中国道路网引起的景观破碎格局及其对保护区的影响

道路的修建往往带来景观破碎、生态系统功能受损等后果。有关道路网络的生态影响格局及其影响因素,是当前道路生态学、地理空间分析等领域共同关注的问题。本文利用2015年全国道路网络数据,采用定量空间分析方法,刻画了当前中国铺装道路所引起的陆表景观破碎的格局特征,并分析了道路网络对自然保护区的干扰及与多变量的影响关系。结果表明：① 全国铺装道路的生态影响面积占陆地面积的10%,将陆表切割成3万多个斑块,并呈现小斑块数目众多,大斑块数目较少的分布特点;同时,陆表破碎化程度呈现明显的东西分异规律,空间格局与人口分布、经济发展水平等影响因素的地理分布规律趋同;② 全国约58%的自然保护区受到道路干扰,保护区的受干扰情况存在高等级保护区受干扰弱、低等级保护区次之、国家公园受干扰程度最强等差异特征,且国家公园处于受干扰高于未设保护区域的状态;③ 主要人类活动因素与保护区受干扰程度呈正相关,保护区面积、地形因素与受干扰程度呈负相关,具有面积小、受保护等级低、位于平原地区、气候适宜等特征的保护区更易受到道路干扰,处于已经受到人类活动破坏,生态脆弱的状态。因此,中国道路建设应追求社会发展与生态保护双平衡,同时道路干扰受到自然以及人文要素的共同作用,在相关影响机制研究和生态保护政策制定中应进行综合考虑。     

地理信息公共服务平台地理实体数据建模研究

围绕国家地理信息公共服务平台在线地理信息服务的需求,在分析国内外相关成果的基础上,提出了面向实体的数据建模思路,设计了地理实体数据模型,能够方便地实现地理信息与社会、经济、自然资源等专题信息的挂接,并有效解决多比例尺实体目标的一致性维护问题.     

拮抗香蕉枯萎病菌海洋细菌的筛选和鉴定

从93份中国南部海域近海海泥样品中分离出1800多株海洋细菌,以对峙培养法,测定海洋细菌对香蕉枯萎菌的拮抗作用,再以琼脂划线培养法(agar streak)测定菌株的抑菌活性,结果表明:约55.6%的菌株对香蕉枯萎菌有一定的拮抗作用,其中58株菌株抑制香蕉枯萎菌生长所形成的抑菌带宽度大于10.0mm,抑菌作用较好,复筛后其中13个菌株形成的抑菌带宽在15.0mm以上。经反复转接和对峙培养,筛选出拮抗效果好、抑菌活性稳定的菌株TC-1,初步鉴定为芽孢杆菌属的细菌,TC-1对14种病原微生物都有较好的抑菌效果。     

广西文蛤(Meretrix)的fAFLP 及ITS 分析

利用fAFLP标记技术和PCR-RFLP技术,对采集于广西的形态和壳色变异很大的2个文蛤群体(G、W)以及山东文蛤(S)群体进行了遗传结构和亲缘关系研究.fAFLP分析显示:在457个总扩增位点中存在53个W群体的特异性位点(其中9个为100%显性)、21个G群体的特异性位点、14个S群体的特异性位点,这些位点可作为群体鉴别的特征性标记;通过计算群体间相对遗传距离和聚类分析,发现S和G间的遗传距离最小(0.0424),在系统树上首先聚在一起,而W与S、G的相对遗传距离均很大(0.2308和0.2305),单独分出一支.对文蛤核糖体ITS的PCR-RFLP结果显示:W的三个不同壳色类群(WX、WC、WH)的酶切结果完全一致,而与G和S群体差别较大;G和S群体基本一致,但也稍有差别.进一步的ITS序列分析显示:W的ITS序列长度为1266-1269bp.而G和S分别为1614bp和1520bp;W群体内部序列比较保守,而与G和S群体之间在ITSl和ITS2开始和结尾处均存在多处插入/缺失;在依据序列构建的系统树上,WX、WC、WH三个类群聚在一起,S和G聚为另一支,两支相距甚远.本研究结果表明,fAFLP标记分析、PCR-RFLP分析和ITS序列分析结果一致,W群体和G、S群体的差异较大,已超出种内群体间的遗传变异,S和G尽管存在遗传分化,但仍应同属Meretrix meretrix-个物种.     

