Multivariate discriminant analysis distinguishes metal- from non metal-related biomarker responses in the clam Chamaelea gallina

Molecular biomarkers are among the most sensitive and earliest responses to pollutants. However, lack of detailed knowledge on variability of responses and their possible seasonal variation limit their use. In addition, the seasonality of biological processes modulates the response of organisms to pollutant stressors. Using multivariate statistics, we have studied the influence of environmental and biological factors on the response of a battery of molecular biomarkers in the clam Chamaelea gallina collected along the South-Spanish littoral. Multivariate discriminant analysis clearly distinguished biomarker response between clean and polluted areas, using heavy metals as indicator of pollution. Such differences disappeared when the dataset was normalised for metal content, thus indicating that pollution was the main significant cause of the changes observed between clean and polluted sites. In conclusion, this work shows that, when applying a complete biomarker panel, multivariate statistical tools can be used to discern pollutant- from non pollutant-related responses.     

马厂箐铜矿床黄铁矿流体包裹体He-Ar同位素体系

对马厂箐斑岩型铜矿黄铁矿中流体包裹体的氦、氩同位素研究表明,在~3He/~(36)Ar-~(40)Ar/~(36)Ar和~(40)Ar/~4He-Rc/Ra空间,流体包裹体的氦.氩同位素组成呈明显的正相关.由这种相关性确定出,成矿流体为地壳和地幔两个端元流体的混合物.其中,前者为富含地壳氦但具空气氩同位素组成特征、贫硫和碳等挥发份的大气成因低温地下水,后者为由形成马厂箐富碱斑岩体之壳慢混合岩浆分异出的富含硫和碳等挥发份的高温岩浆流体.     

Application of trichloroacetic acid (TCA) to extraction of soft body for the determination of tissue Cd, Cu, Pb and Zn in the prosobranch Hydrobia ulvae (Pennant)

The application of trichloroacetic acid (TCA) as a shell extractant for preparation of soft body parts with reference to tissue metal concentrations (Cd, Cu, Pb, Zn) in shellfish has been evaluated on the example of the mud snail Hydrobia ulvae, a small marine prosobranch densely present in rocky and soft-bottom habitats of the eastern Atlantic. A solution of 0.1 M TCA was tested on individuals treated according to two different protocols: (1) thawed after freezing ("non-dried") and (2) thawed and air-dried to a constant weight ("dried"). Two points were investigated in detail to improve the method: individual soft tissue dry weight and tissue metal concentration following a standard digestion method. In both instances, the results were compared with those from manually dissected snails. Conditions for total shell decalcification of 60 individuals (3-4 mm long) were 5.5 h in 20 ml of 0.1 M TCA.No differences in individual soft tissue weight were observed between the treatments, indicating good efficiency of the TCA extraction with respect to weight of soft body parts. In contrast, tissue metal concentrations varied among treatments. The TCA extraction of the dried animals had a good recovery for Cd, most likely due to the lower solubility of Cd vital cellular components (proteins and mineral concretions) from the dried tissue. Satisfactory recoveries of the tissue concentrations of Cu and Pb were obtained for the non-dried individuals. This might be related to the specific distribution of metals in the organism (namely in the digestive glands and gonads) and their different chemical reactivity with TCA after the tissue was dried. Limited susceptibility of Zn-bearing protein bindings to complexing with TCA also accounts for significantly lower concentrations of Zn in the mud snail's soft tissue that was extracted. The 0.1 M TCA solution is therefore recommended for extraction of the shells of Hydrobia ulvae for tissue determination of Cd, Cu and Pb; however the treatment protocol does affect metal recovery and thus a consistent procedure should be followed.The extracted metals from the soft tissues and shells of the mud snails (on the basis of both metal concentrations and contents) were ranked in order of increasing contribution of soft body parts to the total (shell+tissue): Pb相似文献   

Transport of three herbicides in ground water at Twin Lake test site,Chalk River,Ontario, Canada

