大菱鲆卵黄原蛋白的分离纯化及Native-PAGE、SDS-PAGE性质鉴定

采用5 mg/kg 17β-雌二醇对大菱鲆(Scophthalmus maximus)进行肌肉注射,一周后尾静脉取血,离心分离获得血浆,经Sephacryl S-300(高分辨)分子筛纯化卵黄原蛋白,对卵黄原蛋白进行常规聚丙烯酰胺凝胶电泳,考马斯亮蓝染色和糖、磷、脂的特征性基团染色.结果表明,17β-雌二醇能诱导大菱鲆产生卵黄原蛋白,以N-ative-PAGE方法计算得到大菱鲆卵黄原蛋白的分子质量为538 ku,以SDS-PAGE方法得出其两种亚基的分子质量分别为100 ku和82 ku.分子筛凝胶过滤能较好地纯化大菱鲆血浆中的卵黄原蛋白.     

底栖生物生产力现场测试系统(BCSPM)的建立及其初步实验结果

为对生态系统的健康评估及底栖生物资源的开发利用提供技术支撑,在胶州湾筏式养殖水域,建立了底栖生物生产力现场测试系统(BCSPM),并在光照和黑暗条件下测定了沉积物海水界面溶解氧随时间的变化、计算了氧通量、估算了底栖总初级生产力和群落呼吸。在光照较强的中午时刻测定的群落总生产力为20．76mgCm^-2h^-1;养殖水域沉积物中的群落呼吸要高于邻近水域。BCSPM整体运转良好,初步结果为系统的改进和进一步实验提供了依据。     

基于标准化降水蒸散指数（SPEI）的毛乌素沙地1981—2020年干旱特征研究

大气干旱是影响半干旱沙区植被建设、生态恢复及社会经济可持续发展的重要因素。基于1981—2020年毛乌素沙地10个气象站点的逐月气象资料,计算了月、季和年尺度的标准化降水蒸散指数（SPEI）,分析了该沙区近40年降水和气温的变化趋势、干旱事件及其频率的时空特征。结果表明：（1）毛乌素沙地近40年降水量和气温均呈现显著的上升趋势（P<0.05）;秋季和冬季降水量呈现显著的上升趋势（P<0.05）,四季气温均呈现显著的上升趋势（P<0.05）。（2）毛乌素沙地总体上呈现出不显著的湿润化趋势（P>0.05）,但秋季呈显著的湿润化趋势（P<0.05）;中、西部地区呈现出湿润化趋势,而东部地区则呈现干旱化趋势。（3）近40年毛乌素沙地的月尺度下干旱总频率达32.71%,各等级发生频率为轻旱>中旱>重旱>特旱,季节发生频次为冬季>夏季、秋季>春季;轻旱主要发生在毛乌素沙地的北部、中部、东南和西南部,中旱在东部、北部和西部边缘,重旱在东部、中部以及南部地区,特旱在西北部、南部和东南部区域。     

新时期城市群理论内涵的再认知

在全球化、信息化背景下,城市之间的竞争已经不再局限于单体城市本身的规模属性,而是越来越取决于城市参与分工协作的地位以及多尺度空间的功能联系,尤其表现在城市网络化、集群化的竞合态势,而城市群已经成为该背景下具有全球意义的空间组合模式.在系统梳理了中西方城市功能地域概念的内在关联基础上,论文认为,中国的城市群概念具有尺度伸...     

Soil Organic Carbon Contents and Stocks in Coastal Salt Marshes with Spartina alterniflora Following an Invasion Chronosequence in the Yellow River Delta,China

Plant invasion alters the fundamental structure and function of native ecosystems by affecting the biogeochemical pools and fluxes of materials and energy. Native(Suaeda salsa) and invasive(Spartina alterniflora) salt marshes were selected to study the effects of Spartina alterniflora invasion on soil organic carbon(SOC) contents and stocks in the Yellow River Delta. Results showed that the SOC contents(g/kg) and stocks(kg/m~2) were significantly increased(P 0.05) after Spartina alterniflora invasion of seven years, especially for the surface soil layer(0–20 cm). The SOC contents exhibited an even distribution along the soil profiles in native salt marshes, while the SOC contents were gradually decreased with depth after Spartina alterniflora invasion of seven years. The natural ln response ratios(Ln RR) were applied to identify the effects of short-term Spartina alterniflora invasion on the SOC stocks. We also found that Spartina alterniflora invasion might cause soil organic carbon losses in a short-term phase(2–4 years in this study) due to the negative Ln RR values, especially for 20–60 cm depth. And the SOCD in surface layer(0–20 cm) do not increase linearly with the invasive age. Spearman correlation analysis revealed that silt + clay content was exponentially related with SOC in surface layer(Adjusted R~2 = 0.43, P 0.001), suggesting that soil texture could play a key role in SOC sequestration of coastal salt marshes.     

