Effects of nanoparticles in Mytilus edulis gills and hepatopancreas - a new threat to marine life?

Every day new extraordinary properties of nanoparticles (a billionth of a meter) are discovered and worldwide millions are invested into nanotechnology and nanomaterials. Risks to marine organisms are still not fully understood and biomarkers to detect health effects are not implemented, yet. We used the filter feeding blue mussel as a model to analyse uptake and effects of nanoparticles from glass wool, a new absorbent material suggested for use in floating oil spill barriers. In both, gills and hepatopancreas we analysed uptake of nanomaterials by transmission electronmicroscopy (TEM) in unstained ultrathin sections over a period of up to 16 days. Lysosomal stability and lipofuscin content as general indicators of cellular pathology and oxidative stress were also measured. As portals of uptake, diffusion and endocytosis were identified resulting in nanoparticle accumulation in endocytotic vesicles, lysosomes, mitochondria and nuclei. Dramatic decrease of lysosomal membrane stability occurred after 12h of exposure. Lysosomal damage was followed by excessive lipofuscin accumulation indicative of severe oxidative stress. Increased phagocytosis by granulocytes, autophagy and finally apoptosis of epithelial cells of gills and primary and secondary digestive tubules epithelial cells indicated progressive cell death. These pathological responses are regarded as general indices of toxic cell injury and oxidative stress. By the combinational use of biomakers with the ultrastructural localisation of nanoparticle deposition, final evidence of cause-effect relationships is delivered.     

The Namuskluft and Dreigratberg sections in southern Namibia (Kalahari Craton,Gariep Belt): a geological history of Neoproterozoic rifting and recycling of cratonic crust during the dispersal of Rodinia until the amalgamation of Gondwana

This paper presents combined U/Pb, Th/U and Hf isotope analyses on detrital and magmatic zircon grains together with whole-rock geochemical analyses of two basement and eight sedimentary rock samples from the Namuskluft and the Dreigratberg in southern Namibia (Gariep Belt). The sedimentary sections evolved during the Cryogenian on the SW part of the Kalahari Craton and where therefore deposited in an active rift setting during the break-up of Rodinia. Due to insufficient palaeomagnetic data, the position of the Kalahari Craton within Rodinia is still under discussion. There are possibilities to locate Kalahari along the western side of Australia/Mawsonland (Pisarevski et al. in Proterozoic East Gondwana: supercontinent assembly and break-up, Geological Society, London, 2003; Evans in Ancient Orogens and modern analogues. Geological Society, London, 2009; and others) or together with the Congo-Sao Francisco and Rio de la Plata Cratons (Li et al. in Prec Res 45: 203–2014, 2008; Frimmel et al. in Int J Earth Sci (Geol Rundsch) 100: 323–354, 2011; and others). It is sill unclear which craton rifted away from the Kalahari Craton during the Cryogenian. Although Middle to Upper Cryogenian magmatic activity is known for the SE Kalahari Craton (our working area) (Richtersveld Suite, Rosh Pinah Fm), all the presented samples show no U/Pb zircon ages younger than ca. 1.0 Ga and non-older than 2.06 Ga. The obtained U/Pb ages fit very well to the exposed basement of the Kalahari Craton (1.0–1.4 Ga Namaqua Province, 1.7–2.0 Ga Vioolsdrif Granite Suite and Orange River Group) and allow no correlation with a foreign craton such as the Rio de la Plata or Australia/Mawsonland. Lu–Hf isotopic signatures of detrital zircon point to the recycling of mainly Palaeoproterozoic and to a smaller amount of Archean crust in the source areas. εHf(t) signatures range between ?24 and +14.8, which relate to T_DM model ages between 1.05 and 3.1 Ga. Only few detrital zircon grains derived from magmas generated from Mesoproterozoic crustal material show more juvenile εHf(t) signatures of +14, +8 to +4 with T_DM model ages of 1.05–1.6 Ga. During Neoproterozoic deposition, only old cratonic crust with an inherited continental arc signature was available in the source area clearly demonstrated by Hf isotope composition of detrital zircon and geochemical bulk analysis of sedimentary rocks. The granodiorites of the Palaeoproterozoic basement underlying Namuskluft section are ca. 1.9 Ga old and show εHf(t) signatures of ?3 to ?5.5 with T_DM model ages of 2.4–2.7 Ga. These basement rocks demonstrate the extreme uplift and deep erosion of the underlying Kalahari Craton at its western margin before general subsidence during Cryogenian and Ediacaran time. The sedimentary sequence of the two examined sections (Namuskluft and Dreigratberg) proposes the presence of a basin and an increasing subsidence at the SW part of the Kalahari Craton during the Cryogenian. Therefore, we propose the initial formation of an intra-cratonic sag basin during the Lower Cryogenian that evolved later to a rift basin at the cratonic margin due to increasing crustal tension and rifting together with the opening of the Adamastor Ocean. As the zircons of the sedimentary rocks filling this basin show neither rift-related U/Pb ages nor an exotic craton as a possible source area, the only plausible sedimentary transport direction providing the found U/Pb ages would be from the E or the SE, directly from the heart of the Kalahari Craton. Due to subsidence and ongoing sedimentation from E/SE directions, the rift-related magmatic rocks were simply covered by the input of old intra-cratonic material that explains the absence of Neoproterozoic zircon grains in our samples. The geochemical analyses show the erosion of a continental arc and related sedimentary rocks with an overall felsic provenance. The source area was a deeply eroded and incised magmatic arc that evolved on continental crust, without any evidence for a passive margin. All of this can be explained by the erosion of rocks related to the Namaqua Belt, which represents one of the two major peaks of zircon U–Pb ages in all analysed samples. Therefore, the Namaqua Belt was well exposed during the Cryogenian, available to erosion and apart from the also well-exposed Palaeoproterozoic basement of the Kalahari Craton one potential source area for the sedimentary rocks in the investigated areas.     

