苯并（a）芘诱导下岩虫CYP基因的表达特征

Rapid amplification of cDNA ends(RACE) and real-time polymerase chain reaction(RT-PCR) were carried out to analyze the CYP4 gene expression in polychaete Marphysa sanguinea exposed to benzo[a]pyrene(BaP) in this study. The full length of MsCYP4 cDNA was 2 470 bp, and it encoded 512 amino acids. The deduced amino acid sequence showed 47% identity with CYP4 F from frog Xenopus tropicalis and shared high homology with other known CYP4 sequences. To analyse the role of CYP4 in protecting M. sanguinea from BaP exposure, three BaP groups were established: 0.5, 5 and 50 μg/L. Polychaetes were sampled after 3, 7 and 12 d. At 0.5 μg/L, the effect of BaP on MsCYP4 gene expression increased with time prolonged. MsCYP4 gene expression curve showed Ushaped trend with time in 5 and 50 μg/L BaP groups. Therefore, MsCYP4 gene may play an important role in maintaining the balance of cellular metabolism and protecting M. sanguinea from BaP toxicity.     

我国瓯江口和珠江口凤鲚洄游模式和生境利用研究

Habitat use of the tapertail anchovy(Coilia mystus Linnaeus, 1758) from the Oujiang River Estuary and the Zhujiang(Pearl) River Estuary was studied by examining the environmental signatures of Sr and Ca in otoliths using electron probe microanalysis. Individuals from the Oujiang River had higher and varied Sr:Ca ratios(expressed as(Sr:Ca)×1 000, 3.83–13.0 average) in the otolith core regions, suggesting that they were born in brackish or sea waters, and that a freshwater habitat might not be necessary for egg hatching and larval growth.While, individuals from the Zhujiang River had lower Sr:Ca ratios(0.39–2.51 average) in the core regions,suggesting a freshwater origin. After hatching, anchovies from the Zhujiang River migrate downstream to the river estuary close to brackish water. Our results demonstrated varied habitat use for spawning during stages of early life history between the two populations, and suggested that such variations are promoting diversity of life history strategies of this species.     

南海沉积物细菌胞外蛋白酶在家族水平上的多样性

海洋产蛋白酶细菌及其分泌的胞外蛋白酶在降解有机氮以推动海洋氮循环进行方面发挥着重要作用,但目前对这二者多样性的认识非常有限。本研究自南海沉积物中筛选得到90株产蛋白酶细菌,并通过N-端氨基酸序列,分析了其胞外蛋白酶在家族水平上的多样性。16S rRNA基因序列分析的结果表明,筛选的产蛋白酶细菌均属于Gammaproteobacteria纲,且大多数属于Alteromonadales目和Vibrionales目的不同属。对其中14株菌株的14个胞外蛋白酶的N-端氨基酸序列分析表明,所有这些蛋白酶属于金属蛋白酶的M4家族或丝氨酸蛋白酶的S8家族。本研究提供了海洋沉积物产蛋白酶细菌在类群及其胞外蛋白酶在类型上的新细节,这将有助于全面了解微生物酶促降解海洋沉积物有机氮的过程和机理。     

Vortex-Induced Vibrations of A Long Flexible Cylinder in Linear and Exponential Shear Flows

A numerical study based on a wake oscillator model was conducted to determine the response performance of vortex-induced vibration(VIV) on a long flexible cylinder with pinned-pinned boundary conditions subjected to linear and exponential shear flows. The coupling equations of a structural vibration model and wake oscillator model were solved using a standard central finite difference method of the second order. The VIV response characteristics including the structural displacement, structural frequency, structural wavenumber, standing wave behavior,travelling wave behavior, structural velocity, lift force coefficient and transferred energy from the fluid to the structure with different flow profiles were compared. The numerical results show that the VIV displacement is a combination of standing waves and travelling waves. For linear shear flow, standing waves and travelling waves dominate the VIV response within the low-velocity and high-velocity zones, respectively. The negative values of the transferred energy only occur within the low-velocity zone. However, for exponential shear flow, travelling waves dominate the VIV response and the negative energy occurs along the entire length of the cylinder.     

西太平洋马里亚纳海沟附近海山的金相柳珊瑚属(珊瑚虫纲:软珊瑚目:金柳珊瑚科)二新记录种

为探究热带西太平洋海山生物多样性,2016年利用"发现"号遥控无人潜水器(ROV)对西太平洋马里亚纳海沟附近的M2海山进行了采样调查,其中获得了3个深水金相柳珊瑚样本。通过光学显微镜和扫描电镜观察,鉴定其为黑发金相柳珊瑚Metallogorgia melanotrichos(WrightStuder,1889)和长刺金相柳珊瑚Metallogorgia macrospina Kükenthal,1919。这两种金相柳珊瑚均为该区域的新记录种,对其作了详细的形态描述,并依据特征性状对该属所有物种作了分类检索。     