黄海冷水团海域微型异养鞭毛虫对异养细菌和蓝细菌摄食作用的初步研究

2006年10月在黄海冷水团海域的三个站点开展了微型异养鞭毛虫、异养细菌和蓝细菌的密度和生物量调查,进行了微型异养鞭毛虫的现场摄食实验,通过荧光标记细菌法和消化系数法获得该海区微型异养鞭毛虫对异养细菌和蓝细菌的摄食率,并估算了微型异养鞭毛虫对异养细菌和蓝细菌现存量及生产力的摄食压。结果显示,微型异养鞭毛虫、异养细菌和蓝细菌的密度分别为0.36×103~1.13×103,0.39×106~1.13×106和0.04×104~3.74×104cells/cm3,温跃层以上明显高于底层。微型异养鞭毛虫对异养细菌的摄食率为5.33~14.89个/(HF·h),对蓝细菌的摄食率为0.26×102~23.10×10-2cells/(HF·h),摄食率随深度而下降。微型异养鞭毛虫每天能消耗9.27%~33.08%的异养细菌现存量和8.12%~16.09%的蓝细菌现存量。同时,微型异养鞭毛虫对异养细菌和蓝细菌的日摄食量各占它们生产力的2.66%~13.10%和8.12%~16.09%。研究表明微型异养鞭毛虫的摄食可能不是秋季黄海冷水团海域浮游细菌及其生产力的主归宿。     

Aequorea taiwanensis n. sp. (Hydrozoa, Leptomedusae) and mtCOI sequence analysis for the genus Aequorea

Aequorea taiwanensis, a new hydrozoan species from the Taiwan Strait was described using morphological and molecular characteristics. Both morphological and mitochondrial cytochrome oxidase subunit I (mtCOI) data supported A. taiwanensis n. sp. as a valid species. Sequence divergence and genetic distance of A. taiwanensis n. sp., A. papillata and A. conica were analysed based on the mtCOI gene sequences. The mtCOI sequences from these three species of the genus Aequorea showed high variation frequency, with sequence divergences ranging from 9.10% to 11.9%, and pairwise genetic distances ranging from 0.097 to 0.130. MtCOI sequence analysis provided diagnostic molecular systematic characteristics for accurate identification and discrimination of the species of Aequorea or their populations, and will be used to resolve evolutionary relationships among them.It was suggested that 10%—20% pairwise mtCOI sequence differences indicated the specieslevel divergence among congeneric species in the Hydromedusae.     

Detection of nighttime sea fog/stratus over the Huanghai Sea using MTSAT-1R IR data

A dual channel difference (DCD) method is applied to detect nighttime sea fog/stratus over the Huanghai Sea using the infrared (IR) data of shortwave (3.5–4.0 μm) and longwave (10.3–11.3 μm) channels from the Multi-functional Transport Satellite (MTSAT)-1R, i.e., shortwave minus longwave brightness temperature difference (SLTD). Twenty-four sea fog events over the Huanghai Sea during March to July of 2006 and 2007 are chosen to determine a suitable value of SLTD for nighttime sea fog/stratus detection, and ...     

A preliminary study of variations of the Changjiang Diluted Water between August of 1999 and 2006

A large area hypoxia has been already reported respectively by two interdisciplinary surveys off the Changjiang Estuary since summer of 1999 and 2006. The hypoxic zone shows distinct year-to-year variations. Observed oceanographic data are first analysized and reveal a big difference for the Changjiang Diluted Water (CDW) between these two periods. These great changes are related to the tremendous reduction of the freshwater discharge and variations of wind fields between these two years. It is also found that the monthly mean intrusion of Kuroshio and its branches has increased in the northern East China Sea (ECS), but decreased in the southern ECS in August of 2006 as compared with 1999 on the base of general circulation models. Then, the Regional Ocean Modelling Systems is applied to the East China Sea to evaluate the contributions and relative importance of impacts from the river discharge, wind forcing and open boundary data. Our simulations reproduce the phenomena that more fresh water extends northeastward in 2006 and forms a negative SSS anomaly to the northeast of the river mouth as compared with 1999, which is consistent with observations. The five group numerical tests suggest that the wind forcing dominates the CDW variations followed by the Kuroshio and its branches. The study implies important roles played by hydrodynamic processes on the variability of hypoxic zone in the study areas.     

The study on a new technology of waterway information system

It is a lot limitations to survey and design waterway by the CAD technique,due to timely variation of hydrology and ecology in the complicated estuary area,such as Changjing River estuary.In this paper,the advantages of GIS technique is applied to manage and process the multi kind,multi scale and multi time hydrographic parameters,including water depth,current velocity,tidal,waves,wind speed,temperature and salinity etc.,also to deal with different projection,different scale charts.Meanwhile,in the paper,combination techniques of GIS and CAD has been developed for unification of the charts for efficient management,two-way automatic carts conversion between GIS and CAD,automatic cutting and assemble of a number of Charts,flight path of the projection of spatial data and coordinate transformation.This new technique has been applied to survey and design waterway of Changing River estuary.The result shows that the combination technology of GIS and CAD has application potentiality in the waterway survey and design.     

豹纹鳃棘鲈胚胎发育的初步观察

2008年5月20日,对海水网箱养殖的豹纹鳃棘鲈(Plectropomus leopardus Lacépède)亲鱼进行人工催产试验,并对获得的受精卵进行胚胎发育观察,描述其发育各期的形态特征及发育速度.豹纹鳃棘鲈的受精卵呈浮性,透明圆球形,卵径为816.5 μm±15.9 μm,具一直径约145.5 μm的大油球,有些受精卵还含有数个(1～6)小油球.在自然海区水温30.0～31.2℃条件下,胚胎发育时间为16 h32 min.