Effects of heavy oil in the developing spotted halibut, Verasper variegatus

It is well known that heavy oil (HO) on the sea surface causes serious problems in the aquatic environment. In particular, some species of teleosts which develop on the sea surface are thought to be affected by the HO which flows out from tankers or coastal industry. However, the toxicological effects of HO are not fully understood. We performed exposure experiments using the Pleuronectiformean fish, spotted halibut (Verasper variegatus), which is an important fishery resource in Japan. In course of the development, HO-exposed embryos showed remarkable delay in developmental processes including somite formation. We further observed abnormal development of the head morphology. Notably, treated embryos had relatively small eyes and craniofacial structures. These findings strongly suggest that HO seriously affects the cell proliferation and differentiation of the embryo. In addition, HO-exposed embryos showed abnormal neuronal development. We also performed the exposure in the larval stage. Treatment of post-hatching larvae with HO resulted in significantly greater mortality compared with controls. Through these observations, we finally conclude that HO is strongly toxic to halibut in their early life stages.     

Toxicogenomic analysis of immune system-related genes in Japanese flounder (Paralichthys olivaceus) exposed to heavy oil

Heavy oil contamination is one of the most important environmental issues. Toxicities of polycyclic aromatic hydrocarbons (PAHs), including immune toxicities, are well characterized, however, the immune toxic effects of heavy oil, as a complex mixture of PAHs, have not been investigated. In the present study, we selected Japanese flounder (Paralichthys olivaceus) as a model organism, and observed alteration of immune function by the exposure to heavy oil. To analyze the expression profiles of immune system-related genes, we selected 309 cDNAs from our flounder EST library, and spotted them on a glass slide. Using this cDNA array, alteration of gene expression profiles was analyzed in the kidneys of flounders exposed to heavy oil. Six Japanese flounders (mean body weight: 197 g) were acclimated to laboratory conditions at 19-20 degrees C. Three fish were exposed to heavy oil C (bunker C) at a concentration of 3.8 g/L for 3 days, and the others were kept in seawater without heavy oil and used as the control. After the exposure period, the fish were transferred into control seawater and maintained for 4 days, and then they were dissected and their kidneys were removed. Total RNA was extracted from the kidney samples to use in gene expression analyses. The microarray detected alteration of immune system-related genes in the kidneys of heavy oil-exposed flounders, including down-regulation of immunoglobulin light chain, CD45, major histocompatibility complex class II antigens and macrophage colony-stimulating factor precursor, and up-regulation of interleukin-8 and lysozyme. These results suggest that pathogen resistance may be weakened in heavy oil-exposed fish, causing a subsequent bacterial infection, and then proinflammatory genes may be induced as a defensive response against the infection. Additionally, we found candidate genes for use as biomarkers of heavy oil exposure, such as N-myc downstream regulated gene 1 and heat shock cognate 71 kDa proteins.     

Effects of heavy oil in the developing spotted halibut, Verasper variegatus

It is well known that heavy oil (HO) on the sea surface causes serious problems in the aquatic environment. In particular, some species of teleosts which develop on the sea surface are thought to be affected by the HO which flows out from tankers or coastal industry. However, the toxicological effects of HO are not fully understood. We performed exposure experiments using the Pleuronectiformean fish, spotted halibut (Verasper variegatus), which is an important fishery resource in Japan. In course of the development, HO-exposed embryos showed remarkable delay in developmental processes including somite formation. We further observed abnormal development of the head morphology. Notably, treated embryos had relatively small eyes and craniofacial structures. These findings strongly suggest that HO seriously affects the cell proliferation and differentiation of the embryo. In addition, HO-exposed embryos showed abnormal neuronal development. We also performed the exposure in the larval stage. Treatment of post-hatching larvae with HO resulted in significantly greater mortality compared with controls. Through these observations, we finally conclude that HO is strongly toxic to halibut in their early life stages.     

Coronal general magnetic field evolution as a new parameter of the solar cycle

The magnetic field lines of the corona associated with the solar-cycle surface general magnetic field are calculated by a potential-field approximation to study the solar-cycle evolution of the geometry of the coronal field. The surface field evolution used here is the radial field evolution, predicted by a model of the solar cycle driven by the dynamo action of the global convection, and justified observationally using Mount Wilson magnetic synoptic chart data. The evolution of the calculated coronal general field is now good for comparison with observations and shows the following. (i) The field of the polar and high-altitude corona has dipolar structure in almost all phases of the solar cycle except in a short time interval around maximum phase despite the quadrupolar structure of the general magnetic field at the surface; quadrupolar field forms loop-like structure in the lower corona. The almost-dipolar structure of the polar and high-latitude corona and the loop formation of the equatorial lower corona explain the appearance of the undisturbed minimum corona observed at eclipses. (ii) The polar field lines are directed almost radially at the minimum phase, which should be responsible for polar plumes. The field lines slowly open up to participate in the loop-like structure of the equatorial lower corona, and rapidly change their structure and polarity at the maximum phase, to resume the almost radial configuration slowly, (iii) During the rapidly changing maximum phase, the field lines do not penetrate deep into the interplanetary space resulting in the absence of polar plumes and the appearance of the circular corona- the maximum corona. In this phase, the coronal field should not be approximated by a dipole field. The surface field evolution which can explain such behaviors of the corona is characteristic of the solar-cycle process dominated by the latitudinal gradient of the differential rotation. If the radial gradient dominated in the subsurface process, the coronal evolution would look quite different and would show latitudinal propagation of enhancement of activity. Although nonaxisymmetric features should be superposed on the axisymmetric general field to express the real corona, the general field can be a basic coronal field in studying long-term interaction between the convection zone and the interstellar space especially in studying the magnetic braking of the solar rotation.     