长江三角洲蓝图重绘的基础科学问题:进展与未来研究

在全球气候变化和人类活动影响加剧的背景下,作为河口海岸重要子系统的三角洲正在发生快速变化。长江三角洲地处长江入东海交汇处,是中国最重要的经济核心区之一,对邻近区域乃至整个长江经济带经济社会发展都起着重要作用。由于全球变暖、海面上升和强烈人类活动引发了三角洲系统状态转换,因此以往基于恒定系统状态而获得的有关长江三角洲的认识已不能满足未来需求,迫切需要对未来海面变化、极端事件、流域与河口工程影响下的三角洲物质循环条件、物理过程、地貌冲淤演化、源-汇格局调整等科学问题进行深入研究。在三角洲系统行为、未来演化趋势的预测能力建设中,应重视从海面到海底的综合立体观测系统的发展,以获取关键数据;基于三角洲系统的时、空演化特征,建立三角洲本征态和衍生态的谱系理论。未来需针对系统状态转换而调整原先的经济社会发展模式,以便保护自然资源、重建生态系统,更好地支撑长江经济带发展,重绘长江三角洲发展蓝图。     

京津唐地区HFMD时空变异分析与影响因子探测

手足口病（HFMD）是一种多发于儿童的常见传染病。近年来,中国HFMD发病人数逐年上升,疾病疫情也日益受到社会广泛关注。虽已有不少相关研究,但对于探测其时空异质性及量化潜在影响因子解释力的研究仍然较少。本文采用地理探测器及贝叶斯时空层次模型,对2009-2013年京津唐地区HFMD发病率的时空异质性进行分析,并量化各影响因子及其两两交互作用对HFMD发病率的解释力。结果表明：① HFMD的相对风险存在时间异质性,其发病风险在春夏季（5-7月）达到峰值,而冬季（12-次年2月）发病风险最低;② HFMD的相对风险存在空间异质性,在经济发达的地区HFMD发病率较高;③ 影响HFMD发病率时间异质性的主要气象因子为平均温度、累积降水、相对湿度,解释力分别为0.38,0.27,0.13,且交互作用都大于独自影响的作用,如平均温度和相对湿度、平均温度和降水,平均温度和风速两两交互的解释力分别为0.43, 0.40, 0.42。通过研究京津唐地区HFMD发病率的时空异质性以及影响因子的量化状况,为本地区HFMD的预防和控制提供理论依据。     

Formation and evolution of the tertiary carbonate reefs in the Madura Strait Basin of Indonesia

Analysis of 2D seismic data over 4 500 km in length from the Madura Strait Basin in the East Java Sea reveals seismic reflection characteristics of reefs and associated sedimentary bodies, including asymmetrical or symmetrical dome reflections, slope progradational reflections, chaotic reflections and discontinuous strong reflections inside the reef, which onlap the flank of the reef. It is concluded that the developmental paleo-environment of most reefs is mainly conducive to shallow marine carbonate platform facies and platform margin facies, based on well core data, variations in seismic facies and strata thickness. The formation and evolution of all reefs are primarily influenced by the tectonic framework of the Madura Strait Basin. Platform margin reefs are principally controlled by two types of structures: one is a series of E-W trending Paleogene normal faults, and the other is an E-W trending Neogene inversion structures. In addition, wave actions, tidal currents and other ocean currents play an accelerated role in sorting, rounding and redeposition for the accumulation and evolution of reefs. Tertiary reefs in the MSB can be divided into four types: 1) an open platform coral reef of Late Oligocene to Early Miocene, 2) a platform margin coral reef controlled by normal faults in Late Oligocene to Early Miocene, 3) a platform margin Globigerina moundreef controlled by a “hidden” inversion structure in Early Pliocene, and 4) a platform margin Globigerina mound-reef controlled by thrust faults in the early Pliocene. Patterns of the formation and evolution of reefs are also suggested.

     

Karyological analysis of the sea cicada Blepharipoda liberate Shen from the Rizhao intertidal zone,China

Journal of Oceanology and Limnology - Blepharipoda liberate Shen is a commercially valuable seafood species that has important ecological significance in Shandong Province, China. Although B....     

Isolation of protein from Chlorella sorokiniana CY1 using liquid biphasic flotation assisted with sonication through sugaring-out effect

Microalgae, a sustainable source of multi beneficial components has been discovered and could be utilised in pharmaceutical, bioenergy and food applications. This study aims to investigate the sugaring-out effect on the recovery of protein from wet green microalga, Chlorella sorokiniana CY1 which was assisted with sonication. A comparison of monosaccharides and disaccharides as one of the phaseforming constituents shows that the monosaccharides, glucose was the most suitable sugar in forming the phases with acetonitrile to enhance the production of protein(52% of protein). The protein productivity of microalgae was found to be significantly influenced by the volume ratio of both phases, as the yield of protein increased to 77%. The interval time between the sonication as well as the sonication modes were influencing the protein productivity as well. The optimum protein productivity was obtained with 10 s of resting time in between sonication. Pulse mode of sonication was suitable to break down the cell wall of microalgae compared to continuous mode as a lower protein yield was obtained with the application of continuous mode. The optimum condition for protein extraction were found as followed: 200 g/L glucose as bottom phase with volume ratio of I:1.25, 10 s of resting time for ultrasonication, 5 s of ultrasonication in pulse mode and 0.25 g of biomass weight. The high yield of protein about 81% could be obtained from microalgae which demonstrates the potential of this source and expected to play an important role in the future.