A simple shaker table for seismometer calibration

A unique and simple shaker table (shake table or shaking table), designed, constructed, and installed at the Hawaii Institute of Geophysics, has proven to be a valuable aid in testing and calibrating short period seismometers, as well as ocean bottom and ocean sub-bottom seismometer/tilt meter packages. It consists of a platform suspended in a stairwell by a single elastic cord (10 m extended length) driven by GeoSpace HS-10 geophones. Platform motion is monitored by orthogonal reference geophones and tilt meters. The relatively low natural periods of the platform, about 1.9 sec vertical and 6.5 sec horizontal, provide sufficient isolation from local vibrations that calibration can be made near operational amplitudes. Vertical or horizontal driver geophones can be driven by a commercial signal generator or white noise generator, or from magnetic tape output. The table can also be tilted with respect to the drivers to determine tilt tolerances and to calibrate tilt meters. A Hewlett-Packard 3582-A spectrum analyzer, used to analyze both reference and output signals, provides near real-time system cabibration and is an efficient means for investigating parasitic system resonances. The analyzer can also provide a white noise signal source to the driver geophones.Hawaii Institute of Geophysics Contribution 1443.     

Coulometric total carbon dioxide analysis for marine studies: maximizing the performance of an automated gas extraction system and coulometric detector

A global survey of the distribution of dissolved CO₂ taking advantage of sampling opportunities provided by the World Ocean Circulation Experiment: World Hydrographic Program (WOCE-WHP) is being carried out through 1995. Goals include the measurement of oceanic inorganic carbon transport and the development of a data base from which future fossil-fuel CO₂ build-up can be monitored. The analytical method selected for total carbon dioxide (C_T) is gas extraction of acidified seawater with coulometric titration of the acid formed by the resultant carbon dioxide and monoethanolamine. To combine high accuracy and precision (± 1.5 μmol/kg for C_T ≥ 2000 μmol/kg) with a high rate of analysis, we have modified an automated single-parameter system. Following prototype development between 1987 and 1990, an instrument emerged with the acronym Somma standing for single-operator multiparameter metabolic analyzer. Improved functional and operating procedures have integrated electronic calibration, CO₂ gas calibration, and sample analysis with automated pressure, temperature, and conductivity (salinity) sensing into a single convenient transportable package.     

Cloning of the major 3-MC inducible phase I and phase II detoxification enzymes of plaice liver

cDNA's coding for cytochrome P4501A1 (CYP1A1), phenol UDP-glucuronosyltransferase (UDPGT) and glutathione S-transferase (GST-A) were cloned and sequenced from an expression library prepared from the liver of a 3-methylcholanthrene (3-MC) induced plaice. Plaice CYP1A1 and Phenol UDPGT display a high degree of structural conservation with homologous mammalian isoforms and their mRNAs were shown to be highly induced in liver after 3-MC treatment of fish. Expression of plaice GST-A, which displays closer homology to GSTs from plants and invertebrates than those of mammals, is repressed after 3-MC treatment.     

Seasonal variations in the speciation of dissolved iodine in the Chesapeake Bay

The speciation of dissolved iodine and the distributions of the iodine species in the deep Chesapeake Bay underwent seasonal variations in response to changes in the prevailing redox condition. In the deep water, the ratios of iodate to iodide and iodate to inorganic iodine decreased progressively from the Winter through the Summer as the deep water became more poorly oxygenated before they rebounded in the Fall when the deep water became re-oxygenated again. The composition of the surface water followed the same trend. However, in this case, the higher biological activities in the Spring and the Summer could also have enhanced the biologically mediated reduction of iodate to iodide by phytoplankton and contributed to the lower ratios found during those seasons. Superimposed on this redox cycle was a cycle of input and removal of dissolved iodine probably as a result of the interactions between the water column and the underlying sediments. Iodine was added to the Bay during the Summer when the deep water was more reducing and removed from the Bay in the Fall when the deep water became re-oxygenated. A third cycle was the inter-conversion between inorganic iodine and ‘dissolved organic iodine’, or ‘‘DOI’’. The conversion of inorganic iodine to ‘DOI’ was more prevalent in the Spring. As a result of these biogeochemical reactions in the Bay, during exchanges between the Bay and the North Atlantic, iodate-rich and ‘DOI’-poor water was imported into the Bay while iodide- and ‘DOI’-rich water was exported to the Atlantic. The export of iodide from these geochemically reactive systems along the land margins contributes to the enrichment of iodide in the surface open oceans.     

Nitrate reductase activity and the speciation of selenium at the mouth of Chesapeake bay

Nitrate reductase activity and the concentrations of selenite and selenate were monitored for six months at the mouth of Chesapeake Bay (USA). A positive correlation was found between nitrate reductase activity and the concentration of selenite, suggesting that selenite may be formed in coastal waters and nitrate reductase may be involved in the process.     

