象山港养殖大黄鱼寄生新贝尼登虫成虫形态学和28S rDNA,ITS1分子鉴定

贝尼登类单殖吸虫是象山港海水养殖鱼类中一类危害严重的寄生虫。应用PCR扩增及DNA序列分析的分子生物学手段,并结合对成虫的形态学分析,对象山港养殖大黄鱼体表寄生的贝尼登类单殖吸虫(记作XSp)进行了种类鉴定,结果表明XSp从形态特征上属于新贝尼登虫属,与梅氏新贝尼登虫高度相似。扩增得到XSp的28S rDNA和ITS1序列长度分别为393和427 bp,与梅氏新贝尼登虫和鱾新贝尼登虫的5个28S rDNA 序列、2个ITS1序列的比对分析显示相似性除1个为97.4%外其余均大于99%,提示XSp与这几个鱾新贝尼登虫和梅氏新贝尼登虫为种内关系,而XSp与贝尼登虫的3个28S rDNA 和1个ITS1序列相似性分别为84.3%~89.5%和60.2%。系统进化树显示该吸虫与梅氏新贝尼登虫和鱾新贝尼登虫形成一个紧密的簇,而与贝尼登虫亲缘关系较远。根据普通生物学和序列特征分析,将XSp定种为梅氏新贝尼登虫,并且支持Whittington和Horton (1996)提出的梅氏新贝尼登虫和鱾新贝尼登虫为同种异名的分类观点。     

胶州湾大沽河口潮间带沉积物中叶绿素和有机质含量的季节变化

为了解胶州湾大沽河口潮间带沉积物中底栖微藻和有机质含量的季节变化,于2010年4月至2011年2月,分4次(春、夏、秋、冬)测定分析了沉积物中的叶绿素含量和有机质含量,并分析了沉积物的粒度组成及现场温度。结果表明,表层(0～2cm)沉积物叶绿素a含量为0.42～6.06mg/kg,有机质含量为0.48%～3.71%;次表层(2～5cm)叶绿素a含量为0.13～3.53mg/kg,有机碳质量为0.31%～3.78%;深层(5～8cm)叶绿素a含量为0.14～1.95mg/kg,有机质含量为0.47%～3.72%。经统计检验和Pearson相关性分析得出,胶州湾大沽河口沉积物中的叶绿素a含量春冬两季最高,夏季和秋季最低;叶绿素a含量与深度和温度呈负相关;与所处潮带、有机质含量、粉砂黏土含量在本实验中均无显著的相关性;有机质含量与季节关系不大,高潮带最高,其含量与粉砂黏土含量呈正相关,与叶绿素含量、深度和温度关系不明显。     

鉴定海参的斑点杂交法及其应用

本研究目的是通过斑点杂交方法对不同种类的海参进行快速准确的鉴定。采用CTAB沉淀法提取海参的DNA,PCR扩增线粒体COⅠ基因并测序,利用Primer premier 5.0软件设计并筛选了仿刺参(Apostichopus japonicus)、北大西洋瓜参(Cucumaria frondosa)、加州红参(Parastichopus californicus)及梅花参(Thelenota ananas)4种海参的特异性探针,设计并进行斑点杂交实验。实验结果显示:4条探针均具有高度的特异性,灵敏度可达100pg,能够实现对仿刺参、北大西洋瓜参、加州红参和梅花参的准确鉴定。本文建立的斑点杂交方法,为海参品种的快速、准确的鉴定提供了可靠的技术方法。     

Estimation of the minimum food requirement using the respiration rate of medusa of Aurelia aurita in Sihwa Lake

We examined the respiration rate of Aurelia aurita medusae at 20 °C and 28 °C to evaluate minimum metabolic demands of medusae population in Sihwa Lake, Korea during summer. While weight specific respiration rates of medusae were constant and irrespective to the wet weight (8?C220 g), they significantly varied in respect to temperatures (p<0.001, 0.11±0.03 mg C g^?1 of medusa d^?1 at 20°C and 0.28±0.11 mg C g^?1 of medusa d^?1 at 28 °C in average, where Q ₁₀ value was 2.62). The respiration rate of medusae was defined as a function of temperature (T, °C) and body weight (W, g) according to the equation, R=0.13×2.62^(T-20)/10 W ^0.93. Population minimum food requirement (PMFR) was estimated from the respiration rate as 15.06 and 4.86 mg C m^?3 d^?1 in June and July, respectively. During this period, increase in bell diameter and wet weight was not significant (p=1 in the both), suggesting that the estimated PMFR closely represented the actual food consumption in the field. From July to August, medusae grew significantly at 0.052 d-1, thus the amount of food ingested by medusae population in situ was likely to exceed the PMFR (1.27 mg C m^?3 d^?1) during the period. In conclusion, the medusae population of higher density during June and July had limited amount of food, while those of lower in July and August ingested enough food for growth.     

