Cloning and sequence analysis of a full-length cDNA of <Emphasis Type="Italic">SmPP1cb</Emphasis> encoding turbot protein phosphatase 1 beta catalytic subunit

Reversible protein phosphorylation, catalyzed by protein kinases and phosphatases, is an important and versatile mechanism by which eukaryotic cells regulate almost all the signaling processes. Protein phosphatase 1 （PP1） is the first and well-characterized member of the protein serine/threonine phosphatase family. In the present study, a full-length cDNA encoding the beta isoform of the catalytic subunit of protein phosphatase l（PPlcb）, was for the first time isolated and sequenced from the skin tissue of flatfish turbot Scophthalmus maximus, designated SmPPlcb, by the rapid amplification of cDNA ends （RACE） technique. The cDNA sequence of SmPPlcb we obtained contains a 984 bp open reading frame （ORF）, flanked by a complete 39 bp 5＇ untranslated region and 462 bp 3＇ untranslated region. The ORF encodes a putative 327 amino acid protein, and the N-terminal section of this protein is highly acidic, Met-Ala-Glu-Gly-Glu-Leu-Asp-Val-Asp, a common feature for PP1 catalytic subunit but absent in protein phosphatase 2B （PP2B）. And its calculated molecular mass is 37 193 Da and pI 5.8. Sequence analysis indicated that, SmPPlcb is extremely conserved in both amino acid and nucleotide acid levels compared with the PPlcb of other vertebrates and invertebrates, and its Kozak motif contained in the 5＇UTR around ATG start codon is GXXAXXGXXATGG, which is different from mammalian in two positions A6 and G3, indicating the possibility of different initiation of translation in turbot, and also the 3＇UTR of SmPPlcb is highly diverse in the sequence similarity and length compared with other animals, especially zebraf＇lsh. The cloning and sequencing of SmPPlcb gene lays a good foundation for the future work on the biological functions of PP1 in the flatfish turbot.     

Landscape Dynamics and Driving Factors in Da＇an County of Jilin Province in Northeast China During 1956-2000

The landscape pattern of Da＇an County, Northeast China has undergone significant changes since the 1950s as a result of climatic change and human activities. The aim of this paper is to quantitatively study landscape pattern and its spatial dynamics of Da＇an County at the landscape level over the nearly 50-year span. Patch dynamics were examined according to land use and land cover change processes built from a series of images, as well as topographic maps, and temporal patterns built from landscape pattern metrics. The transition matrix of landscape patch types and changes of various landscape metrics were applied. The results showed that, from 1956 to 2000, the landscape within the study area had undertaken a complicated evolution in landscape structure and composition. The outstanding characteristic is that saline-alkali land increased and grassland decreased. As some smaller patches amalgamated, the heterogeneity of patch decreased. All those changes were the synthetic result of both climatic and anthropogenic influences, but the predominant factor was different in different parts. In the southern part of the study area, the landscape pattern changes resulted from the modification of climate obviously, while in the northern part, the landscape pattern changes were mainly caused by human activities, such as the conversion between farmland and saline-alkali land. This phenomenon showed that human activities played more important role in the north than in the south of Da＇an County.     

基于模糊控制的快速循迹智能车的设计与实现

提出基于模糊控制的快速循迹智能车的实现方法.利用转速传感器、线阵CCD、舵机等构成一个闭环控制系统,并采用PI算法控制智能车后轮速度.采用模糊控制算法将线阵CCD返回的误差模糊化,然后按照MAX-MIN规则进行逻辑推理,最后将推理结果反模糊化后以控制舵机转向.实验结果证明了该方法的有效性.     

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Study on the isotherms of the interaction between suspended particles and Cu (II) in the Huanghe River

The isotherms of the interaction between the suspended particles and Cu²⁺, and the effects of lysine and asparaginic acid on the isotherms in the Huanghe (Yellow) River were studied by applying the theory and method of interfacial stepwise ion/coordination particle exchange. We obtained a new stepped river isotherm, formed by two curves joined together with a “plateau” in the middle. The adsorption equilibrium constantsK ₁ andK ₂ were calculated by using the isothermal equation of surface stepwise ion exchange. Amino acid in small amount promotes exchange adsorption of the suspended particles with Cu²⁺. The degree of promotive action relates to the isoelectric point of amino acid. The promotive effect of lysine is bigger than that of asparaginic acid. Project 29361001 supported by NSFC.     