Voltammetric estimation of iron(III) thermodynamic stability constants for catecholate siderophores isolated from marine bacteria and cyanobacteria

Thermodynamic stability constants have been estimated for the complexation of iron(III) with catecholate-type siderophores isolated from the marine bacterium Alteromonas luteoviolacea and from the marine cyanobacterium Synechococcus sp. PCC 7002. Stability constants were determined utilizing the “chelate scale” of Taylor et al. (1994). The scale is based upon a linear relationship between the reduction potentials and the pH-independent thermodynamic stability constants for known iron(III) complexes. Log K values for the alterobactin B ferric iron complex are 43.6 ± 1.5 at pH 8.2 and 37.6 ± 1.2 at pH 6, consistent with a shift from bis-catecholate to monosalicylate/monocatecholate iron coordination with decreasing pH. Synechococcus isolates PCC 7002 Nos. 1 and 3 formed iron(III) complexes with stability constants of approximately 38.1 ± 1.2 and 42.3 ± 1.5, respectively. The binding strengths of the iron(III) complexes examined in this study are quite high, suggesting that catecholate siderophores may play a role in the solubilization and biological uptake of iron in the marine environment.     

Abundance of the introduced seastar, Asterias amurensis, and spatial variability in soft sediment assemblages in SE Tasmania: Clear correlations but complex interpretation

The northern Pacific seastar, Asterias amurensis, was first collected in southeast Tasmania in 1986. Mistaken for the endemic asteroid Uniophora granifera, its true identity was not realised until 1992. It is now a conspicuous predator in soft sediment habitats in this region, and is considered a major threat to native assemblages and commercial species. We examined the structure of soft sediment assemblages at different spatial scales in southeast Tasmania, and correlated spatial variation in community composition with seastar abundances. We found that the structure of soft sediment assemblages is highly variable at a range of spatial scales from metres to tens of kilometres. Clear differences in the composition of assemblages and abundances of major taxa were detected between areas with and without seastars and between areas with low and high seastar densities. However, the nature of these patterns suggests that they are more likely due to differences in sediment characteristics than due to impacts of the seastar. Thus, spatial differences in soft sediment assemblages might have been erroneously attributed to seastars without detailed information on important physical factors such as sediment characteristics. A second survey, using larger sampling units (1 m²) but across a more limited spatial extent, targeted bivalves and heart urchins that were identified as important prey of the seastar in observations of feeding and in experimental studies. Large-scale patterns of abundance and size structure were consistent with seastar effects anticipated from small-scale experimental and feeding studies for some, but not all, species. While the field survey ultimately provided evidence about the presence or absence of seastar impacts at large-scales, the identification of key ecological variables in experimental and feeding studies proved crucial to both the design and interpretation of patterns observed in the large-scale surveys. Overall, this work highlighted the necessity to consider multiple lines of evidence rather than relying on a single ‘inferential’ test, in the absence of pre-impact data.     

Manganese and iron distributions off central California influenced by upwelling and shelf width

In July 2002, a combination of underway mapping and discrete profiles revealed significant along-shore variability in the concentrations of manganese and iron in the vicinity of Monterey Bay, California. Both metals had lower concentrations in surface waters south of Monterey Bay, where the shelf is about 2.5 km wide, than north of Monterey Bay, where the shelf is about 10 km wide. During non-upwelling conditions over the northern broad shelf, dissolvable iron concentrations measured underway in surface waters reached 3.5 nmol L⁻¹ and dissolved manganese reached 25 nmol L⁻¹. In contrast, during non-upwelling conditions over the southern narrow shelf, dissolvable iron concentrations in surface waters were less than 1 nmol L⁻¹ and dissolved manganese concentrations were less than 5 nmol L⁻¹. A pair of vertical profiles at 1000 m water depth collected during an upwelling event showed dissolved manganese concentrations of 10 decreasing to 2 nmol L⁻¹, and dissolvable iron concentrations of 12–20 nmol L⁻¹ in the upper 100 m in the north, compared to 3.5–2 nmol L⁻¹ Mn and 0.6 nmol L⁻¹ Fe in the upper 100 m in the south, suggesting the effect of shelf width influences the chemistry of waters beyond the shelf.These observations are consistent with current understanding of the mechanism of iron supply to coastal upwelling systems: Iron from shelf sediments, predominantly associated with particles greater than 20 μm, is brought to the surface during upwelling conditions. We hypothesize that manganese oxides are brought to the surface with upwelling and are then reduced to dissolved manganese, perhaps by photoreduction, following a lag after upwelling.Greater phytoplankton biomass, primary productivity, and nutrient drawdown were observed over the broad shelf, consistent with the greater supply of iron. Incubation experiments conducted 20 km offshore in both regions, during a period of wind relaxation, confirm the potential of these sites to become limited by iron. There was no additional growth response when copper, manganese or cobalt was added in addition to iron. The growth response of surface water incubated with bottom sediment (4 nmol L⁻¹ dissolvable Fe) was slightly greater than in control incubations, but less than in the presence of 4 nmol L⁻¹ dissolved iron. This may indicate that dissolvable iron is not as bioavailable as dissolved iron, although the influence of additional inhibitory elements in the sediment cannot be ruled out.