Isolation,purification and characterization of the hydrogen evolution promoting factor of hydrogenase ofSpirulina platensis

A component (s-factor) with obvious promoting effect on hydrogen evolution of hydrogenase has been isolated and extracted from a cell-free preparation ofSpirulina platensis. The effect of the s-factor in the reaction system is similar to that of Na₂S₂O₄, but is coupled with light. The s-factor has the maximum absorption peak at 620 nm in the oxidized state, at 590 nm in the reduced state. The partially purified s-factor showed two bands by SDS-PAGE and is distinctly different from phycocyanin, which has no change of oxidized state and reduced state absorption spectra, and also has no promoting effect on hydrogenase ofSpirulina platensis under the light. The research supported by the NSFC.     

Pathogenicity of vibrios in fish: An overview

Bacteria of the genus Vibrio are ubiquitously distributed in the marine environment. Due to the rapid expansion of intensive mariculture and the consequent deterioration of culture conditions, more and more Vibrio spp. have been recognized as pathogenic agents in outbreaks of vibriosis, a serious epizootic disease affecting most wild and farmed fish species worldwide, which has become the most important limiting factor for the development of intensive mariculture industry. Attempts have been made to understand the pathogenicity of vibrios in host fish with the ultimate aim of elucidating the best means for disease control. After an extensive literature survey of the recent advances in the field of fish vibriosis, the pathological changes, virulence factors and associated potential pathogenic mechanisms, transmission routes and related environmental factors involved in outbreak of vibriosis, as well as the controlling strategies are reviewed in the present paper.     

Using unmanned underwater vehicles as research platforms in coastal ocean studies

The advantages of using unmanned underwater vehicles in coastal ocean studies are emphasized. Two types of representative vehicles, remotely operated vehicle (ROV) and autonomous underwater vehicle (AUV) from University of South Florida, are discussed. Two individual modular sensor packages designed and tested for these platforms and field measurement results are also presented. The bottom classification and albedo package, BCAP, provides fast and accurate estimates of bottom albedos, along with other parameters such as in-water remote sensing reflectance. The real-time ocean bottom optical topographer, ROBOT, reveals high-resolution 3-dimentional bottom topography for target identification. Field data and results from recent Coastal Benthic Optical Properties field campaign, 1999 and 2000, are presented. Advantages and limitations of these vehicles and applications of modular sensor packages are compared and discussed.     

Use of microelectrodes for electrochemical measurement of nitric oxide in natural seawater

In this paper, the application of a homemade Nation and Co(Salen) modified platinum microelectrode and an ISO-NOPMC microsensor (World Precision Instruments, USA ) to measure nitric oxide in natural seawater is reported. These two microelectrodes are suitable for the measurement. In natural seawater, the sensitivity and stability of the ISO-NOPMC microsensor are higher than that of the homemade Nation and Co(Salen) modified platinum microelectrode.     

Preliminary study on PAH degradation by bacteria from contaminated sediments in Xiamen Western Sea,Fujian, China

In order to estimate the biodegradation of three polycyclic aromatic hydrocarbons (PAHs) compounds, bacterial strains were isolated from marine sediments in three heavily contaminated sites (Yuandang Lake, Dongdu Port and Aquacultural zones in Maluan Bay) in Xiamen Western Sea. The results show three bacterial strains, which used pyrene as the sole carbon source, were identified as strains of Aureobacterium sp.,Arthrobacter sp., Rhodococcus sp. The PAH-degrading bacteria isolated had a strong ability to degrade phenanhrene, fluoranthene and pyrene at different degradation rates. The highest degradation rate was observed when three PAH compounds were mixed with an individual strain in the medium. The three PAHs were degraded after one week with a degradation rate of 89.94 % for phenanthrene and 93.4 % for both of fluoranthene and pyrene.In addition, after 25 days of incubation, the degradation rate was 99.98 % for phenanthrene and 99.97 % for both of fluoranthene and pyrene. Optical density was measured to estimate bacterial growth during the degradation of PAHs. Highest levels of bacterial growth were observed with a three PAH mixture in the culture, suggesting that the concentration of PAHs influenced bacterial growth and the highest levels of degradation for most series were detected after one week of incubation.     

