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521.
We have previously demonstrated that medaka CYP3A is associated with metabolism of several endobiotics including steroids and bile acids. In this study, we demonstrate that medaka CYP3A catalysis exhibits unusual kinetic behaviors consistent with allosteric interaction which cannot be described by hyperbolic kinetic models. Using 7-benzyloxy-4-(trifluoromethyl)-coumarin (BFC) and nonylphenol as CYP3A substrates, we describe both homotropic and heterotropic cooperative interactions. Given the role of CYP3A in maintaining the homeostatic balance for numerous endobiotics, enzymatic activation/inhibition by endocrine disruptors (EDCs) represents a putative (non-genomic) mechanism for endocrine disruption. 相似文献
522.
523.
The longitudinal strength of the high-speed ferry was investigated by subjecting the ship's hull girder to long-term loads obtained from a frequency-domain panel code. Prior to the statistical analysis, linearly computed transfer functions were corrected for nonlinear effects, yielding two sets of transfer functions valid for different wave amplitudes. One set corresponded to the hogging condition; the other set, to the sagging condition. Two regular equivalent design waves were specified that resulted in loads representing the most severe global design load conditions. The still-water loading condition, yielding a still-water vertical bending moment in hogging, was superimposed on the wave-induced loads to obtain the total (design) loads in hogging. For the sagging condition only, additional impact-related loads were superimposed to obtain the total (design) loads in sagging. A finite element model of the ship's structure was subjected to pressure distributions according to the two regular design waves. For comparison with classification society rule values, a simple beam theory strength analysis of the ship's midship section was performed first, and then another finite element analysis was carried out, whereby the imposed loads were tuned to the rule values of vertical bending moments. Rule-based magnitudes of nominal maximum longitudinal stress deviated significantly (25–39%) from comparable stresses obtained by the panel code based finite element analysis. However, stresses obtained from the rule-based finite element analysis agreed more favorably, especially in hogging. In the uppermost deck, for example, the panel code based compressive stress was only 9% larger than the comparable stress from the rule-based finite element analysis. 相似文献
524.
H. Jonsson K. Viken Sandnes D. Schiedek R. Schneider B. E. Grsvik A. Goksyr 《Marine environmental research》2004,58(2-5):655
In an attempt to learn more about the cytochrome P450 (CYP) system of mussels, we used protein databases and alignment software to extract highly conserved CYP sequences. From these alignments synthetic peptides were produced and used for rabbit immunisation, which yielded polyclonal antibodies against the CYP families 2 and 4. The antibodies were evaluated with Western Blot and ELISA assays, using digestive gland microsomal samples from the mussel Mytilus edulis. Western Blots revealed immunoreactions for both antibodies. The anti-CYP2 sequence rendered one major immunopositive protein of ≈49 kDa size, and weak signals for proteins of ≈41 and 56 kDa size. The anti-CYP4 sequence rendered two major bands of ≈56 and 59 kDa size, and also a weak immunoreaction with a protein of ≈43 kDa size. ELISA rendered only weak signals even with a 1:50 dilution of IgG-purified serum. A 10-day exposure to Aroclor 1254 did not appear to affect any of the immunopositive proteins, while total PCBs in soft bodies increased from 14–40 ng/g DW in controls to 373–638 ng/g DW in exposed mussels. 相似文献
525.
E. García-Luque T. A. DelValls C. Casado-Martínez J. M. Forja A. Gmez-Parra 《Marine environmental research》2004,58(2-5):671
We describe the effect of heavy metals Zn, Cd, Pb and Cu on the induction of methallothioneins on the clam Scrobicularia plana along a salinity gradient simulated under laboratory conditions. The clams were exposed to constant heavy metal concentrations in a dynamic estuary simulator during a 15-day assay to investigate possible induction of metal-binding proteins in them. The concentration of heavy metals in water was analysed. Clams were analysed for methallothionein concentrations. The speciation of Zn, Cd, Pb and Cu along the salinity gradient was modelled. Zn showed the highest concentrations and its prevalent species was the free ion. Intersite differences have been observed in methallothionein concentration and related to the salinity gradient. It seems that synthesis of methallothioneins is the result of physiological forces acting in concert with the changes in the chemical speciation of metals, owing to the trace metals uptake is controlled by means of an interaction of physiology and physicochemistry. 相似文献
526.
527.
