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11.
蛤蜊科3种贝类16SrRNA基因片段及ITS2核苷酸序列分析 总被引:4,自引:0,他引:4
利用PCR技术分别扩增连云港及启东沿海蛤蜊科的西施舌(Coelomactra antiquata)、中国蛤蜊(Mactra chinensis)和四角蛤蜊(Mactra veneriformis)3种双壳贝的16SrRNA基因片段和ITS2核苷酸序列.测序后用DNAstar软件分析了核苷酸差异。结果显示:三种贝类16SrRNA基因片段长度相同,均为306bp(去除引物).核苷酸存在多态性。共有45个变异位点,54个核苷酸发生了变异。全部为碱基置换。西施舌与中国蛤蜊此片段核苷酸的同源性为88.9%.与四角蛤蜊的同源性为88.6%.中国蛤蜊与四角蛤蜊的同源性为90.6%。三种蛤蜊ITS2序列分别为390bp(西施舌)、441bp(四角蛤蜊)和466bp(中国蛤蜊)。存在长度多态性.ITS2核苷酸差异分析结果显示.西施舌与中国蛤蜊的同源性为70.9%-71.1%,西施舌与四角蛤蜊的为70.5%-71.0%。中国蛤蜊与四角蛤蜊的同源性为88.1%-88.8%。ITS2序列分析结果与16SrRNA基因片段分析结果一致.2种分子分析法均显示中国蛤蜊与四角蛤蜊的亲缘关系近。 相似文献
12.
介绍了马卡良 335分别发生于 1998年和 1999年的两次短时标光变。其中的一次是微光变 ,发生于 1999年 11月 2 0日 ,微光变区域的大小约 0 .80 7光时。还对微光变的可能机制进行了讨论 相似文献
13.
Pco2 of air and seawater samples from the East China Sea(ECS) were measured in situ in autumn, 1994,Ocean currents,terrestrial fluviation,biological activities,etc.,Pco2 char-acters in air and seawater were investigated,CO2 flux and its character in the East China Sea are discussed on the basis of the Pco2 profiles of air and seawater,It was clear that the nearshore was the source of CO2;and tht the oulter sea area was the sink of CO2; and that the shelf area of the EXS is a net sink for atmospheric CO2 in autumn. 相似文献
14.
应用给定换热器结构材料而使换热量最大的两侧换热表面的最佳匹配准则和使可用能损失率最小的最佳运行参数准则。利用两个准则间的关系 ,采用迭代的方式完成换热器的优化设计 ,使换热器的设计达到材料省、换热效果好及运行费用低的目的 ,且能在设计阶段实现。 相似文献
15.
Jun Nishikawa 《Solar physics》1994,152(1):125-130
Spatially-resolved precise photometric observations of the whole Sun at wavelengths of 545nm (FWHM 40nm) were carried out by using the CCD solar surface photometer. Bright parts of photospheric network have contrast of several tenths of percent, and their contribution to the total irradiance is approximately half that of active region faculae. The solar irradiance variations estimated from sunspots, faculae and active network (contrast>0.3%) agreed with the ACRIM data. The quiet Sun irradiance used in the present results was different from the total irradiance at the solar minimum observed by the ACRIM, which indicates unmeasured components (contrast>0.1%) cause the 11-year cycle irradiance variation. 相似文献
16.
Abstract— Abundances and isotopic compositions of noble gases in metal and graphite of the Bohumilitz IAB iron meteorite were measured. The abundance ratios of spallogenic components in metal reveal a 3He deficiency which is due to the diffusive loss of parent isotopes, that is, tritium (Tilles, 1963; Schultz, 1967). The diffusive loss likely has been induced by thermal heating by the Sun during cosmic‐ray exposure (~160 Ma; Lavielle et al, 1999). Thermal process such as impact‐induced partial loss may have affected the isotopic composition of spallogenic Ne. The 129Xe/131Xe ratio of cosmogenic components in the metal indicates an enhanced production of epi‐thermal neutrons. The abundance ratios of spallogenic components in the graphite reveal that it contained small amounts of metal and silicates. The isotopic composition of heavy noble gases in graphite itself was obtained from graphite treated with HF/HCl. The isotopic composition of the etched graphite shows that it contains two types of primordial Xe (i.e., Q‐Xe and El Taco Xe). The isotopic heterogeneity preserved in the Bohumilitz graphite indicates that the Bohumilitz graphite did not experience any high‐temperature event and, consequently, must have been emplaced into the metal at subsolidus temperatures. This situation is incompatible with an igneous model as well as the impact melting models for the IAB‐IIICD iron meteorites as proposed by Choi et al. (1995) and Wasson et al (1980). 相似文献
17.
An improved approach is given for deriving the physical parameters of a molecular cloud by its NH3 rotation-inversion hyperfine spectra. The optical depth τ0(1, 1) of NH3 (1,1) is obtained by considering the blending effect of the magnetic hyperfine spectral lines, the (1-1) excitation temperature Tex (1, 1) is calculated by a two level model, the effect of different collision rate and the thermalization of the (1, 1) inversion lines are discussed, the rotation temperature TR ( 2, 1) between the NH3 levels (2, 2) and (1, 1) and the column density N( 1, 1) of the NH3 (1, 1) inversion level are derived, the results of the total column density from different assumptions for the abundance ratio of ortho-NH3 and para-NH3 are also discussed.
This approach is used for the molecular cloud NGC 2023 by using the relevant observed data and its optical depth and other physical parameters are obtained. 相似文献
18.
19.
月日潮汐摩擦和地球惯量矩变化是日长长期变化的主要原因.在本文中,利用最新的地球物理和古生物钟数据,对过去15亿年以来的月日潮汐摩擦、地球惯量矩变化和日长长期变化等作了数值对比研究.由此得到二个重要结论:一是仅利用地球的自转形变不能解释J2的变化,这说明地球的重力分异现象至今仍存在着;其二是在几亿年前的潮汐摩擦比现在大得多,若取潮汐耗散与距离的立方成反比时,理论结果与由古生物钟得到的回归年日数和朔望月日数数据较为符合。 相似文献
20.