刍议枣庄因采矿引起的地质灾害及预防

1 枣庄矿产资源开采史 枣庄市矿产资源的开采有着悠久的历史.据当地甘泉寺的碑碣记载,1308年就有人在该地区挖窑采煤.13世纪中叶,民间采煤业逐渐兴盛起来.明代诗人满碧山"磨塘山欲尽,煤井地皆空"的诗句,就是对枣庄地区的采矿描述.开采陶土烧瓷的历史约有1400年;开采铁矿石手工冶铁也有500多年的历史.改革开放以来,枣庄地区的采矿业有了较快的发展,采矿品种由单一转向多种,现已达15个矿种.全市年产原煤2 000多万吨.县、乡(镇)、村办和个体办采石厂300多家,年采矿石量1 600万t,黏土、河砂等采矿点200余处,全市从事采矿业人员达16万余人.     

对虾传染性皮下及造血组织坏死病毒衣壳蛋白基因的克隆表达及抗体制备

本文根据GenBank登录的对虾传染性皮下及造血组织坏死病毒(IHHNV)的全基因组序列(NC-002190),设计出主要结构蛋白基因的相应引物,从厦门发病的对虾中,提取DNA,以此为模板,利用PCR扩增目的基因.再将目的基因分别与pGEX-6p1载体和pET-28a载体连接,构建重组表达载体pGEX-Ⅳ、pET-Ⅳ,重组子经酶切和测序鉴定后导入表达型大肠杆菌BL21,IPTG诱导表达,SDS-PAGE检测,大小约为62KDa和41KDa,与预测大小一致.将pET-Ⅳ表达重组蛋白经纯化,免疫小鼠,Western免疫印迹反应结果表明其抗体均可与pGEX-Ⅳ和pET-Ⅳ表达的蛋白发生特异性反应.本研究的结果为对虾IHHNV的快速检测及其免疫提供了研究基础.     

Tidal current observation in the southern Yellow Sea in the summers of 2001 and 2003

Direct current observations in the Yellow Sea interior are very scarce due to intense fishing and trawling activities. Most previous studies on tides in the area were based on coastal measurements or satellite altimeter sea levels and have not been rigorously compared with direct measurements. In this paper, tidal currents are studied with current profiles from three bottom-moored Sontek Acoustic Doppler Profilers （ADPs） deployed in the southern Yellow Sea in summer of 2001 and 2003. The measured current series were dominated by tidal currents. Maximum velocities are between 40-80 cm/s at the mooring stations. M2 current is the most dominant primary tidal constituent, while MS4 and M4 are the most significant shallow water tides with much smaller amplitudes than the primary tides.     

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Sequence analysis and quantitative detection of Norwalk-like viruses in cultured oysters of China

We isolated 4 Norwalk-like viruses (NLVs) contaminated oysters from 33 Chinese oysters collected from local commer-cial sources of Shandong Province. After amplification of the RNA-dependent RNA polymerase (RdRp) region of NLVs genomes with RT-PCR, the open reading frame 1 (ORF1) of the RdRp was sequenced and subjected to multiple-sequence alignment. The re-suits showed that NLVs in the four isolates belong to genogroup Ⅱ. The sequence comparison showed that the similarity between four Chinese oyster isolates were higher than 99.0%, which indicated that NLVs prevalent in close areas have high homogeneity in genome sequences. In addition, the most conserved sequences between diverse NLVs were used to design primers and TaqMan probes, then the real-time quantitative PCR assay was performed. According to the standard curve of GII NLVs, the original amounts (copies) of NLVs in positive patient's fecal isolate, positive Japanese oyster isolate, and the Chinese oyster isolate were 8.9×108, 1.25×108 and 4.7×101 respectively. The detecting limit of NLVs was 1×101 copies. This study will be helpful for routine diagnosis of NLVs pathogens in foods and thus for avoiding food poisoning in the future.     

江苏省“十一五”省级基础测绘规划实施方案研究

在分析了江苏基础测绘状况的基础上,介绍了"十一五"基础测绘的原则、目标和任务,提出了组织实施方案和保障措施.     

基于星敏感器的航天器姿态估计

星敏感器是一种高精度的姿态测量仪器,本文在分析星敏感器姿态测量原理的基础上,利用序贯平差方法确定航天器姿态并予以实现.仿真结果表明,该算法能满足实时姿态确定的精度要求,且降低了数据存储量,节省了存储单元,具有较强的灵活性.     

超导重力仪GWR-TT70在武昌站的测量结果及其长期稳定性分析

介绍了超导重力仪 GWR- TT70 ( 1986- 0 1～ 1993- 12 )在武昌站的观测数据及潮汐参数的计算结果。采用了非数字滤波调和分析法 ,计算出该仪器观测重力值对气压的响应系数和长周期波潮汐参数 ,得到潮汐因子在 8年中的稳定度为 0 .5%以内。最后分析了仪器漂移特征和武昌站重力长期变化的可能量级。     

认识氡气保护健康

高浓度氡的长期照射导致受照人群的肺癌发病率增加已被世界卫生组织、国际放射防护委员会等机构所公认,因此,人们对室内居住环境中的放射性越来越关心和重视.     

基于水利益共享的跨境流域水资源多目标分配研究——以澜沧江-湄公河为例

以水利益共享代替分水的理念有利于充分发挥水资源效益和减少区域矛盾冲突,但由于缺乏具体可实施的分配模式一直停留在设想阶段。基于水利益共享理念,建立跨境流域水资源多目标分配指标体系,并结合澜沧江-湄公河流域跨境水资源利用现状及需求,提出澜沧江-湄公河流域跨境水资源多目标分配模型。为基于水利益共享的跨境水资源多目标分配提供了具有充分可操作性的指标体系和分配模型,有助于推进跨境流域水利益共享的实施,实现区域双边或多边在水资源利用上的共赢和发展目标。