A field tracer test performed under natural flow conditions at the Twin Lake test site, Chalk River Laboratories of the Atomic Energy of Canada Ltd. in Chalk River, Ontario, Canada, using tritium and three herbicides (Chlortoluron, Terbuthylazine, and Pendimethalin) was interpreted using the dispersion equation with a combined reaction model. The reaction model couples an instantaneous equilibrium reaction governed by a linear adsorption isotherm with a reversible or irreversible kinetic reaction of the first order, and decay. An improved interpretation method consists of a simultaneous fitting of theoretical concentration and mass-recovery curves to the experimental data, which leads to a more reliable determining of reaction models and improves the accuracy of fitting. Tritium served as the reference tracer to determine the flow velocity, dispersivity, and the recovery of the herbicides. Chlortoluron was slightly delayed by equilibrium exchange with strongly reduced concentration due to an irreversible kinetic reaction and/or decay. Terbuthilazine was slightly delayed by equilibrium exchange, with strongly reduced concentration due to a reversible kinetic reaction with some influence of decay. A strong equilibrium reaction and a strong reversible kinetic reaction without degradation governed the transport of Pendimethalin, reducing considerably its concentration. The results obtained show that simulations based only on Kd and decay constant, especially if these parameters are found in the laboratory, may considerably differ from those performed with reaction parameters determined in properly performed field tests. The dominant reaction types, and the values of parameters found in the study, supply useful information on the transport of the investigated herbicides in sandy aquifers under natural flow conditions.     

2004年12月26日苏门答腊—安达曼地震激发的地球自由振荡

根据300~500S的面波估计得到的哈佛大学矩心矩张量断层机制,当周期大于1000S时,地球的地震自由振荡的振幅将异常地大。我们对一个比较陡的断层应用根据地震的体波和面波得到的更真实的破裂模型,估计了自由振荡的振幅近似等于地震矩(6.5×1022N.M),这相当于矩震级为9.15。由于破裂的持续时间达600S,故断层破裂模型足以描述地震的观测结果,但却低估了大地测量位移,这些位移表明在尼科巴群岛和安达曼群岛下面发生了缓慢的断层运动。     

Adsorption of As on hydroxy-Fe-montmorillonite complexes

DOI: 10.1360/03yd0553 Arsenic, a toxic element, is ubiquitous in the earth’s crust and may lead to health risks for humans. This may come about as a result of oxidative weathering and dissolution of As-containing minerals, use of ar-senical pesticides, excess use of some fertilizers and from mine drainage, smelter wastes and agricultural drainage water from certain arid regions. The dis-solved inorganic arsenic is transported in surface or2156 Science in China Ser. D Earth Sciences groun…     

European retirees on the Costa del Sol: a cross-national comparison

"This paper studies northern European retired immigrants, using data generated by a questionnaire survey of 300 respondents and in-depth interviews with 20 people in 13 municipalities. Reasons for moving to Spain, the advantages and disadvantages reported of living there, and the perceived impacts on the local region were analysed, paying particular attention to cross-national differences between British, German, Benelux and Nordic retirees. The results show that sociodemographic and economic features of the settlers tend to be quite similar, whilst reasons for moving, stated advantages and disadvantages, and consequences and impacts exhibit some cross-national differentiation."     

Some experiments with multivariate objective analysis scheme of heights and winds using optimum interpolation

A two-dimensional, multitvariate objective analysis scheme for simultaneous analysis of geopotential height and wind fields has been developed over Indian and adjoining region for use in numerical weather prediction. The height-height correlations calculated using daily data of four July months (1976-1979), are used to derive the other autocorrelations and cross-correlations assuming geostropic relationship. A Gaussian function is used to model the autocorrelation function. Since the scheme is multivariate the regression coefficients (weights) are matrix.Near the equator, the geostrophic approximation relating mass and wind is decoupled in a way similar to Bergman (1979). The objective analyses were made over Indian and adjoining region for 850, 700, 500, 300 and 200 hPa levels for the period from 4 July to 8 July 1979, 12 GMT. The analyses obtained using multivariate optimum in-terpolation scheme depict the synoptic situations satisfactorily. The analyses were also compared with the FGGE ana-lyses (from ECMWF) and also with the station observations by computing the root mean square (RMS) errors and the RMS errors are comparable with those obtained in other similar studies.