Concurrent imaging of chlorophyll fluorescence,Chlorophyll a content and green fluorescent proteins‐like proteins of symbiotic cnidarians

Research on photosynthetic cnidarians has been mainly focused on the symbiosis established between the cnidarian host and its dinoflagellates endosymbionts from genus Symbiodinium. Despite the potential of imaging techniques for assessing the spatial distribution of key parameters of cnidarian photobiology, such as photochemical activity, chlorophyll a content or green fluorescent proteins (GFPs), to our best knowledge, no study has ever attempted to simultaneous map these three features. In this study, we developed a modified imaging pulse amplitude fluorometer by applying excitation light of different wavelengths and selectively detecting short spectral bands through bandpass filters. The imaging system was used to sequentially excite and quantify chlorophyll variable fluorescence (maximum quantum yield of photosystem II, F_v/F_m), Chl a content (normalized difference vegetation index) and relative content of GFPs. The spatial distribution of these photophysiological parameters was mapped both horizontally, across the surface of the soft corals Sarcophyton cf. glaucum and Sinularia flexibilis and the zoanthid Protopalythoa sp., and vertically, throughout a vertical section of S. cf. glaucum. Results showed bleached areas within each individual coral colony and registered photophysiological changes with S. cf. glaucum tissue depth. Analysis of Protopalythoa sp. polyps’ expansion revealed differential surface patterns of NDVI and GFP concentration, and a negative relation between these latter parameters within each polyp. This novel non‐invasive approach allowed a high‐resolution characterization of the spatial relationship between these key parameters through the analysis of image information on a pixel‐by‐pixel basis, which has great potential for investigating the physiological state of symbiotic associations.     

Sequential extraction and leaching characteristics of heavy metals in abandoned tungsten mine tailings sediments

The chemical speciation of potentially toxic elements (As, Cd, Cu, Pb, and Zn) in the contaminated soils and sulfides-rich tailings sediments of an abandoned tungsten mine in Korea was evaluated by conducting modified BCR sequential extraction tests. Kinetic and static batch leaching tests were also conducted to evaluate the potential release of As and other heavy metals by acidic rain water and the leaching behaviors of these heavy metals. The major sources of the elements were As-, Zn- and Pb-bearing sulfides, Pb carbonates (i.e., cerussite), and Pb sulfates (i.e., anglesite). The biggest pollutant fraction in these soil and tailing samples consists of metals bound to the oxidizable host phase, which can be released into the environment if conditions become oxidative, and/or to residual fractions. No significant difference in total element concentrations was observed between the tailings sediments and contaminated soils. For both sample types, almost no changes occurred in the mobility of As and the other heavy metals at 7 days, but the mobility increased afterwards until the end of the tests at 30 days, regardless of the initial pH. However, the mobility was approximately 5–10 times higher at initial pH 1.0 than at initial pHs of 3.0 and 5.0. The leached amounts of all the heavy metal contents were higher from tailings sediments than from contaminated soils at pH > 3.0, but were lower at pH < 3.0 except for As. Results of this study suggest that further dissolution of heavy metals from soil and tailing samples may occur during extended rainfall, resulting in a serious threat to surface and groundwater in the mine area.     

Geophysical methods applied to fault characterization and earthquake potential assessment in the Lower Tagus Valley, Portugal

