白斑综合症杆状病毒在养殖万氏对虾(Penaeus vannamei)中暴发流行和人工感染研究

白斑综合症杆状病毒(White spot syndrome baculovirus,WSBV)对万氏对虾有较强的致病力,在病虾被感染的2～3d内,就可以使万氏对虾死亡率达83.3%～90%.WSBV对万氏对虾的鳃上皮细胞、甲壳下表皮细胞、胃上皮组织细胞、肌细胞、结缔组织细胞和中肠基底膜下肌细胞以及结缔组织细胞敏感.在细胞质中的完整杆状病毒粒子大小为(129～164)nm×(365～412)nm,在细胞核中的完整杆状病毒粒子大小约为(135～176)nm×(429～480)nm.万氏对虾对wSBv没有明显的抗病性,60d养殖死亡率达90%.     

北祁连山白山子花岗闪长岩成岩时代

寒山大型金矿是近年来在北祁连山西段发现的与侵人岩有关的构造蚀变岩型金矿。笔者首次利用锆石U-Pb方法测得白山子花岗闪长岩的形成年龄为370±25 Ma，属于华力西期。寒山金矿的成矿主要在213.95~339 Ma间。在多期热液叠加，多期成矿作用中，早期成矿的热液很可能是白山子花岗闪长岩（370±25 Ma) ,寒山辉长岩(347.1±6. 4 Ma）共同提供的。由于该区有较多的中酸性岩体存在，它们可能为金的主要来源，因而，确定这些侵入体的形成年龄，对于在该区寻找蚀变岩型金矿不仅有重要的理论意义，而且有重要的现实意义。     

安徽铜陵新桥Cu-Au-Fe-S矿床黄铁矿Re-Os定年及成矿意义

安徽铜陵地区是长江中下游Cu-Au-Fe-S成矿带中的一个重要成矿区,新桥大型Cu-Au-Fe-S矿床为其重要的矿床之一.运用Re-Os同位素定年方法对该矿床中矿石的黄铁矿进行了精确定年测定,首次获得了黄铁矿的Re-Os等时线年龄为126±11Ma,初始OS值1.2±1.9（MSWD=2.6）,属早白垩世,代表了铁硫化物的成矿年龄.结合前人的精确同位素定年结果,认为铜陵地区存在两种成矿事件,一种是与Cu-Au-Mo矿有关的成矿事件,代表的铜钼金硫化物的成矿年龄为138.0～140.0 Ma;另一种与铁矿有关的成矿事件,代表的铁硫化物的成矿年龄为112.6～126 Ma.     

对流云街激发的重力波和波动阻力

大气边界层中的对流活动,可以在其上部稳定层中激发出重力波,并引起垂直动量输送,影响到对流层和平流层中的动量平衡过程.从二层模式中大气波动方程的线性解出发,得出了对流云街激发的重力波波阻解析表达式,并讨论了大气条件对波阻的影响.这些分析可有助于大气环流模式(GCM)中此类重力波波阻参数化表达式的建立和改进.     

Carbon Isotope Geochemistry of the Orinoco Basin

Stable carbon isotope ratios have been used to study the sources of particulate organic carbon (POC) and total dissolved inorganic carbon in the Orinoco Basin. The isotopic composition of total dissolved inorganic carbon shows a range of from -8·1 to -23·0 ppt, an indication of dominance of biological processes. The isotopic composition of POC exhibits a range of from -24·1 to -34·6 ppt with little seasonal variation. The isotopic evidence indicates that the POC is predominantly of terrestrial origin rather than a result of in situ planktonic production. The similarity of isotopic composition of POC and coastal sediments suggests that riverine organic detritus has been transported 30-50 km offshore in a direction parallel to the Orinoco river channel.     

