斑鳢(Channa maculata)微囊藻毒素去毒相关基因sGST的克隆与序列分析

采用RT-PCR技术和RACE技术成功克隆淡水鱼类斑鳢sGST基因cDNA全序列,推测得到氨基酸序列,初步分析其结构功能域及系统进化关系。结果表明,斑鳢sGST基因cDNA序列全长为898bp,编码225个氨基酸。斑鳢sGST与真鲷、金头鲷、鲽、黑头鲦、川鲽、大口黑鲈等最新定名为ρ型的sGST氨基酸同源性较高,ρ型sGST为水生生物所特有并共同占据进化树上独立的分枝;与大鼠、小鼠、人等哺乳动物sGST现有所有类型同源性均很低,并且在进化树上距离也较远,表明本研究成功克隆的sGST基因应属于ρ型,可能在鱼类等水生生物对水栖环境的适应上有重要作用。     

白斑综合症病毒(WSSV)的宿主调查

用地高辛 (DIG)标记的WSSVDNA探针斑点杂交与原位杂交技术 ,在中国对虾、斑节对虾、南美白对虾、刀额新对虾、脊尾白虾、天津厚蟹、日本大眼蟹体内检测到了WSSV ,它们是WSSV的天然宿主 ;在经人工感染的哈氏美人虾、短脊鼓虾、克氏原螯虾、肉球近方蟹、滕壶体内检测到了WSSV ;在球形侧腕水母、病虾池的桡足类等浮游生物、卤虫无节幼体以及人工浸泡感染卤虫成体体内没有检测到WSSV。经原位杂交检测 ,虾类的甲壳下上皮、胃上皮、附肢、造血组织、鳃等组织器官均可被WSSV侵染 ,其中甲壳下上皮和鳃对WSSV敏感 ;蟹类的甲壳下上皮和鳃对WSSV敏感 ;在中国对虾、南美白对虾、脊尾白虾、注射感染的克氏原螯虾的精巢中 ,精荚的结缔组织细胞和血细胞呈阳性 ,在中国对虾、脊尾白虾以及注射感染的短脊鼓虾的卵巢中 ,结缔组织细胞和滤泡细胞被WSSV感染。     

近58a来影响和登陆浙江的热带气旋统计特征分析

本文利用1949-2006年热带气旋资料,对58a来影响和登陆浙江热带气旋的登陆位置、登陆时间的年际、月际和日变化、登陆后的移速及路径变化与降水中心分布的关系、登陆后维持和衰减情况等进行了统计分析.统计结果表明：影响和登陆浙江的热带气旋有较明显的年际、月际和日变化特征;登陆浙江的热带气旋陆上维持时间与登陆时的强度正相关,登陆后12h内热带气旋衰减较快;登陆后移向偏北分量大的或移速加快的热带气旋,主要降水区出现在路径右侧的可能性大.     

Effect of the Twin Jetties as A Regulating Structure at the Tao-er Estuary

The phase one project of the twin jetties at the Tao-er Estuary yields no effect on the flow, and there is a large area of deposition. A numerical model is employed to simulate the flow field, and the scouring and silting in the channel be-tween the two jetties are analyzed. The results show that the effective tidal flux between the two jetties is reduced due to the notch located at the heel of the east jetty, and the effect of contraction-flush is not obvious. As for the regulation of this kind of estuary under the tide action, the capability of tidal flux should be fully used.     

基于GIS空间统计分析的广电收视率评估研究——以北京市广电收视覆盖分析系统为例

本文将GIS空间信息统计分析手段应用于广播电视收视率的评估,以层次分析法为基础进行改进,采用抽样调查的方法确定权重,提出较为合理的基于空间信息统计方法的广电人口覆盖分布模型并加以软件实现。以北京市为研究区域,经导入多个版本人口数据实验,发现这种评估方式较好地修正了传统的仅仅按照面积评估覆盖率的评估模型,对于提高广电行业的信息化管理水平具有一定意义。     

基于MSA特征的遥感图像多目标关联算法

遥感图像中多目标关联存在以下两个问题:一是低时间分辨率观测使得目标状态信息无法准确估计,基于Kalman滤波的多目标关联算法不再适用;二是基于图像特征的目标关联算法又无法处理大场景观测中多个目标关联引起的模糊性.针对上述问题,提出一种基于多尺度自卷积特征匹配和关联代价矩阵最优化的多目标关联算法.实验表明该算法对遥感图像中多目标关联问题具有一定的适用性.     

TOPCON GTS901A机载程序设计

介绍了基于Windows CE嵌入式操作系统,开发了TOPCON智能型全站仪GTS901A机载程序的方法,实现了学习测量、自动测量、数据存储及处理等功能。     

Simulation experiment on hydrocarbon generating potential of various source rocks on the northern margin of the Qaidam Basin,Qinghai, China

The northern margin of the Qaidam Basin is one of the main oil-gas exploration areas in western China, where source rocks are composed mostly of Middle and Lower Jurassic dark mud shale, carbargillite and coal. A large number of subsurface and outcrop samples differing in lithology with different types of organic matter were selected for resource evaluation, research and calculation. And among them, 13 samples were used for simulation experiment on hydrocarbon generating potential of various source rocks. At first, two kinds of heating modes were compared through simulation experiment, including single temperature-step heating and continual heating. Perhaps, the process of hydrocarbon generation and expulsion occurred naturally between a close system and an open system. In addition, the first heating mode was convenient, and all its reaction products were involved in the whole thermal evolution, and the final simulation experimental results were basically in consistency with the natural evolution trend Therefore, the first heating mode was adopted and the hydrocarbon yield of every sample was worked out. According to the type and lithology of organic matter and the hydrocarbon yield of samples for simulation experiment, hydrocarbon generation and expulsion mode with three kinds of lithology and five types of source rock has been established to provide the basis for hydrocarbon generation evaluation, research and resource calculation.     

Formation Dynamics and Quantitative Prediction of Hydrocarbons of the Superpressure System in the Dongying Sag

Based on the theory of formation dynamics of oil/gas pools, the Dongying sag can be divided into three dynamic systems regarding the accumulation of oil and gas： the superpressure closed system, the semi-closed system and the normal pressure open system. Based on the analysis of genesis of superpressure in the superpressure closed system and the rule of hydrocarbon expulsion, it is found that hydrocarbon generation is related to superpressure, which is the main driving factor of hydrocarbon migration. Micro fractures formed by superpressure are the main channels for hydrocarbon migration. There are three dynamic patterns for hydrocarbon expulsion： free water drainage, hydrocarbon accumulation and drainage through micro fissures. In the superpressure closed system, the oil-driving-water process and oil/gas accumulation were completed in lithologic traps by way of such two dynamic patterns as episodic evolution of superpressure systems and episodic pressure release of faults. The oil-bearing capacity of lithologic traps is intimately related to reservoir-forming dynamic force. Quantitative evaluation of dynamic conditions for pool formation can effectively predict the oil-bearing capability of traps.     

地震瞬时属性分析技术在岩溶勘查中的应用

介绍地震瞬时属性分析技术的原理及处理方法,分别对岩溶在地震剖面、瞬时振幅剖面、瞬时频率剖面、瞬时相位剖面上的表现特征作了说明.并结合实际资料进行多参数解释,进而确定地下岩溶的发育情况、分布范围和规模,同时对异常进行钻井验证,结果表明,地震瞬时属性分析技术比常规地震处理方法能更好地排除干扰,确定岩溶异常.