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861.
862.
The marine microalga Nannochloropsis sp. contains various elongases and desaturases that are critical for biosynthesis of polyunsaturated fatty acids. A full-length cDNA encoding a long-chain fatty acid elongase, named Ns FAE, was cloned from Nannochloropsis sp.. The open reading frame of Ns FAE(GenBank accession no. MF680548) consisted of 1068 bp and encoded a predicted protein of 355 amino acids with molecular mass 38.8 k Da. The deduced polypeptide showed 43%–44% identity to fatty acyl elongases from other algae. RT-PCR experiments indicated that the Ns FAE gene exhibited the highest expression in Nannochloropsis sp. at 72 h(i.e., during the third growth stage) and the expression was significantly lower in the other four growth stages. Plasmid p Ns FAE-CRISPR and a recombinant DNA fragment(ADH1p-Ns FAE-CYCt) were transformed into Saccharomyces cerevisiae strain BY4742 using the CRISPR-Cas system. Yeast transformants containing Ns FAE produced three fatty acids not normally present in wild-type BY4742-linoleic acid, linolenic acid and eicosadienoic acid-indicating that Ns FAE encodes a functional elongase enzyme. 相似文献
863.
对典型的地震预警与烈度速报方法进行分析,提出了基于非对称传感器的地震预警与烈度速报综合用烈度仪,其由非对称结构的三分量传感器和基于ARM嵌入式系统的专用数据采集器组成。经过测试,该地震烈度仪的专用数据采集器具有大于107 dB的动态范围;其非对称三分量传感器结构中,所选的单分量B类传感器的加速度误差小于0.8%,动态范围大于105 dB,而所选的三分量C类传感器的加速度误差小于5%,动态范围大于61 dB。该地震烈度仪的各项指标均满足地震预警与烈度速报综合应用的各项技术要求,且功耗低、成本低,适合于高密度布设。 相似文献
864.
Ascertaining the relationship between "source-sink" landscape and non-point source(NPS) pollution is crucial for reducing NPS pollution, however, it is not easy to realize this target on cell unit scale. To reveal the relationships between "sourcesink" landscape and NPS pollution based on cell units of a small catchment in the Three Gorges Reservoir Region(TGRR), the runoff and nutrient yields were simulated first by rainfall events on a cell unit scale based on the Annualized AGricultural Non-Point Source Pollution Model(AnnAGNPS). Landscape structure and pattern were quantified with "sourcesink" landscape indicators based on cell units including landscape area indices and locationweighted landscape indices. The results showed that:the study case of small Wangjiagou catchment highlighted a good prediction capability of runoff and nutrient export by the AnnAGNPS model. Throughout the catchment, the spatial distribution trends of four location-weighted landscape indices were similar to the trends of simulated total nitrogen(TN) and total phosphorus(TP), which highlighted the importance of spatial arrangement of "source" and "sink" landscape types in a catchment when estimating pollutant loads. Results by Pearson correlation analysis indicated that the location-weighted landscape index provided a more comprehensive account of multiple factors, and can better reflect NPS-related nutrient loss than other landscape indices applied in single-factor analysis. This study provides new findings for applying the "source-sink" landscape indices based on cell units in small catchments to explain the effect of "source-sink" landscape on nutrient export based on cell unit, and helps improve the understanding of the correlation between "source-sink" landscape and NPS pollution. 相似文献
865.