Genetic Programming Approach for Predicting Surface Subsidence Induced by Mining

INTRODUCTIONThe study of surface subsidence resulting fromunderground mining holds significance in view ofbeneficial effects on national economy and people’sliving conditions.As is known,a large quantity ofminerals need to be exploited for modernization con-struction.Mining leads to surface subsidence,someunderground engineering and production facilities aredestroyed accordingly,which blocks the developmentof production and retards people’s living standard.To solve this contradiction,su…     

孤立金属尖端的电晕触发阈值

为了得到金属尖端在发生电晕放电时尖端处的电场强度,该文首先采用实验室实验得到不同高度、不同形状、不同材质的金属尖端发生电晕放电时的环境电场阈值;再采用有限元法计算二维泊松方程,得到尖端处电晕触发阈值,由此得出以下结论:环境电场阈值随金属尖端高度的增大基本呈线性减小趋势,随着尖端越来越尖,环境电场阈值呈先减小后增大的变化趋势;高度、形状对金属物尖端处电晕触发阈值无影响,尖端处电晕阈值为定值;给出尖端处电晕触发阈值为158.75 kV·m-1与空间分辨率的拟合公式,可为今后电晕放电数值模拟中判断电晕放电的起始时刻提供参考。     

用超长基线解算分析汶川地震动态形变特征

以远距离的IGS武汉GPS站为参考站,用超长基线双差瞬时精密定位技术,获取汶川Ms8.0地震震时震区连续GPS站1 Hz动态形变序列。同时以较近距离的雅安站为参考站解算动态形变序列,结果显示,两者的形变结果具有很好的一致性。距离断层较近的郫县、成都、绵阳、中江等站形变较大（形变最大的郫县站最大振幅达1.034 m）;位于震中南侧的邛崃、雅安等站形变较小。利用动态时序的地震波到时和震中距离估计地震波地壳平均速度为3.1 km/s。     

Molecular cloning of the heat shock protein 20 gene from Paphia textile and its expression in response to heat shock

P. textile is an important aquaculture species in China and is mainly distributed in Fujian, Guangdong, and Guangxi Provinces. In this study, an HSP20 c DNA designated Pt HSP20 was cloned from P. textile. The full-length c DNA of Pt HSP20 is 1 090 bp long and contains a 5′ untranslated region(UTR) of 93 bp, a 3′ UTR of 475 bp, and an open reading frame(ORF) of 522 bp. The Pt HSP20 c DNA encodes 173 amino acid residues and has a molecular mass of 20.22 k Da and an isoelectric point of 6.2. Its predicted amino acid sequence shows that Pt HSP20 contains a typical α-crystallin domain(residues 77–171) and three polyadenylation signal-sequences at the C-terminus. According to an amino acid sequence alignment, Pt HSP20 shows moderate homology to other mollusk s HSPs. Pt HSP20 m RNA was present in all of the test tissues including the heart, digestive gland, adductor muscle, gonad, gill, and mantle, with the highest concentration found in the gonad. Under the stress of high temperature, the expression of Pt HSP20 m RNA was down-regulated in all of the tissues except the adductor muscle and gonad.     

Antioxidative capacity and enzyme activity in Haematococcus pluvialis cells exposed to superoxide free radicals

The antioxidative capacity of astaxanthin and enzyme activity of reactive oxygen eliminating enzymes such as superoxide dismutase (SOD), peroxidase (POD), catalase (CAT) and ascorbate peroxidase (APX) were studied in three cell types of Haematococcus pluvialis exposed to high concentrations of a superoxide anion radical (O₂⁻). The results show that defensive enzymes and astaxanthin-related mechanisms were both active in H. pluvialis during exposure to reactive oxygen species (ROS) such as O₂⁻. Astaxanthin reacted with ROS much faster than did the protective enzymes, and had the strongest antioxidative capacity to protect against lipid peroxidation. The defensive mechanisms varied significantly between the three cell types and were related to the level of astaxanthin that had accumulated in those cells. Astaxanthin-enriched red cells had the strongest antioxidative capacity, followed by brown cells, and astaxanthin-deficient green cells. Although there was no significant increase in expression of protective enzymes, the malondialdehyde (MDA) content in red cells was sustained at a low level because of the antioxidative effect of astaxanthin, which quenched O₂⁻ before the protective enzymes could act. In green cells, astaxanthin is very low or absent; therefore, scavenging of ROS is inevitably reliant on antioxidative enzymes. Accordingly, in green cells, these enzymes play the leading role in scavenging ROS, and the expression of these enzymes is rapidly increased to reduce excessive ROS. However, because ROS were constantly increased in this study, the enhance enzyme activity in the green cells was not able to repair the ROS damage, leading to elevated MDA content. Of the four defensive enzymes measured in astaxanthin-deficient green cells, SOD eliminates O₂⁻, POD eliminates H₂O₂, which is a by-product of SOD activity, and APX and CAT are then initiated to scavenge excessive ROS.