Types and characters of sub-bottom acoustic reflection in eastern part of Bohai Sea

Ｈｉｇｈ－ｒｅｓｏｌｕｔｉｏｎｓｕｂ－ｂｏｔｔｏｍｐｒｏｆｉｌｅｒｈａｓｒｅｖｅｄｅｄｓｅｄｉｍｅｎｔａｌｓｔｒａｔａａｎｄｅｎｔｉｒｏｎｍｅｎｔｓｉｎｔｈｅｅｕｔｔｍｐ．ｏｆｔｈｅｌ３ｏｈａｉＳｅａｓｉｎｃｅｔｈｅｌｔａｔｅＰｌｅｉｓｔｏｃｅｎｅ．Ａｔｃｃｏｒｄｉｎｇｔｏｔｈｅｃｈａｒａｃｔｅｒｓｏｆａｃａｕｓｅｉｃｒｅｆｌｅｃｔｉｏｎａｎｄｓｅｉｓｍｉｃｔｓｔｒａｔｔｌｇｍｐｈｉｃｐｒｉｎｃｉ－ｐｌｅ，ｓｏｍｅｃｌｔ．ｉｆｉｃａｔｉｏｎｏｆｔｙｐｉｃａｌａｃｏｕｓｔｉｃｒｅｆｌｅｃｔｉｏｎｉｓｎｚａｄｅｏｕｔｔｎｄｗｉＵｂｅｆａｖｏｕｒａｂｌｅｆｏｒｉｎｔｅｒｐｒｅｔａｔｉｏｎａｎｄｒｅｃｏｇｎｂａｔｉｏｎｏｆａｏｏｕｓｔｉｃｐｒｏｆｉｌｅｓｉｎｔｈｉｓａｒｅａ     

利用牙鲆鳃细胞系分离和培养淋巴囊肿病毒

本文利用牙鲆鳃细胞系进行了养殖牙鲆淋巴囊肿病毒的分离及培养 ,并通过电镜对培养细胞中淋巴囊肿病毒的形态及感染循环进行了初步研究。将病鱼的淋巴囊肿组织无菌滤液接种牙鲆细胞系 ,细胞出现了明显的细胞病变 ( Cytopathic effect,CPE)。电镜观察在培养细胞的胞质中有病毒的包涵体 ,胞质中散在 6角形、5角形或圆形的病毒粒子 ,大小为 10 0～ 140 nm之间。在感染细胞的线粒体中也存在大量的病毒颗粒。     

Diagnosis of iridovirus in large yellow croaker by PCR

A rapid and sensitive PCR-based method for the detection of the large yellow croaker iridovirus(LYCIV) is described,which involves the amplification of a 295 bp fragment of the LYCIV ATPase gene from DNA isolated from naturally infected fish spleen.Sequencing of LYCIV ATPase gene fragment showed it shared 100% nucleotide sequence homology with the corresponding region of the ATPase gene of red sea bream iridovirus(RSIV) and sea bass iridovirus(SBIV),suggesting that LYCIV was homologous with RSIV and SBIV at least in part of the gemone.The specificity and sensitivity of the PCR procedure were tested on the iridovirus-infected fishes,the expected fragment was detected from spleen DNA samples of infected fishes,whereas no fragments were amplified from healthy fish spleen DNA,white spot syndrome baculoviruses(WSBV) DNA and pseudorabies virus(PRV) DNA.Detection limit of this method was 10^-7 ng positive plasmid DNA containing target sequence,equal to about 100 virions.In the infected experiment,first positive detection(1/4) appeared at Day 3 post-infection,all fish(4/4) tested positive at Day 7,however obvious symptoms were observed at Day 8,so LYCIV infection could be detected prior to the appearance of obvious symptoms.These results indicate that this PCR method could be used for early,rapid and specific detection of LYCIV infection.     

冲绳海槽中西部边缘区沉积物成因和沉积环境

本文根据１９９６年“中－法合作东海计划”在冲绳海槽中西部边缘区所取柱状样岩芯沉积物粒度分析资料,对该区沉积物特征进行研究。结果表明,火山沉积和富含生物的半远洋深海软泥沉积是本区的主要特征。以４８１ｃｍ为界,上、下两层沉积物截然不同,可能是沉积物的来源不同或者是水动力条件的差异。该区主要受火山活动的影响,其次是黑潮、大洋风暴     

影响萼花臂尾轮虫种群增长的生态学因子的研究：温度和饵料密度的影响

本文研究了在5个温度梯度和4个饵料密度的20个试验组合下，萼化臂尾轮虫（Brachionuscalyciflorus）的种群增长规律。结果表明，该轮虫实验种群的最适温度范围是28—32℃，临界高温是36－40℃；最适饵料密度是45－65×106个／ml。这两个生态因子的最佳组合是温度32℃，饵料密度为65×106个／ml，此时，种群达最大密度3．4×103个／ml。     

一个湍能封闭模型对风暴潮海底底应力的应用研究

将一个三维湍能封闭模型应用于开阔海区的风暴潮，通过数值计算探讨了Ｔａｙｌｏｒ底摩擦二次率的拖曳系数随空间的分布及拖曳系数与水深、海底粗糙度、风向和风速等因素的关系。本文对底摩擦二次率的可靠性做了评价。     

A new type of Shipboard Meteorological Satellite Receiving-processing System

INTRODUCTIONTheSMSRPSisstalledontheshipnavigatingovertheseaandreceivessatellitecloudmapsatanytimetoprovidereliablereal-timedataofmeteorologyandocean.Itisanimportantequipmentforsafeguardofshipnavigation.Chinahasthousandsofoceanicships.Butalmostallships,withonlyveryfewexception,arenotstalledbySMSRPS.Thecausesareasfollows:1.Thetechniqueisverycomplicated.2.Thedevelopmentcostisveryhigh.3.Ifweintroduceforeignequipment,thecostistooexpensive.4.Foreignequipmentanditssoftwarearecompletelyclo…     

大生活用海水生化处理技术研究进展

采用海水作为大生活用水最结解沿海城市淡水资源紧缺局面的有效途径之一。该技术得以推广应用的技术关键是大生活用海水的后处理技术，文章综述了大生活用海水的生化处理技术的研究现状，并提出了今后的研究方向。     

沉降监测多项式回归分析与神经网络预测

使用多项式和切比雪夫(Tchebyshev)多项式分别对沉降监测数据进行回归分析以预测未来沉降值,其中切比雪夫多项式的外推效果较好;应用前向BP神经网络对两种不同的单因子输入模式进行非线性函数逼近,并进行了不同采样步长的比较,实例表明将时间点作为网络的输入对沉降进行预测效果较好。     

碳水化合物的组合合成

碳水化合物的组合合成是一项新兴技术，该技术可以在短时间内合成大量用于进行生物活性筛选的寡糖及拟糖物。液相与固相合成技术可以极大地加快药物研究与开发进程。作者针对该技术在碳水化合物合成方面的研究进展情况进行讨论。