Expression of the Phycoerythrin Gene of Gracilaria lemaneiformis (Rhodophyta) in E. coli and Evaluation of the Bioactivity of Recombinant PE

Phycoerythrin (PE) is one of the most important proteins involved in light capturing during photosynthesis in red algae. Its potential biological activities had gained wide concerns. In the present study, tumor cytotoxic and hydroxyl radical assay were preformed to detect the bioactivity of recombinant PE. Recombinant plasmids pGEX-PE and pBGL were transformed into E.coli BL21 to make two recombinant strains BEX (pGEX-PE) and BGL (pBGL). PE expressing in BEX (pGEX-PE) was validated by SDS-PAGE and Western blotting analysis. SDS-PAGE analysis indicated that the PE-GST fusion protein was mostly inclusion bod- ies. Specific expression of PE was confirmed by Western blotting analysis. The recombinant E. coli BEX (pGEX-PE) cells were col- lected and sonicated. The supernatants were reserved for the tumor cytotoxic experiments. The result of tumor cytotoxic assay indi- cated that the supernatants containing PE had the activity of inhibiting the growth of Hela cells and with the increase of protein con- centration, the inhibiting rate increased from 37.31% to 63.26%, which showed significant difference from the control. Hydroxyl radical scavenging effect was tested with supernatants of BEX (pGEX-PE) and BGL (pBGL) cell lysates treated with sonication and heating. For the sonication samples, the scavenging rates of the supernatants of BEX (pGEX-PE) and BGL (pBGL) cell lysates were significantly higher than the negative control BL21(pGEX-4T) (P<0.02), and the scavenging rates increased slowly following the increase of the protein content. For the heating samples, except for the 0.2 mg mL-1 BGL (pBGL) products, the scavenging effects of the supernatants of BEX (pGEX-PE) and BGL (pBGL) cell lysates were stronger than that of negative control BL21(pGEX-4T). However, the effect intensity was not positively correlated with the increase of the protein concentration. Though a partially de- creased hydroxyl radical scavenging activity was led by heating, the biological activity was still retained and conspicuous. This re- search showed that phycoerythrin protein expressing in E. coli has the potential medical and sanitarian value.     

A Metallogenic Model of Gold Deposits of the Jiaodong Granite-Greenstone Belt

An analysis of trace elements and isotopic geochemistry suggest that the ore-forming materials of gold deposits in the Jiaodong granite-greenstone belt have multiple sources, especially the mantle source. Seismic wave, magnetic and gravity fields show that the crust-mantle structure and its coupling mechanism are the fundamental dynamic causes for the exchange and accumulation of materials and energy in the metallogenic system. Considering the evolution history of the structural setting, the tectono-metallogenic dynamics model of the area can be summarized as follows: (1) occurrence of the greenstone belt during the Archean-Proterozoic-the embryonic form of Au-source system; (2) stable tectonic setting in the Paleozoic-an intermittence in gold mineralization; (3) intensive activation and reformation of the greenstone belt in the Mesozoic-tectono-mineralization and tectono-diagensis; (4) posthumous structural activity in the Cenozoic-destruction of orebodies in the later stage. In the middle and late Ind     

震后快速钻探:历史、现状与未来

大地震发生后立即在断层带上进行钻探可以帮助我们获取更多的地震信息,尤其是可获得决定断层动态破裂的摩擦水平和强度,观测断层的愈合过程及可能触发余震的应力变化,并可获取控制破裂过程的重要的物理和化学属性。在2008年11月由国际大陆科学钻探项目（ICDP）和南加州地震中心（SCEC）联合在日本东京举办的为期3天的“震后快速钻探：历史、现状与未来”研讨会上,     

大别造山带140～85 Ma热窿伸展作用

区域热年代和差异冷却特性表明, 140～85 Ma大别造山带进入热窿伸展作用时期, 由此引发基底广泛熔融、北淮阳剧烈火山喷溢以及合肥盆地断陷等过程. 这次热窿事件包括强热伸展阶段(140~105 Ma)和晚期衰退阶段(105~85 Ma). 在强热伸展阶段, 热窿区集中在大别/红安地块, 并以大别地块热窿作用最为强烈, 某种意义上可将红安地块视为大别热窿翼部. 大别热窿构造包括麻城-岳西热轴、穹核组合及其外围翼部, 热轴平行造山带延向展布, 北淮阳与高压/超高压(HP/UHP)岩石构成下滑翼部, 岳西、罗田热窿核分别占据麻城-岳西热轴东、西两端, 它们最终成型时代不一致. 热窿伸展进程伴随着大别强热中心沿着麻城-岳西热轴迁移摆动, 如果将热窿事件与造山带根部拆沉相联系, 这种拆沉作用应以间歇性、迁移性、规模性与区域差异性为特征. 在伸展衰退阶段(105~85 Ma), 大别造山带仍显现穹状热窿布局, 但其伸展强度、岩浆活动显著减弱. 解析热窿伸展作用, 对于认识大别HP/UHP岩折返模型、"南北大别界限"以及造山过程机理具有重要启示意义.     

HYDROPOWER RESOURCES IN UPPER YANGTZE

ＨＹＤＲＯＰＯＷＥＲＲＥＳＯＵＲＣＥＳＩＮＵＰＰＥＲＹＡＮＧＴＺＥＣＨＥＮＪｉｓｈｅｎｇ（ＤｉｒｅｃｔｏｒＥｍｅｒｉｔｕｓ，ＹａｎｇｔｚｅＲｉｖｅｒＳｃｉｅｎｔｉｆｉｃＲｅｓｅａｒｃｈＩｎｓｔｉｔｕｔｅ（ＹＲＳＲＩ），２３ＨｕａｎｇｐｕＲｏａｄ，Ｗｕ...     

现代大地构造理论中的某些重要问题

现状和问题现代大地构造中的问题与过去一样还没有完满地解决.其原因是缺乏能满足重大科学要求的统一的理论,因而对一直存在的大大小小的问题采用了各种不同的解决方式.在这种情况下自然更难以找到一个重要的基础或基点作为研究的起点,以致密观上使人们无所适从,并对大地构造学作了显然是虚无主义的评价.最近二十年,地质科学引入了大量精确的地球物理学概念,成功地解释了现代大洋的一系列特征,还引入了新全球构造说或岩石圈板块说.最初,似乎终于找到了解决所有构造学难题的灵丹妙药.全球构造是以行星演变的动力学和物质统一性为基础,对构造作用和现象     

新疆塔里木盆地西部晚白垩世—早第三纪的沟鞭藻及疑源类

一、前言 新疆塔里木盆地西缘上白垩统一下第三系含有丰富的沟鞭藻及疑源类,此外多种无脊椎动物化石也很丰富。余静贤和张望平已对该区晚白垩世的沟鞭藻和疑源类作过初步研究。郝诒纯和曾学鲁等则对该区晚白垩世—早第三纪的有孔虫作了详细的研究。     

О ГЕОЛОГИЧЕСКОМ СТРОЕНИИ И РАЗВИТИИ ОКЕАНИЧЕСКИХ ВПАДИН

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