The maximum normal impact resultant force(NIRF)is usually regarded as the sum of the static earth pressure of the dead zone and the dynamic impact pressure of the flowing layer.The influence of the interaction between the flowing layer and dead zone on the impact force is ignored.In this study,we classified two impact models with respect to the pileup characteristics of the dead zone.Then,we employed the discrete element method to investigate the influences of the pileup characteristics on the impact force of dry granular flow on a tilted rigid wall.If the final pileup height is equal to the critical value,the maximum NIRF can be estimated using a hydrostatic model,because the main contribution to the maximum NIRF is the static earth pressure of the dead zone.If the final pileup height is less than the critical value,however,the particles in the dead zone are squeezed along the slope surface by the impact ofthe flowing layer on the dead zone,and because of shear effects,the flowing layer causes an entrainment in the dead zone.This results in a decrease in the volume of the dead zone at the moment of maximum NIRF with increases in the slope angle.As such,the maximum NIRF mainly comprises the instant impact force of the flowing layer,so hydro-dynamic models are effective for estimating the maximum NIRF.Impact models will benefit from further study of the components and distribution of the impact force of dry granular flow. 相似文献
866.
Wetland plant communities in the plateau lakes of Yunnan Province, China, have decreased significantly over the past decades. To better understand this degradation, we analyzed the processes and characteristics of changes in wetland plant communities in two of the largest lakes in Yunnan Province, Dianchi and Erhai lakes. We collected records of native and alien plant communities in the two lakes from literature published from the 1950s to current period. We calculated plant community types and their area in some historical periods when related data were reported, and analyzed the relationship between changes in plant communities and water pollution. In Dianchi Lake, 12 community types of native plant communities, covering over 80% of the surface in the 1950s and 1960s, were reduced to four types covering 2.4% by the late 2000s. Alien plant communities started to appear in the lake in the late 1970s, and have since come to cover 4.9% of the lake surface, thereby becoming dominant. In Erhai Lake, 16 community types of native plant communities, covering 47.1% of the lake surface in the late 1970s, declined to 10 community types, covering 9.3% of the surface, by the late 2000s. Alien plant communities appeared in the middle 1980s, and at present cover 0.7% of the surface area. It was indicated that changes in plant communities were significant related to water eutrophication. The area occupied by native and alien plant communities was, respectively, negatively and positively related to the content of nutrients in water. This showed lacustrine pollution played an important role in native plant loss and alien plant invasion in the two plateau lakes. 相似文献
867.
Xiao-Pan Li Yu-Hui Luo Hai-Yan Yang Cheng Yang Yan Cai Hai-Tao Yang Li Zhou Yu-Qiong Shan 《Astrophysics and Space Science》2018,363(8):169
We report a ~6.1 yr quasi-periodicity for the blazar S5 0716+714 using the radio light curves at 4.8, 8 and 14.5 GHz observed by the University of Michigan Radio Astronomical Observatory (UMRAO) from 1981 to 2012, by means of the Jurkevich, discrete correlation function (DCF) and power spectral analysis techniques. There are a general correlation among light curves at different frequencies and a time lag of \(170\pm 10\) days between 4.8 and 14.5 GHz light curves can be confirmed. We also estimate the orbit parameters assuming a binary black hole system, and the magnetic field strength under the jet comoving frame. 相似文献
868.
869.
Comparisons of Infrared Colors and Emission-line Intensities between Two types of Seyfert 2 Galaxies
We study the relation between the infrared colors[ OⅢ] emission lines,gaseous absorbing column density(NH) ,and the detectability of the polarized (hidden)broad-line region(HBLR) in a large sample of 75 Seyfert 2 galaxies(Sy2s).From the indicators of star-formation activity,f60/f100 and LFIR/LB,we find some evidence that the Sy2s without HBLR show higher star-formation activities than those with HBLR,in agreement with previous prediction.Also,we confirm that the HBLR Sy2s tend to have a larger luminosity ratio of the core to the host galaxy,suggesting that the HBLR Sy2s display more powerful AGN activity.Howerver,the level of obscuration found in previous papers in nearly indistinguishable between the two types of Sy2s.The results support the statement that the non-HBLR Sy2s,with a weaker core component and a stronger star-formation activity component,are intrinsically different from the HBLR Sy2s,which are Sy1 systems with a hidden powerful AGN core and a low star-formation activity.The indications are that the non-HBLR Sy2s might be at an earlier evolutionary phase than the HBLR Sy2s. 相似文献
870.