Onozuka R-10),酶解裙带菜细胞壁,在一定的条件下,能够大量地分离成活的裙带菜原生质体。 2、实验表明,氯化钠可作为分离裙带菜原生质体研究中的一种理想的渗透刑。 3、荧光增白剂染色镜检法可作为鉴定裙带菜原生质体的一种辅助方法,并适用于原生质体再生壁的观察。 4、光照(2000—2500Lux)能促使原生质体再生细胞壁,含有蔗糖(W/V,1％)的培养液有助于原生质体再生细胞壁。 5、分离的裙带菜原生质体,经培养,已能长成幼体。     

2000年夏季莱州湾生态环境要素的分布特征

论文依据 2 0 0 0年夏季 8月 2 9日至 9月 2日莱州湾的 1次综合性生态环境调查资料 ,给出了表、中、底 3层的平均温度、盐度、主要营养盐浓度及其比例和叶绿素 a浓度 ,分析了莱州湾的温盐结构、主要营养盐和叶绿素 a的分布特征。由于莱州湾的水深较浅 ,各要素的垂直分布都比较均匀。生态环境要素的水平分布表现为小清河口为高温、低盐、高营养盐和高叶绿素 a浓度区 ,小清河口东测的湾顶区域为高温、高盐、相对低的营养盐和叶绿素 a浓度区。此次观测到的盐度较 1997年以前有明显升高。莱州湾各层平均的 N/ P和 Si/ N分别为 16.73和 1.67,都比 1998～ 1999年渤海中部的值大 ,但 N/ P比 1992 ,1995和 1996年莱州湾的 N/ P明显偏低。叶绿素 a浓度与硅酸盐浓度之间有较好的相关关系 (α=0 .0 1) ,表、中、底 3层叶绿素 a浓度同硅酸盐浓度的线性相关系数分别为 :0 .5 4 ,0 .68和 0 .67