The study region is located in the Lower Tagus Valley, central Portugal, and includes a large portion of the densely populated area of Lisbon. It is characterized by a moderate seismicity with a diffuse pattern, with historical earthquakes causing many casualties, serious damage and economic losses. Occurrence of earthquakes in the area indicates the presence of seismogenic structures at depth that are deficiently known due to a thick Cenozoic sedimentary cover. The hidden character of many of the faults in the Lower Tagus Valley requires the use of indirect methodologies for their study. This paper focuses on the application of high-resolution seismic reflection method for the detection of near-surface faulting on two major tectonic structures that are hidden under the recent alluvial cover of the Tagus Valley, and that have been recognized on deep oil-industry seismic reflection profiles and/or inferred from the surface geology. These are a WNW–ESE-trending fault zone located within the Lower Tagus Cenozoic basin, across the Tagus River estuary (Porto Alto fault), and a NNE–SSW-trending reverse fault zone that borders the Cenozoic Basin at the W (Vila Franca de Xira–Lisbon fault). Vertical electrical soundings were also acquired over the seismic profiles and the refraction interpretation of the reflection data was carried out. According to the interpretation of the collected data, a complex fault pattern disrupts the near surface (first 400 m) at Porto Alto, affecting the Upper Neogene and (at least for one fault) the Quaternary, with a normal offset component. The consistency with the previous oil-industry profiles interpretation supports the location and geometry of this fault zone. Concerning the second structure, two major faults were detected north of Vila Franca de Xira, supporting the extension of the Vila Franca de Xira–Lisbon fault zone northwards. One of these faults presents a reverse geometry apparently displacing Holocene alluvium. Vertical offsets of the Holocene sediments detected in the studied geophysical data of Porto Alto and Vila Franca de Xira–Lisbon faults imply minimum slip rates of 0.15–0.30 mm/year, three times larger than previously inferred for active faults in the Lower Tagus Valley and maximum estimates of average return periods of 2000–5000 years for M 6.5–7 co-seismic ruptures.     

Biogeochemistry of the Madeira river basin

A biogeochemical characterization of the Madeira river basin has been made to evaluate the local and global effects of possible alterations in the ecosystem caused by recent intensive occupation in Rondonia state. During the period April 1983—January 1986, sampling was made both by land and river along the tributaries and main channel of the Madeira river. The parameters analysed lead to a detailed study of the physicochemical quality of the waters of the basin and their relationship with the local geology, associated with the transport of solid material and the hydrological behavior of the ecosystem.Penman's method adapted to tropical rainforest conditions was used to evaluate the potential evapotranspiration for the basin. Estimated potential evapotranspiration was 1420 mm/y, 77% due to the energy balance. Real evapotranspiration was 94% of the estimated potential and the main residence time of the rain water in the basin was 2 months. The isotopic behavior of Hydrogen and Oxygen in the river waters of the region was typical of great rivers, the values being more positive during the dry season and more negative during the rainy season. An isotopic gradient of ¹⁸O 0.038 ()/100 km, was established from Porto Velho station to the estuary, which was considered low when compared with the value of 0.063 ()/100 km, obtained for the Amazon river.In general, the waters of the tributaries were poor in dissolved ion species when compared with the main channel of the Madeira river. Seasonal variation in the transport of suspended sediment kept the same pattern, greater transport being observed on rising water than during high water. A transport of 2.85 million tons per day was observed in the Madeira river near the mouth.     

Sequence hierarchy in a Mesoproterozoic interior sag basin: from basin fill to reservoir scale,the Tombador Formation,Chapada Diamantina Basin,Brazil