Influence of Headland Breakwaters on Morphological Processes at Longfengtou Beach in Haitan Bay,Facing the Taiwan Strait

Breakwaters can be used as artificial headlands in beach nourishment to mitigate coastal erosion. Longfengtou Beach, located on the southwest coast of Haitan Bay facing the Taiwan Strait on the northeast, suffers severe erosion, where the monsoon causes significant season hydrodynamic variations. Headland breakwaters are intended to be employed to mitigate coastal erosion. A 2D sediment transport model is established using MIKE21 based on current-wave coupling and calibrated by measured data. Summer and winter wave conditions are chosen as characteristic weak and strong waves respectively. The numerical results of suspended sediment concentration and seasonal morphological evolution are compared under the conditions with and without the headland breakwaters. Sediment transport in summer can be regarded as mainly determined by current field, while in winter wave effect is strong enough to change current field. The northern breakwater, nearly perpendicular to the ebb current direction, obstructs the currents and decreases velocity of the ebb currents, and confines the water carrying sediments within the protection area during the flood period. The southern breakwater also breaks the waves in advance and significantly reduces the hydrodynamic effects during the flood period and maintains high suspended sediment concentration in the protection area. In general, two headland breakwaters decrease the erosion near the beach and enlarge the deposition area, which play a significant role in prevention of sediment loss in nearshore area and mitigate coastal erosion.     

Assessing conservation practices in Amalacaxco Gorge (Izta-Popo National Park,Central Mexico) using fallout ~(137)Cs and Optically Stimulated Luminescence (OSL)

This study aims to assess conservation practices in Izta-Popo National Park(Central Mexico) by evaluating the mechanisms of sediment transfer. We applied a methodology based on fallout ~(137)Cs and optically stimulated luminescence(OSL) analysis. This was tested in the upper catchment of Amalacaxco Gorge, selected for being one of the sectors of the park in which man-made actions have been implemented in last decades to favor forest growth in the alpine grassland and to reduce the effect of water runoff. We quantified the ~(137)Cs activity using gamma and beta spectrometry of fine sediment grains extracted from the surface of parcels of 0.4 m2 in areas of natural forest, natural alpine grassland, alpine grassland with conservation practices, ravines and trails. In general, ~(137)Cs values increases as local slope decreases as it was expected. The natural forest is the most stable area in terms of soil erosion and sediment accumulation and, mean ~(137)Cs activity was taken as reference to assess cumulative zones, with higher ~(137)Cs values and erosive, with lower. We found that trails are accumulative surfaces but in other areas, erosion predominates. Man-made ditches, trenches and afforestation in the alpine grassland have higher ~(137)Cs values than thenatural grassland, which indicates that conservation practices are limiting the sediment transfer from hillslopes to channels, however, soil retention is less than in the natural grassland. Additionally, we evaluated the luminescence(OSL) values obtained from samples extracted from the sediment transported in ravines that are cutting into different sectors of the study area to assess the grade of resetting of fluvial materials. These luminescence results indicated that the sediment transported in ravines that are cutting into the natural forest and alpine grassland is bleached more efficiently than the sediment transported in the alpine grassland with conservation practices. Results of fallout ~(137)Cs and luminescence strongly suggest that man-made actions in this part of the Izta-Popo National Park are dramatically modifying the natural mechanisms of sediment transfer and favoring soil erosion. We conclude that made ditches, trenches and afforestation are not an effective conservation practice in Amalacaxco Gorge because they are promoting soil erosion instead of reducing it.     

Sex determination and differentiation in Aurelia sp.1: the absence of temperature dependence

Cnidarians, being regarded as ‘basal' metazoan animals, are considered to have relatively high plasticity in terms of sex reversal. In this study we used an experimental approach to demonstrate sexual diff erentiation and plasticity in benthic polyps and pelagic medusae of A urelia sp.1 maintained at dif ferent temperatures. Results indicated that in A urelia sp.1, sex diff erentiation has been determined at the polyp stage and that all medusae originating from a given polyp are, phenotypically, of the same sex. In addition, the sex of polyps budding from the same clone(either male or female) at dif ferent temperatures appears to be the same as that of the parent. The sex of medusae that had originated from a known-sex polyp was observed to remain the same as that of the parent, irrespective of differences in strobilation or rearing temperatures. These results indicate that the mechanism of sex determination of Aurelia sp.1. is not influenced by prevailing temperature regimes. A comparison of variability in terms of sexual plasticity of A urelia sp.1 with that of Hydrozoa and Anthozoa suggests that species characterized by a free-swimming medusa life stage have a high dispersal potential, which probably results in a lower rate of sex reversal.     

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