武汉主城区液化潜能分区研究

搜集并整理武汉主城区地震安全性评价控制性钻孔SPT-N资料,配合武汉市地震动参数小区划成果,采用规范法对各场地土层在罕遇地震作用下液化可能性进行判别。以液化指数作为分区指标并结合各场地坐标,绘制武汉主城区液化潜能分区图。分区图初步预测了罕遇地震发生以后,可能发生液化的区域及其严重性,且表明无论是分布面积还是液化潜能,长江右岸均大于长江左岸。通过与4次历史大震液化资料的对比分析,武汉主城区可能液化场地具有地下水位埋深浅、液化土层分布范围广、埋藏深度大的特点。     

中国省域煤矿事故时空分异特征及影响因素研究

煤矿事故给国家和人民带来巨大的生命财产损失,科学把握煤矿事故发生的宏观规律及区域煤矿安全的影响因素有助于促进煤矿安全生产。本文尝试采用GIS空间分析方法开展区域煤矿事故的研究,基于空间自相关性、时空演化规律方法研究了2006-2015年中国省域煤矿事故的时空分异特点,并分析了省域煤矿安全的影响因素及相对风险度。主要结论：①中国各省区之间煤矿事故存在明显的空间自相关性,但随着各省区煤矿安全生产形势改善情况的不同,其自相关性在不断下降;②以空间重心转移曲线分析中国煤矿事故发生的时空演化规律,10年间煤矿事故空间重心向东北方向转移。③重特大事故频发的省区由于监管措施的有力执行往往具有较低的煤矿百万吨死亡率;平均煤矿生产能力低的省区具有较高的煤矿百万吨死亡率;④建立基于解释变量的贝叶斯空间模型,以煤炭百万吨死亡率为指标评估中国各省区煤矿事故相对风险度,中国煤矿安全生产相对危险度存在明显的空间分布规律且各省区差异较大,相对危险度较高的省区主要集中在南方地区,包括湖北、福建等,危险度较低的省区主要集中在中东部地区,包括山西、内蒙古等。     

The higher grain production,the more social deprivation? A case study of Henan province in traditional agricultural areas of China

This paper examines the spatial-temporal dynamics of inequality from the perspective of social deprivation in the context of continuous growth of grain production using national census data from 2000 and 2010 for Henan province, China. Our analytical results show an overall level of social deprivation that is high and a widening social deprivation gap at the county level. The social deprivation shows great spatiotemporal heterogeneity, the higher deprived counties are mainly distributed in central and eastern Henan province, while counties with lower social deprivation are clustered in northwestern regions, formed a belt-shape spatial differential between the south (higher deprived counties clustered) and northwest (low deprived counties clustered) Henan in 2000, and changed toward a core-periphery semi annular spatial structure in 2010, the low deprived counties surrounded by high deprived counties. The social deprivation shows a “west low and east high” spatial pattern, similar to the grain production in Henan province. Socioeconomic development level was low in most counties in Yudong plain despite of keeping the continuous increase of grain production, meaning the higher grain output did not result in the more social development expected in Chinese traditional agricultural areas, and the regional disparity is expected to increase in the near future if development conditions are not changed, which could inspire more targeted rural governance options.     

Low-energy Lunar Trajectories with Lunar Flybystwo

The low-energy lunar trajectories with lunar flybys are investigated based on the Sun-Earth-Moon bicircular problem (BCP). The characteristics of the distribution of trajectories in the phase space are summarized. Using the invariant manifolds in the BCP system, the low-energy lunar trajectories with lunar flybys are sought. Then, take time as an augmented dimension in the phase space of a nonautonomous system, we present the state space map and reveal the distribution of these lunar trajectories in the phase space. Consequently, we find that the low-energy lunar trajectories exist as families, and that the every moment in the Sun-Earth-Moon synodic period can be the departure date. Finally, we analyse the velocity increment, transfer duration, and system energy for the different trajectory families, and obtain the velocity-impulse optimal family and the transfer-duration optimal family, respectively.     

Scientific prospects for spectroscopy of the gamma-ray burst prompt emission with SVOM

SVOM (Space-based multi-band astronomical Variable Objects Monitor) is a Sino-French space mission dedicated to the study of Gamma-Ray Bursts (GRBs) in the next decade, capable to detect and localise the GRB emission, and to follow its evolution in the high-energy and X-ray domains, and in the visible and NIR bands. The satellite carries two wide-field high-energy instruments: a coded-mask gamma-ray imager (ECLAIRs; 4–150 keV), and a gamma-ray spectrometer (GRM; 15–5500 keV) that, together, will characterise the GRB prompt emission spectrum over a wide energy range. In this paper we describe the performances of the ECLAIRs and GRM system with different populations of GRBs from existing catalogues, from the classical ones to those with a possible thermal component superimposed to their non-thermal emission. The combination of ECLAIRs and the GRM will provide new insights also on other GRB properties, as for example the spectral characterisation of the subclass of short GRBs showing an extended emission after the initial spike.