Detection of DNA damage in mussels and sea urchins exposed to crude oil using comet assay

The single-cell microgel electrophoresis assay or the comet assay was used to evaluate DNA damage of dispersed crude oil on sea urchins (Strongylocentrotus droebachiensis) and mussels (Mytilus edulis L.). Sea urchins were exposed to 0.06 and 0.25 mg/L dispersed crude oil in a continuous flow system, while the mussels were exposed to 0.015, 0.06 and 0.25 mg/L dispersed crude oil. Sea urchin coelomocytes and mussel haemocytes were sampled after 4 and 5 weeks exposure, respectively. In the sea urchin coelomocytes, there was a significant concentration-related increase in the percentage of DNA in comet tail. In mussel haemocytes, there was a significantly higher percentage of DNA in comet tail for all treatments compared to the control. The responses were concentration-related up to 0.06 mg/L oil. The two highest exposure concentrations of mussels were not significantly different from each other. These results indicate that the comet assay can be used for biomonitoring of DNA damage in marine invertebrates following oil contamination.     

Correlative changes in metabolism and DNA damage in turbot (Scophthalmus maximus) exposed to benzo[a]pyrene

Juvenile turbot (Scophthalmus maximus) were injected intraperitoneally with either corn oil or 5 mg/kg benzo[a]pyrene (BaP) dissolved in corn oil and sampled I and 3 days after injection. After 1 day, no elevation of 7-ethoxyresorufin O-deethylase (EROD) activity was observed, however bile metabolites (BaP-7,8 dihydrodiol representing 70% of the total metabolites) and a single hepatic DNA adduct spot (0.47 adducts/10(8) nucleotides) identified by 32P-postlabelling were formed. No BaP metabolites or DNA adducts were observed in either control or carrier control fish. Fish sampled after 3 days reported 5-fold higher (P < 0.05) levels of EROD activity, a shift in the bile metabolite profile towards BaP phenol formation (1OH and 30H BaP comprising up to 60% of total metabolites detected) and the formation of two adduct spots (0.86 and 0.71 adducts/10(8) nucleotides). These results show that BaP can be metabolised and form hydrophobic DNA adducts in turbot without EROD elevation. Following EROD elevation, a shift in the profile of both BaP metabolites and BaP metabolite-DNA interactions occurs indicative of other oxidative processes.     

Microbial Biomass and Organic Nutrients in the Deep-Sea Sediments of the Central Indian Ocean Basin

In order to assess the impact of deep-sea mining on the in situ benthic life, we measured the microbial standing stock and concentration of organic nutrients in the deep-sea sediments of the Central Indian Ocean Basin in the Indian pioneer area. Sediments were collected using box core and grab samples during September 1996. The total bacterial numbers ranged from 10 10 -10 11 cells per g -1 dry weight sediment. There was a marginal decrease in the number of bacteria from surface to 30 cm depth, though the subsurface section registered a higher number than did the surface. The highest numbers were encountered at depths of 4-8 cm. The retrievable number of bacteria were two orders less in comparison with the direct total counts of bacteria. An almost homogeneous distribution of bacteria, total organic carbon, living biomass, and lipids throughout the depth of cores indicates active microbial and benthic processes in the deep sea sediments. On the other hand, a uniform distribution of total counts of bacteria, carbohydrates, and total organic carbon in all the cores indicates their stable nature and suggests that they can serve as useful parameters for long-term monitoring of the area after the benthic disturbance. Further studies on temporal variability in this region would not only verify the observed norms of distribution of these variables but would also help to understand restabilization processes after the simulated benthic disturbance.     

Acoustic imaging,visualization, and quantification of buoyant hydrothermal plumes in the Ocean

We develop and apply visualization and quantification methods to reconstruct hydrothermal plumes in 3D from acoustic images and to make the first direct measurements from the reconstructions of scalar properties that describe the behavior of two buoyant plumes discharging from adjacent black smoker chimneys. The actual behavior is then compared to that predicted by a classic simple buoyant plume model. The images are reconstructed as isointensity surfaces of backscatter from particulate matter suspended in the plumes. The measurements pertinent to the role of the plumes as agents of dispersal of heat and mass into the ocean include change with height of diameter, particle distribution, dilution, centerline attitude, surface protrusions, and connectivity. The protrusions are the surface expression of eddies and appear to follow a bifurcating helical flow pattern that resemble simulation of the naturally forced flow of coherent vortex rings as the eddies rise with the buoyant plume. These direct measurements and the derived entrainment coefficient are generally consistent with behavior predicted by the simple buoyant plume model and support engulfment by vortex shedding as a primary mechanism for entrainment of surrounding seawater. Deviations from predicted buoyant plume behavior are diagnostic of particle dynamics.     