The Tombador Formation exhibits depositional sequence boundaries placed at the base of extensive amalgamated fluvial sand sheets or at the base of alluvial fan conglomeratic successions that indicate basinward shifts of facies. The hierarchy system that applies to the Tombador Formation includes sequences of different orders, which are defined as follows: sequences associated with a particular tectonic setting are designated as ‘first order’ and are separated by first‐order sequence boundaries where changes in the tectonic setting are recorded; second‐order sequences represent the major subdivisions of a first‐order sequence and reflect cycles of change in stratal stacking pattern observed at 10² m scales (i.e., 200–300 m); changes in stratal stacking pattern at 10¹ m scales indicate third‐order sequences (i.e., 40–70 m); and changes in stratal stacking pattern at 10⁰ m scales are assigned to the fourth order (i.e., 8–12 m). Changes in palaeogeography due to relative sea level changes are recorded at all hierarchical levels, with a magnitude that increases with the hierarchical rank. Thus, the Tombador Formation corresponds to one‐first‐order sequence, representing a distinct intracratonic sag basin fill in the polycyclic history of the Espinhaço Supergroup in Chapada Diamantina Basin. An angular unconformity separates fluvial‐estuarine to alluvial fan deposits and marks the second‐order boundary. Below the angular unconformity the third‐order sequences record fluvial to estuarine deposition. In contrast, above the angular unconformity these sequences exhibit continental alluvial successions composed conglomerates overlain by fluvial and eolian strata. Fourth‐order sequences are recognized within third‐order transgressive systems tract, and they exhibit distinct facies associations depending on their occurrence at estuarine or fluvial domains. At the estuarine domain, they are composed of tidal channel, tidal bar and overlying shoreface heterolithic strata. At the fluvial domain the sequences are formed of fluvial deposits bounded by fine‐grained or tidal influenced intervals. Fine grained intervals are the most reliable to map in fourth‐order sequences because of their broad laterally extensive sheet‐like external geometry. Therefore, they constitute fourth‐order sequence boundaries that, at the reservoir approach, constitute the most important horizontal heterogeneity and, hence, the preferable boundaries of production zones. The criteria applied to assign sequence hierarchies in the Tombador Formation are based on rock attributes, are easy to apply, and can be used as a baseline for the study of sequence stratigraphy in Precambrian and Phanerozoic basins placed in similar tectonic settings.     

Probabilistic earthquake risk assessment using CAPRA: application to the city of Barcelona, Spain

The risk evaluation model CAPRA (Comprehensive Approach to Probabilistic Risk Assessment) is a techno-scientific methodology and information platform, composed of tools for evaluating and communicating risk at various territorial levels. The model allows evaluating losses on exposed elements using probabilistic metrics, such as the loss exceedance curve, the expected annual loss and the probable maximum loss, useful for multi-hazard risk analyses. In this article, the process of probabilistic seismic risk analysis is described, explaining the main features of the CAPRA modules of hazard, vulnerability and risk estimation applied to the city of Barcelona, Spain. In addition, according to the physical risk results and the information on the socioeconomic indicators of the city, this article presents the holistic evaluation of seismic risk, which is a valuable result to facilitate the integrated risk management by the different stakeholders involved in risk reduction decision making.     

Metallic Mineral Resources in the Twenty-First Century. I. Historical Extraction Trends and Expected Demand

Industrial, technological, and economic developments depend on the availability of metallic raw materials. As a greater fraction of the Earth’s population has become part of developed economies and as developed societies have become more affluent, the demand on metallic mineral resources has increased. Yet metallic minerals are non-renewable natural resources, the supply of which, even if unknown and potentially large, is finite. An analysis of historical extraction trends for eighteen metals, going back to the year 1900, demonstrates that demand of metallic raw materials has increased as a result of both increase in world population and increase in per-capita consumption. These eighteen metals can be arranged into four distinct groups, for each of which it is possible to identify a consistent pattern of per-capita demand as a function of time. These patterns can, in turn, be explained in terms of the industrial and technological applications, and in some cases conventional uses as well, of the metals in each group. Under the assumption that these patterns will continue into the future, and that world population will grow by no more than about 50% by the year 2100, one can estimate the amount of metallic raw materials that will be required to sustain the world’s economy throughout the twenty-first century. From the present until the year 2100, the world can be expected to require about one order of magnitude more metal than the total amount of metal that fueled technological and economic growth between the age of steam and the present day. For most of the metals considered here, this corresponds to 5–10 times the amount of metal contained in proven ore reserves. The two chief driving factors of this expected demand are growth in per-capita consumption and present-day absolute population numbers. World population is already so large that additional population growth makes only a small contribution to the expected future demand of metallic raw materials. It is not known whether or not the amount of metal required to sustain the world’s economy throughout this century exists in exploitable mineral resources. In the accompanying paper, I show that it is nevertheless possible to make statistical inferences about the size distribution of the mineral deposits that will need to be discovered and developed in order to satisfy the expected demand. Those results neither prove nor disprove that the needed resources exist but can be used to improve our understanding of the challenges facing future supply of metallic raw materials.