The secular modulation of solar rotation from 1943 to 1992 and its time-delayed correlation with the 55-year grand cycle of the 11-year solar cycle

We devised a new method, which we call the running-segment method, to achieve high-resolution time series of indices of solar rotation for determining the latitude dependence of the differential rotation by a least-squares fitting of the daily translation of positions of sunspot groups during a fixed time segment of 11 years. The segment is moved by an amount of one year to determine the differential profile of the next point of time. Time of the determined rotation data is defined by an arithmetic mean of the beginning and ending years of the segment. The rotation underwent an acceleration from 1948 to 1974 and a deceleration from 1974 to 1987. We found that the time profile of the indexM, the angular momentum surface layer density defined by integration of the angular momentum volume density over the whole surface, follows almost exactly the time profile of the 11-year running mean of the yearly mean of the sunspot relative number with a delay time of about 20 years. The acceleration (deceleration) phase corresponds to the ascending (descending) phase of amplitude of the 11-year solar cycle of cycle 16 (19) to cycle 19 (20) with a delay time of about 20 years. The cycles 15–20 correspond to the 55-year grand cycle V of the 11-year cycle. The delay time of about 20 years agrees well with the delay time predicted by a nonlinear dynamo theory of the solar cycle for driving the 55-year modulation of the 11-year solar cycle. The agreement suggests that the Lorentz force of the magnetic field of the solar cycle during grand cycle V drives the solar rotation modulation from 1948 to 1987 and that the force needed about 20 years to modify the rotation during 1943–1992.     

THE GALATIA,KANSAS, CHONDRITE

The Galatia meteorite was found in August, 1971, approximately 7 km ENE of Galatia, Barton County, Kansas (98° 53′W., 38° 39.5′N). The single stone weighed 23.9 kg and is partially weathered. Olivine (Fa_24.9) and pyroxene (Fs_20.9) compositions indicate L-group classification, and textural observations indicate that the stone is of petrologic type 6. Galatia is similar in many respects to the Otis L6 chondrite (found 20 km to the west), but it does not have the brecciated structure of Otis and, thus, it is not part of the same fall.     

TheKyucho in Uwajima Bay

The phenomenon of rapid increase in water temperature accompanied by a sudden, swift current (Kyucho) in Uwajima Bay is described on the basis of results of long term observations from July 1985 to September 1986. This phenomenon occurs somewhat periodically with an interval of about 15 days in summer, although it does not occur in winter. The increase in water temperature occasionally reaches about 5°C. This phenomenon results from the intrusion of a warm water mass into the bay. NOAA-9 satellite images show that the warm water mass originates from the Pacific Ocean south of the Bungo Channel.     

Dissociation of CAS phase in the uppermost lower mantle

The high-pressure stability limit of calcium aluminosilicate (CAS) phase has been examined in its end-member CaAl₄Si₂O₁₁ composition at 18–39 GPa and 1,670–2,300 K in a laser-heated diamond-anvil cell (LHDAC). The in-situ synchrotron X-ray diffraction measurements revealed that the CAS phase decomposes into three-phase assemblage of cubic Al-bearing CaSiO₃ perovskite, Al₂O₃ corundum, and SiO₂ stishovite above 30 GPa and 2,000 K with a positive pressure–temperature slope. Present results have important implications for the subsolidus mineral assemblage of subducted sediment and the melting phase relation of basalt in the lower mantle.     

Application of WetSpass model to estimate groundwater recharge variability in the Nile Delta aquifer

Long-term groundwater recharge from rainfall in the Nile Delta is needed as an input for integrated groundwater modelling in the Nile Delta aquifer for more accurate simulation. The main objective is to estimate the spatial and temporal variation of groundwater recharge from rainfall in the Nile Delta aquifer. Water and Energy Transfer between Soil, Plants and Atmosphere under quasi-Steady State (WetSpass) model parameters were identified for the Nile Delta based on the available meteorological data for the area collected in 1991 and 2000. The collected data were rainfall, temperature, wind speed and evapotranspiration. Geomorphological characteristics, such as soil type, topography, groundwater depth and slope, were also collected as input data for the WetSpass model. ENVI software was used to come up with land use classification based on available land cover images of the Nile Delta for 1972, 1984, 1990, 2000 and 2009. The WetSpass model was calibrated by comparing the simulated groundwater recharge with the calculated one by using the water balance equation model. The results indicated close agreement in groundwater recharge between the two model outputs with R ² of 0.99 and 0.94, while the root-mean-square errors (RMSEs) were 4.86 and 9.39 mm for 1991 and 2000, respectively. The WetSpass model was then applied in respect of 1970, 1980, 1990 and 2010 for the purpose of validation. The overall RMSE and R ² for the 6 years were 8.83 mm and 0.88, respectively. The results of the WetSpass calibrated model provide information to support integrated groundwater modelling. The results reveal that WetSpass works well in simulating the components of the hydrological balance in the Nile Delta.