Karst cave tourism system in Zhejiang Province based on resource regional analysis

Karst cave tourism plays an important role in the overall tourism of Zhejiang Province,China.In analyzing the current status of karst cave tourism resources,it is crucial to develop a scientific system for optimizing resource exploitation and tourism development in the future.This study conducted an analysis of resource characteristics and regional structure in Zhejiang Province.Nearest neighbor index(NNI) method and accessibility index method were used for a comprehensive understanding of the effects of scale,strength,combination,and accessibility of karst cave tourism resources.Results indicated that karst cave tourism resources in Zhejiang Province have a significant regional influence,and that resource quality and exploitation are diverse in different regions.Among the regions,Jinhua had the highest exploitation proportion of over 60% and the lowest NNI value of 0.098.Furthermore,regional analysis inferred that different karst caves demonstrate diversity in accessibility to tourism markets,among which the Lingshan Cave,Fengshui Cave,and Xianqiao Cave reveal the highest accessibility index of 2.41.Finally,we put forward a karst cave tourism system in Zhejiang Province based on the Growth Pole Theory and set up an overall scheme for karst cave tourism development.From a regional perspective analysis,the study refined the methods for regional resource research and provided a strategic proposal for karst cave tourism in Zhejiang Province.     

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基于分形维度法的都市区城市蔓延分析——以广佛为例

根据1957与1982年两个时期的地形图并结合1995与2008年的遥感卫星影像提取城镇建设用地专题信息,利用ArcGIS建立广佛都市区城镇建设用地数据库,采用分形维度的计算方法,对广佛都市区城市蔓延的情况进行测度和分析。结果表明广佛都市区三个发展阶段城镇建设用地面积增长速度并不均匀,具有加速发展的特点。四个测度年份的分形维数分别为1.645 4,1.628 5,1.558 6和1.527 0,均在1～2之间,分形维数呈递降趋势,总体下降了0.118 4,城市发展模式由紧凑型逐渐向松散型演变,存在城市蔓延情况,而且城市发展模式以年均0.14%的速度从紧凑型向松散型演变。广佛都市区城市蔓延的地域分异较为明显,具有较强的空间集聚性和中心向心性,广州和佛山毗邻地区以及中心城区是城市蔓延的活跃区和集中区,1982-1995年间研究区内蔓延速度比1995-2008年间要快。通过深入分析发现,经济快速发展、城市人口的持续增长、交通道路网络的建设是广佛都市区快速扩张和城市蔓延的基本动力因素之一,开发区土地扩张,进一步加剧了无序扩张和城市蔓延。     

考虑裂缝干扰的气藏压裂水平井产能预测模型

基于压裂水平井井身结构复杂,需要建立考虑裂缝参数变化且符合水平井渗流特征的产能预测模型.为准确预测压裂水平井产能,通过保角变换和势的叠加原理,建立新模型,不仅考虑裂缝长度、裂缝间距、裂缝夹角的变化对压裂水平井产能的影响,还考虑裂缝间的相互干扰.结果表明:压裂水平井产能随着裂缝条数、长度和裂缝夹角的增加而增大.模型通过现场验证,计算单井无阻流量与试采结果对比,相对误差为8.3%,计算结果较为准确.     

城市热岛效应下浅层土中混凝土的酸腐蚀试验研究

针对城市热岛效应引起城区浅层土地温场升高这一观测结果,采用室内快速模拟试验方法,对城区酸性土壤腐蚀混凝土材料的温度效应开展了试验研究。试验研究了在温度为5℃、20℃和40℃条件下,混凝土试样在酸浓度分别为0%、5%和10%的沙土介质中放置30d、90d后的抗压强度变化规律,并对混凝土试样在腐蚀过程中的腐蚀系数变化规律和微观机理进行了分析;最后,对南京城区热岛效应环境下浅层土中混凝土材料酸腐蚀的强度变化规律进行了分析。试验结果表明,在同一腐蚀浓度下,混凝土试样抗压强度的下降速度随着温度的升高而不断加快,其腐蚀系数也随着温度的升高而不断增大;腐蚀介质的浓度越大,环境温度对混凝土腐蚀系数的影响就越明显;在温度为40℃、硫酸浓度为10%的沙土中放置30 d后,混凝土的腐蚀系数K达到45.21%,约是在5℃条件下腐蚀系数的2倍,相当于环境温度每上升1℃,混凝土腐蚀系数平均增加0.64%。该研究成果对于减轻城市热岛效应对岩土工程的影响具有重要意义。