底栖有孔虫(Florilus decors)壳体微量元素/钙比值的LA-ICP-MS原位微区分析方法及应用——以长江口外海域为例

深海沉积物中有孔虫壳体的微量元素、同位素测试技术已较为成熟,而河口近海沉积物中有孔虫壳体元素微区测试却鲜有报道。本文报道了一种利用激光剥蚀电感耦合等离子体质谱（LA-ICP-MS）分析法测试取自长江口外沉积物中的有孔虫壳体元素/钙比值的技术,研究了氦气载气流量、能量密度、束斑大小、激光剥蚀频率等参数对测试结果的影响,优化了活体有孔虫原位分析方法。应用确定的测试条件对2016年7月取自长江口外的底栖有孔虫优美花朵虫样品（Florilus decors）进行测试,发现Mg、Sr等元素/钙比值在壳体不同位置上无显著差异,而Mn的数据较为离散,可能与有孔虫生长过程中经历的水环境及其变化有关。     

利用扩增vvhA基因的环介导等温扩增技术快速，特异而敏感地检测创伤弧菌

创伤弧菌是一种可感染人类的河口病原菌。建立快速,特异而敏感的检测方法,有助于创伤弧菌感染的早期疾病诊断和及时治疗。本研究设计了针对vvhA基因的一系列引物（包括两对外部引物和两对内部引物）,采用环介导等温扩增技术（LAMP）来检测创伤弧菌。结果显示,本方法的最适扩增温度是63℃,反应仅需35分钟。扩增产物不仅可以用含有DNA ladder的琼脂糖凝胶电泳检出,也可借助钙黄绿素直接肉眼观察。采用45株菌株检测该方法的特异性,其中所有创伤弧菌均被检出而其他菌株检测结果皆为阴性。本方法的敏感性是普通PCR扩增的100倍,同时利用该方法可以准确检测出所有的模拟样品、临床标本及环境样品。与其他已知方法比较,针对vvhA基因的介导等温扩增技术可以快速、简单、敏感及特异地鉴定创伤弧菌。     

超慢速扩张环境下超镁铁质岩系统的热液硫化物成矿机理以及启示

西南印度洋63.5°E热液区是在超慢速扩张洋脊发现的首个超镁铁质岩热液系统。对取自该区的热液硫化物样品进行了系统的矿物学和地球化学分析,矿物学分析结果表明:该热液区硫化物为富Fe型高温硫化物,且经历了较深程度的氧化蚀变,大量中间态的Fe氧化物充填在硫化物矿物间的孔隙及内部解理中;这些硫化物相以白铁矿为主,其次是等轴古巴矿和少量铜蓝,缺乏黄铁矿、闪锌矿。据推断,该区的热液成矿作用分为4个阶段:低温白铁矿阶段→高温等轴古巴矿阶段→自形白铁矿阶段→后期海底风化阶段（少量铜蓝以及大量的Fe的羟氧化物）。与之相对应,地球化学分析结果表明这些硫化物的Fe含量较高（31.57%~44.59%）,Cu含量次之（0.16%~7.24%）,而Zn含量普遍较低（0.01%~0.11%）;微量元素较为富集Co（328×10-6~2 400×10-6）和Mn（48.5×10-6~1 730×10-6）。该区硫化物中较高含量的Fe、Co与超镁铁质岩热液系统相似,明显高于镁铁质岩热液系统。独特的热液硫化物矿物学特征和元素组成可能与该区普遍出露的地幔岩、橄榄岩蛇纹石化作用以及拆离断层的广泛发育的环境有关。     

强光照和黑暗条件下刺参体壁的基因表达差异

参对光照变化非常敏感,研究刺参对光照的分子响应非常重要。本研究应用RNA测序获取了刺参暴露于强光（“强光”）、正常光照（“对照”）和完全黑暗（“黑暗”）环境下体壁的基因表达谱情况,通过“对照”与“黑暗”,“对照”与“强光”和“黑暗”与“强光”的比较,在|log₂ ratio|≥1和FDR≤0.001的标准下分别发现了1161、113和1705个差异表达基因（DEGs）。基因本体分析表明,“cellular process”和“binding”在“生物过程”和“分子功能”类别中的DEGs富集最多,而“cell”和“cell part”在“细胞组分”类别中的DEGs富集最多。将DEGs与Kyoto Encyclopedia基因和基因组数据库上的于214、41和229条通路进行比对,发现了51、2和57条通路分别显著富集。本研究发现的光特异性DEGs可作为深入研究刺参对光照变化的生化适应机制的重要目标基因。     

Numerical Simulation of the Vortex-Induced Vibration of A Curved Flexible Riser in Shear Flow

A series of fully three-dimensional(3 D) numerical simulations of flow past a free-to-oscillate curved flexible riser in shear flow were conducted at Reynolds number of 185–1015. The numerical results obtained by the two-way fluid–structure interaction(FSI) simulations are in good agreement with the experimental results reported in the earlier study. It is further found that the frequency transition is out of phase not only in the inline(IL) and crossflow(CF) directions but also along the span direction. The mode competition leads to the non-zero nodes of the rootmean-square(RMS) amplitude and the relatively chaotic trajectories. The fluid–structure interaction is to some extent reflected by the transverse velocity of the ambient fluid, which reaches the maximum value when the riser reaches the equilibrium position. Moreover, the local maximum transverse velocities occur at the peak CF amplitudes, and the values are relatively large when the vibration is in the resonance regions. The 3 D vortex columns are shed nearly parallel to the axis of the curved flexible riser. As the local Reynolds number increases from 0 at the bottom of the riser to the maximum value at the top, the wake undergoes a transition from a two-dimensional structure to a 3 D one. More irregular small-scale vortices appeared at the wake region of the riser, undergoing large amplitude responses.     