On Probing the Inertial Subrange

For an extensive turbulent wind speed data set collected on the open ocean, the optimum sampling time is determined to calculate inertial-range spectra. Such spectra are widely used to estimate surface fluxes. The optimum sampling time corresponds to the turbulent memory time, the minimum period after which a given time series can be interpreted as a new independent measurement. In our case, this interval is found to be only about six seconds.Separate spectral levels are calculated from the three measured vector components of the wind. Their ratios are compatible with the assumption of an isotropic inertial subrange, but scatter considerably. Alignment factors are developed based on Kolmogorov's theory, and the aligned spectral levels coincide within a few percent. It is proposed that alignment might be a more reliable test than the ratio of the spectral levels to determine whether observations have taken place in a universal inertial subrange. It is recommended that all three wind components are used to estimate the dissipation rate.     

LakeCC: a tool for efficiently identifying lake basins with application to palaeogeographic reconstructions of North America

Along the margins of continental ice sheets, lakes formed in isostatically depressed basins during glacial retreat. Their shorelines and extent are sensitive to the ice margin and the glacial history of the region. Proglacial lakes, in turn, also impact the glacial isostatic adjustment due to loading, and ice dynamics by posing a marine-like boundary condition at the ice margin. In this study we present a tool that efficiently identifies lake basins and the corresponding maximum water level for a given ice sheet and topography reconstruction. This algorithm, called the LakeCC model, iteratively checks the whole map for a set of increasing water levels and fills isolated basins until they overflow into the ocean. We apply it to the present-day Great Lakes and the results show good agreement (∼1−4%) with measured lake volume and depth. We then apply it to two topography reconstructions of North America between the Last Glacial Maximum and the present. The model successfully reconstructs glacial lakes such as Lake Agassiz, Lake McConnell and the predecessors of the Great Lakes. LakeCC can be used to judge the quality of ice sheet reconstructions. © 2019 The Authors Journal of Quaternary Science Published by John Wiley & Sons Ltd.     

Assessment of runoff,water and sediment quality in the Selenga River basin aided by a web-based geoservice

The Selenga River is the main artery feeding Lake Baikal. It has a catchment of ~450000 km² in the boundary region between Northern Mongolia and Southern Siberia. Climate, land use and dynamic socioeconomic changes go along with rising water abstractions and contaminant loads originating from mining sites and urban wastewater. In the future, these pressures might have negative impacts on the ecosystems of Lake Baikal and the Selenga River Delta, which is an important wetland region in itself and forms the last geobiochemical barrier before the Selenga drains into Lake Baikal. The following study aims to assess current trends in hydrology and water quality in the Selenga-Baikal basin, identify their drivers and to set up models (WaterGAP3 framework and ECOMAG) for the prediction of future changes. Of particular relevance for hydrological and water quality changes in the recent past were climate and land use trends as well as contaminant influx from mining areas and urban settlements. In the near future, additional hydrological modifications due to the construction of dams and abstractions/water diversions from the Selenga’s Mongolian tributaries could lead to additional alterations.     

The geodynamic and limnological evolution of Balkan Lake Ohrid,possibly the oldest extant lake in Europe

Studies of the upper 447 m of the DEEP site sediment succession from central Lake Ohrid, Balkan Peninsula, North Macedonia and Albania provided important insights into the regional climate history and evolutionary dynamics since permanent lacustrine conditions established at 1.36 million years ago (Ma). This paper focuses on the entire 584-m-long DEEP sediment succession and a comparison to a 197-m-long sediment succession from the Pestani site ~5 km to the east in the lake, where drilling ended close to the bedrock, to unravel the earliest history of Lake Ohrid and its basin development. ²⁶Al/¹⁰Be dating of clasts from the base of the DEEP sediment succession implies that the sedimentation in the modern basin started at c. 2 Ma. Geophysical, sedimentological and micropalaeontological data allow for chronological information to be transposed from the DEEP to the Pestani succession. Fluvial conditions, slack water conditions, peat formation and/or complete desiccation prevailed at the DEEP and Pestani sites until 1.36 and 1.21 Ma, respectively, before a larger lake extended over both sites. Activation of karst aquifers to the east probably by tectonic activity and a potential existence of neighbouring Lake Prespa supported filling of Lake Ohrid. The lake deepened gradually, with a relatively constant vertical displacement rate of ~0.2 mm a⁻¹ between the central and the eastern lateral basin and with greater water depth presumably during interglacial periods. Although the dynamic environment characterized by local processes and the fragmentary chronology of the basal sediment successions from both sites hamper palaeoclimatic significance prior to the existence of a larger lake, the new data provide an unprecedented and detailed picture of the geodynamic evolution of the basin and lake that is Europe’s presumed oldest extant freshwater lake.     

