模糊数学法在海洋水质评价中的应用

根据福建东碇临时海洋倾倒区2007-2009年间实施的5次海水水质监测资料,应用模糊合成-加权平均复合模型对该区及周边海域水质进行综合评价,分析倾倒活动对水质的影响情况,并与单参数特征值法评价结果相比较.结果表明:应用模糊合成的原理,根据最大隶属度原则评价水质,体现了分级界限的模糊性,其评价结果较单参数特征值法更接近客...     

深水压力舱密封技术研究

管道屈曲技术试验过程当中,发现深水压力舱的出厂自带密封工艺耗时过长、密封效果不好、安全性不佳、返工现象普遍.针对这一现象,采用全新的密封工艺流程与方法:在前期处理中对螺柱编号与清理以防止螺母安装过程中的卡死现象;采用自创的精度控制方法防止漏水以及返工;根据不同的工作压力对密封技术进行梯度划分,选取不同的密封方案.经过实...     

浙闽沿岸流消退时期的浮游植物群落结构特征

浙闽沿岸流对台湾海峡的水文环境意义重大.依据国家海洋局"908"补充调查专项,通过对覆盖浙闽沿岸流海洋锋面过程的海洋生物调查研究,于2008年4月实施针对浙闽沿岸流消退时期(春季)台湾海峡锋面海域的浮游植物取样分析.结果共记录3门41属86种,其中硅藻35属76种.甲藻5属9种,蓝藻1属1种.种类组成以暖水性种类和广布...     

真鲷（Pagrus major）vasa基因5′端启动子克隆及表达载体构建

采用染色体步移（Genome walking）的方法克隆了真鲷vasa基因5′侧翼序列,采用生物信息学方法分析潜在的顺式作用元件,并与斑马鱼核心启动子进行比对,在此基础上构建了绿色荧光蛋白表达载体。序列分析结果显示：克隆得到的真鲷vasa基因5′侧翼序列序列长度为2762bp,其中包括TATA-box、CAAT-box...     

乳糖诱导重组别藻蓝蛋白基因在大肠杆菌中的表达

以重组别藻蓝蛋白表达工程菌JM109（DE3）／pET28-APC作为研究对象，对于JM109（DE3）菌株在乳糖诱导下表达重组蛋白的规律进行了研究。比较分析了不同生长阶段进行诱导，最佳乳糖浓度、诱导持续时间和诱导温度等参数对重组蛋白表达的影响。实验结果表明，采用JM109（DE3）为宿主菌，经过条件优化，乳糖诱导重组蛋白的表达量可以达到IPTG诱导的水平；较低的诱导培养温度能有效提高可溶性重组蛋白的表达。最后在摇瓶发酵结果的基础上，实现了乳糖诱导工程菌的5L全自动发酵罐培养。研究结果为在大肠杆菌大规模发酵中，运用廉价、无毒的乳糖作为诱导剂提供了一定的借鉴。     

Variational iteration solving method for El Nio phenomenon atmospheric physics of nonlinear model

1 IntroductionThe El Ni’o atmospheric physics oscillation is anabnormal phenomenon involved in the tropical Pacificocean- atmosphere interactions. Studies on the El Ni(ophenomenon are very attractive (Lin et al., 2000; Linet al., 2001, 2002; Wang, 2001; Feng et al., 2001;Feng et al., 2002; Liu et al., 2002; Wei and Chen,2003; Xie et al., 2002; Zhu et al., 2002; Pu et al.,2003; Gu et al., 2004; Yu and Liu, 2004). Lin and Mo(2004), Mo and Lin (2004) and Mo et al. (2004) alsostudied a s…     