Least Square Finite Element Method for Viscous Splitting of Unsteady Incompressible Navier–Stokes Equations

In order to solve unsteady incompressible Navier–Stokes(N–S) equations, a new stabilized finite element method,called the viscous-splitting least square FEM, is proposed. In the model, the N–S equations are split into diffusive and convective parts in each time step. The diffusive part is discretized by the backward difference method in time and discretized by the standard Galerkin method in space. The convective part is a first-order nonlinear equation.After the linearization of the nonlinear part by Newton's method, the convective part is also discretized by the backward difference method in time and discretized by least square scheme in space. C~0-type element can be used for interpolation of the velocity and pressure in the present model. Driven cavity flow and flow past a circular cylinder are conducted to validate the present model. Numerical results agree with previous numerical results, and the model has high accuracy and can be used to simulate problems with complex geometry.     

Walking Access Distance of Metro Passengers and Relationship with Demographic Characteristics: A Case Study of Nanjing Metro

In the metropolises of China, the metro plays an increasingly important role in commuting because of its efficiency, affordability, and cleanliness. This paper attempts to explore the relationship between walking access distance to metro stations and the demographic characteristics of passengers, such as age, monthly income, travel frequency, gender, and travel purpose, as well as the influence of the urban context. Nanjing Metro Line 2 is selected as the case study. By using different methods such as a questionnaire survey, spatial decay function, analysis of covariance (ANOVA), network analysis of routes, and K-means cluster analysis, it is suggested that demographic characteristics have a significant impact on the pedestrian walking distance, with the exception of gender. Furthermore, the paper finds a spatial decay effect in walking access distance, the decay rate of which, however, varies across stations. Terminal stations have a larger pedestrian catchment area than in regular and exchange stations. Moreover, the passengers of Nanjing Metro Line 2 can be classified into six groups according to their demographic characteristics, among which education and occupation are vital indicators in determining their willingness to walk to the stations. Middle-class passengers have a higher dependence on the metro and tend to walk longer than other groups do. This study provides an important reference for planners and transport sectors to optimize land-use and transport infrastructures.     

Optimization Transmission Theory and Technical Pathways that Describe Multiscale Urban Agglomeration Spaces

Urban agglomerations are spatial entities that promote the development of ‘new urbanization' processes within China. In this context, the concept of ‘multiscale urban agglomeration spaces' encompasses three linked levels: macroscale urban agglomerations, mesoscale cities, and microscale urban centers. Applying a series of multidisciplinary integrated research methods drawn from geography, urban planning, and architecture, this paper reveals two intensive utilization laws that can be generalized to apply to multiscale urban agglomeration spaces, top-to-bottom ‘positive transmission' linkage and inside-to-outside ‘negative transmission' movement. This paper also proposes optimization transmission theory and policy decision technical pathways that can be applied to these three urban agglomeration spatial scales. Specific technical pathways of transmission include intensive expansion and simulated decision-making in macroscale urban agglomerations, ecology, production, and living space intensive layout and dynamic decision-making in mesoscale cities, and four cores(i.e., ‘single, ring, axis, and pole core') progressive linkage and intensive optimization decision-making in microscale urban centers. The theory and technical pathways proposed in this paper solve the technical problem of optimization and provide intensive methods that can be applied not only at the individual level but also at multiple scales in urban agglomeration spaces. This study also advances a series of comprehensive technical solutions that can be applied to both compact and smart growth cities as well as to urban agglomerations. Solid theoretical support is provided for the optimization of Chinese land development, urbanization, agricultural development, and ecological security.     

Binary Star Fractions from the LAMOST DR4

Stellar systems composed of single, double, triple or higher-order systems are rightfully regarded as the fundamental building blocks of the Milky Way. Binary stars play an important role in formation and evolution of the Galaxy. Through comparing the radial velocity variations from multiepoch observations, we analyze the binary fraction of dwarf stars observed with LAMOST. Effects of different model assumptions, such as orbital period distributions on the estimate of binary fractions,are investigated. The results based on log-normal distribution of orbital periods reproduce the previous complete analyses better than the power-law distribution. We find that the binary fraction increases with Teff and decreases with [Fe/H]. We first investigate the relation between α-elements and binary fraction in such a large sample as provided by LAMOST. The old stars with high [α/Fe] dominate with a higher binary fraction than young stars with low [α/Fe]. At the same mass, earlier forming stars possess a higher binary fraction than newly forming ones, which may be related with evolution of the Galaxy.     

A model of fast radio bursts: collisions between episodic magnetic blobs

Fast radio bursts(FRBs) are bright radio pulses from the sky with millisecond durations and Jansky-level flux densities. Their origins are still largely uncertain. Here we suggest a new model for FRBs. We argue that the collision of a white dwarf with a black hole can generate a transient accretion disk, from which powerful episodic magnetic blobs will be launched. The collision between two consecutive magnetic blobs can result in a catastrophic magnetic reconnection, which releases a large amount of free magnetic energy and forms a forward shock. The shock propagates through the cold magnetized plasma within the blob in the collision region, radiating through the synchrotron maser mechanism,which is responsible for a non-repeating FRB signal. Our calculations show that the theoretical energetics, radiation frequency, duration timescale and event rate can be very consistent with the observational characteristics of FRBs.