测绘技术进步状况和2010年定位方向预测分析

该文是对测绘技术进步对经济发展的作用进行定量分析,并运用经济、数学、统计等科学方法,依据产出增长数学方程,对测绘生产单位产值增长、技术进步速度和水平等进行测算,预测２０１０年测绘生产发展规模、技术水平等,提出对策建议,供管理决策者及部门参考。     

Hydrocarbon- Generating Model of the Area Covered With Volcanic Rock

The distribution of Oil & gas fields shows their close relationship with the most active tectonic regions. This is not a coincidence but having a scientific reasons. The crustal active regions, refer to the places where the active natural earthquake, volcanic activities, underground water happened, and the areas of the leaking off of natural gas to the surface of the crust. The magma of volcanic activities brings the organic "kitchen range body" hydrocarbon- generating model and inorganic genetic hydrocarbon to the regions covered by volcanic rock. Underground water brings a catalytic hydrocarbongenerating model for organic matter, and the leaking- off of H2 and CO2 contributes a synthetic hydrocarbon - generating model. Volcanic activities bring the assemblage of Source, Reservoir and Seal formed by the sediments and magma the sedimentary basins, and the hydrocarbon - generating system with a "water - volcano" binary structure is formed. All these conditions are favorable and excellent for the formation of oil & gas fields. The distribution of American oil & gas fields have very close relationship with the mines of Fe, Mn, Ct, Mo, W and V, deposits of Zn, Cu, V, Pb, Al and Hg, and the deposits of fluorite, sulfur, potassium salt, phosphate and halite, and the distribution of sulfate- chloride of river water. The reason why few oil & gas fields discovered in the regions covered by volcanic rock in western America maybe because of the view of "inconsistency between petroleum and volcano". Further more, It's very difficult to carry out a geophysical exploration in such kinds of regions.This paper examined a few hydrocarbon-generating models (systems) mentioned above and came up with some fresh ideas on the exploration in the areas covered with volcanic rocks.     

Paleogene evolution and demise of the proto‐Paratethys Sea in Central Asia (Tarim and Tajik basins): Role of intensified tectonic activity at ca. 41 Ma

The proto‐Paratethys Sea covered a vast area extending from the Mediterranean Tethys to the Tarim Basin in western China during Cretaceous and early Paleogene. Climate modelling and proxy studies suggest that Asian aridification has been governed by westerly moisture modulated by fluctuations of the proto‐Paratethys Sea. Transgressive and regressive episodes of the proto‐Paratethys Sea have been previously recognized but their timing, extent and depositional environments remain poorly constrained. This hampers understanding of their driving mechanisms (tectonic and/or eustatic) and their contribution to Asian aridification. Here, we present a new chronostratigraphic framework based on biostratigraphy and magnetostratigraphy as well as a detailed palaeoenvironmental analysis for the Paleogene proto‐Paratethys Sea incursions in the Tajik and Tarim basins. This enables us to identify the major drivers of marine fluctuations and their potential consequences on Asian aridification. A major regional restriction event, marked by the exceptionally thick (≤ 400 m) shelf evaporites is assigned a Danian‐Selandian age (ca. 63–59 Ma) in the Aertashi Formation. This is followed by the largest recorded proto‐Paratethys Sea incursion with a transgression estimated as early Thanetian (ca. 59–57 Ma) and a regression within the Ypresian (ca. 53–52 Ma), both within the Qimugen Formation. The transgression of the next incursion in the Kalatar and Wulagen formations is now constrained as early Lutetian (ca. 47–46 Ma), whereas its regression in the Bashibulake Formation is constrained as late Lutetian (ca. 41 Ma) and is associated with a drastic increase in both tectonic subsidence and basin infilling. The age of the final and least pronounced sea incursion restricted to the westernmost margin of the Tarim Basin is assigned as Bartonian–Priabonian (ca. 39.7–36.7 Ma). We interpret the long‐term westward retreat of the proto‐Paratethys Sea starting at ca. 41 Ma to be associated with far‐field tectonic effects of the Indo‐Asia collision and Pamir/Tibetan plateau uplift. Short‐term eustatic sea level transgressions are superimposed on this long‐term regression and seem coeval with the transgression events in the other northern Peri‐Tethyan sedimentary provinces for the 1st and 2nd sea incursions. However, the 3rd sea incursion is interpreted as related to tectonism. The transgressive and regressive intervals of the proto‐Paratethys Sea correlate well with the reported humid and arid phases, respectively in the Qaidam and Xining basins, thus demonstrating the role of the proto‐Paratethys Sea as an important moisture source for the Asian interior and its regression as a contributor to Asian aridification.