Stable isotope profiles (Ca,O, C) through modern brachiopod shells of T. septentrionalis and G. vitreus: Implications for calcium isotope paleo-ocean chemistry

Brachiopod shells are widely used as an archive to reconstruct elemental and isotopic composition of seawater. Studies, focused on oxygen and carbon isotopes over the last decades, are increasingly extending to the emerging calcium isotope system. To date, only little attention has been paid to test the reliability of fossil brachiopods on their modern counterparts.In this context, the present study investigates two modern brachiopods, Terebratulina septentrionalis (eastern Canada, 5–30 m depth, 7.1 °C seasonal temperature variation, two-layer shell) and Gryphus vitreus (northern Mediterranean, 200 m depth, constant all-year round temperature, three-layer shell). Both species were sampled along the ontogenetic growth direction and calcium, oxygen, and carbon isotopes as well as elemental concentration were measured. Calcium isotopes were analyzed on TIMS. The elemental composition was analyzed by LA-ICP-MS and ICP-AES.The results indicate an intra-specimen δ^44/40Ca variation ranging from 0.16 to 0.33‰, pointing to a fairly homogenous distribution of calcium isotopes in brachiopod shells. However, in the light of the suggested 0.7‰ increase in calcium isotopes over the Phanerozoic such intra-specimen variations constrain ocean reconstruction. δ^44/40Ca values of T. septentrionalis do not seem to be affected by growth rate. Calcium isotopic values of G. vitreus are heavy in the central part of the shell and trend towards lighter values in peripheral areas approaching the maximum isotopic composition of T. septentrionalis. The maximum inter-species δ^44/40Ca difference of 0.62‰ between T. septentrionalis and G. vitreus indicates that care should be taken when using different taxa, species with different strontium content or brachiopods with specialized shell structure, such as G. vitreus, for ocean water reconstruction in terms of Ca isotopic composition. T. septentrionalis may record Ca isotopic fractionation related to seasonal seawater temperature variations in its shell but this is difficult to resolve at the current analytical precision. Average δ¹⁸O-derived temperatures of the two investigated species are close to on-site measured temperatures.     

Conceptual model for millennial climate variability: a possible combined solar-thermohaline circulation origin for the ~1,500-year cycle

Dansgaard-Oeschger and Heinrich events are the most pronounced climatic changes over the last 120,000 years. Although many of their properties were derived from climate reconstructions, the associated physical mechanisms are not yet fully understood. These events are paced by a ~1,500-year periodicity whose origin remains unclear. In a conceptual model approach, we show that this millennial variability can originate from rectification of an external (solar) forcing, and suggest that the thermohaline circulation, through a threshold response, could be the rectifier. We argue that internal threshold response of the thermohaline circulation (THC) to solar forcing is more likely to produce the observed DO cycles than amplification of weak direct ~1,500-year forcing of unknown origin, by THC. One consequence of our concept is that the millennial variability is viewed as a derived mode without physical processes on its characteristic time scale. Rather, the mode results from the linear representation in the Fourier space of nonlinearly transformed fundamental modes.     

An evaluation of the surface radiation budget over North America for a suite of regional climate models against surface station observations

Components of the surface radiation budget (SRB) [incoming shortwave radiation (ISR) and downwelling longwave radiation (DLR)] and cloud cover are assessed for three regional climate models (RCM) forced by analysed boundary conditions, over North America. We present a comparison of the mean seasonal and diurnal cycles of surface radiation between the three RCMs, and surface observations. This aids in identifying in what type of sky situation simulated surface radiation budget errors arise. We present results for total-sky conditions as well as overcast and clear-sky conditions separately. Through the analysis of normalised frequency distributions we show the impact of varying cloud cover on the simulated and observed surface radiation budget, from which we derive observed and model estimates of surface cloud radiative forcing. Surface observations are from the NOAA SURFRAD network. For all models DLR all-sky biases are significantly influenced by cloud-free radiation, cloud emissivity and cloud cover errors. Simulated cloud-free DLR exhibits a systematic negative bias during cold, dry conditions, probably due to a combination of omission of trace gas contributions to the DLR and a poor treatment of the water vapor continuum at low water vapor concentrations. Overall, models overestimate ISR all-sky in summer, which is primarily linked to an underestimate of cloud cover. Cloud-free ISR is relatively well simulated by all RCMs. We show that cloud cover and cloud-free ISR biases can often compensate to result in an accurate total-sky ISR, emphasizing the need to evaluate the individual components making up the total simulated SRB.