西沙湾工程波浪推算及波浪场数值模拟

本研究根据崇武水文站1976—2007年共31 a的波浪观测资料,使用皮尔逊Ⅲ型频率曲线进行拟合分析,推算出崇武在不同重现期条件下的特征波浪要素,再将其作为外海波浪要素输入,通过基于椭圆型缓坡方程的CGWAVE近岸波浪数值模型,模拟西沙湾的重现期波浪场,得到其设计波浪要素,得到较为合理的计算结果。西沙湾的海底地形强烈地影响近岸波浪,导致了波高和波向的不均匀分布特征,岛屿和礁石都是天然屏障,东侧水域受龟屿及闽台码头的掩护,波浪反射、绕射明显,对避风坞起到了有效的保护作用。此外,对于不同地质的边界,反射系数不同使得近岸波高分布有较大差异。     

中国卤虫早期胚胎发育相关基因的研究--mRNA差异显示技术的三种不同染色方法比较

利用mRNA差异显示技术研究中国孤雌生殖卤虫胚胎发育的相关基因，并对中国孤雌生殖卤虫差异显示中SYBR Green Ⅰ、硝酸银和溴化乙锭三种染色方法进行了比较。以三种单碱基锚定引物对发育0h，4h，8h，12h，16h和10天的中国孤雌生殖卤虫的RNA进行逆转录和26种随机引物进行PCR反应，经非变性的6％聚丙稀酰胺凝胶电泳后，分别用SYBR Green Ⅰ、硝酸银和溴化乙锭染色，最后进行差异条带的回收和再扩增。结果表明：(1)6种样本的DD-PCR产物显示长200-3．0k不等的扩增片段，条带清晰，并呈现出有无和强弱的变化(2)SYBR Green Ⅰ和硝酸银染色的灵敏度比溴化乙锭染色的灵敏度高(3)经SYBR Green Ⅰ和溴化乙锭染色的凝胶的回收和再扩增效率比硝酸银染色的高(4)水煮法和NAP-25过柱法对凝胶片段的回收效果几乎相当。利用mRNA差异显示技术研究中国孤雌生殖卤虫胚胎发育相关基因时，得到了较好的扩增片段；三种染色方法中SYBR Green Ⅰ染色灵敏度较高，并且SYBR Green Ⅰ染色有利于凝胶片段的回收和再扩增。     

Parabolic Wave Propagation Modelling in Orthogonal Coordinate Systems

This paper presents a numerical model study of the propagation of water waves using the parabolic approximation of the mild-slope equation in the orthogonal coordinate system. Two types of coordinate systems are studied: (a) a general form of orthogonal coordinate system and (b) the conformal system, a special form of orthogonal coordinate system. Two typical examples, namely, expanded breakwaters and a circular channel, are studied to validate the model. First, the examples are studied by use of the general orthogonal coordinates. Then the same examples are computed by use of the confonnal system. The computational results show that the confonnal coordinate system generally gives better predictions than the general orthogonal system. A numerical technique for generating the conformal grid is combined with the numerical model to improve the practicability of the model. The comparison between the result from the numerical grid system and that from the analytical grid system shows that reliable computational results can be obtained by use of the numerical confonnal grid system.     

Influence of water logging time on the growth of candel seedlings

Influence ofwaterlogging time on the growth ofKandeliacandel(L.) Druce seedlings grown for 70 d in the artificial-tidal tanks' simulated semidiumal tide under greenhouse is studied. Sand and soil act as the substrate and artificial sea-water with salinity of 15 is used in cultivation. Shorter waterlogging time (inundated for about 2 ～ 4 h) promotes thegrowth of K. candel seedlings, while longer time(inundated more than 8 h) or no waterlogging(0 h) inhibits theirgrowth. The number and length of aerating roots increase with the increase ofwaterlogging time. Under existing condi-tions, the optimalwaterlogging time for the growth of K. canoel seedlings is about 2 ～ 4 h in every tide cycle. Com-pared with other treatments, the 2 h sanded treatments obtain the highest biomass of seedlings, have the lowest massloss ofhypocotyl and broaden the photosynthetic area by increasing the area per leaf after 70-d cultivation. And the soiltreatments have the similar tendeney. However, waterlogging for 8 h in every tide cycle is critieal for normal develop-ment of seedlings. K. candel seedlings are highly tolerant to waterlogging and a proper waterlogging is beneficial to thegrowth ofK. candel seedlings.