Chlorinated hydrocarbons in seawater: Analytical method and levels in the northeastern Pacific

A method is described for analyzing nanogram quantities of chlorinated hydrocarbons from 1-1 samples of seawater. Seawater samples are pumped through a copper column containing a mixture by weight of 5% activated carbon powder, 10% MgO and 85% refined diatomaceous earth. The chlorinated hydrocarbons in the seawater are adsorbed or trapped on the column and subsequently eluted with 30% benzene in acetone (v/v) for analysis by gas-liquid chromatography.This procedure was used to analyze chlorinated hydrocarbon levels in samples collected off the southern California coast. We suggest that anthropogenic chlorinated hydrocarbons can be used for the investigation of large-scale ocean currents and mixing processes.     

Histopathological biomarkers in estuarine fish species for the assessment of biological effects of contaminants

The increasing emphasis on the assessment and monitoring of estuarine ecosystems has highlighted the need to deploy appropriate biological indices for these locations. Fish diseases and histopathology, with a broad range of causes, are increasingly being used as indicators of environmental stress since they provide a definite biological end-point of historical exposure. This study reports on the histopathological alterations observed in selected organs and tissues of three species of estuarine fish (Platichthys flesus, Pomatoschistus minutus and Zoarces viviparus), captured from four British estuaries (the Tyne, Tees, Mersey and Alde), differently impacted by contaminants, including PAHs. A biannual sampling regime was used to identify the important seasonal variations that occur in terms of the observed biological effects. Inflammatory lesions and hepatocellular fibrillar inclusions attained their highest prevalence in P. flesus captured from the Tyne, Tees and Mersey. The presence of pre-neoplastic and neoplastic toxicopathic lesions was highest in P. flesus captured from these sites, when compared to fish from the Aide reference site. In particular, the prevalence of hepatic foci of cellular alteration (up to 43.3%) and hepatocellular adenoma (up to 10%) were highest in P. flesus captured from the Mersey estuary. Intersex (ovotestis) was only recorded in male P.flesus captured from the Mersey estuary (up to 8.3%) and from male Z. viviparous captured from the Tyne estuary (25%). Pathologies associated with the gill and the kidney were also most prevalent in fish captured from the Tyne, Tees and Mersey estuaries. This study has successfully applied histopathology to an estuarine monitoring program, both for the recording of toxicopathic lesions in the liver and other organs, and for the detection of the endpoint of endocrine disruption, intersex. As such, it provides a powerful integrative tool for the assessment of biological effects of contaminants in these environments.     

Can turbidity caused by Corophium volutator (Pallas) activity be used to assess sediment toxicity rapidly?

The standard toxicity test organism, Corophium volutator, exhibits a behavioural response to contaminated sediments that causes increased turbidity of overlying water. We quantify the effects of this response to an estuarine sediment spiked with copper and hydrocarbon contaminated sediments from an oil installation in the North Sea. Turbidity measured 24 h after the start of a toxicity test shows a strong relationship with contaminant concentrations and with mortality after 10 days. Turbidity measurements can therefore give a rapid indication of sediment toxicity, permitting a reduction in storage time of sediments to be used in dilution series and toxicity identification evaluation (TIE) tests, reducing the likelihood of contaminants degrading prior to testing.     

Seasonality and selectivity in the feeding ecology and reproductive biology of deep-sea bathyal holothurians

Chlorophyll and carotenoid pigments were determined from the gut sediments of five species of bathyal holothurian in the NE Atlantic, sampled shortly after the spring/summer phytoplankton bloom in 2001 and prior to the spring bloom in 2002. Three species, Laetmogone violacea, Paroriza pallens and Bathyplotes natans, sampled within a similar depth range (900–1100 m) in the summer of 2001 showed significant differences in their chlorophyll and carotenoid pigment concentrations. This suggests they may select for slightly different components from the available food resource. Four species sampled in early spring 2002, Laetmogone violacea, Paroriza pallens, Benthogone rosea and Benthothuria funebris, also had significant differences in their pigment concentrations. These species were sampled over a wider depth range (1000–3100 m) showing a bathymetric trend in pigment concentrations. There was a distinct seasonal change in the composition and concentration of the pigments, linked to a reduction in the availability of fresh organic material during autumn and winter periods.Ovarian tissue was also examined. The carotenoid pigments found in the ovary also occurred in the OM ingested by the holothurians. The dominant gonadal carotenoid pigments were β-carotene, echinenone and zeaxanthin. The potential for using these carotenoids to gain a competitive advantage through selectivity of chlorophyll and carotenoid pigment biomarkers are discussed in relation to competition for food resources by deposit-feeders. The results were also compared with selectivity in abyssal species.     

Stratigraphy of the sediment infill in Bosphorus Strait: water exchange between the Black and Mediterranean Seas during the last glacial Holocene

The sediment infill over the Paleozoic bedrock in the Bosphorus Strait consists of four sedimentary units which were deposited in the last 26,000 ¹⁴C years B.P. The stratigraphy of these units suggests that this part of the Bosphorus was a freshwater lake between 26,000 and 5,300 ¹⁴C years B.P., depositing sands with a freshwater mollusc fauna of Black Sea neo-euxinian affinity (Dreissena rostriformis, Dreissena polymorpha, and Monodacna pontica). The first appearance of euryhaline Mediterranean molluscs (e.g., Ostrea edulis, Mytilus edulis) was observed at 5,300 ¹⁴C years B.P. in this part of the Bosphorus. Deposition of coarse Mytilus-bank and Ostrea-bank units suggests that the establishment of the present dual-flow regime in the Bosphorus took place at about 4,400 ¹⁴C years B.P.