A comparison of sea-state parameters from nautical radar images and buoy data

Nautical radar and scalar buoy measurements of ocean wind generated waves have been analysed to compare the spectral parameters estimated from both sensors. The time series of different sea-state parameters and the differences and ratios of the values obtained from radar and buoy data using different analysis methods are compared. It has been observed that main differences between the sea-state parameters derived by using measurements obtained from both sensors result both from device characteristics and from the method of spectral estimation. In particular, it is shown that the Nyquist frequency has an important effect on the value of the sea-state parameters depending on spectral moments of order higher than zero.     

Analysis of directional wave fields using X-band navigation radar

The aim of this work is to present an approach to describe complex sea states including the ones consisting of superpositions of swell and wind sea components, using a nautical radar in X-band as a remote sensing technique. In the present article, the inversion method to obtain the spectral representation of the wave fields is described. The method is applied to analyse data obtained from simulation techniques, as well as from measurements obtained during oceanographic campaigns in the Bay of Biscay and North Sea.     

Wave hindcasting off the coast of Portugal

A study was conducted applying a second-generation wave model to predictions in coastal zones. The model was calibrated with wave measurements conducted off the Portuguese coast, for a period of 6 months. The wind fields used in the calculation were supplied by the European Centre for Medium Range Weather Forecast (ECMRWF). The calibrated model allows good predictions of significant wave height. Satisfactory comparisons have been made with predictions of the WAM model     

Ras gene in marine mussels: a molecular level response to petrochemical exposure

Mussels are susceptible to numerous toxicants and are often employed as bioindicators. This study investigated the status of the ras proto-oncogene in Mytilus galloprovincialis following petrochemical exposure. A M. galloprovincialis homologue of the vertebrate ras gene was isolated, showing conserved sequence in regions of functional importance and a high incidence of polymorphic variation. Mutational damage was investigated in mussels chronically exposed to the water-accommodated fraction of #4 fuel-oil (WAF), and in mussels collected along the NW coast of Portugal in sites with different levels of petrochemical contamination. A ras gene point mutation was identified in the codon 35 of one individual exposed to 12.5% WAF. No mutations were detected in mussels from the WAF control or environmental samples. This represents the first report of a ras gene mutation, experimentally-induced by petrochemical exposure, in an invertebrate species.     

Microbiological monitoring of bivalves from the Ria Formosa Lagoon (south coast of Portugal): a 20 years of sanitary survey

The microbiological pollution of coastal waters is a major problem, especially in shellfish areas. This article shows the faecal contamination in bivalves from the Ria Formosa Lagoon (south coast of Portugal) along 20 years (1990-2009). The highest values of Escherichia coli in bivalves were obtained during the 90s, related with the discharge of untreated wastewaters and agricultural runoff. In the 2000s contamination levels decreased, with 83% of the population already served by new or remodelled sewage treatment plants. The highest levels were found in bivalves close to the largest city, where punctual and diffuse contamination sources still exist. Bivalves from the less impacted site showed the lowest contamination, an area with more water renewal. Seasonally, the highest levels were in autumn and winter, due to the runoff of waters from rainfall. These were opposite to those in spring and summer, when the highest temperatures and salinity showed a bactericidal effect.     

The use of cholinesterase as potential biomarker: In vitro characterization in the polychaete Capitella teleta

The ecological relevance of polychaetes coupled with their easy culture and maintenance in the laboratory, has led them to become increasingly used in marine ecotoxicological studies, raising the need to validate frequently applied monitoring tools at various biological levels. The present study was aimed to characterize the cholinesterases (ChE) activity in the polychaete Capitella teleta, using three substrates (acetylthiocholine iodide, propionylthiocholine iodide, and S-butyrylthiocholine iodide) and four known inhibitors (eserine hemisulfate, BW284c51, iso-OMPA and chlorpyrifos-oxon). Results showed that most of the measured cholinesterase activity was acetylcholinesterase (AChE). Inhibition of enzyme kinetic experiments denoted that sensitivity of C. teleta’s ChE to the organophosphorous metabolite chlorpyrifos-oxon (IC₅₀ = 60.72 nM) was analogous to some fish species. This study highlights the relevance of ChE characterization before its use as a biomarker in ecotoxicology and biomonitoring studies.     

Associations between outdoor air quality and birth weight: a geostatistical sequential simulation approach in Coastal Alentejo,Portugal

Several environmental health studies suggest birth weight is associated with outdoor air pollution during gestation. In these studies, exposure assignments are usually based on measurements collected at air quality monitoring stations that do not coincide with health data locations. So, estimated exposures can be misleading if they do not take into account the uncertainty of exposure estimates. In this article we conducted a semi-ecological study to analyze associations between air quality during gestation and birth weight. Air quality during gestation was measured using a biomonitor, as an alternative to traditional air quality monitoring stations data, in order to increase spatial resolution of exposure measurements. To our knowledge this is the first time that the association between air quality and birth weight is studied using biomonitors. To address exposure uncertainty at health locations, we applied geostatistical simulation on biomonitoring data that provided multiple equally probable realizations of biomonitoring data, with reproduction of observed histogram and spatial covariance while matching for conditioning data. Each simulation represented a measure of exposure at each location. The set of simulations provided a measure of exposure uncertainty at each location. To incorporate uncertainty in our analysis we used generalized linear models, fitted simulation outputs and health data on birth weights and assessed statistical significance of exposure parameter using non-parametric bootstrap techniques. We found a positive association between air quality and birth weight. However, this association was not statistically significant. We also found a modest but significant association between air quality and birth weight among babies exposed to gestational tobacco smoke.