Vertical Sound Velocity Transition in the Coral Reef Core and Its Significance of Indicating Facies

On the basis of the sound velocity measurements of the coral reef core from Nanyong No.1 well of Yongshu Reef in the Nansha Islands,the paper studies the relations between the vertical sound velocity transition features in the coral reef core and the corresponding stratigraphic depositional facies change as well as stratigraphic gap of erosion,analyses the cause of the sound velocity transition,expounds the concrete process of the sea level change resulting in the stratigraphic gap of erosion and facies change in the coral reef and explains the relations between the vertical sound velocity transition in the coral reef core and the corresponding stratigraphic paleoclimate and the sea level change.This study is of important practical value and theoretical significance to the island and reef engineering construction and the acoustic logging for oil exploration in the reef limestone area as well as the paleoceanographic study of the marginal sea in the westerm Pacific Ocean.     

A singular evolutive extended Kalman filter to assimilate ocean color data in a coupled physical–biochemical model of the North Atlantic ocean

Within the European DIADEM project, a data assimilation system for coupled ocean circulation and marine ecosystem models has been implemented for the North Atlantic and the Nordic Seas. One objective of this project is to demonstrate the relevance of sophisticated methods to assimilate satellite data such as altimetry, surface temperature and ocean color, into realistic ocean models. In this paper, the singular evolutive extended Kalman (SEEK) filter, which is an advanced assimilation scheme where three-dimensional, multivariate error statistics are taken into account, is used to assimilate ocean color data into the biological component of the coupled system. The marine ecosystem model, derived from the FDM model [J. Mar. Res. 48 (1990) 591], includes 11 nitrogen and carbon compartments and describes the synthesis of organic matter in the euphotic zone, its consumption by animals of upper trophic levels, and the recycling of detritic material in the deep ocean. The circulation model coupled to the ecosystem is the Miami isopycnic coordinate ocean model (MICOM), which covers the Atlantic and the Arctic Oceans with an enhanced resolution in the North Atlantic basin. The model is forced with realistic ECMWF ocean/atmosphere fluxes, which permits to resolve the seasonal variability of the circulation and mixed layer properties. In the twin assimimation experiments reported here, the predictions of the coupled model are corrected every 10 days using pseudo-measurements of surface phytoplankton as a substitute to chlorophyll concentrations measured from space. The diagnostics of these experiments indicate that the assimilation is feasible with a reduced-order Kalman filter of small rank (of order 10) as long as a sufficiently good identification of the error structure is available. In addition, the control of non-observed quantities such as zooplankton and nitrate concentrations is made possible, owing to the multivariate nature of the analysis scheme. However, a too severe truncation of the error sub-space downgrades the propagation of surface information below the mixed layer. The reduction of the actual state vector to the surface layers is therefore investigated to improve the estimation process in the perspective of sea-viewing wide field-of-view sensor (SeaWiFS) data assimilation experiments.     

Propagation of the Southwest Indian Ridge at the Rodrigues Triple Junction

The analysis of multibeam bathymetric data of the Southwest Indian Ridge(SWIR) domain between the triple junction traces from 68° E to theRodrigues Triple Junction (RTJ; 70° E) reveals the evolution of thisridge since magnetic anomaly 4 (8 Ma). Image processing has been used toshow that the horizontal component of strain due to a network of normal stepfaults increases dramatically between 69°30 E and the RTJ. Thisarea close to the RTJ is characterized by a deep graben at the foot of thetriple junction trace on the African plate and by a narrow fault-boundedridge that joins an offset of the trace on the Antarctic plate. In thatarea, spreading is primarily amagmatic and dominated by tectonic extensionprocesses. To the west of 69°30 E, some lobate bathymetricfeatures atop of a large topographic high suggest volcanic constructions.Between 68°10 E and 69°25 E the southern flank of theSWIR domain is wider than the northern one and is characterized by a series of 7 en echelon bathymetric highs similar in size,shape and orientation to the one centred at 69°30E near the present-day triple junction. Their en echelon organization along the triple junction trace on the Antarctic plate and the typical lack of conjugated parts on the northern flank show that these bathymetric highs have been shifted to the south by successive northward relocalisations of the SWIR rifting zone. This evolution results in the asymmetric spreading of the SWIR in the survey area. The off-axis bathymetric highs connect to the offsets of the triple junction trace on the Antarctic plate when the Southeast Indian Ridges lightly lengthenstoward the northwest and the triple junction is relocated to the north. We propose that the SWIR lengthens toward the northeast with two propagation modes: 1) a continuous and progressive propagation with distributed deformation in preexisting crust of the Central Indian Ridge, 2) a discontinuous propagation with focusing of the deformation in a rift zone when the triple junction migrates rapidly to the north. The modes of propagation of the SWIR are related to different localisation and distribution of strain which are in turn controlled by changes of the triple junction configurations due to propagation, recession or a symmetric spreading on the Central and Southeast Indian Ridges.     

厦门地区软相潮间带多毛类的生态

根据厦门地区１９９０年２月至１９９２年１月调查软相潮间带所获资料，研究了该区多毛类的种类组成，数量与分布，结果表明，该区多毛类种类丰富，共鉴定出１１８种，隶属于３７科８３属。其中，高潮区４０种，中潮区９０种，低潮区８３种。多毛类平均栖息密度８７个／ｍ＾３，平均生物量３．３３ｇ／ｍ＾２。