Despite threats emanating from the influence of climate and non-climate forcing on the barrier island coastal region of southwestern Nigeria, the extent of the coastal erosion is poorly understood. We report evidence of coastal erosion and sediment accumulation in the region over a 34-year period (1973–2017), using Landsat imagery at intervals of approximately six years. Landsat image corrections and various water-extraction algorithms were used to systematically delineate coastal erosion and accumulation in the area. The region was subdivided into western and eastern subregions separated by Lagos Harbour. In the west, erosion took place during the periods 1973–1979, 1979–1984, 1990–1999 and 2005–2011, whereas in the east, erosion occurred during 1973–1979, 1990–1999 and 1999–2005. Coastal sediment accumulation occurred in the east during 1979–1984, 1984–1990, 2005–2011 and 2011–2017, whereas gains in the west occurred during 1984–1990, 1999–2005 and 2011–2017. The study revealed substantial net erosion of 1 228.1 ha in the region as a whole, over the full period. Sediment accumulation accompanying the coastal erosion appears to be linked to longshore drift. Erosion between 1973 and 2011 was probably attributable to climate change (storms and tidal conditions), longshore drift, the inflow and outflow of water at Lagos Harbour, coastal morphology and, possibly, human impacts. However, the coastal changes between 2011 and 2017 were more obviously associated with human activities, such as development of the Eko Atlantic construction project. Coastal surveillance, together with the use of environmentally sensitive protective measures, could possibly help to reduce coastal erosion in the region. Careful coastal management practices, including artificial nourishing and the installation of resilient structures (e.g. seawalls), should be undertaken to protect human settlements that are already at risk from sea-level rise. 相似文献
528.
Tiago M. Alves Vasilios Lykousis Dimitris Sakellariou Stamatina Alexandri Paraskevi Nomikou 《Geo-Marine Letters》2007,27(1):41-61
Bathymetric, 9.5-kHz long-range sidescan sonar (OKEAN), seismic reflection and sediment-core data are used in the analysis
of two tectonic troughs south of Crete, Eastern Mediterranean Sea. Here, up to 1.2 s two-way travel time (TWTT) of strata
have accumulated since the Middle Miocene in association with extension in the South Aegean region. The study area comprises
>100-km- long by >25-km-wide basins filled by sediments subdivided into two seismic units: (1) an upper Unit 1 deposited in
sub-basins which follow the present-day configuration of the southern Cretan margin; (2) a basal Unit 2, more than 500 ms
(TWTT) thick, accumulated in deeper half-graben/grabens distinct from the present-day depocentres. Both units overlap a locally
stratified Unit 3 comprising the pre-Neogene core complex of Crete and Gavdos. In this work, the interpreted seismic units
are correlated with the onshore stratigraphy, demonstrating that denudation processes occurring on Crete and Gavdos in response
to major tectonic events have been responsible for high sedimentation rates along the proximal southern Cretan margin. Consequently,
topographically confined sedimentary units have been deposited south of Crete in the last 12 Ma, including turbidites and
other mass-flow deposits fed by evolving transverse and axial channel systems. Surface processes controlling facies distribution
include the direct inflow of sediment from alluvial-fan systems and incising mountain rivers onto the Cretan slope, where
significant sediment instability processes occur at present. In this setting, seismic profiles reveal eight different types
of stratigraphic contacts on basin-margin highs, and basinal areas show evidence of halokinesis and/or fluid escape. The acquired
data also show that significant changes to the margin’s configuration occurred in association with the post-Alpine tectonic
and eustatic episodes affecting the Eastern Mediterranean. 相似文献
529.
By using the NCEP/NCAR reanalysis data sets for 1951–2001, we study the characteristics of Pacific cyclones. It is shown that the northeast-southwest
direction is predominant in the displacements of cyclones in the North Pacific. We study the variability of the field of surface
atmospheric pressure in different phases of the Pacific decadal oscillation characterizing the temperature anomalies on the
surface of the ocean in the region bounded by 20 and 60°N. It is shown that the decadal variations of the North Atlantic Oscillation
supported by the large-scale anomalies of the Pacific decadal oscillation is the most important cause of natural decadal oscillations
in the European region. We study and evaluate the regional response to the Pacific decadal oscillation by using, as an example,
the analysis of variations of the discharge of European rivers.
__________
Translated from Morskoi Gidrofizicheskii Zhurnal, No. 4, pp. 13–23, July–August, 2007. 相似文献
530.
This paper discusses the results obtained through numerical modelling of the annual course of circulation and thermohaline field evolution in the tropical Atlantic under the impact of real wind forcing (from March 1982 to March 1983) and compares them with the calculation of seasonal variability induced by the climatic wind. Changes in the pattern of the equatorial currents and related temperature fields induced by the specific peculiarities in the wind field are analysed.Translated by Vladimir A. Puchkin. 相似文献